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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for 8' Bromo 2' 3' dihydro 1'h spiro cyclopropane 1 4' isoquinoline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and 1% phosphatase inhibitor cocktails 2 and 3 (Sigma). Extracts were sonicated 3 × 10 s ...
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bioRxiv - Neuroscience 2023Quote: ... 3) mouse IgG2a anti-GluA2 (Millipore, 1:2 000) and secondary antibodies AlexaFluor 647 donkey anti-rabbit ...
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bioRxiv - Cancer Biology 2024Quote: ... were washed two times with PBS and lysed by scraping into 500 μl per plate of lysis buffer (40 mM Tris/HCl pH 7.6, 8 M urea, EDTA-free protease inhibitor complete mini [Roche], and phosphatase inhibitor cocktails 1, 2, and 3 [Sigma-Aldrich] at 1× final concentration according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 3-isobutyl-1-methylxanthine (0.5 mM; Sigma, 28822-58-4), indomethacin (200 μM ...
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bioRxiv - Cell Biology 2022Quote: ... pH=8) and incubated for 1 h in a Western Blocking Reagent solution (Sigma-Aldrich). Membranes were incubated overnight at 4 °C with 20 ml of the respective primary antibody solution ...
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bioRxiv - Cell Biology 2020Quote: ... 5-bromo-2-deoxyuridine (BrdU) were acquired from Sigma Aldrich. Sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino ...
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bioRxiv - Pathology 2020Quote: ... and 5-bromo-2-deoxyuridine (BrdU, 100 μmol/L, Sigma). Prior to treatment ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by 100μL of 5-bromo-2’-désoxyuridine (BrdU, Sigma) at 10 mg/mL 90 minutes later ...
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bioRxiv - Immunology 2023Quote: Cell cycle analysis: 5-Bromo-2’-deoxyuridine (BrdU, Sigma Aldrich) staining was performed as described previously94.
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bioRxiv - Neuroscience 2022Quote: ... mice were given 5-Bromo-2’-deoxyuridine (BrdU) (Sigma, B5002). BrdU was dissolved in 0.9% saline at a concentration of 10 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... we used 5-bromo-2’-deoxyuridine (BrdU) (#B5002, Sigma-Aldrich), a thymidine analogue able to substitute thymidine in the DNA double chain synthesis occurring in proliferating cells (Kee et al. ...
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bioRxiv - Neuroscience 2024Quote: ... 5-Bromo-2’-deoxyuridine (BrdU; Sigma B5002; 0.8 mg/ml) was provided in the drinking water for a period of 2 weeks ...
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bioRxiv - Immunology 2021Quote: ... for 1 h and cultured for 4 h in the presence of 0.5μg/ml Brefeldin A (SIGMA), 0.005mM Monensin and 0.2μg/ml anti-CD107a-APC antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... medium changed to IMDM+10%FCS+Penicillin-streptomycin + 8-bromo-cAMP (10μM, Sigma-Aldrich), and then incubated overnight at 37°C 5%CO2 ...
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bioRxiv - Biochemistry 2022Quote: ... pLANT-2/RIL–RFC[1+5] was co-transformed with pET(11a)-RFC[2+3+4] into BLR(DE3) cells (Novagen, Madison, Wisconsin). The cell lysate was clarified by centrifugation ...
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bioRxiv - Genetics 2021Quote: ... Mouse neural progenitor cells were routinely passaged 1:2-1:4 every 3-5 days using Accutase and maintained in NS expansion medium on laminin-coated plates (Sigma, 3 μg/ml). Both mESCs and mNPCs were incubated at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... 4% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate (CHAPS; Sigma-Aldrich, Cat. No. 226947)] ...
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bioRxiv - Physiology 2021Quote: ... was incubated for 2-3 h at 4 °C with 30 µl of FLAG-conjugated beads (anti-DYDDDDDK beads, Sigma) or empty beads (ThermoScientific) ...
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bioRxiv - Neuroscience 2023Quote: ... GluR2/3: Anti-Glutamate Receptor 2 & 3 antibody (1:500, Millipore, Cat# AB1506). c-Fos ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 or 8 h with 500 µM Indole-3-Acetic Acid (IAA, Sigma-Aldrich #I5148). For induction of Oct4 knockdown in ZHBTc4 cells ...
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bioRxiv - Neuroscience 2020Quote: ... The signal was determined by nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) (BCIP) (Sigma, 72091).Semithin sections for toluidine blue myelin staining and ultrathin section for TEM imaging were prepared according to our previous protocols (Zhang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 60°C and developed using nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) (Sigma Aldrich) for 60 minutes ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of the DIG-labelled probes was carried out by using an anti-DIG antibody coupled with alkaline phosphatase and a ready-to-use 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro-blue tetrazolium (NBT) solution (Sigma-Aldrich) as the chromogenic substrate for the enzyme ...
