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Citations for Millipore Sigma :
4201 - 4250 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and Anti-dsDNA antibodies were diluted 1/100 in blocking buffer (3 % bovine serum albumin (BSA; A7906, Sigma-Aldrich) in 1 x phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... GST and 3×FLAG-tagged proteins were detected with a rabbit polyclonal anti-GST antibody (1:2500, Sigma-Aldrich) and a mouse monoclonal HRP-conjugated anti-FLAG antibody (M2 1:10000 ...
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bioRxiv - Cancer Biology 2024Quote: ... mitotically active cells were identified using a rabbit anti-phosphohistone-3 antibody (Ser10, Millipore 06–570, 1:500 dilution), with a Cy3-donkey anti-rabbit immunoglobulin G secondary antibody (the Jackson laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... We used 3 and 5wt% GelMA with a 1:16 mass ratio of photoiniator (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate, LAP; Sigma Aldrich). LAP and GelMA were dissolved together in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Microbiology 2024Quote: ... The cell nucleus was stained with DAPI (1:1000 dilution, Sigma-Aldrich 28718-90-3, St. Louis, MO, USA) at room temperature for 15 min ...
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Evolution of polyamine resistance in Staphylococcus aureus through modulation of potassium transportbioRxiv - Microbiology 2024Quote: ... Four of the cultures were diluted 1/100 into fresh TSB containing 2mM of spermine (Sigma, 71-44-3), and two of the cultures were diluted 1/100 into plain TSB as a control ...
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bioRxiv - Molecular Biology 2024Quote: ... Selleck) in the early stages of differentiation and by its subsequent inhibition after 3 days (adding IWR-1; Sigma). Genome editing analyses on hiPSCs were performed by co-transfecting the PX333 plasmid57 ...
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bioRxiv - Immunology 2024Quote: ... and nanobodies were eluded via 3 × 1 mL 0.5 M imidazole in phosphate-buffered saline (PBS) (I2399, Sigma-Aldrich). An overnight dialysis (3 kDa MWCO ...
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bioRxiv - Genetics 2024Quote: ... 10-200 mg tissue was digested in 1 ml 3 N hydrochloric acid (Fisher)/10% trichloroacetic acid (Millipore Sigma) at 65 °C for two days ...
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bioRxiv - Bioengineering 2024Quote: ... Primary cells were cultured in basal medium ((DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific], 0.4 μL/mL hydrocortisone [Sigma] ...
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bioRxiv - Developmental Biology 2024Quote: Embryos aged 24-28 hpf and 3 dpf were paralyzed with 1 mg/mL α-Bungarotoxin (Sigma- Aldrich, 203980) for 15 minutes to block nicotinic acetylcholine receptors and prevent motion artifacts during imaging ...
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bioRxiv - Bioengineering 2024Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific], 0.4 μL/mL hydrocortisone [Sigma] ...
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bioRxiv - Cell Biology 2024Quote: ... All anaesthesia was conducted with 0.2 mg ml−1 3-amino benzoic acid ethyl ester (Sigma, MS-222, E10521), (pH=7.0) ...
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bioRxiv - Developmental Biology 2024Quote: ... Fixed larvae (3-14 dpf) and dissected and cleared brains were mounted in 1% low gelling agarose (Sigma: A9414) on 0.17 mm coverslips and imaged on either a Leica SP8 or Stellaris DMI8 laser scanning confocal microscope at 1024×1024 resolution using the same acquisition settings with a 25x water immersion objective.
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bioRxiv - Microbiology 2020Quote: ... primary antibodies were mouse-anti-ROP2/3/4 (1:250 dilution, 4A7, (58)) and rat anti-HA (1:500, clone 3F10, Sigma Aldrich, 11867431001), and secondary antibodies were goat anti-mouse IgG-Alexa 647 (1:2000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were pelleted at 4000 g for 10 mins and resuspended in 1 mL of freeze thaw lysis buffer (PBS, 1 mg/ml lysozyme, 0.5 mM EDTA and 1 μl/ml of benzonase (Millipore, catalog #70746-3)) ...
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bioRxiv - Neuroscience 2024Quote: ... Specimens were then immersed twice in 100% methanol for 1 hour and treated overnight in 1/3 volumes of 100% dichloromethane (DCM; Sigma-Aldrich; 270997). The subsequent day ...
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bioRxiv - Molecular Biology 2024Quote: ... The rate of O2 change was measured every 13 minutes for a 3-minute interval before sequential challenge with 1) 1 μM oligomycin (Sigma-Aldrich: 75351), 2 ...
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bioRxiv - Physiology 2024Quote: ... The rate of O2 change was measured every 13 minutes for a 3-minute interval before sequential challenge with 1) 1 μM oligomycin (Sigma Aldrich 75351), 2 ...
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bioRxiv - Microbiology 2023Quote: ... Blood was mixed at a 1:1 ratio with a filter-sterilized solution containing 3% dextran and 0.9% sodium (Sigma-Aldrich, Burlington, MA). Red blood cells sedimented and fell to the bottom of the tube ...
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bioRxiv - Neuroscience 2022Quote: ... Then the samples were washed 3 times with PBS and then incubated for 1 hour with the donkey α mouse Cy3 (1:200; Millipore Chemicon), donkey α rabbit Cy5 (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting collagen I-thrombin solution was then mixed in a 1:1 ratio with 3 mg/mL bovine fibrinogen (Sigma, Cat. # F8630) solution in PBS ...
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bioRxiv - Biophysics 2023Quote: ... We washed the membrane 3 times with TBST before incubating with a 1:1 ratio of horseradish peroxidase substrate and enzyme mixture (Millipore, Burlington, Massachusetts). We imaged using a Biorad Chemidoc camera set to detect chemiluminescence ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used for immunofluorescence were: anti- cleaved Caspase-3 (Asp175) (1:400, rabbit, CST, #9661) and anti-FLAG (1:400, mouse, Sigma-Aldrich, #F3165) for detection of overexpressing proteins ...
