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Citations for Millipore Sigma :
4151 - 4200 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked with 3% w/v BSA and incubated with 1:16,000 v/v rabbit anti-protein A antibody (SIGMA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Band intensity was measured as the sum optical density by using ImageJ software (version1.4; NIH) and normalized to control labelling of glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:5000, Millipore).
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bioRxiv - Molecular Biology 2022Quote: ... 1-3 million cells were treated for 24 hours with 1.2 uM aphidicolin in PBS (Millipore Sigma cat#: A0781). Cells were attached to Concanavalin A coated slides for one hour at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... The primary antibody (1:100 Anti-Tau [3-repeat isoform RD3] Antibody, clone 8E6/C11, 05-803, Sigma-Aldrich and 1:100 Anti-Tau [4-repeat isoform RD4] Antibody ...
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bioRxiv - Microbiology 2022Quote: ... A total of 500 μL of normalized culture was mixed with 100 μL of 1% Zwittergent 3-14 (Sigma) in triplicate in 100 mM citric acid and incubated at 50 °C for 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... EpiSCs were passaged 1:4–1:10 every 3 days by triturating the colonies into small clumps using 0.5 mg ml–1 collagenase IV (Sigma). MEK/WNT-inhibited RSCs were cultivated with 1,000 U ml–1 LIF ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM DTT) and treated with 3×45 s bead beating with glass beads (particle size≤106 μm Sigma) at maximum power and cooling on ice between the cycles (MP Biomedicals™ FastPrep-24™ ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was initiated at the spectrophotometer by adding anoxic xanthine oxidase (microbial; 1-3 µg/mL; Sigma-Aldrich) via syringe and needle through the septum lid ...
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bioRxiv - Developmental Biology 2020Quote: Cryosections (30-40 μm) were blocked with PBS/1-3% normal goat or donkey serum/0.3% Triton X-100 (Sigma) for up to 1 hour at room temperature (RT ...
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bioRxiv - Genetics 2020Quote: Total RNA from 7 DAP spikes was extracted using TRI Reagent and 1-bromo-3-chloropropane (BCP) (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... a mouse anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (#MAB374, used at 1:10,000 for the Western blot analyses, Merck Millipore); a rabbit anti-LC3B polyclonal antibody (#2775 ...
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bioRxiv - Cell Biology 2020Quote: ... permeabilized with 0.1% Triton X-100 in PBS for 3 min and blocked in PBG (PBS, 0.045% cold fish gelatin (Sigma), and 0.5% BSA) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were positioned on gelatin-coated slides to allow further development with 3:1 ammonia solution (1054261000, Milipore Sigma) for 8 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... 293T or AL1 cells were incubated for 3 hours with anti-Myc mouse monoclonal antibody (Sigma-Aldrich; 1:500), and secondary staining was performed using anti-mouse AlexaFluor568 antibodies (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed in 1% PFA (10 min, 37°C) and permeabilized in permeabilization buffer (PBS 3% BSA, 0.1% saponin, Sigma) for 5 min ...
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bioRxiv - Genetics 2020Quote: ... incubated for 3 hours at 25 °C with the secondary antibody (anti-rabbit IgG peroxidase 1:10000, Sigma-Aldrich; anti-mouse IgG peroxidase 1:10000 ...
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bioRxiv - Immunology 2022Quote: ... and blocked for 1 hour at room temperature with 100 μL of 3% globulin free BSA (Sigma-Aldrich, A7030). After blocking ...
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bioRxiv - Developmental Biology 2022Quote: ... fused gastruloids were induced by administration of 3 μM CHIR and if indicated with doxycycline (1 μg/μl, Sigma) for 24 h ...
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bioRxiv - Biochemistry 2022Quote: ... The incubated beads were washed 3 times with HAC buffer followed by eluted in 1 M imidazole (Sigma, #IX0005) in HAC buffer.
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bioRxiv - Cell Biology 2022Quote: ... The beads were washed with IP lysis buffer and incubated with 5 mg ml−1 3× FLAG peptide (Sigma–Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Systems Biology 2023Quote: Buffy Coat was diluted with PBS at a 1:3 dilution and underlayed with Histopaque®-1077 (Sigma-Aldrich), centrifuged at 350g for 30 minutes at 24°C with no brake ...
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bioRxiv - Neuroscience 2023Quote: ... 100 μg/mL streptomycin and 1 mM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic sodium monophosphate salt (dbcAMP) (Sigma®). Cells were maintained with the differentiation medium at 37 °C in 5% CO2 for 3 days.
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bioRxiv - Physiology 2022Quote: ... cells were treated with the medium supplemented with 0.5 mM 3’-Isobutyl-1-methylxanthine (IBMX) (Sigma, Cat. I7018-1G), 1 µM dexamethasone (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times with DPBS for 5 min and blocked in 1% BSA (Sigma-Aldrich, cat# A4503) in PBS-T (DPBS with 0.05% Tween 20 ...
