Labshake search
Citations for Millipore Sigma :
4051 - 4100 of 4383 citations for MOA SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... In situ hybridization was completed according to a standard protocol (Cunningham and Monk, 2018) using DIG RNA labeling kit T7/SP6 (Millipore Sigma #11175025910). RNA probes were cleaned using SigmaSpin Sequencing Reaction Clean-UP (Millipore Sigma #S5059-70EA) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Perifusate fractions were collected at the flow rate of 3ml/min and insulin was measured using radioimmunoassay kit (Millipore, Cat #: RI-13K). Insulin secretion were normalized to IEQ.
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bioRxiv - Biochemistry 2022Quote: ... The Duolink procedure was conducted according to the manufacturer’s instructions with the Duolink® In Situ Red Starter Kit Mouse/Rabbit (Duolink®, Sigma□Aldrich), with secondary antibodies contained in the kit ...
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bioRxiv - Cancer Biology 2022Quote: ... and carbonic anhydrase (29 kD) from a combination of two kits (GE Healthcare, catalogue No. 28-4038-41, Chicago, Illinois, IL, USA and MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Immunology 2022Quote: ... Cultures were stimulated with 20 ng/ml PMA for 3 hours and assessed with a mouse IFN-γ ELISA kit (Sigma-Millipore). For airway epithelial cell culture ...
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bioRxiv - Microbiology 2022Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). All chemicals and antibiotics were obtained from Sigma unless otherwise noted.
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: The determination of telomerase enzyme activity was done in HEKn cells by using TeloTAGGG Telomerase PCR Plus Kit (Sigma Aldrich, USA, 12013789001) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... The genomic DNA of kanamycin-sensitive and chloramphenicol-sensitive clones was isolated using the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich), and analyzed by PCR to confirm the gene replacement using primers pairs TP_ctg3-43_f / TP_ctg3-43_r in reactions (20 μL ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 200 mg of pollen tubes using SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). RNA-seq libraries were performed using the non-stranded mRNA Kit from Illumina (San Diego ...
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bioRxiv - Microbiology 2021Quote: ... DNase I treatment was given to avoid genomic DNA contamination followed by purification of total RNA using GenElute™ Total RNA Purification Kit (Sigma-Aldrich). The purified RNA was quantified using a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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bioRxiv - Neuroscience 2021Quote: ... were measured with a human cytokine/chemokine panel utilizing Luminex 200 xMap technology and the MILLIPLEX MAP ® multiplex kit (Luminex xMAP technology; EMD Millipore, Chicago ...
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bioRxiv - Neuroscience 2020Quote: ... we spun down for 3 min at 13.000 rpm at 4° C and performed glucose assays on the supernatant using a Glucose (GO) assay kit from Sigma (GAGO-20).
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bioRxiv - Immunology 2021Quote: ... Concentrations were determined in duplicate by fluorescent-coded magnetic beads (MagPlex-C; MILLIPLEX. MAP Kit, Canine Cytokine Magnetic Bead Panel, 96-Well Plate Assay, CCYTO-90K, Millipore, Billerica, MA), based on the Luminex xMAP technology (Luminex 200 ...
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bioRxiv - Biophysics 2021Quote: The ability of SHP2 to dephosphorylate the phosphopeptides was evaluated through a malachite green phosphatase assay (PTP assay kit 1 Millipore, MA, USA). The BTAM peptide and the following monophosphorylated peptides derived from known SHP2 substrates were used for comparison ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three more times in TBS-T before antibody detection using enhanced chemiluminescence horseradish peroxidase substrate detection kit (Millipore, Hertfordshire, UK). Imaging was conducted using a G:Box Chemi-XR5 (Syngene ...
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bioRxiv - Developmental Biology 2022Quote: ... Terminal deoxynucleotide transferase (TdT)-mediated dUTP nick end labeling (TUNEL) was performed on frozen retinal cryosections using the ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit (Millipore, Billerica, MA). TUNEL staining was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: The number of apoptotic cells were determined using the In Situ Cell Death Detection Kit (Sigma-Aldrich, 11684795910, St. Louis, MO, USA) in paraffin-embedded tissue sections ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were obtained from ATCC and were tested negative for mycoplasma using LookOut® PCR-based kit (MP0035-1KT Sigma-Aldrich). HEK293-Klotho cells stably expressing Klotho were a gift from Dr ...
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bioRxiv - Plant Biology 2019Quote: RNA was extracted from leaves and roots of sunflowers from the genotype HA412-HO using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich). Single strand copy DNA (ss-cDNA ...
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bioRxiv - Pathology 2020Quote: ... Eleven markers were simultaneously measured in the samples using a MILLIPLEX Mouse Cytokine/Chemokine 11-plex kit (Millipore, St. Charles, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2020Quote: ... Cells were allowed to adhere overnight at 26°C prior to being assessed for β-galactosidase activity associated with cellular senescence using a commercially available Senescence Cells Histochemical Staining Kit (Sigma Aldrich). The manufacturer’s protocol was followed with the following modifications ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from immature seeds of Trombay wild and TU94-2 plants with the help of Spectrum Plant Total RNA Kit (Sigma-Aldrich, USA) and treated with DNAse-I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... in serum was analyzed by measuring 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc) using Ketone body assay kit (Sigma, No. MAK134) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... 300 μl of CHAMP buffer containing 4 nM Alexa488-conjugated anti-Flag antibody (Alexa Fluor 488 antibody labeling kit, Thermo Fisher; monoclonal BioM2, Sigma-Aldrich) was injected to wash off unbound RNP and label DNA-bound RNP complex ...