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bioRxiv - Developmental Biology 2020Quote: ... The staining reactions lasted between 2h and 8h at room temperature using nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3′-indolyphosphate (BCIP) staining reactions (Sigma Aldrich). Embryos were then rinsed in PBS-T (0.05% Tween-20) ...
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bioRxiv - Plant Biology 2020Quote: ... and placed in Petri dishes filled with 0.5% agarose containing 35 mM β-mercaptoethanol (v/v) and 1.5 mg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Sigma). For X-gal staining ...
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bioRxiv - Microbiology 2022Quote: ... Detection was performed by colorimetry using nitro blue tetrazolium chloride / 5-bromo-4-chloro-3-indolyl-phosphate (NBT-BCIP, Sigma-Aldrich) as a substrate.
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked for 1 h in 3 % BSA (Sigma-Aldrich, A7906-100G) in TBS-T at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Samples were blocked for 1 h in 3% bovine serum albumin (Sigma-Aldrich) and subsequently incubated in primary antibodies (overnight ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were blocked for 1 h in 3% skim milk (Sigma□Aldrich) in 1x TBS (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... dihydro-β-erythroidine (dHβE, 50 μM, Sigma) and D-tubocurarine chloride (30 μM ...
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bioRxiv - Cell Biology 2024Quote: ... 4μM of dihydro cytochalasin B (DCB; Sigma).
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bioRxiv - Neuroscience 2021Quote: ... 8 uL 4-Hydroxy-TEMPO inhibitor (1:200 w/v, Sigma Aldrich, 176141), 8uL TEMED accelerator (10% v/v ...
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bioRxiv - Microbiology 2022Quote: ... Samples were diluted at least 1:4 in 8 M urea (Sigma-Aldrich) dissolved in 100 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... 8 uL 4-Hydroxy-TEMPO inhibitor (1:200 w/v, Sigma Aldrich, 176141), 8uL TEMED accelerator (10% v/v ...
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bioRxiv - Neuroscience 2024Quote: ... 8 uL 4-Hydroxy-TEMPO inhibitor (1:200 w/v, Sigma Aldrich, 176141), 8uL TEMED accelerator (10% v/v ...
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bioRxiv - Neuroscience 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; 1:1000; Sigma) was added to the cell suspension ...
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bioRxiv - Neuroscience 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma) was used to show nuclei clear and dyed for 5 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 4′,6′-diamidino-2-phenylindole (1:3000; Sigma-Aldrich) was used to counterstain sections and coverslips were mounted using Fluorogel l (Electron Microscopy Sciences ...
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bioRxiv - Genetics 2022Quote: ... Bb was cultured at 37ºC in Barbour-Stonner-Kelly with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer media (BSK-H) complete with 6% rabbit serum (Millipore Sigma) and 1% Amphotericin B (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... individuals were placed in a microtube containing in 100 µl of either Schneider’s media (Experimental Replicates 1 and 2) or RPMI media (Experimental Replicates 3 and 4) to which 2% Penicillin/Streptomycin and Amphotericin B (Sigma-Aldrich, Dorset, UK) had been added ...
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bioRxiv - Bioengineering 2023Quote: ... along with 5% 2-hydroxy-1-[4(hydyroxyethoxy)phenyl]-2- methyl-1-propane (Irgacure 2959; Sigma-Aldrich) photoinitation ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were sacrificed under anesthesia with 2-bromo-2-chloro-1,1,1-trifluoro-ethane (Sigma) by cervical dislocation ...
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bioRxiv - Microbiology 2024Quote: ... Labelling was performed at RT for 1 h and quenched with 8 μl 5% hydroxylamine (Sigma). Labelled samples were acidified by adding 50 μl 1% TFA ...
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bioRxiv - Neuroscience 2020Quote: ... and cargo in a 1:4:1 ratio with PEI reagent (1:3 plasmid to PEI ratio, Sigma). Three days after transfection ...
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bioRxiv - Immunology 2022Quote: ... HESC-T were decidualized by culturing cells with 0.5 mM 8-Bromo-cAMP (Sigma B5386), 1 μM medroxyprogesterone acetate (MPA ...
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bioRxiv - Plant Biology 2021Quote: ... 2 h) and incubated with primary antibody (anti-HA, 1:1000, Sigma) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... propyl acetate and 3-methyl-2-buten-1-ol (Sigma). Odorants were selected based on previous work using this task (Gu & Li ...
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bioRxiv - Cell Biology 2021Quote: ... 1/100 Phosphatase Inhibitor Cocktail 2 and 3 (Sigma Aldrich). Per condition ...