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bioRxiv - Developmental Biology 2022Quote: ... BP310-1], 10 mM NaCl [Fisher, Cat. M-11624], 3 mM MgCl2 [Sigma, Cat. M8226], 0.1% IGEPAL [v/v; Sigma, I8896] ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 mM HEPES-KOH, pH 7.2 [Fisher, Cat. BP310-1], 10 mM NaCl [Fisher, Cat. M-11624], 3 mM MgCl2 [Sigma, Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... were grown for 2 hours on 3 ml of LB with 0.2% arabinose before placing them on a patch consisting of 1% agarose (Sigma) in water ...
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bioRxiv - Neuroscience 2023Quote: ... washed with PBS and blocked at room temperature for 1 h using either 3% bovine serum albumin (BSA, Sigma) or 5% goat serum according to the primary antibody used ...
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bioRxiv - Genomics 2022Quote: ... An antibody specific for GroEL (rabbit polyclonal, 1:10,000 in 3% BSA/TBS-T; Sigma-Aldrich, #G6532-5ML, RRID:AB_259939) with an anti-rabbit IgG (goat polyclonal ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 h in 20 mL TBS-T with 3% milk powder and 1:10,000 diluted goat anti-rabbit IgG-HRP (Sigma). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... they were contained in the bath solution and then recorded using the following concentration: Gadolinium chloride (GdCl3) (0.1, 0.3, 1, 3, and 10 μM, Sigma, G7532), Na-gluconate (gluconate ...
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bioRxiv - Immunology 2023Quote: ... for 1 hour followed by 3 hours of stimulation using 50 nM phorbol myristate acetate (PMA, Sigma-Aldrich, P1585), 25 μg/ml Lipopolysaccharide (LPS ...
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bioRxiv - Genomics 2023Quote: ... The aqueous layer was separated using 50 μL 1-bromo-3-chloropropane (BCP; Sigma-Aldrich #B9673, Burlington, MA, USA) per 500 μL of Trizol and centrifuged at 12000 x g for 15 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: Mouse sera were diluted 1:100 in an assay buffer of 0.05% PBS-T supplemented with 3% (w/v) BSA (Sigma) and transferred into a 96-well plate ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were concentrated to ∼ 3-4 mg mL-1 using a 100 kDa MWCO centrifugal concentrator (Millipore Sigma) for cryo-EM sample preparation.
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bioRxiv - Genetics 2023Quote: ... 10-200 mg tissue was digested in 1 ml 3 N hydrochloric acid (Fisher)/10% trichloroacetic acid (Millipore Sigma) at 65 °C for two days ...
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bioRxiv - Bioengineering 2023Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific], 0.4 μL/mL hydrocortisone [Sigma] ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with an α-FLAG antibody (Sigma; 1:1,000 in 1x TBS-T containing 3% BSA) at 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Wells were washed with sterile PBS x 3 and cells were lysed with 0.25 mL of 1% (weight/volume) saponin (Sigma) for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... or 250 nM Compound 3 were fixed and stained for mouse anti-γ-tubulin 1:500 (Sigma Aldrich, T5326), rabbit anti-KIF18A 1:100 (Bethyl ...
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bioRxiv - Neuroscience 2023Quote: ... A 1-in-3 series was collected directly onto gelatin-coated slides and Nissl-stained (cresyl violet, Sigma-Aldrich) for verification of electrode location.
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bioRxiv - Cell Biology 2023Quote: ... and microtubules (1 µM paclitaxel, T7402, Sigma Aldrich; 33 µM nocodazole, M1404, Sigma Aldrich, 3 different commercial lots tested). The effect of nocodazole during overnight/short-term culture was enhanced by 10 µM verapamil (V4629 ...
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bioRxiv - Immunology 2023Quote: ... A total of 500 μL of normalized culture was mixed with 100 μL of 1% Zwittergent 3-14 (Sigma) in triplicate in 100 mM citric acid and incubated at 50 °C for 20 min ...
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bioRxiv - Genomics 2023Quote: ... 24 hours prior to induction the cells were cultured in 1 mM indole-3-acetic acid (IAA; Sigma, I5148), to ensure protein degradation ...
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bioRxiv - Immunology 2023Quote: ... and secondary antibodies were diluted in IF buffer and incubated for 1 hour at RT and washed 3 more times with PBS 0.05% Tween20 (Sigma). Sections were incubated with PBS DAPI (5 µg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... We used 3 and 5wt% GelMA with a 1:16 mass ratio of photoiniator (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate, LAP; Sigma Aldrich). LAP and GelMA were dissolved together in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Cancer Biology 2024Quote: ... mitotically active cells were identified using a rabbit anti-phosphohistone-3 antibody (Ser10, Millipore 06–570, 1:500 dilution), with a Cy3-donkey anti-rabbit immunoglobulin G secondary antibody (the Jackson laboratories ...
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Evolution of polyamine resistance in Staphylococcus aureus through modulation of potassium transportbioRxiv - Microbiology 2024Quote: ... Four of the cultures were diluted 1/100 into fresh TSB containing 2mM of spermine (Sigma, 71-44-3), and two of the cultures were diluted 1/100 into plain TSB as a control ...
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bioRxiv - Microbiology 2024Quote: ... The cell nucleus was stained with DAPI (1:1000 dilution, Sigma-Aldrich 28718-90-3, St. Louis, MO, USA) at room temperature for 15 min ...