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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for 8' Bromo 2' 3' dihydro 1'h spiro cyclopropane 1 4' isoquinoline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... fixed for 2 h in formaldehyde solution 4% buffered to pH 6.9 (Sigma-Aldrich, #100496), and analyzed for morphogenetic defects ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mM 3-isobutyl-1-methylxanthine (IBMX, Sigma), 1 mM DTT (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... 4 μL of Cell Counting Kit-8 (CCK-8; Sigma-Aldrich) was added to each well ...
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bioRxiv - Developmental Biology 2020Quote: ... paws were incubated twice for 5min with methanol 50% BABB (1/3 benzylalcohol and 2/3 benzylbenzoate, from Sigma), and then three times in pure BABB for 5min each (or until the sample is cleared) ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-Bromo-2′-deoxyuridine (10280879001) and D-Luciferin (L9504-5MG) was purchased from Sigma-Aldrich. All plasmids used in this study were listed in supplementary Table 1 and will be donated to Addgene.
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bioRxiv - Microbiology 2023Quote: ... Parasites were incubated with 100 μM of 5-bromo-2-Deoxyuridine (BrdU, Sigma Aldrich B5002) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Senescence was induced by treating HeLa cells with 200μM 5-Bromo-2’-deoxyuridine (BrdU, Sigma) freshly prepared in DMSO or exposing the cells to ionizing radiation (8 Gy ...
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bioRxiv - Bioengineering 2021Quote: ... 48 h and day 21 hBMMSC-seeded scaffolds (n=3) were fixed in 4% paraformaldehyde (Sigma, Canada) for 1 hour and submerged in gradient sucrose solutions from 10% to 30% ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 24-48 h with the virus supernatant supplemented with 8 µg ml-1 polybrene (Sigma-Aldrich #H9268) before being subjected to further experimental procedures.
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bioRxiv - Immunology 2021Quote: ... Cultured BMDMs were stimulated with heat-killed KP (MOI = 10) up to 8 h or Klebsiella LPS (1 ug/ml) (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were transduced for 48 h in 6 well plate in 1 ml of medium containing 8 µg/mL of polybrene (Sigma-Aldrich). VHL-NbGFP4-FLAG HelaS3 cell line was obtained after selection with 1 µg/mL of puromycin (Invivogen).
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bioRxiv - Genomics 2022Quote: ... K562 or Jurkat cell lines expressing CRISPRi effectors of interest were infected with lentivirus containing sgRNA expression vectors by centrifugation at 1000 × g for 1 h in 24-well plates in the presence of 8 µg/mL polybrene (Sigma-Aldrich). RPE1 ...
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bioRxiv - Systems Biology 2022Quote: ... and phosphatase inhibitor cocktail 2 and 3 (1:100, P5726 and P0044, Sigma-Aldrich). For immunoprecipitation (IP) ...
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bioRxiv - Immunology 2020Quote: ... and cyclophosphamide (#C7397, 100 mg kg-1 from day −3 to −2; Sigma-Aldrich) intraperitoneally ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM Benzamidine) containing phosphatase inhibitor cocktail 2 and 3 (Sigma, P5726 and P0044). Supernatants containing proteins were collected after centrifugation at the highest speed for 15 minutes at 4°C ...
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bioRxiv - Physiology 2024Quote: ... 1% phosphatase inhibitor cocktails #2 and #3 (Sigma-Aldrich; Catalog no.: P0044 and P5726), one cOmplete protease inhibitor tablet (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 1% (v/v) of 1 M HEPES (Sigma-Aldrich, H-4034) at Ph 7.2–7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at RT and with DAPI (1/20,000; Sigma) for 5 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... 14-3-3 (Millipore AB9748-I, 1:2000); TOPO II (Abcam 109524 ...
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bioRxiv - Bioengineering 2022Quote: ... 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma) and N-hydroxysulfosuccinimide (Sulfo-NHS ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: The 5-HT2A antagonist (R)-(+)-α-(2,3-Dimethoxyphenyl)-1-[2-(4-fluorophenyl)ethyl]-4-piperinemethanol (M100907; Sigma Aldrich) at a dose of 0.5 mg/kg.
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bioRxiv - Cell Biology 2023Quote: ... plus cyclosporine-H (1 μM, Sigma-Aldrich) by confocal microscopy using Operetta CLS microscope (PerkinElmer) ...
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bioRxiv - Genetics 2024Quote: ... for 1 h and DAPI (Sigma-Aldrich) (1:1,000 ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1 µM cyclosporine-H (Sigma-Aldrich) in the Operetta CLS microscope (30 min at 37 °C in a 5 % CO2 atmosphere ...
