Labshake search
Citations for Millipore Sigma :
551 - 600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... Superovulation was performed by intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (PMSG, Sigma) followed by 5 IU human chorionic gonadotrophin (hCG ...
-
bioRxiv - Developmental Biology 2024Quote: ... incubated for 3 minutes in accutase (Sigma, A6964), and tapped to release the cells from the flask.
-
bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies (see Table S11) were incubated overnight at 4°C in 1% BSA (Sigma, A2153), then washed 3 times ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1 μM of the MEKi PD03 (PD0325901, Sigma PZ0162) and 1000 U/ml of LIF (prepared in house) ...
-
bioRxiv - Developmental Biology 2024Quote: ... 0.01% Tween20 (Sigma, P1379) in PBS/PVP ...
-
bioRxiv - Developmental Biology 2024Quote: ... and then the embryos were incubated in KSOM drops (Millipore, MR-101-D) covered in mineral oil (NidOil ...
-
bioRxiv - Developmental Biology 2024Quote: ... Blocking was performed for 1 hour in 2% Donkey Serum (Sigma, D9663), 0.1% BSA (Sigma ...
-
bioRxiv - Developmental Biology 2024Quote: ... mESCs were plated into N2B27 media supplemented with 2 µM IWP2 (Millipore, 681671), 1 µM of the MEKi PD03 (PD0325901 ...
-
bioRxiv - Developmental Biology 2024Quote: ... E2.5 embryos were flushed from the oviducts using M2 medium (Sigma, M7167), and E5.5-E6.5 embryos were dissected from the uterus ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1 µM of the MEKi PD03 (PD0325901, Sigma, PZ0162) and 1000 U/ml of LIF (prepared in house) ...
-
bioRxiv - Developmental Biology 2024Quote: For longer culture of E2.5 embryos the zona pellucida was removed by brief incubation in acidic Tyrode’s solution (Sigma, T1788) and then the embryos were incubated in KSOM drops (Millipore ...
-
Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... Doxycycline was added at 0.5 ug/ml (Millipore Sigma, D5207) in treatment wells ...
-
Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... mESCs were grown on tissue culture treated plates coated with 0.1% gelatin (Sigma G1890). For routine passaging ...
-
bioRxiv - Developmental Biology 2024Quote: ... the media was supplemented with either 250 nM 4OHT (4-Hydroxytamoxifen, Sigma, H7904) for ERK induction ...
-
bioRxiv - Developmental Biology 2024Quote: ... Embryos were permeabilized for 15 minutes in 0.25% Triton X-100 (Sigma, T8787) in PBS/PVP (3 mg/ml PVP (Polyvinylpyrrolidone ...
-
bioRxiv - Developmental Biology 2024Quote: ... CRAFR26 embryos were cultured in KSOM supplemented with 250 nM PD17 (PD173074, Sigma, P2499) for 24h ...
-
bioRxiv - Developmental Biology 2024Quote: ... the c-RAF kinase construct was induced by adding 250 nM 4OHT (4-Hydroxytamoxifen, Sigma, H7904) to the media.
-
bioRxiv - Developmental Biology 2024Quote: ... on Fibronectin-coated plates (Millipore, FC010), into N2B27 media supplemented with 12 ng/ml bFGF (Peprotech ...
-
bioRxiv - Cell Biology 2024Quote: ... anti-TAX1BP1 (Sigma, HPA02432), anti-SQSTM1 (Cell Signaling ...
-
bioRxiv - Cell Biology 2024Quote: ... stained with DAPI (Sigma-Aldrich), and mounted ...
-
Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... 250 ul of 1x PBS + 90% glycerol (Millipore Sigma, G2025) + 0.1% sodium azide was added to each well of the 8 well chamber slide ...
-
bioRxiv - Cell Biology 2024Quote: ... anti-vinculin (Cat#V9131, 1:30K), and anti-PMP70 (Cat#SAB4200181 for WB, 1:1000) antibodies were obtained from Sigma. Anti-p62 (Cat#sc-28359 ...
-
bioRxiv - Developmental Biology 2024Quote: Decamethonium bromide (DBr) (Sigma-Aldrich D1260) was diluted to 10 mg/ml in Hank’s Buffered Sterile Saline (HBSS ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were grown in Dulbecco’s Modified Eagle Medium low glucose (DMEM, Roth) supplemented with Ham’s Nutrient Mixture F12 (F12, Sigma) 10% Fetal Bovine Serum (FBS ...
