Labshake search
Citations for Millipore Sigma :
4251 - 4300 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2019Quote: ... Labeled standards for indole-3-acetic acid and N-(3-indolylacetyl)-DL-aspartic acid were obtained from Sigma-Aldrich. Calibration curves were linear (r values = >0.99 ...
-
bioRxiv - Neuroscience 2019Quote: Female fly heads (30-50 per N) at 2 DAE (days after eclosion) were homogenized using a mechanical pestle homogenizer in 200μl Trizol (Sigma). After adding 800μl Trizol with 200μl chloroform and a 15min centrifugation at 4°C ...
-
bioRxiv - Bioengineering 2019Quote: ... The glass micropipettes were surface-modified through silanization with N-(2-aminoethyl)- 3-aminopropyltrimethoxysilane (EDA) (Sigma Aldrich catalog # 440302) in order to reduce stiction forces between polysilicon and the wall of the miniaturized glass micropipette during their integration ...
-
bioRxiv - Biochemistry 2019Quote: ... Antibodies were diluted to 17.5 μg/ml in 100 μl 0.1M 2[N-Morpholino]ethanesulfonic acid (MES)-buffer (M2933, Sigma-Aldrich), pH 4.5 ...
-
bioRxiv - Cell Biology 2019Quote: ... inhibitors (AR-C155858 – Tocris #4960, LY294002 – Sigma #L9908, Quercetin – Sigma #00200595, Cycloheximide – Sigma #C1988 and N-acetylcysteine – Sigma #A7250) were applied 15 min before lactate or pyruvate stimulation ...
-
bioRxiv - Cell Biology 2019Quote: ... larvae were maintained in E3 containing 0.2 mM N-phenylthiourea beginning 1 day post fertilization (dpf) (PTU, Sigma Aldrich). All zebrafish lines used in this study are listed in Table S1.
-
bioRxiv - Biochemistry 2019Quote: ... The sample was incubated for one minute before blotting with filter paper Whatman n° 1 (Sigma Aldrich, Munich, Germany). Subsequently ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Marchantia 5-7 day old sporelings were co-cultivated with 100 µL of Agrobacterium culture in 4 mL of liquid 0.5x Gamborg B5 plus supplements (0.1% N-Z amino A (Sigma C7290), 0.03% L-glutamine ...
-
bioRxiv - Molecular Biology 2019Quote: ... male Wistar rats (n=5-6/group) were deeply anesthetized with sodium phenobarbital (Sigma-Aldrich; 50 mg/kg i.p.), and then positioned in a stereotaxic frame (TrentWells ...
-
bioRxiv - Cancer Biology 2020Quote: ... plates were washed 10 times with PBS/Tween and substrate was added (o-phenylenediamine dihydrochloride tablets) and then quenched with 0.2 N sulfuric acid (Sigma) after colour appears visible ...
-
bioRxiv - Cell Biology 2020Quote: ... and 2μL of purified gDNA was added to 5μL of the 2X REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich), 0.2μL 10μM forward/reverse primer mix ...
-
bioRxiv - Microbiology 2021Quote: ... Gold strain was performed in 800 ml 2xYT o/n at 37°C after induction with 0.5 mM IPTG (Sigma). Cells were harvested by centrifugation ...
-
bioRxiv - Bioengineering 2021Quote: ... the particles were transferred into aqueous solution (DPBS or cell media) containing 10µM N-ethylmaleimide (NEM, Sigma-Aldrich, E3876) at a pH of 6.5 ...
-
bioRxiv - Bioengineering 2021Quote: ... 2.5-month-old CON male and female (C57Bl/6J, n=7-9/sex/group) were injected with 0.5 mg/kg of raloxifene hydrochloride (SIGMA) for 6 weeks ...
-
bioRxiv - Bioengineering 2020Quote: ... O,O′-bis[2-(N-succinimidyl-succinylamino)ethyl]polyethylene glycol with molecular weight of 2kDa (NHS-PEG-NHS, Sigma) was mixed with the protein solution at 10 w/w% ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was then labeled with the MDCC (7-Diethylamino-3-[N-(2-maleimidoethyl) carbamoyl]coumarin) from Sigma-Aldrich. Unreacted MDCC was removed by ion exchange using a Bio-Rex70 column ...
