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Citations for Millipore Sigma :
4101 - 4150 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... ExtrAvidin® Peroxidase and 3-amino-9-ethylcarbazole (AEC) substrate solution (Sigma Aldrich) was used to visualize brown immune complex reaction product ...
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bioRxiv - Cell Biology 2024Quote: ... Treated films were immediately incubated in 5% (3-Aminopropyl)triethoxysilane (APTES, Sigma, 440140) in ethanol (dark ...
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bioRxiv - Biochemistry 2024Quote: ... enriched with 100 mM Dithiothreitol (DTT, CAS No. 3483-12-3, Sigma Aldrich). Samples were then heated at 95 °C for 8 minutes to ensure denaturation ...
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bioRxiv - Biochemistry 2024Quote: ... and 5-Bromo-4-chloro-3-indolyl phosphate-toluoidine were from Sigma-Aldrich, St ...
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bioRxiv - Cancer Biology 2024Quote: ... The fluorescent protein signal was then photobleached using 3 % H2O2 (Sigma-Aldrich, H1009) with 20 mM HCl (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Two volumes of 3 % (w/v) low-melt agarose (Sigma-Aldrich, Darmstadt, Germany) were added ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes with proteins were blocked with 3% (w/v) skim milk (Sigma, 70166) in Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Endogenous peroxidase activity was blocked using 3% Hydrogen peroxide (Sigma-Aldrich, Missouri USA) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... sodium acrylate (SA, 97–99%, 408220, SIGMA and 7446-81-3, AK Scientific), ammonium persulfate (APS ...
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bioRxiv - Cell Biology 2024Quote: Individual larvae were anesthetized with tricaine (0.03% ethyl 3-aminobenzoate methanesulfonate salt, Sigma). The larvae were then mounted on to glass-bottom dishes (35 mm Iwaki ...
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bioRxiv - Developmental Biology 2024Quote: ... FBS blocking buffer was used (3% fetal bovine serum (FBS; Sigma-Aldrich, 172012), 0.4% Triton X-100 in PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were treated with sequential injections of 3 μM oligomycin (Sigma, O4876), 1 μM carbonyl cyanide 4-(trifluoromethoxy ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed with PBST 2-3 times and bands were visualized upon addition of SigmaFast BCIP®/NBT tablets (Sigma B5655) (Fig ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were restreaked onto SD/-Trp/-Leu and incubated at 30 °C for 2–3 days before performing a colony-lift β-galactosidase assay (X-gal, Sigma-Aldrich) according to the Yeast Protocols Handbook (PT3024-1 ...
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bioRxiv - Cell Biology 2021Quote: ... Oocytes were surgically removed from anesthetized frogs (40, 46) under anesthesia (2 g/L Tricain, 3-aminobenzoic acid ethyl ester, Sigma A-5040). Preparation of defolliculated oocytes was carried out as described in (106) ...
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bioRxiv - Biophysics 2020Quote: ... oocytes were incubated twice (1st 45 min, 2nd variable) in OR-2 containing 3 mg/ml collagenase type IV (Sigma, C-9891). Defolliculated oocytes were stored in ND96 solution containing (in mM) ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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bioRxiv - Developmental Biology 2021Quote: Larvae at 2-6 dpf were first anesthetized with 0.03 % Ethyl 3-aminobenzoate methane sulfonate salt (Sigma-Aldrich, St. Louis, MO, USA) and pinned onto a Sylgard-filled recording chamber (I-2450 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then cleared by replacing ethanol with ethyl cinnamate (ECi; Ethyl 3-phenyl-2-propenoate; Sigma-Aldrich, order no. 112372-100G). After 30-60 min ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3 times with 1x PBS and incubated for 2 hours with 5% goat serum (NGS -Sigma-Aldrich, G9023) in 1x PBS with 0.3% Triton (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: Kinase activity assay was carried out by mixing 2 μg of CORK1KD or GST-CORK1KD-G748E with 3 μg of myelin basic protein (MBP; Sigma-Aldrich, Germany) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... logarithmically growing vps4Δ CDC48 or cdc48-3 cells were shifted to 37 °C for 2 h and simultaneously treated with 50 µM MG-132 (Sigma, Cat. # 474787) or the respective vehicle ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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bioRxiv - Molecular Biology 2023Quote: ... The locus with expected insertion was amplified by PCR using 5 μL KOD Hot Start 2× Master Mix (Sigma Aldrich 71842-3), 0.2 μL each primer at 10 μM ...
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bioRxiv - Immunology 2022Quote: ... type 2 and type 3 were mixed and subsequently concentrated using 10 kDa Amicon® Ultra Centrifugal Filters (Merck Millipore, Billerica, MA) to a nominal concentration of 10000-16000-32000 DU/mL.