-
“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Equal amounts of protein were electrophoresed on a 12% SDS– polyacrylamide gel and transferred to PVDF membranes (Millipore). Blots were blocked for 1 hour at RT (room temperature ...
-
bioRxiv - Developmental Biology 2024Quote: ... Two drops (5μl and 0.3μl) of premixed 3x Tribolium saline + Schneider’s solution (1:1) supplemented with 100 µmol/l of amaranth (Sigma-Aldrich, St Louis, MO, USA) were dropped under paraffin oil and their diameter measured ...
-
bioRxiv - Developmental Biology 2024Quote: ... animals were transferred to a 96-well plate in a container filled with silica gel beads (Sigma-Aldrich, MO, USA) to produce a low-humidity environment (approx ...
-
“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Total proteins extraction from hPSCs was performed in ice-cold RIPA protein extraction buffer (Sigma) supplemented with protease inhibitors (Protease inhibitor cocktail set I ...
-
“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... and then with Propidium Iodide (PI) (40 ⎧g/ml) (Sigma-Aldrich) for 5 minutes ...
-
bioRxiv - Developmental Biology 2024Quote: ... and 103 U/ml LIF (Millipore). To generate mouse spinal organoids ...
-
“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... and cell staining was carried out on cells fixed with 4% paraformaldehyde in PBS with 0.5% BSA and 0.5% Saponin (Sigma). Anti-OCT-4 (1:1000 ...
-
bioRxiv - Microbiology 2024Quote: ... plates were washed with water and stained with 200 µL of 0.1 % (w/v) crystal violet (Sigma-Aldrich, UK) and incubated at room temperature for 10 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... cells were diluted (1:10000) into new microtiter plates with fresh TSB supplemented with 1 % (w/v) glucose (Sigma-Aldrich, UK). The plates were sealed with a gas permeable membrane (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... A mixture of Protein A agarose (Millipore, 16-157) and Protein G agarose (Millipore ...
-
bioRxiv - Cancer Biology 2024Quote: ... NEUROG3 (Sigma, SHCLNG-NM_020999, TRCN0000427521) and empty vector TRC2 (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were treated with hygromycin (400 µg/mL) (Millipore # 400052) or puromycin (2µg/mL ...
-
bioRxiv - Cancer Biology 2024Quote: ... CoREST2 (Sigma, SHCLNG-NM_17585, TRCN0000122645), CoREST3 (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... or puromycin (2µg/mL) (Sigma-Aldrich #P8833).
-
bioRxiv - Cancer Biology 2024Quote: ... JAK2 (Sigma, SHCLNG-NM_004972, TRCN000002181), CoREST1 (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... CoREST2-FLAG overexpression (VectorBuilder) and knockdown of STAT3 (Sigma, SHCLNG-NM_003150, TRCN0000329887), JAK2 (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... and empty vector TRC2 (Sigma, #SHC201), the following lentiviral protocol was used ...
-
bioRxiv - Cancer Biology 2024Quote: ... CoREST1 (Sigma, SHCLNG-NM_015156, TRCN0000418894), CoREST2 (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... CoREST3 (Sigma, SHCLNG-NM_018254, TRCN0000116089), LSD1 (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... LSD1 (Sigma, SHCLNG-NM_015013, TRCN0000046071), NEUROG3 (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1x Sigma Phosphatase Inhibitor (Sigma, 5726), 2x Halt Phosphatase and Protease inhibitor Cocktail (Thermo Fisher ...
-
bioRxiv - Microbiology 2024Quote: ... Overnight cultures of the desired bacterial strains were prepared in microtiter plates using 200 µL of TSB supplemented with 1 % (w/v) glucose (Sigma-Aldrich, UK) to facilitate biofilm formation (Croes et al. ...
-
bioRxiv - Molecular Biology 2024Quote: ... The complex was purified using its incubation with 1 ml of anti-FLAG M2 affinity gel (Sigma) 6h at 4CC ...
-
bioRxiv - Molecular Biology 2024Quote: ... An equivalent of 1 μl of the original IVT was used for TCA precipitation and filtered onto a GF/C membrane (Millipore). The cap-analogue beads were washed 5 times with binding buffer and the final bead fraction suspended in SDS-PAGE sample buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... Target cells were transduced on two consecutive days by supplementing the lentiviral supernatant with 8 μg/mL polybrene (Sigma). One day after the final transduction ...
-
bioRxiv - Molecular Biology 2024Quote: ... Purified plasmid constructs were used to transform the bacterial expression strain Rosetta™(DE3)pLysS (EMD Millipore). To express protein ...