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bioRxiv - Plant Biology 2023Quote: ... incubated 1 h in Streptavidin-HRP (Sigma) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 h or with sorbitol (Sigma) for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... confluent EnSC monolayers were down-regulated in phenol-free DMEM/F□12 media containing 2% DCC□FBS and decidualized with 10 μM medroxyprogesterone acetate (MPA) and 0.5 mM 8 bromo cAMP (C+M treatment) (Sigma Aldrich). To study the effect of exogenous HA ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed with PBST and then incubated for 2 h at room temperature in PBS with 2 μg mL−1 DAPI (D9542, Sigma-Aldrich). Cells were imaged using an LSM980 confocal microscope (Zeiss) ...
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bioRxiv - Immunology 2024Quote: ... adhered cells were incubated for 2 h at 37°C with 100 µL of 1% p-nitrophenylphosphate (2 mg/mL, Sigma-Aldrich) in AMP buffer (0.1 M ...
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bioRxiv - Plant Biology 2024Quote: ... The tissue was placed in 99.8% ethanol and LR White resin (at a ratio of 3:1, 1:1, 1:3; Sigma Aldrich, USA), and next in 100% resin LR White ...
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bioRxiv - Molecular Biology 2022Quote: ... 4-nitroquinoline 1-oxide (4-NQO) (Sigma). Treatment conditions are indicated in the corresponding figures ...
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bioRxiv - Microbiology 2021Quote: ... 1 µM 4-hydroxytamoxifen (4-OHT; Sigma) for 1h at 37°C prior to infection and during the course of infection ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Cell Biology 2021Quote: ... transduction of cells was performed with a multiplicity of infection equal to 3 in the presence of 8 µg/ml Polybrene (H-9268; Sigma-Aldrich). Cells were then selected with 2.5 µg/ml puromycin for 2 days ...
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bioRxiv - Cell Biology 2023Quote: ... Medium samples from day 2 and day 4 had to be diluted 1:1 in PBS (P4417, Sigma-Aldrich) because of the higher levels of cell death in the first days after harvesting caused by the isolation procedure ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at room temperature or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at RT or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Biochemistry 2021Quote: ... and soluble protein was clarified by centrifugation (50,000×g, 1 h, 4°C) and subsequently passed through a 0.22-μm filter (Merck Millipore).
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with agitation for 1 h at 4 °C with 250 IU of benzonase (#70746, Millipore) followed by 15 min centrifugation at 15,000 x g at 4°C to remove the insoluble fraction of the lysate ...
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bioRxiv - Biochemistry 2024Quote: ... cell pellets were collected and lysed at 4 °C for 1 h in 1x PBS+1% Tritonx-100+1x Complete protease inhibitor (Sigma-Aldrich, cat. no. 11697498001), and centrifuged at > 20,000x g at 4 °C for 10 min ...
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bioRxiv - Biochemistry 2024Quote: Cell pellets were collected and lysed at 4 °C for 1 h in 1x PBS+1% Tritonx-100+1x Complete protease inhibitor (Sigma-Aldrich, cat. no. 11697498001). The lysates were then centrifuged at 12,000x g at 4 °C for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... were resuspended 2 mL of viral particle supernatant for E:T of 2:1 with 8 μg/mL of Polybrene (Millipore Sigma, Burlington, MA), maintained in at 37°C for 4 hours ...
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bioRxiv - Biophysics 2022Quote: ... HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl] ethanesulfonic acid) was purchased from EMD Millipore (Billerica, MA USA) and NaCl (sodium chloride ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 mg total protein were mixed with 2 mL Urea Buffer (UB) (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.0 and 50mM ammonium bicarbonate) ...
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bioRxiv - Neuroscience 2024Quote: ... 8-cyclopentyl-1,3-dimethylxanthine (CPT; 3 nmol in 5 μl saline of 2% DMSO; #C102; Sigma-Aldrich; injection was given immediately before the start of exposure to restraint stress or 30 min before intrathecal injection of NA).
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bioRxiv - Physiology 2020Quote: ... 3-isobutyl-1-methylxanthine (Sigma); Glyh-101 (a gift from the Cystic Fibrosis Foundation Therapeutics and Robert Bridges ...
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bioRxiv - Pathology 2021Quote: ... 1% phosphatase inhibitor 3 (Sigma), and 1X HALT protease inhibitor cocktail (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Odors (Sigma, Tables 1-3) were injected into the steady air stream by opening valves to one of two odor reservoirs ...
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bioRxiv - Plant Biology 2021Quote: ... antibodies followed by a 2 h incubation in secondary goat anti-mouse-HRP (1:10,000 dilution; Millipore). Detection was performed using the SuperSignal West Femto Detection Kit for anti-GFP ...
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bioRxiv - Developmental Biology 2022Quote: ... 2/3 mTeSR1 + 1/3 N2 medium + LSX + PSD Day 6,7: 1/3 mTeSR1 + 2/3 N2 medium + PSD At day 6 cells were detached by Accutase solution (Sigma-Aldrich) and incubated at 37°C for 20 minutes to obtain a single cell suspension ...