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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for 8' Bromo 2' 3' dihydro 1'h spiro cyclopropane 1 4' isoquinoline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... two siRNA sequences targeting different regions of NOK were designed as #1: 5’-GCAAGAAACAUUCAUGCAU-3’ and #2: 5’-GUCUUUCCCAGGGACACAA-3’ (SASI_Hs01_00043410 and SASI_Hs02_00351937, Sigma, St. Louis, MO, USA). The control siRNA is a scrambled sequence (5’-UUCUCCGAACGUGUCACGUdTdT-3’ ...
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bioRxiv - Immunology 2022Quote: ... for 2 h at room temperature and then blocked for 1 h at room temperature with 5 % neonatal calf serum (Millipore Sigma, St. Louis, MO) in PBS ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then washed twice with PBS prior to 20 min permeabilization with 0.5% Triton X-100 and 1 h blocking with 3% BSA (Sigma) at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... washed with PBS and blocked at room temperature for 1 h using either 3% bovine serum albumin (BSA, Sigma) or 5% goat serum according to the primary antibody used ...
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bioRxiv - Microbiology 2020Quote: ... After 23 h, cells were fixed with 4% paraformaldehyde and stained with DAPI (4’, 6-diamidino-2-phenylindole) (Sigma-Aldrich, USA). Nine fields per well were imaged on an Operetta high-content imaging system (PerkinElmer) ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (1:3000; Sigma-Aldrich) and coverslips were mounted with FluoroGel (Electron Microscopy Services ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1:10000; Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2021Quote: DOI or 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane was obtained from Sigma-Aldrich. Vehicle refers to saline (0.9%) ...
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bioRxiv - Cell Biology 2020Quote: ... 4′,6-diamidino-2-phenylindole (DAPI, Sigma #D9542, 1:1000 for 10min at RT) Mice were deeply anesthetized with isoflurane and then transcardially perfused with ice-cold heparinized PBS (25U/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... slices were incubated with 4′,6-diamidino-2-phenylindole (DAPI; 1:500, Sigma-Aldrich) to counterstain cell nuclei ...
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bioRxiv - Neuroscience 2022Quote: ... After staining nuclei with DAPI (1:1000, 4’, 6-diamidino-2-phenylindole dihydrochloride; Sigma) and several washes with PBST ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (1:3000; Sigma-Aldrich), and coverslips were mounted with FluoroGel (Electron Microscopy Services ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for 1 hour at 4 °C with 2% rat serum (Sigma) to reduce antibody binding to Fc receptors ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (1:3000; Sigma-Aldrich) and coverslips were mounted with FluoroGel (Electron Microscopy Services ...
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bioRxiv - Molecular Biology 2024Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES buffer) were purchased from Sigma Aldrich. 20 mg/mL of protein was mixed in 50 mM HEPES at pH 7.1.2 M ammonium sulphate in 0.1 M Tris-HCl at pH 8 was used as the precipitant solution.41 Crystallization trials were set up similar to lysozyme.
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bioRxiv - Microbiology 2023Quote: ... 8 μL of 1 mg/mL PEI (Sigma), 0.8 μg LentiCRISPR-V2-gRNA plasmid ...
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bioRxiv - Biochemistry 2023Quote: ... 8-Br-cGMP (1 mM) (B1381, Sigma Aldrich) and adenosine 5’-diphosphate sodium salt (ADP ...
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bioRxiv - Biochemistry 2023Quote: ... and 8 μg ml−1 RNase (Sigma-Aldrich) supplemented with 1% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... The immune complexes were collected by incubation (2 h, 4°C) with protein G-agarose (Sigma). Co-IP assays were performed by using Pierce™ Protein G Agarose (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... For Diethyl-1,4-dihydro-2,4,6-trimethyl-3,5-pyridindicarboxylat (DDC, 137030 Sigma-Aldrich)-induced liver injury ...
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bioRxiv - Cancer Biology 2020Quote: ... and active caspase-3 (1:1 000, Millipore, AB3623). Three fields of view for each slide were imaged using an Olympus BX43 Upright Microscope and cells were manually counted and scored using ImageJ Software.
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bioRxiv - Biophysics 2022Quote: ... 250 μM 3-isobutyl-1-1-methylxantine IBMX (Sigma), 100 nM Cortisol (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM 3-Isobutyl-1-methylxanthine (IBMX, Sigma) in PBS for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... DNA damage was induced where indicated by treatment with 2 μg/ml 4-Nitroquinoline 1-oxide (4-NQO) (Sigma) for 60 min before harvesting ...
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bioRxiv - Molecular Biology 2021Quote: ... Brdu-ChIP was performed after addition of 20 μM BrdU (5-bromo-2′-deoxyuridine, Sigma Aldrich) directly to HeLa culture medium for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Senescence was induced by treating cells with 100 µM 5-Bromo-2’-deoxyuridine (Sigma Aldrich, USA) for 48 hours ...
