Labshake search
Citations for Millipore Sigma :
4201 - 4250 of 4383 citations for MOA SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Serial sections for trabecular bone were obtained from the distal femur at a thickness of 5 µm followed by tartrate-resistant acid phosphatase (TRAP) staining or alkaline phosphatase (ALP) staining using TRAP kits (Fast Red TR/Naphthol AS-MX, Sigma, St. Louis, MO) and NBT/BCIP (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: ... human bronchoalveloar lavage fluid and mouse serum of animals dosed with human THP were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0751) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... donor cells were labelled with CellVue Claret Far Red Fluorescent Cell Linker Mini Kit for General Membrane Labelling (Sigma Aldrich, Cat#MINCLARET-1KT) following manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Both cell lines were maintained in a 5% CO2 humidified incubator at 37°C and monitored periodically for mycoplasma contamination (Sigma Venor GeM Kit). Unless otherwise indicated ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was isolated using the Gene Elute Mammalian Total RNA Miniprep Kit according to the manufacturer’s protocol (Sigma–Aldrich, St. Louis, MO, USA). Concentration of the isolated RNA was determined photometrically with a BioSpectrometer® (Eppendorf ...
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bioRxiv - Biochemistry 2022Quote: ... were authenticated by short tandem repeat (STR) profiling and tested for mycoplasma contamination prior to experiments using the LookOut® Mycoplasma PCR Detection Kit (Sigma, MP0035-1KT). HEK293T (ATCC CRL-3216 ...
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bioRxiv - Immunology 2020Quote: ... GM-CSF and TNF-α using the Luminex kit according to the manufacturer’s instructions (Milliplex Multiplex Assays with Luminex, Millipore Sigma, Danvers, MA). Samples were assayed using the Luminex assay system (Magpix).
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bioRxiv - Molecular Biology 2020Quote: ... Densitometry was performed as previous described.19 Proximity ligations between ephrinb2/EphB4 in flow-aligned cells were conducted by using Duolink® In situ Red Starter Kit Mouse/Rabbit (Sigma Aldrich, MO). Numbers of individual ligations in raw and post-processed images were compared to test the validity of the quantification ...
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bioRxiv - Cancer Biology 2021Quote: ... glucose metabolic inhibitor 2DG (10 mM or 20 mM) was added 15 min before cell lysis and ATP determination using the ATP Bioluminescence Assay Kit HS II (Sigma catalogue number 11699709001) and analyzed on an Infinite M200 PRO Luminometer (Tecan).
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bioRxiv - Molecular Biology 2022Quote: Cell were fixed/permeabilized with BD Cytofix/Cytoperm™ Kit (Cat 554714) and stained with DAPI (4,6, diamidino-2-phenylindole, Sigma-Aldrich, Cat D9542). DAPI (1 in 100 dilution ...
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bioRxiv - Microbiology 2022Quote: ... the entire surface of the agar plate was scraped using a loop and deposited into 180 μl of lysis solution provided by the GenElute Bacterial Genomic DNA extraction kit (Sigma-Aldrich, Darmstadt, Germany). For approach 2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These products were purified by running on a 1% agarose gel and gel extracted using the GenElute™Gel Extraction kit (Sigma-Aldrich, USA). The extracted bands were Sanger sequenced by Eurofins (USA ...
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bioRxiv - Plant Biology 2022Quote: ... Full-length FIN4 mRNA in PCRII-TOPO vector was used to synthesize sense and anti-sense probes using a DIG-labeled probe synthesizing kit (Millipore-Sigma, catalog number 11175025910) with T7 RNA polymerase for sense probe and SP6 RNA polymerase for antisense probe synthesis with 1μg plasmid as template ...
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bioRxiv - Microbiology 2019Quote: ... Each viral DNA sample was then amplified using GenomePlex® Complete Whole Genome Amplification (WGA2) Kit from Sigma-Aldrich (Gudenkauf and Hewson, 2016). Two blank controls were included in this step ...
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bioRxiv - Immunology 2019Quote: Chitinase activity was analyzed by using the substrate and standard solutions of the fluorimetric chitinase assay kit (Sigma-Aldrich Chemie GmbH, Munich, Germany). Substrates 4-MU-(GlcNAc)2 (Exo-Chitinase activity ...
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bioRxiv - Systems Biology 2019Quote: ... The assay named ‘Other Luminex’ was performed only for study SLVP015 in 2007 using the Human 42-Plex Polystyrene Kit (EMD Millipore, H42; MPXHCYTO060KPMX42) and data was processed in the same way as for the Luminex assays described above (measurement units reported were Zlog2)28.