-
bioRxiv - Neuroscience 2020Quote: ... cells were permeabilized for 20 min at RT with DPBS + 0.1% Triton X-100 (Sigma, Cat N° T8787-250ML) followed by blocking for 20 min at RT in 5% normal donkey serum (Sigma ...
-
bioRxiv - Cell Biology 2021Quote: ... then triturated and plated on poly-L-lysine coated 8-well glass chambers (Sigma, P2636, Cellvis, C8-1-N) at a density of 25,000 cells/well in 5 % horse serum (Gibco 26050-088 ...
-
bioRxiv - Neuroscience 2019Quote: ... or a control vehicle (n=11; 5% ethanol, 5% Cremphor EL [C5135-500G; Sigma-Aldrich, Inc.], and 90% saline) 30 minutes before testing ...
-
bioRxiv - Microbiology 2021Quote: ... was incubated with 200x molar excess of succinimidyl 4-[N-maleimidomethyl]cyclo-hexane-1-carboxylate (SMCC-Sigma-Aldrich #M5525) for 2.5 hours at room temperature to generate maleimide-DNA capable of sulfhydryl-reactive crosslinker chemistry with peptides with terminal cysteines ...
-
bioRxiv - Neuroscience 2020Quote: Sox14GFP/+ mouse brain samples (n=3) were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...
-
bioRxiv - Cell Biology 2021Quote: ... 100 μM hydrogen peroxide (H2O2) for 5 min or alkylated with 20 mM N-ethyl maleimide (NEM; Sigma #E3876) in 100 mM sodium phosphate ...
-
bioRxiv - Cell Biology 2021Quote: ... saturated 1 hr in 4% BSA and incubated O/N with rabbit anti-Laminin antibody (1:300, Sigma-Aldrich) to reveal myofiber outlines ...
-
bioRxiv - Cell Biology 2022Quote: Representative slices from different areas of the tissue were fixed O/N with 10% neutral buffered formalin (Sigma-Aldrich). Next ...
-
bioRxiv - Neuroscience 2020Quote: ... live TTX-treated neurons were incubated with either a mouse monoclonal anti-N-terminal GluA1 (1:40, Merk-Millipore) or GluA2 (1:30 ...
-
bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
-
bioRxiv - Biochemistry 2021Quote: ... The residual acetone was removed by brief evaporation and the precipitate was resuspended (600μl) in urea buffer containing 100mM N-ethylmaleimide (d0-NEM) (E3876, Sigma), dissolved in EtOH ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 30 min at 4°C with 22C11 antibody (anti-N-terminus of APP, Millipore) or anti-FLAG (Millipore) ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 30 min at 4°C with 22C11 antibody (anti-N-terminus of APP, Millipore) or anti-FLAG (Millipore) ...
-
bioRxiv - Neuroscience 2020Quote: ... Experimental mice of the hM4Di or control group were randomly treated with saline or Clozapine-N-oxide (CNO 3mg/kg i.p. in 0.9% saline, Sigma-Aldrich) and placed for 1 hour in their home cage where the test was subsequently carried out (Figure 2) ...
-
bioRxiv - Biochemistry 2021Quote: ... One milliliter of the N-oxide solution was added dropwise to 3 mL of sodium acetate (37.5 mM; pH 5.3; Sigma Aldrich), followed by incubation at room temperature for 24 hours ...
-
bioRxiv - Neuroscience 2022Quote: Wasp eggs were collected upon bleeding 50 infested larvae in PBS 1X added with some N-Phenylthiourea crystals (Sigma) to prevent haemocyte melanisation ...
-
bioRxiv - Genetics 2022Quote: ... The homogenates were then lysed on ice for 30 min in nucleus lysis buffer (HEPES 50 mM, EDTA 10 mM, N lauryl sarkosyl 0.5%, sodium butyrate 10 mM, EDTA free protease inhibitor cocktail Sigma). DNA was sheared using a Bioruptor pico from Diagenode for 10 cycles (30 sec on ...
-
bioRxiv - Immunology 2022Quote: ... cells using minimal essential medium supplemented with 0.2% bovine serum albumin and 2 μg/mL N-Tosyl-L-phenylalanin-chlormethylketon (Sigma). For the determination of the tissue culture infectious dose 50 (TCID50) ...
