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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for 8' Bromo 2' 3' dihydro 1'h spiro cyclopropane 1 4' isoquinoline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and DAPI (4’,6-diamino-2-phenylindole) (D9542; Sigma; 1 μg/mL). Additionally ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Sigma H0887). These cells were chosen as an experimental system as previously described (Massacci et al. ...
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bioRxiv - Cell Biology 2024Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI) (1:10000, D8417, Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... followed by 2 h incubations with primary antibodies directed either against VAPA (Millipore, 1:200), CERT (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... 3 μl of the reducing reagent (mixture of 0.2 g 1-amino-2-naphtol-4-sulfonic acid (Millipore-Sigma, Burlington, MA, USA, #08751) with 0.2 g sodium bisulfite (Millipore-Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... in TNT for 2-3 h at room temperature and incubated with an anti-Fluo-POD antibody (1/50, Sigma-Aldrich REF 11 426 346 910) in 2% TNB overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM 4-(2-Aminoethyl)-benzenesulfonylfluoride hydrochloride (AEBSF) and 1 complete ULTRA protease inhibitor tablet (Sigma-Aldrich)) then clarified by centrifugation at 14,000 x g for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... and 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)) with 1 mM Dithiothreitol (DTT) (Sigma-Aldrich), and 5 mM EDTA (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... for 3-4 hours> Primary antibodies: Rabbit-α-GluA1 (Sigma-Aldrich AB1504, 1:300) and Mouse-α-bassoon (Abcam ab82958 ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies at RT for 2 hours with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542). Afterward ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1% eosin (H&E) (Sigma). For immunostaining ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 3-methyl-1-butanethiol (Sigma W385808), 1% octanal (Sigma O5608) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were then washed for 3 × 1 h in 1x PBS with 0.02% NaN3 (Sigma Aldrich – 58032) at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... selected for 2-3 weeks with 1 µg/ml of puromycin (Sigma- Aldrich), and surviving cells were fixed with methanol and stained with Giemsa (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich) added fresh] or RIPA buffer [25 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... and (3) GluA2 (mouse anti-glutamate receptor 2; Millipore; used at 1:2000). Cochlear pieces were incubated in species appropriate secondary antibodies coupled to Alexa Fluors in the red ...
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bioRxiv - Cancer Biology 2021Quote: ... or 3-(1,3-Benzodioxol-5-ylmethylene)-2-oxo-1-pyrrolidinecarboxaldehyde (KNK437, Sigma Aldrich) for 24 hours prior to collection of cells for RNA extraction.
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bioRxiv - Biochemistry 2022Quote: ... 2.5mM Na3VO4 and 1% each of phosphatase inhibitor cocktails 2 and 3 (Sigma) and set on ice for 10 minutes prior to sonication ...
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 3:2:1 in HEK 293T cells (Sigma Aldrich) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM PMSF and phosphatase inhibitor cocktail 2 and 3 from Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 to 3 million cells were cross-linked using 1% formaldehyde (Sigma, F8775) for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% each phosphatase inhibitor cocktails 2 and 3 (Sigma Cat #P5726 and P0044). Lysates were cleared at 17,000g ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM PMSF and phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich, Switzerland). The insoluble fraction was then sonicated using a fine probe 15 times at 0.5 sec pulse at an amplitude of 20% ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1 mM EDTA) supplemented with phosphatase inhibitor cocktail 2 and 3 (Sigma), and protease inhibitor cocktail (cOmplete Protease Inhibitor ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos were first treated with 10 mM 5-bromo-2’-deoxyuridine (BrdU, Sigma-Aldrich) pulses at designated stage and then fixed with fresh 4 % paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2021Quote: Mice received three intraperitoneal injections (100mg/kg) of 5’-bromo-2 deoxyuridine (BrdU, Sigma) at 4 h intervals and were sacrificed after 28 days ...
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bioRxiv - Neuroscience 2021Quote: Split-Cre mice received drinking water containing 5-bromo-2’-deoxyuridine (BrdU) (Sigma-Aldrich) (1 mg/ml ...
