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Citations for Millipore Sigma :
4001 - 4050 of 4383 citations for MOA SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: Terminal deoxynucleotide transferase (TdT)-mediated dUTP nick end labeling (TUNEL) was performed on frozen retinal cryosections using the ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit (Millipore, Billerica, MA). TUNEL staining was performed according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was extracted from each three biological replicates of 100 mg pollen grains/pollen tubes by using a Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22, CRP; Millipore Sigma - RAB0096-1KT). In brief ...
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bioRxiv - Cancer Biology 2022Quote: For RNA-binding protein immunoprecipitation (RIP) ~3×107 A549 cells for each sample were processed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was used to assay for ANP levels using a competitive enzyme Immunoassay kit from Sigma-Aldrich (Catalog Number RAB0385). The levels of ANP were normalized for the amount of protein ...
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bioRxiv - Molecular Biology 2020Quote: The ATPase activity assay was performed in 96-well microtitre plates by using an ATPase/GTPase activity assay kit (Sigma-Aldrich, USA) according to the manufacturer’s instructions ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... plants grown under a long day photoperiod (16 h light/8 h dark, 60% humidity) using the SpectrumTM Total RNA extraction kit (Sigma-Aldrich, USA). The sample was enriched for mRNA using the Poly(A ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... Colonies were picked and grown in 5 ml liquid SD-L-W medium and the prey vectors were isolated using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich, USA) after cell wall disruption ...
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bioRxiv - Cell Biology 2022Quote: The in-situ interaction of NICD and active β-catenin was determined using the Duolink™ II secondary antibodies and detection kits (Sigma–Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Colorimetric assay to measure alanine aminotransferase activity in the cell lines was performed using the ALT activity assay kit from Sigma (MAK052-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cloning of the antigen library into the T7 phages was performed following manufacturer’s instructions (T7Select 10-3 cloning kit, catalog no. 70550-3, Merck-Millipore, Burlington, MA, USA).
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bioRxiv - Genomics 2020Quote: ... Chromatin was prepared using the MAGnify Low Cell ChIP-Seq Kit (Thermo Fischer) and ChIP was done for H3K4me3 (Millipore, # 07-473), H3K27ac (Abcam ...
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bioRxiv - Neuroscience 2019Quote: Individual wells from cultured cells and tissue from mice was collected and then the homogenate was fractionated utilizing a NXTRACT CelLytic™ NuCLEAR™ Extraction Kit (Sigma) according to the manufacturer’s instructions ...
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Multiple steps mediate ventricular layer attrition to form the adult mouse spinal cord central canalbioRxiv - Developmental Biology 2019Quote: ... TUNEL assay was performed on 20 μm thick cryosections using ApopTag® Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore, Cat. # S7100) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed for 1 min in 4% formaldehyde and stained for AP activity using the Alkaline Phosphatase Detection Kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2020Quote: DIG-labeled riboprobes were generated using 2ul of PCR template with the Roche DIG RNA Labeling Kit (T7) (Sigma aldrich, SKU 11277073910)
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bioRxiv - Microbiology 2019Quote: ... Positive bacteria clones were inoculated into 50ml LB/carbnecillin (100 μg/ml) culture for amplification and midiprep plasmid purification using GenElute™ HP Plasmid Midiprep Kit (NA0200, Millipore Sigma). All plasmids except T3D-L1 were amplified at 37°C for 14-16hrs ...
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bioRxiv - Pathology 2019Quote: ... Sections were stained for tartrate-resistant acid phosphatase (TRAP) to examine osteoclast activity and numbers (Sigma-Aldrich Kit, St. Louis, MO, USA), and Fast Green counterstaining and examined with a Nikon Eclipse 90i Advanced Research Scope ...
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bioRxiv - Biochemistry 2019Quote: The detection of apoptotic cells was based on the nick-end (TUNEL) labelling method using the ApopTag® in situ detection kit (S7100, Merck Millipore, USA), according to the manufacturing protocol.
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bioRxiv - Genetics 2019Quote: Quantitative amplification was performed according to manufacturer’s specifications using KAPA SYBR® FAST qPCR Kit Master Mix (2X) Universal (Sigma, cat. #07959397001). 12 separate RNA isolations were analyzed ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... R&D Systems cytokine kits (Minneapolis, MN) or a high throughput multiplex cytokine assay system (Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel, Millipore, MO, USA) was used for cytokine quantification ...
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bioRxiv - Cancer Biology 2019Quote: ... TUNEL staining was performed on deparaffinized sections to detect late-stage apoptotic cells using the ApopTag peroxidase in situ apoptosis detection kit (Millipore, Burlington, MA), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed using an ApopTag® In Situ Peroxidase Detection Kit (S7100, Merck Millipore, USA) according to the manufacturer’s recommendations and as previously described (36) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with a 1:3 molar ratio of heavy chain and light chain expression vectors using the Expi293 Transfection kit (Sigma-Aldrich; L3287) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated for 2 hours by the Autophagy Reagent A (rapamycin containing) from the FlowCellect™ Autophagy LC3 Antibody-based Assay Kit (Millipore, FCCH100171), which have been diluted at 1:50 as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were lysed and the ATP content was measured by an ATP assay kit per manufacturer’s instruction (Millipore Sigma, n = 3).