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bioRxiv - Developmental Biology 2019Quote: Apoptosis induced DNA damage was measured using the ApopTag® Peroxidase In Situ Apoptosis Detection Kit (cat no. S7100, EMD Millipore, Temecula, CA) following the manufacturer’s protocol for paraffin-embedded tissue ...
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bioRxiv - Developmental Biology 2019Quote: Fasting plasma samples collected from offspring at PN30 and during the GTT at 6-7 months of age were analysed for insulin levels using a rat insulin radioimmunoassay kit (SRI-13K, Millipore Australia, Kilsyth, VIC). Insulin samples were run in duplicate at 1:5 ...
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bioRxiv - Pathology 2020Quote: Total RNA was extracted from four leaf discs per replicate (three replicates, each sampled from two independent flood-inoculated ‘Hort16A’ plantlets) via a Spectrum™ Plant Total RNA kit (Sigma Aldrich, NZ). RNA was DNase I (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... Hybridization and all other procedures followed the manufacturer’s instructions using the DIG High Prime DNA Labeling and Detection Starter Kit-I (Sigma Aldrich, St. Louis, MO).
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bioRxiv - Physiology 2020Quote: ... was assessed in serum/plasma collected from lean and obese ZSF1 rats at the age of 20 weeks by rat-specific MILLIplex kit (Millipore, Billerica, MA, USA). Kidney injury markers KIM-1 and NGAL were evaluated in serum from 20-week-old lean and obese ZSF1 rats by multiplex MILLIplex kits (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: Larvae were processed as described (121) using the DuoLink Mouse Rabbit in situ PLA Kit (Sigma-Aldrich, cat. no. DUO92101, St. Louis, MO). Controls were performed with one or the other epitope-tagged transgene absent or with the probes replaced by water during the first PLA step to ensure that signal observed as not background or bleed-through of channels ...
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bioRxiv - Pathology 2021Quote: All samples were stained at the same time under the same conditions using a modified Fontana-Masson Kit staining protocol (Sigma-Aldrich Procedure #HT200). Prior to staining ...
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bioRxiv - Microbiology 2020Quote: ... 200ml of culture grown to stationary phase was concentrated using centrifugation (27,000 × g for 15 minutes) and extracted with the GenElute Bacterial Genomic DNA Kit (Millipore Sigma, Burlington, MA, USA). Basecalling was done using Guppy version 2.2.3 and long reads were filtered using filtLong version 0.2.0 (https://github.com/rrwick/Filtlong) ...
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bioRxiv - Genetics 2019Quote: ... Total RNA for both root and shoot tissues was extracted using the Spectrum™ Plant Total RNA Kit (Sigma-Alridch, Carlsbad, California, USA) using the manufacturer’s recommended protocol ...
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bioRxiv - Plant Biology 2021Quote: ... The pellet was discarded and supernatant was used to analyze the GST activity (Thounaojam et al. 2012). GST activity was performed according to method (Habig et al. 1974) by utilizing GST Assay kit (Sigma, USA, CS0410-1KT)
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bioRxiv - Microbiology 2020Quote: ... Pellets were processed according to the B protocol of the NXTRACT kit (CelLytic™ NuCLEAR™ Sigma-Aldrich, Milan, Italy https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/nxtractbul.pdf), in order to obtain native nuclear proteins ...
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bioRxiv - Biochemistry 2020Quote: ... concentrations in the different culture media were determined by the Bio-Plex method using the mouse adipocyte magnetic bead panel kit (Millipore, MADCYMAG-72K-07), according to manufacturer’s instructions (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then clarified by centrifugation and the protein content of each supernatant was determined using the Quanti-Pro bicinchoninic acid (BCA) assay kit (Sigma-Aldrich, MO, USA). Equal protein amounts (100 μg ...
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bioRxiv - Biochemistry 2021Quote: The ATPase activity of wild-type LonP1 and its mutants was measured with ATPase/GTPase activity assay kit (Sigma-Aldrich, USA, product: MAK113) as per the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: Sample preparation - In-gel digests were performed for each excised sample using the ProteoExtract All-in-One Trypsin Digestion Kit (Cat. No. 650212; Calbiochem, EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Masson’s trichrome Staining was applied according to the manufacturer’s instructions of the Trichrome Stain (Masson) Kit (HT15-1KT, Sigma-Aldrich, Heatherhouse, United Kingdom). For Safranin O/Fast green staining ...
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bioRxiv - Cancer Biology 2021Quote: ... EdU detection reagents were then added for 2 hours at room temperature in the dark (Click-iT™ Assay Kit, Sigma-Aldrich #C10337). Nutlin was used as a positive control (Selleckchem #S7101) ...
