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Citations for Millipore Sigma :
4001 - 4050 of 7743 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich, Pool, UK) using bovine serum albumin as standard ...
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bioRxiv - Molecular Biology 2022Quote: ... Total or nuclear protein was extracted using a nuclei isolation kit (NUC201, Sigma-Aldrich). Protein samples were electrophoresed on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene fluoride (PVDF ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... for each IP reaction 50 μl of Protein A agarose suspension (Merck Millipore, IP02) was incubated with 1.5 μg of GFP antibody (mouse anti-GFP ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein complex was eluted in Strep Buffer supplemented with 2.5 mM desthiobiotin (Sigma) at 1 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: ... and the proteins were then digested with 10 µg/mL proteinase K (Sigma-Aldrich) at 55 °C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins on gels were transferred on PVDF membrane (Immobilon-P, Millipore, Cat no. IPVH00010) and blocked with 5% non-fat dry milk (Scientific ...
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bioRxiv - Pathology 2022Quote: ... the incubated protein mixture was collected and centrifuged using centrifugal filters Utracel-100K (Millipore) at 500 × g for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... The total ubiquitinated proteins were detected by anti-FLAG (F-3165, 1:5,000, Sigma) and α-MYC (ab32 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were resolved by and blotted on Transfer membrane (Immobilon-P, Merck Millipore IPVH00010). Membrane blocking (5% BSA/TBS ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (EMD Millipore, catalog no. WBKLS0500) and ChemiDoc Imaging System (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich, Germany).
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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bioRxiv - Plant Biology 2024Quote: ... The separated proteins were transferred to Immobilon®-P PVDF membrane (Merck Millipore, USA) and probed with primary antibodies overnight ...
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bioRxiv - Microbiology 2024Quote: Incubation mixtures (30 µl) contained 10 µM of protein and 5× SYPRO Orange (Sigma) in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... The expression of proteins was induced with tetracyclin (1 µg/mL; Sigma-Aldrich, T7660). Cell were treated with actinomycin D (10 ng/mL ...
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bioRxiv - Cell Biology 2024Quote: PLA in cultured PAEC were performed using Duolink PLA protein detection technology (Millipore Sigma) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The dialyzed protein solution was filtered through a 0.22 μm membrane (Merck Millipore, SLGP033RB) and injected onto an ion-exchange Q column (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... bound proteins were eluted in TBS buffer supplemented with 5mM d-desthiobiotin (Sigma Aldrich). Affinity-purified proteins were incubated with HRV-3C protease to remove purification tags and subsequently purified by size-exclusion-chromatography on a Superose 6 Increase column (GE Healthcare ...
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bioRxiv - Bioengineering 2024Quote: ... Membranes were stained for total protein using Ponceau S (Sigma-Aldrich; St. Louis, MO) and subsequently probed with Aldehyde dehydrogenase 1-A1 ...
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bioRxiv - Molecular Biology 2024Quote: C2C12 protein was extracted from cells using RIPA buffer (Sigma-Aldrich, UK. Cat # R0278) using the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: mESC protein samples were isolated from mESC pellets using ice-cold RIPA buffer (Sigma) in the presence of freshly added 1% protease and phosphatase inhibitor cocktail (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: Proteins was isolated from cultured cells or brain tissues with RIPA buffer (Sigma, R0278) including protease inhibitors cocktails ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were eluted with wash buffer containing 1.5% β-mercaptoethanol (Sigma-Aldrich, M3148) at 37 °C for 0.5 h with agitation (350 rpm ...
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bioRxiv - Microbiology 2024Quote: ... Purified proteins were concentrated using 10 kDa cutoff Amicon Ultra Centrifugal Filters (Merck Millipore) and stored in 50% v/v glycerol at -20 °C until further use.
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
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bioRxiv - Molecular Biology 2024Quote: ... biotinylated proteins bound to streptavidin beads were resuspended in 100 mM ammonium bicarbonate (Sigma) and digested with 2 µg Trypsin (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were reduced using 5 mM tris (2-carboxyethyl) phophine hydrochloride (TCEP) (Sigma; 75259) for 10 min at 95° C and alkylated using 10 mM 2-iodoacetamide (Sigma ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... The eluted proteins were further concentrated using Amicon® Ultra-4 centrifugal filter (Millipore) and contaminants were removed using Gel-filtration chromatography ...