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bioRxiv - Bioengineering 2020Quote: ... The alkaline phosphatase (AP) staining was performed 72 hours after seeding using the Leukocyte Alkaline Phosphatase kit (Sigma, St. Louis, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed a further three times in TBS-T prior to antibody detection using enhanced chemiluminescence horseradish peroxidase substrate detection kit (Millipore, Hertfordshire, UK). Imaging was undertaken using a G:BOX Chemi-XR5 (Syngene ...
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bioRxiv - Cell Biology 2021Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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bioRxiv - Cancer Biology 2020Quote: ... and processed on ABI 3500Xl Genetic Analyzer and mycoplasma testing was conducted with the Venor™ GeM Mycoplasma Detection Kit (Cat# MP0025-1kt, Sigma-Aldrich) both were done as recent as 1/9/2020 ...
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bioRxiv - Cell Biology 2021Quote: ... No contamination of mycoplasma was tested in every batch using the VenorGeM Mycoplasma Detection Kit (SIGMA-Aldrich MP-0025, St.Louis MO, USA), following the procedure indicated by the provider ...
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bioRxiv - Bioengineering 2021Quote: ... after 48 hours of incubation at 37°C were performed according to the manufacturer’s instruction (Malachite Green Phosphate Assay Kit, Sigma-Aldrich, The Netherlands). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Plant Biology 2020Quote: ... Up to 100 mg of liquid nitrogen ground tissue was applied to the SpectrumTM Plant Total RNA kit (Sigma-Aldrich, Taufkirchen, Germany) according to the manufacturer’s instructions for protocol B ...
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bioRxiv - Plant Biology 2020Quote: ... and total RNA was extracted using a method combining CTAB lysis buffer and the Spectrum Total Plant RNA extraction kit (Sigma, Darmstadt, Germany) as described previously (83) ...
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bioRxiv - Microbiology 2021Quote: ... USA) QIAamp 96 DNA QIAcube HT Kit with the following modifications: enzymatic digestion with 50μg of lysozyme (Sigma, St. Louis, MO, USA) and 5U each of lysostaphin and mutanolysin (Sigma ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from Legionella strains cultured for 18 h in AYE medium using the GenElute™ Bacterial Genomic DNA Kit (NA2100, Sigma-Aldrich) and used as template for PCR reactions ...
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bioRxiv - Microbiology 2021Quote: ... Double-stranded cDNA was synthesized and amplified using a TransPlex whole-transcriptome amplification kit (WTA-2; Sigma-Aldrich, St. Louis, MO, USA) and antibody-inactivated hot-start Taq DNA polymerase (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... Sulfate was determined via the barium precipitation method using the Sulfate Assay Kit following manufacturer’s instructions (Sigma Aldrich, Saint Louis, MI, USA). Sample measurements were carried out in technical triplicates.
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bioRxiv - Physiology 2021Quote: The NEFAS concentration was quantified using the Free Fatty Acid Quantitation Kit (MAK044) according to the technical bulletin (Sigma-Aldrich, United States). The results are expressed in ng/μl.
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was isolated from 27 samples (‘EMEK3’, R bulk and S bulk × 3 treatments/ sampling time × 3 replicates) using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Detection of an interaction between BRCA1 and the cavin or CAV1 proteins was assessed using the Duolink™ II Detection Kit (Sigma Aldrich) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: Ect were labeled with PKH26 dye (PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Sigma Aldrich, cat. No. PKH26GL) as previously described [35] ...
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bioRxiv - Neuroscience 2020Quote: ... and COs derived from H9 hESCs and iPSCs were measured using the Human Oxidative Phosphorylation Magnetic Bead Panel Milliplex MAP Kit (Millipore–H0XPSMAG-16K), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... organelle and nuclear fractions were harvested every 12 h for 2 days using the Nuclei EZ Prep nuclei isolation kit (Sigma NUC-101) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... for amplifying the whole genome of each lysed tissue sample by the GenomePlex Single Cell Whole Genome Amplification Kit (WGA4-50RXN, Sigma-Aldrich, USA). For each sample ...
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bioRxiv - Cancer Biology 2021Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using the ApopTag® Peroxidase in situ Apoptosis Detection Kit (EMD Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Golgi apparatus was isolated from mouse liver or cultured HCs by sequential sucrose gradients using a Golgi Isolation Kit (GL0010-1KT, Sigma-Aldrich, USA), based on the protocol we previously established (Tang and Wang ...
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bioRxiv - Microbiology 2021Quote: ... Following further dehydration in a graded acetone series the agarose blocs were embedded in Spurr’s resin (Spurr’s Low Viscosity embedding kit, Sigma Aldrich, St Louis, USA) using acetone as intermedium ...
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bioRxiv - Cancer Biology 2021Quote: RIP assays were conducted to explore the relationship between lncRNA NEAT1 and miR-1299 via the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA). Initially ...