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bioRxiv - Systems Biology 2020Quote: Plasma aliquots of blood collected from the tail vein after 4-hour fasting were used to measure the concentration of glucose and triglycerides by enzymatic colorimetric assays using commercially available kits (Sigma-Aldrich, USA) according to manufacturer’s instruction [17] ...
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bioRxiv - Neuroscience 2021Quote: ... PDMS with the standard 1:10 weight ratio of crosslinker to base (SYLGARD 184 Silicone Elastomer Kit, Dow Chemical) was diluted with hexane (Sigma-Aldrich, 34859) in a volume ratio of 1:9 ...
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bioRxiv - Microbiology 2021Quote: ... USA) QIAamp 96 DNA QIAcube HT Kit with the following modifications: enzymatic digestion with 50 μg lysozyme (Sigma, St. Louis, MO, USA) and 5U each of lysostaphin and mutanolysin (Sigma ...
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bioRxiv - Physiology 2020Quote: ... Secretion of glycerol and non-esterified fatty acid from cultured adipocytes into HBSS was determined with assay kits from Sigma-Aldrich (FG0100) and FUJIFILM Wako Diagnostics-NEFA Reagent (NEFA-HR(2)) ...
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bioRxiv - Pathology 2021Quote: ... Cytokine and chemokine levels in monkey sera were measured using a MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex - Immunology (Milliplex MAP kit, Merck Millipore, Burlington, MA), which includes the following 23 cytokines and chemokines ...
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bioRxiv - Microbiology 2019Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). For E ...
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bioRxiv - Cell Biology 2019Quote: PLA was performed according to the manufacturer’s instructions using the Duolink In Situ Orange Kit Goat/Rabbit (Sigma Aldrich, Cat. No. DUO92106). Immunofluorescence was performed as previously described (Arno et al. ...
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bioRxiv - Biochemistry 2019Quote: ... β-hydroxybutyrate (HBA) was determined by coupled spectrophotomet-ric assay with β-hydroxybutyrate dehydrogenase (obtained as kit from Sigma, St. Louis, USA). It was verified that urine does not interfere with this kit ...
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bioRxiv - Microbiology 2019Quote: ... The a-P28 antibody was conjugated with the Cy3 fluorescent dye using the Cy®3 Ab Kit GE Healthcare (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: The intracellular ATP content in the HGs was measured with an Adenosine-5’-triphosphate bioluminescent assay kit (FL-AA, Sigma Aldrich Inc.). Bioluminescent luciferase-luciferin reactions provide the basis of simple ...
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bioRxiv - Genomics 2021Quote: ChIP was performed thrice independently for each antibody using 1×106 SK-N-SH cells for each transcription factor using the EZ-Magna ChIP kit (Millipore Sigma, USA), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Ethanol 70% (vol/vol) was added and the contents were placed in columns (GenElute® mammalian total RNA miniprep kit, Sigma Aldrich). The RNA was extracted according to the manufacturer recommendations ...
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bioRxiv - Physiology 2021Quote: The G3P concentration was quantified using the Glycerol-3-Phosphate Assay Kit (MAK207) according to the technical bulletin (Sigma-Aldrich, United States). The concentration of G3P was expressed in ng/μg protein.
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bioRxiv - Physiology 2020Quote: ... TUNEL staining was performed using a protocol modified from the ApopTag peroxidase in situ apoptosis detection kit (Merck-Millipore, Cat no. S7100). Paraffin sections of adipose tissues were prepared as above ...
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bioRxiv - Physiology 2021Quote: ... A 30-μL portion of supernatant was used to measure circulating glucose levels with glucose oxidase assay kit (Sigma-Aldrich, GAGO-20) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Aforementioned single clone cultures were STR validated and screened for any mycoplasma contamination using PCR based Myco detection kit (Sigma, MP0035-1KT) before use ...
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bioRxiv - Cancer Biology 2021Quote: ATP levels were measured in control and constricted breast cancer cells (3 x 105 cells) using the ATP fluorometric assay kit (Sigma-Aldrich, MAK190), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Reduced and oxidized fractions of nicotinamide adenine dinucleotide phosphates (NADPH/NADP) were measured using the NADP/NADPH Quantitation kit (Sigma-Aldrich, USA) adhering to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IL-17A and TNFα were quantified in culture supernatants using the mouse-specific Milliplex® multi-analyte panel kit MT17MAG-47K (Millipore; Sigma) and the MagPix® instrument platform with related xPONENT® software (Luminex Corporation) ...
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bioRxiv - Cell Biology 2021Quote: DNA extraction from mouse tail clips obtained at 7-10 days of age was performed using REDExtract-N-Amp™ Tissue PCR Kit (Cat#R4775, Sigma Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Fluorescent membrane labelling of the VE was achieved using the CellVue Claret Far Red Fluorescent Cell Linker Kit (Sigma-Aldrich, MINICLARET-1KT). Briefly ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined using a Bradford protein assay kit (Thermo-Fisher, MA, USA) with bovine serum albumin (Sigma-Aldrich, MO, USA) as the standard ...