-
bioRxiv - Developmental Biology 2019Quote: ... Denuded fail to fertilize M-II oocytes (n=8) were obtained 36h post-ovulation and cultured briefly (2-4h) in 50µl KSOM medium (Millipore) containing 0.4% BSA at 37°C ...
-
bioRxiv - Physiology 2019Quote: ... general Cl− transport was inhibited with 4,4’-diisothiocyano-2,2’-stilbenedisulfonic acid (DIDS, 100 μmol L−1, N = 4) (all chemicals from Sigma Aldrich, St ...
-
bioRxiv - Microbiology 2019Quote: ... Samples were dried in a vacuum centrifuge and were derivatised by the addition of 0.1mL BSTFA (N,O-Bis(trimethylsilyl)trifluoroacetamide) TMCS (Trimethylchlorosilane) (99:1, Sigma) and 0.3 mL anhydrous pyridine (Sigma ...
-
bioRxiv - Neuroscience 2019Quote: DNA from offspring was extracted from mouse ear punches using Extract-N-Amp® kit (Sigma, St. Louis, MO) and genotyped using a multiplex PCR reaction ...
-
bioRxiv - Zoology 2020Quote: PCR amplification of two genomic regions were performed using 12.5 μl of the Extract-N-AmpTM Tissue PCR kit (Sigma), 1 μl of each primer ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... IVM was converted into a fluorescent molecule using N-methylimidazole and trifluoroacetic anhydride (Sigma Chemical, St Louis, MO, USA). An aliquot (100 μl ...
-
bioRxiv - Molecular Biology 2020Quote: ... The pellets were resuspended in 200 µL of W-Buffer and treated with 1% SDS (Sigma cat. n° 05030) and 0.1 mg Proteinase K (Euroclone ...
-
bioRxiv - Molecular Biology 2020Quote: ... The aqueous phase was filtered with Millipore ultrafree MC tubes and then precipitated with isopropanol (Sigma, cat. n° I9516) at - 80°C for 2 hours or overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... and lysed in 300 μl of lysis buffer (20mM Tris pH=8.0, 150mM NaCl, 1% Triton) containing 100 μM of N-Ethylmaleimide (Millipore-Sigma) and 1Xprotease inhibitor cocktail (Biotool ...
-
bioRxiv - Developmental Biology 2020Quote: ... pigmentation of embryos was prevented by treating them with 0.002% N-phenylthiourea (PTU) (Sigma-Aldrich, St. Louis, US-MO) starting at 25hpf ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were washed once with cold PBS containing 20 mmol/L N-ethylmaleimide (NEM, Cat. No. E3876, Sigma-Aldrich) and then lysed in radioimmunoprecipitation assay buffer (25 mmol/L Tris (Cat ...
-
bioRxiv - Cell Biology 2020Quote: ... Group 2 (DN+CL, n=8): mice received surgical denervation of BAT and intraperitoneal injections of CL316243 (C5976; Sigma) at 1mg/kg for consecutive 14 days ...
-
bioRxiv - Cell Biology 2020Quote: ... 10 % glycerol and bromophenol blue) containing 50 μM phenylmethylsulfonyl fluoride (PMSF) and 50 μM N-ethylmaleimide (all Sigma-Aldrich). For LMα3 ECM processing blots ...
-
bioRxiv - Microbiology 2021Quote: ... dissolved in PBS or 0.8 mg/ml fMLP 2.5 ml (N-Formyl-Met-Leu-Phe, F3506, Lot#MKCC5355; Sigma-Aldrich), where 2 mg fMLP was dissolved in 50 μl DMSO and then in 2.5 ml PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... Clozapine-N-oxide (NIDA Drug Supply Programme) was dissolved first in 5% DMSO (Dimethyl Sulfoxide, Sigma-Aldrich, Poole, UK) and then in sterile 0.9% NaCl (Henry Schein Ltd ...
-
bioRxiv - Plant Biology 2020Quote: The ability of pollen to remove OH• (generated in a Fenton reaction) was analyzed in experiments with spin trap DMPO (5,5-dimethyl-1-pylloline-N-oxide) (Sigma). First H2O2 and spin trap were added to a suspension of pollen ...