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Citations for Millipore Sigma :
4351 - 4383 of 4383 citations for MOA SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: The PLA assay was performed to determine the in situ binding between LC3 and ATG9a or ATG9b by using the Duolink® In Situ Red Starter Kit purchased from Sigma Aldrich (catalog number DUO92101), following the protocol provided by the manufacturer.
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bioRxiv - Genomics 2020Quote: ... Supernatant was aspirated before the isolation of single nuclei was performed using the Nuclei PURE Prep nuclei isolation kit (Sigma-Aldrich, St. Louis, MO, USA) in conjunction with a modified protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: Total RNA of the collected/dissected petals (see supplementary fig. S2) was extracted using Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). cDNA was synthesized following the methods described in Supporting Information Methods S4 in Lin and Rausher (2021) ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was collected avoiding to disturb the pellet and was mixed with 750 microliters of Binding buffer of Total Spectrum RNA kit (Sigma–Aldrich, Saint Louis, MO, USA). Subsequently the extraction proceeded following manufacturer instructions.
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bioRxiv - Microbiology 2021Quote: RNA extract from a single female adult tick was converted to cDNA using the Complete Whole Transcriptome Amplification Kit (WTA2, Sigma-Aldrich, Saint Louis, MO, USA). Purification of cDNA was done using the MSB Spin PCRapace kit (Stratec Molecular ...
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bioRxiv - Microbiology 2020Quote: ... All transformants were screened by PCR using gene-specific primers (Table S2, Fig. S2c) and were verified by Southern hybridization analyses (DIG DNA Labeling and Detection Kit, Sigma-Aldrich, St. Louis, MO, USA) (Fig ...
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bioRxiv - Immunology 2021Quote: ... Vero E6 monolayers are washed and incubated for 5-6 days at 37 degrees after which plates are scored using the vitality marker WST-8 (colorimetric cell counting kit, Sigma Aldrich, cat 96992-3000TESTS-F). To this end ...
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bioRxiv - Bioengineering 2020Quote: Plasmid DNA was purified after overnight culture in standard lysogeny broth containing 100 µg/mL ampicillin using a miniprep plasmid DNA isolation kit (Sigma Aldrich, St Louis, MO; PLN350) and quantified using spectrophotometry (NanoDrop One ...
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bioRxiv - Cancer Biology 2022Quote: ... equal number of WT BBS and BBS Mut MCF7 and HCC1806 cells were lysed using ice-cold lactate assay buffer provided in the Lactate assay kit (Sigma, MAK064-1, lot no. 3F09K06270). The lactate quantification was performed using the deproteinized lysates by following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from 21 days-after-flowering (DAF) sunflower seeds using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: Tissues were ground into a fine powder in liquid nitrogen and total RNA was extracted using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO). Sequencing libraries were produced using the TruSeq RNA Sample Preparation kit v2 (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mg of leaf powder was used for RNA extraction following protocol A of the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO). RNA was eluted in 50 μL elution buffer ...
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bioRxiv - Plant Biology 2022Quote: Harvested tissue was grounded to a fine powder in liquid nitrogen and RNA was extracted using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 100 mg of frozen and ground samples using the Spectrum™ Plant Total RNA Kit (Sigma, St. Louis, Missouri, USA) according to the manufacturer’s Protocol A ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from frozen samples in the manual of the GenElute Bacterial DNA Isolation kit (Sigma Aldrich, NA2110-1KT, St. Louis, MO, USA) with the following minor modifications as previously described60 ...
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bioRxiv - Genetics 2024Quote: RNA was extracted from 100 mg of frozen ground leaves per replicate using the Spectrum Plant Total RNA kit (Sigma-Aldrich, St Louis, MO, USA) followed by treatment with RNase-free DNase using the On-Column DNase I Digestion Set (Sigma-Aldrich) ...