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bioRxiv - Cell Biology 2021Quote: The peptide/ZO1 interaction was detected in situ using the Duolink secondary antibodies and detection kit (Sigma, St Louis, MO, Cat: 92002, 92004) according to manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... The pyrosequencing assay was validated using SssI-treated human genomic DNA as a 100% methylation control and human genomic DNA amplified by GenomePlex Complete Whole Genome Amplification kit (Sigma-Aldrich, WGA2-50RXN) as 0% methylation control ...
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bioRxiv - Microbiology 2022Quote: ... The iron content of pre and post Chelex® 100 treatment was determined using the Iron Assay Kit (Sigma-Aldrich, MA, United States) following the manufacturer’s recommendations (Table S5).
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bioRxiv - Cell Biology 2022Quote: ... Conditioned media was run on the MilliPLEX Human High Sensitivity T Cell Magnetic Bead Panel Luminex kit for measurement of 21 unique cytokines per plex according to manufacturer’s instructions (EMD Millipore, St. Louis, MO).
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bioRxiv - Microbiology 2022Quote: ... Serum cytokine levels were measured by the Indiana University Multiplex Analysis Core using a Millipore Milliplex MAP Mouse Cytokine/Chemokine Magnetic Kit (Millipore Sigma, Burlington, MA). Undiluted serum was provided to the Indiana University Multiplex Analysis Core and ran according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Gram staining of the LT0009 isolate was performed using a Gram-staining kit according to the manufacturer’s instruction (Sigma Aldrich, 77730-1KT-F) and its cellular morphology was imaged with a scanning electron microscope (JSM-IT300 ...
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bioRxiv - Molecular Biology 2023Quote: ... The senescence condition in the two cellular models was assessed by monitoring the morphological changes and by β-galactosidase (β-gal) staining with the Senescence Cells Histochemical Staining Kit (CS0030, Sigma-Aldrich). After reaching the preferred condition ...
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bioRxiv - Neuroscience 2023Quote: ... Genotypes of the offspring were verified by PCR amplification of genomic ear DNA using the commercially available REDExtract-N-Amp™ Tissue PCR Kit (#R4775; Sigma-Aldrich, Germany) and primers designed to identify the presence or absence of the knock-in allele ...
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bioRxiv - Neuroscience 2023Quote: The TUNEL assay was performed on paraffin-embedded sections and fifty-micron-thick free-floating frozen sections according to the manufacturer’s instructions but with the time of some steps lengthened to ensure good penetration of the reagents into the tissue (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Millipore-Sigma, Cat # S7110). Sections were post-fixed for ten minutes at room temperature in 2:1 ethanol:acetic acid ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in biotinylated anti-rabbit secondary antibody 1:500 (Vectors Laboratories, #BA-1000) followed by Avidin/Biotin complex amplification (Vectastain Elite ABC kit; Vectors Laboratories, #PK-6100) and DAB (Sigma Life Science, #5905) mixed with 3% hydrogen peroxide for signal amplification ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lines were regularly checked for mycoplasma contamination using Venor™ GeM Mycoplasma Detection Kit (Sigma-Aldrich -Aldrich Co., Catalog no. MP0025-1KT).
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bioRxiv - Bioengineering 2024Quote: ... Imaging signal was visualized using imaging kits (SuperSignal™ West Pico PLUS Chemiluminescent Substrate or SuperSignal™ West Femto Maximum Sensitivity Substrate, Sigma-Aldrich) via an Image Quant LAS 4010 chemiluminescence imager (GE Healthcare) ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted (from TNBC cell lines or cryopreserved tumor tissue) using the GenElute mammalian total RNA miniprep kit (Sigma/Merck, RTN350-1KT) according to manufacturer’s instructions with an additional step of DNA digestion using the On-Column DNAse I digestion set according to manufacturer’s instructions (Sigma/Merck ...
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bioRxiv - Cancer Biology 2024Quote: PLA was performed to detect protein-protein interactions in MDA-MB-231 cells using Duolink™ In Situ Red Starter Kit (Sigma, cat. DUO92101). All the reagents ...
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bioRxiv - Genomics 2024Quote: ... Medium containing supplements were re-fed every 3-4 days for a total of 12 days after which the cells were evaluated for morphological changes and stained for tartrate-resistant acid phosphatase (TRAP) with a commercially available leukocyte acid phosphatase kit (SIGMA, Cat. 387-A). Three replicates of differentiated cells were processed to prepare samples for ATAC-seq at 0 ...
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bioRxiv - Synthetic Biology 2023Quote: Measurements of phosphate concentration in the growth medium supernatant were done using a Malachite green phosphate assay kit (Sigma-Aldrich, Cat. No. MAK307). After collecting the supernatant ...