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bioRxiv - Genetics 2021Quote: ... Zebrafish embryos and larvae were anesthetized in 160ug/ml Ethyl 3-aminobenzoate methanesulfonate (Tricaine, MS-222) C9H11NO2·CH4SO3 (Sigma-Aldrich, 886-86-2) in E3 embryo media (Westerfield ...
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bioRxiv - Immunology 2021Quote: ... Minced heart tissue was digested in 3 mL of digestion buffer (2 mg/mL Collagenase IV [Worthington Biochemical], 1.2 units/mL Dispase II [Sigma-Aldrich], in perfusion buffer) for ∼45 minutes at 37° C using a rotating holder ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were performed in 6 μL volumes on 384-well plates in reaction buffer containing 3 μL of 2 x Kapa Probe Fast qPCR Master Mix (Sigma-Aldrich, Dorset, UK). All probes were supplied by Thermo Fisher Scientific (Massachusetts ...
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bioRxiv - Neuroscience 2024Quote: ... at least 3 times and incubated at room temperature for 2 h in blocking solution containing 5% normal horse serum (Sigma-Aldrich, Cat. # H0146) in 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... In order to substitute cholesterol in the patch with cholest-4-en-3-one (cholestenone) the bath solution was replaced with 2 mM MBCD solution saturated with cholest-4-en-3-one (Millipore-Sigma, St. Louis, MO).
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bioRxiv - Neuroscience 2024Quote: ... aCSF and 192IgG-SAP groups received intraperitoneal (ip) injections of WIN55,212-2 (0.5 mg/kg or 3 mg/kg) (Sigma-Aldrich, St. Louis, MO, USA) or vehicle solution (1:1:18 ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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bioRxiv - Cancer Biology 2023Quote: ... 2-4 months old mice were orally gavaged with 3 x 2 (Mist1creERT/+KRASG12D/+) or 5 x 5 mg (Ptf1acreERT/+KRASG12D/+) of tamoxifen (TX; Sigma-Aldrich, T5648-5G) at Western University and 5 x 4 mg (Ela-creERT ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were permeabilized with 0.2% Triton X-100 (Nacalai Tesque, Kyoto, Japan) for 15 minutes and blocked with 3% bovine serum albumin (BSA: Sigma Aldrich, MO, USA) for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... a RRP17-HA-AID strain was transformed with plasmids carrying respective constructs and growth assessed upon Rrp17-HA-AID depletion on plates containing 2 mM auxin (3-indoleacetic acid) (Sigma-Aldrich, Cat# I2886). Genetic experiments were performed at least in two biological replicates.
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bioRxiv - Immunology 2024Quote: ... The liver was chopped into small pieces (2 to 3 mm) and crushed with a syringe plunger before the collagenase D (1mg/ml in complete RPMI; Sigma life science, #11088882001) digestion 10 min at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated with Amicon® 3 kDa MWCO concentrator (Millipore, Burlington, MA) and injected into a 10/300 Superdex 75 size-exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with protease inhibitor cocaktail (P8340) and phosphatase inhibitor cocktail 3(Milipore Sigma, P0044)) ...
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bioRxiv - Microbiology 2020Quote: LB-agarose pads were prepared by boiling 1.5% or 3% ultrapure agarose (Sigma Aldrich) in LB ...
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bioRxiv - Microbiology 2020Quote: LB-agarose pads were prepared by boiling 1.5% or 3% ultrapure agarose (Sigma Aldrich) in LB ...
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bioRxiv - Microbiology 2020Quote: ... 13 mM glycine and 3 μM thiamine (all compounds from Merck Millipore, Darmstadt, Germany).
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bioRxiv - Cell Biology 2020Quote: ... the silicon nitride cantilevers were functionalized with (3-glycidyloxypropyl)trimethoxysilane (98%, Sigma Aldrich, 440167) at 80 °C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... Larvae were anesthetised with 2.5% (v/v) ethyl-3-aminobenzoate methanesulfonate (tricaine) (Sigma, E10521), wounded posterior to the notochord using a sterile scalpel and kept in a 28°C incubator to recover as previously described (27) ...
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bioRxiv - Neuroscience 2020Quote: ... 2000) containing 0.3% ethyl 3-aminobenzoate methanesulfonate (Tricane; MS-222(Cat E10521, Sigma-Aldrich)) ...
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bioRxiv - Developmental Biology 2021Quote: Adult mice (3-7.5 months) were treated with Angiotensin II (cat. #A6402, Sigma-Aldrich) for two weeks ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP, Sigma Israel, B8503). In the foxd3/Rspo1 ISH experiment ...
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bioRxiv - Cell Biology 2022Quote: ... immediately followed by a coating of 3- (trimethoxysilyl)propyl methacrylate (TMS) (Sigma-Aldrich, USA) to methacrylate the glass surfaces (Guvendiren et al. ...
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bioRxiv - Neuroscience 2021Quote: Tadpoles were briefly anesthetized with 0.1% MS-222 (ethyl 3-aminobenzoate methanesulfonate, Sigma-Aldrich), and subsequently immobilized by immersion in a 10 μM α-bungarotoxin (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... 500 μl PBS (PBST) containing 3% normal donkey serum (S30-100ML, Millipore, Hertfordshire, UK) at room temperature (RT ...
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bioRxiv - Physiology 2021Quote: ... rAAVs were washed 3 times with PBS using Amicon Ultra Centrifugal Filter Units (Millipore) and resuspended in PBS + 0.001% Pluronic F68 ...