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bioRxiv - Neuroscience 2023Quote: ... we injected 5-bromo-2′-deoxyuridine (BrdU, 10 mg/ml in saline, 100 mg/kg, Sigma) through an intraperitoneal (i.p. ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Hydroxytamoxifen (4-HOT) (H-7904, Sigma, St. Louis, MO), the active metabolite of tamoxifen ...
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bioRxiv - Microbiology 2021Quote: ... Buffer exchange into TBS pH7.4 and 1 mg/ml Amphipol 8-35 using Amicon Ultra-4 centrifugation filters with a 100kDa cutoff (Millipore). The concentration of Amphipol 8-35 was brought up to 35mg/ml and incubated at room temperature for 4 hours then Bio-Beads SM-2 (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total of 150 µL of 3% 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (oxazolone; Sigma-Aldrich) dissolved in 100% ethanol (Wako ...
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bioRxiv - Cell Biology 2023Quote: ... was used at 1 µM and ARP 2/3 complex inhibitor - CK666 (182515; Sigma-Aldrich) was used at 100 µM concentration ...
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bioRxiv - Neuroscience 2022Quote: ... and 1% each of Phosphatase Inhibitor Cocktails 2 and 3 (Millipore Sigma #P5726 and #P0044). Cells were scraped ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1% each of phosphatase inhibitor cocktails 2 and 3 (Sigma, Saint Louis, MO, USA). Particulate was removed by centrifugation of lysates at 13,000 rpm for 10 minutes at 4°C and filtration through 0.45 µm syringe filters and lysates were stored at -80°C until preparation for MIB-MS ...
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bioRxiv - Cell Biology 2023Quote: ... plates were treated with 3-amino-propyltrimethoxysilane (diluted 1:2 with PBS; Sigma-Aldrich/Merck) for 15min at RT ...
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bioRxiv - Bioengineering 2024Quote: ... spinal cords were subjected to 2-3 incubation periods with DNase (1 mg/mL, Sigma) and the fragments were mechanically digested ...
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bioRxiv - Microbiology 2020Quote: ... primary antibodies were mouse-anti-ROP2/3/4 (1:250 dilution, 4A7, (58)) and rat anti-HA (1:500, clone 3F10, Sigma Aldrich, 11867431001), and secondary antibodies were goat anti-mouse IgG-Alexa 647 (1:2000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... 3- [(3- Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS, Sigma RES1300C), digitonin (Sigma D141) ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/ml 3′,3′-diaminobenzidine tetrahydrochloride hydrate (Sigma), 24 units/ml catalase from bovine liver (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... 20 h without IAA and 7 h with 1 μM 4OHT (Sigma SML1666) (Dam-H3K27me3-mintbody) ...
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bioRxiv - Cell Biology 2024Quote: ... DLD1 cells were treated for 2 h with 0.5 mM 3-indoleacetic acid (Sigma, stock in ethanol). At the time points indicated ...
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bioRxiv - Cell Biology 2022Quote: ... diluted with fresh medium (1/2) and hexadimethrine bromide was added (final concentration 8 μg/ml; Sigma-Aldrich). Target cells were then infected ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours ...
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bioRxiv - Cell Biology 2022Quote: ... 1 × 105 Cas9 TNG MKOS MEFs were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 polybrene (Merck-Millipore) for 4 hours ...
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... 100 mM KCl and pre-loaded with 0.4 mM substrate (Gdm+ or guanylurea) and 1mM pyranine (trisodium 8-hydroxypyrene-1, 3, 6-trisulfonate; Sigma-Aldrich) using three freeze/thaw cycles ...
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bioRxiv - Genetics 2021Quote: ... and 0.1% (w/v) SDS) containing 1× homemade protease inhibitor cocktail (1 mM 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (Sigma; A8456), 0.3 μM Aprotinin ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:1 mixture of 4% HMS (2% final concentration) and 20% bovine serum albumin (BSA; 10% final concentration; V900933, Sigma), incubated at 4°C for 1 week ...
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bioRxiv - Cell Biology 2020Quote: ... For AsiSI or I-PpoI nuclease induced DSBs, cells were treated with Shield-1 (1 μM, AOB1848, Aobious) and 4-OHT (2 μM, H7904, Sigma) for 5 hours before washing with PBS and adding back media.
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bioRxiv - Plant Biology 2021Quote: ... 1 g of the infiltrated leaf tissue was ground in 4 mL of extraction buffer (1/2 tablet of Sigma Complete™ mini protease inhibitor (per 15 mL) ...
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bioRxiv - Cell Biology 2023Quote: ... adipocytes were lysed in polysome lysis buffer (20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl2, 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma] ...
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bioRxiv - Physiology 2022Quote: The membrane-permeable 8-Br-cGMP (8-bromoguanosine 3′,5′-cyclic monophosphate, B1381) and 8-Br-cAMP (8-bromoadenosine 3′,5′-cyclic monophosphate, B5386) were from Sigma. ANP (AS-20648) ...