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bioRxiv - Genetics 2024Quote: ... quantification in the heads of both starved and non-starved miR-210 KO and WT flies was carried out using the Triglyceride Quantification Kit (Sigma-Aldrich, St. Louis, Missouri, USA), following the provided manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were isolated from snap-frozen frontal cortex (anterior to the anterior forceps of the corpus callosum) samples from one hemisphere using Nuclei Isolation Kit: Nuclei EZ Prep (Sigma-Aldrich Cat. No. NUC-101). Briefly ...
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bioRxiv - Plant Biology 2023Quote: The total RNA was extracted from PEG-treated (24 h) and untreated control (0 h) samples using Spectrum plant total RNA Kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from a tail biopsy and genotypes were determined using the Extract-N-Amp Tissue PCR kit (Sigma-Aldrich, St Louis, MO, USA). The following primer sequences (Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... the first step was to remove the iodixanol through dialysis using Pur-A-LyzerTM Mini 6000 dialysis kit (PURN60030, Sigma-Aldrich, Co., STL, MO, USA). Then SEC-DGUC-1 was subjected to in-solution digestion prior to fractionation on an Xbridge™ C18 column (4.6 × 250 mm ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we grinded the plant tissue into a fine powder with a pestle and a mortar in the presence of liquid nitrogen and processed the samples using the Spectrum™ Plant Total RNA-Kit from Sigma-Aldrich (product number: STRN250), including on-column DNA digestion using the on-column DNAse kit (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... To detect Edu signal was used “EdU IV Imaging Kit 647 S” based on the Click-it reaction with azides conjugated to fluorochromes (Sigma Aldrich, BCK647-IV-IM-S) following instructions of the manufacturer.
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bioRxiv - Immunology 2021Quote: Cytotoxicity was assessed via lactate dehydrogenase (LDH) concentrations measured in 100 ul of basolateral medium incubated with the reaction mixture of a cytotoxicity detection kit (Sigma-Aldrich, Roche, Saint Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from leaf tissue collected at each time point for all species using the Spectrum Plant Total RNA Kit (Sigma-Aldrich Co., St. Louis, MO, USA) following Protocol A ...
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bioRxiv - Neuroscience 2022Quote: ... and TNF-α were measured simultaneously using the multiplexed kit “Mouse High Sensitivity T Cell Magnetic Bead Panel” (MHSTCMAG-70K, MILLIPLEX, EMD Millipore Corp., Billerica, MA, USA). The assay was performed according to the manufacturer’s instructions with an overnight incubation step (17 hours ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... three samples consisting of both treated and mock seedlings were collected and used for total RNA extraction with the Sigma mirPremier® microRNA Isolation Kit (Sigma Aldrich, St. Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kit FITC (Cat. No. 88-8005-72) was used in combination with Propidium Iodide Solution (Sigma-Aldrich, Cat. No. P-4864-10mL) as described in manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in matched BAL fluid and plasma collected from patients were measured using the multiplexed human cytokine/chemokine magnetic bead kits from Millipore (HCYTMAG-60K-PX41 and HCYP2MAG-62K) according to manufacturer’s protocol (HCYTOMAG-60K Rev ...
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bioRxiv - Microbiology 2024Quote: ... A 1.5 mL of overnight culture was used for DNA extraction using the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich, St. Louis, MI, United States) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... pH 8.0 to a final concentration of 0.2nM and PCR amplified for cloning into T7 phage vector arms (Novagen/EMD Millipore Inc. T7 Select 10-3 Cloning kit). Detailed protocol can be found in dx.doi.org/10.17504/protocols.io.j8nlkkrr5l5r/v1 ...
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bioRxiv - Cell Biology 2023Quote: ... ∼1 kb 5’ homology arm and ∼1 kb 3’ homology arm of msps genomic region were amplified from the genomic DNA (Sigma, Extract-N-Amp™ Tissue PCR Kit), mutated to be insensitive to gRNAs ...
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bioRxiv - Molecular Biology 2024Quote: ... The presence/absence of receptor–receptor molecular interactions in the samples was detected using the Duolink II in Situ PLA Detection Kit (developed by Olink Bioscience, Uppsala, Sweden; and now distributed by Sigma-Aldrich as Duolink®, using PLA® Technology).