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bioRxiv - Neuroscience 2024Quote: ... 100 µg of proteins were reduced with 10 mM dithiothreitol (Sigma-Aldrich, MO, USA) at 37 °C for 60 min ...
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bioRxiv - Cell Biology 2024Quote: ... the specific proteins were visualized using a chemiluminescence HRP Substrate system (Millipore, Massachusetts, USA) and LAS 4000 (Fujifilm Life Science ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was eluted with 100 µL of 1.5 mg/mL FLAG peptide (Millipore) resuspended in wash buffer ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto 0.45 nm pore polyvinylidene fluoride (PVDF) membrane (Millipore, USA) using an eBlot™ L1 wet transfer (GenScript Biotech ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were precipitated by adding 100 μl of gamma-globulins (10 mg/ml; Sigma) and 2 ml of 20% TCA followed by centrifugation at 2,000 r.p.m ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The crude protein lysate concentration was quantified by bovine serum albumin (BSA) (Sigma-Aldrich) and 25 μg crude extract was loaded on NuPAGE™ 3-8% Tris-Acetate gels (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were concentrated using 10 kDa cut off Amicon centrifugal filters (Millipore, USA).
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bioRxiv - Pathology 2024Quote: ... the resolved protein samples were transferred onto a PVDF membrane (Merck Millipore Ltd., USA) through a Bio-Rad electrophoretic blotting apparatus ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were concentrated by centrifugation through Amicon Ultra 15 filter units (Millipore, Burlington, MA) with a 30-kD cutoff and then dialyzed against 3×PBS ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing cleaved proteins was concentrated using a 30K MWCO concentrator (EMD Millipore) to 1 ml ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins on the membranes were detected by enhanced chemiluminescence reagents (WBKLS0500, Millipore, USA).
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bioRxiv - Genetics 2024Quote: ... Then the resolved proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore, IPVH00010). The membranes were blocked ...
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bioRxiv - Immunology 2024Quote: ... protein bands on the blots were developed with chromogenic substrate SigmaFast BCIP/NBT (Sigma). All blots were run at least twice to check for reproducibility ...
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred to a 0.2 μm polyvinylidene fluoride membrane (PVDF, Millipore, Billerica, USA) using a Bio-Rad semi-dry instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were semi-dry blotted onto a 0.2 µM nitrocellulose membrane (Sigma cat GE10600001) and protein levels were detected by specific antibodies directed against ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were separated by SDS-PAGE and were transferred to PVDF membranes (Millipore). All membranes were incubated with the indicated primary antibodies diluted in TBS-T (20mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2024Quote: ... iFluor 647-conjugated sAvPhire monovalent streptavidin (catalytic soluble protein uptake, Millipore Sigma SAE178-100UG); iFluor 488-conjugated sAvPhire monovalent streptavidin (catalytic soluble protein uptake ...
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bioRxiv - Bioengineering 2024Quote: ... iFluor 488-conjugated sAvPhire monovalent streptavidin (catalytic soluble protein uptake, Millipore Sigma SAE176-100UG); IRDye 680RD Donkey anti-goat (Western blotting ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were eluted with 50 µg/mL of 1x FLAG peptide (Sigma-Aldrich #F3290), concentrated using 30 kDa centricons (Millipore #UFC9030) ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated using Amicon Ultra-0.5 10K Centrifugal Filter Devices (Merck Millipore). The protein concentration was measured on a Nanodrop 1000 spectrophotometer at 280 nm using the extinction coefficient of Trp and Tyr residues in the GST-LC3B sequence (48820 cm-1M-1).
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bioRxiv - Cancer Biology 2023Quote: ... secondary antibodies were added and the proteins were detected using an ECL kit (Millipore). Immunoreactive signals were visualized using the GE Amersham Imager 600 chemiluminescence system.
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bioRxiv - Biochemistry 2024Quote: ... Elution step of enriched proteins was performed using 2.25 M ammonia solution (Sigma, #5.33003) for 16 h ...