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bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips was functionalized by amine using the silanization solution of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (Sigma-Aldrich, 8191720100), acetic acid ...
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bioRxiv - Cancer Biology 2024Quote: ... Target site on CCNB1 was amplified by a 2-step PCR using KOD XTREME HOT START DNA POLYMERASE (Sigma Aldrich #71975-3) using 25% of the total cDNA reaction above ...
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bioRxiv - Immunology 2024Quote: ... paraffin-embedded (FFPE) and blocks cut at 5µm (2-3 cuts per slide) and stained with Masson’s Trichrome stain (Sigma; Cat. No. HT15-1KT) following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... To the finely ground plant material 300 µL urea/DTT extraction buffer (8M urea/5mM DTT in 100mM Tris, pH 8.5/ protease inhibitor cocktail 2 and 3, Sigma, 20 µL/mL) was added ...
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bioRxiv - Microbiology 2020Quote: ... treated 3T3 mouse fibroblast feeder cells and grown until 70% confluency in the 3:1 mixture of Ham’s F12/DMEM media (HyClone) supplemented with 5% FBS (Sigma), 24 μg/mL adenine (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg of purified LDL were mixed with 50 µL DiI stock solution (3 mg/mL in DMSO; Sigma) and incubated at 37℃ for 8 h ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by selection in 1 μg/mL (C4-2B) or 1.5 μg/mL (DU145, PC-3) of puromycin (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-phospho-(1’-myo-inositol-4’,5’-bisphosphate) (ammonium salt) (PI(4,5)P2) (Sigma 850155P), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC ...
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bioRxiv - Neuroscience 2021Quote: ... Then mice were randomly infused 10 minutes before the test with 2µL (500nL m-1) of vehicle (∼3 % dimethyl sulfoxide (DMSO, Sigma) diluted in aCSF ...
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bioRxiv - Immunology 2020Quote: ... After incubation cells were washed 3 times with PBS containing 0.5% BSA and subsequently fixed 1% (w/v) paraformaldehyde (PFA, Merck Millipore) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1% Glycerol) containing a 3-fold dilutions (from 0.125 units/ml to 6 units/ml) of Benzonase nuclease (Millipore). This was mixed gently and incubated for three minutes at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... epitopes were blocked with 0.1 M PBS / 1% Cold Water Fish Skin Gelatin / 0.2% BSA with 3% sucrose (all chemicals from Sigma Aldrich), incubated with the primary antibody anti-GFP (1:250 ...
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bioRxiv - Biochemistry 2020Quote: ... 50 mM sodium phosphate pH 8.0, 3% glycerol, 1% Triton X-100, 15 mM imidazole, and 1x protease inhibitor [Roche, Sigma]) and sonicated ...
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bioRxiv - Cell Biology 2021Quote: ... cells were trypsinized than fixed with PBS/ formalin 3% then incubated with PBS/ Triton 0.1%/ TRITC-phalloïdin 1 µg/mL (Sigma Aldrich) during 5 min ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then washed twice with PBS prior to 20 min permeabilization with 0.5% Triton X-100 and 1 h blocking with 3% BSA (Sigma) at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... before 3 washes with 1 mL PBS and saturation with a 0.5% BSA solution in PBS 1X (Sigma A7888) for 15 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated in blocking buffer for 1 hour at 25°C (TBST with 3% BSA; Sigma) before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: Sox14GFP/+ mouse brain samples (n=3) were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... The cultures were then blocked for 1 hour at room temperature with 3% (w/v) BSA in PBS (Sigma), followed by incubation with anti-Gfap (1:100 ...
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bioRxiv - Cell Biology 2021Quote: Chromatin was isolated from supernatant by adding 3 volumes of TE with 1% SDS and RNAse A (Sigma R6513), and vortexing to mix ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK 293T cells were transfected with a 1:3 ratio of DNA:polyethylenimine (PEI, 25 kDa linear, Sigma-Aldrich). Transfection of siRNA was performed using X-tremeGENE siRNA Transfection Reagent (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated at RT for 1 hour in a blocking solution containing 3% Donkey Serum (Biowest) and 0.3% Triton X-100 (Sigma) in PBS 0.01M ...
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bioRxiv - Cancer Biology 2022Quote: Human melanoma cells were seeded in 6-well plates with 0.1-1 mL lentivirus in a final volume of 3 mL with 4 µg/mL polybrene (Millipore). Cells were spun at 1,000 x g for 2 h at 30 ºC ...
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bioRxiv - Immunology 2021Quote: ... at room temperature for 3 hours and followed by streptavidin-HRP (dilution 1:1000, Sigma-Aldrich, Cat# 18-152) for 45 minutes ...