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bioRxiv - Genetics 2023Quote: ... B cells were incubated with 60 µM of 5-Bromo-2’-deoxyuridine (BrdU, Sigma) as indicated in the figure legends ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.015 mg/ml 5-bromo-2’- deoxyuridine (BrdU; Sigma Aldrich, St. Louis, MO, USA), 1.0 mM sodium pyruvate (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... Neurogenesis in the brain was accessed by injecting BrdU (5-Bromo-2′deoxyuridine, Sigma) for 6 days post ICI-treatments ...
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bioRxiv - Genetics 2024Quote: ... the cells were treated with 20 µM of 5-bromo-2’-deoxyuridine (BrdU, Sigma) at 4 hours after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... SYSY), vesicular glutamate transporter 2 (VGLUT2; 1:1,000, SYSY) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, 1:50,000, Millipore) at 4 °C for 16 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: PESTANAL® analytical grade tefluthrin (2,3,5,6-tetrafluoro-4-methylbenzyl 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylic acid) was purchased from Sigma Aldrich. Tefluthrin stock solutions were prepared in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... 3-(2-Methyl-5-nitro-imidazol-1-yl)-N-(2,2,2-trichloro-1-phenylamino-ethyl)-propionamide) (Sigma-Aldrich SML1503). Drugs were diluted in culture media at treatment.
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS and 3-(4,5-dimethyl-2-thiazolyl)2,5-diphenyl-2-H-tetrazolium bromide (MTT, Sigma-Aldrich) solution (final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Resulting supernatants were incubated with anti-FLAG M2 magnetic beads (Sigma, 3 h at 4°C). Beads were washed thrice with TUBE lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... or 3-octanol (Sigma, 8 µl in 8 ml mineral oil) were used as olfactory stimuli ...
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bioRxiv - Cell Biology 2021Quote: ... blocked for 1 h in 1% BSA (Sigma, Cat# A7906)/0.1% Triton X-100/PBS (blocking solution) ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h or with 0.3/1 µM staurosporine (Sigma). Cell viability was analyzed in 48 h after stress induction.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 2 mM of 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC, 22980-Sigma-Aldrich, St. Louis, MO, USA) and 5 mM of N-hydroxysulfosuccinimide sodium salt (NHS ...
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bioRxiv - Cancer Biology 2020Quote: ... were incubated for 1 h at RT and after washing coverslips were incubated for 4 min with DAPI (1:4000, Sigma-Aldrich #D8417) and mounted with ProLong Gold Antifade reagent (Invitrogen #P10144).
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bioRxiv - Cell Biology 2023Quote: ... cells were harvested on ice and lysed by rotation for 1 h at 4°C in JIES buffer (see above) containing 1× SIGMAFAST protease inhibitor cocktail (Sigma-Aldrich, S8830). Subsequently ...
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bioRxiv - Genetics 2024Quote: ... in TBS-T at room temperature for 1 h and incubated at 4 °C overnight with mouse-anti FLAG (1:400, Sigma-Aldrich, F3165) or mouse-anti Cas9 (1:1,000 ...
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bioRxiv - Microbiology 2020Quote: ... 1 µl of matrix (α-Cyano-4-hydroxycinnamicacid, HCCA, CAS Number 28166-41-8, Sigma-Aldrich, Switzerland) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 3-4 h at 4 °C with 15-20 μL of ANTI-FLAG® M2 Affinity Gel (Sigma) and washed 3-4 times with Co-IP wash buffer containing 0.1% Triton-100 but without Phosphatase Inhibitor Cocktails and Protease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5mM Bromo-UTP (Sigma B7166), 1% Na-laurylsarcosine ...
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bioRxiv - Developmental Biology 2021Quote: Fish with mutations and transgenic background at 3.2 or 4 dpf were fixed 1 to 2 days at 4℃ with 4% paraformaldehyde (Sigma, P6148) in PBS ...
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bioRxiv - Immunology 2020Quote: ... HEK293T cells were transduced at MOI 2:1 with 8 μg/mL of polybrene (Sigma-Aldrich). One day before transduction ...
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bioRxiv - Neuroscience 2020Quote: ... were performed with MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in Sanz et al ...