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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The FLAG M2 antibody used for the detection of MetRSL274G protein was purchase from Sigma. ANL ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
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bioRxiv - Plant Biology 2023Quote: ... was used to detect proteins with horseradish peroxidase-conjugated anti-rabbit secondary antibodies (Sigma, A6154).
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bioRxiv - Pathology 2023Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473 ...
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bioRxiv - Plant Biology 2023Quote: ... The AGO1 protein was detected by Western blot using a Flag-HRP antibody (A8592, Sigma).
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bioRxiv - Developmental Biology 2021Quote: ... anti-Caspase-3 antibody (Sigma USA, Dubey and Tapadia, 2017) was used at a dilution of 1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... or anti-SMAD2/3 antibodies (Millipore, 07-408 - Burlington, MA).
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bioRxiv - Microbiology 2021Quote: ... rabbit anti-Akt 1/2/3 antibody (Millipore, Burlington, MA), rabbit anti-MAPK family antibodies (Cell Signaling Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit polyclonal antibody to GluR2/3 (1:100; #AB1506; Millipore) or a rabbit polyclonal antibody to NPY (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking and antibody staining was performed in 3% bovine serum albumin (BSA; Sigma-Aldrich; Cat. No. A7906) in PBST ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking and antibody staining was performed in 3% bovine serum albumin (BSA; Sigma-Aldrich® cat. # A7906) in PBST ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody was diluted in a solution of 3% bovine serum albumin (BSA; Cat#A7906, Sigma-Aldrich), 2% donkey serum (DS ...
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bioRxiv - Cancer Biology 2023Quote: ... The sample was stained with antibody against pH3 (phosphorylated histone 3, 1:800, Sigma-Aldrich 06-570) and H2 (Hemese ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-GAPDH antibody was used at a 1:2000 dilution with TBST containing 3% BSA (Sigma), and other primary antibodies were used at a 1:1000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... cells were biotinylated by incubation in biotin-free growth media supplemented with 50 µM biotin (Sigma-Aldrich). After exactly 20 minutes ...
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bioRxiv - Molecular Biology 2020Quote: HPG labeled proteins in supernatant and pellet fractions (∼1 mg) were click-conjugated with 50 µM azide-PEG3-biotin (Sigma-Aldrich) by a Click-iT Cell Reaction Buffer Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Equal amounts of SG core was subject to immunoprecipitation using anti-G3BP1 antibody (1ug) and isotype specific control antibody and protein A-sepharose beads (Sigma-Aldrich). Samples were incubated at 4⁰C overnight on a rotator and immune complexes recovered by centrifugation and five washes in buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were then washed 4x with Buffer A containing only 0.1% NP-40 and bound protein was eluted by 3 sequential incubations in PBS containing 0.1% triton x-100 (Sigma-Aldrich) and 0.5mg/ml of 3x FLAG peptide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Bound protein was eluted seven times with 0.5 column volumes of buffer A with 3 mM D-Desthiobiotin (Sigma-Aldrich), 0.026% DDM and 0.1 mM AEBSF protease inhibitor ...
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bioRxiv - Immunology 2022Quote: ... 3 mM 2-mercaptoethanol and the mixture was concentrated to 10 mg/ml (Protein concentration) using an Amicon Ultra-4 filter (3 kDa molecular-weight cutoff, Millipore). The FoxP3ΔN’–DNA complex was then mixed with the reservoir solution (0.1 M Tris-Hcl pH 8.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... degradation of V5-AID-fused Brn1 protein was induced by addition of 0.5 mM indole-3-acetic acid (Auxin, Sigma # 13750).
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bioRxiv - Plant Biology 2020Quote: ... proteins were eluted with 150 μg.ml-1 3× FLAG peptide (Sigma-Aldrich, F4799) in extraction buffer ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore) at 3500 rpm and 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 350 µg total protein concentrated with 3-kDa cut-off centrifugal filter (Millipore) from serum-free medium was subjected to SDS-PAGE with multiple band excision and in-gel digestion ...
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bioRxiv - Physiology 2023Quote: ... Proteins were then concentrated to approximately 3 mg/ml by ultrafiltration (Millipore, UFC905096) at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... The refolded proteins were concentrated with Amicon 3 kDa Ultracel filters (EMD Millipore). For GFP-PSB15 purification ...
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bioRxiv - Cancer Biology 2022Quote: ... and then for 1 h at room temperature with a mix of the two primary antibodies directed against the target proteins (anti-biotin to detect new H3.3-SNAP-biotin and anti-gH2A.X to detect sites of DNA damage) diluted in antibody dilution reagent (Sigma-Aldrich). Coverslips were then incubated for 1 h at 37 °C with secondary antibodies each harboring a PLA probe (Duolink In Situ PLA MINUS/PLUS probes ...
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bioRxiv - Cell Biology 2022Quote: ... Sodium hyaluronan (1.5MDa) was from Lifecore (Chaska, MN, USA) and hyaluronan biotin sodium salt was from Sigma. Casein blocking buffer (10× ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and HRP-conjugated Anti-Rabbit IgG Secondary Antibody) and R&D Systems/Minneapolis/MN (14-3-3-Sigma polyclonal goat IgG and HRP-conjugated Anti-Goat IgG Secondary Antibody) ...
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bioRxiv - Cell Biology 2019Quote: ... Bcl2/Adenovirus E1B 19 kDa and protein-interacting protein 3-like (BNIP3L/NIX) from Sigma-Aldrich (St. Louis, MO, USA), perilipin (PLIN ...
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bioRxiv - Immunology 2024Quote: ... 20 μg cell proteins or 3-5 μg EVs proteins were denatured in LDS sample buffer containing 1M Dithiothreitol (DTT, Sigma) for 10 min at 70°C and loaded onto a 4-12% precast polyacrylamide gels for electrophoresis ...
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bioRxiv - Biophysics 2021Quote: ... DNA-fluorophore constructs were immobilized on the surface using biotin-avidin as follows: BSA-biotin (A8549, Sigma-Aldrich) was dissolved and diluted in buffer A (10 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides contained a C-term PEG-Biotin and were synthesized using a preloaded PEG-Biotin resin (Sigma-Aldrich). Peptides were cleaved from the resin using TFA / Water / Thioanisole / Triisopropylsilane / Phenol / Ethanedithiol – 82.5/0.5/0.5/0.25/0.25/0.25 and precipitated into cold diethyl ether ...
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bioRxiv - Bioengineering 2020Quote: ... mPA (K563C, N682A, D683A) was modified with 10 equivalents of biotin-maleimide (Sigma Aldrich) at r.t ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... media was replaced with 2 mL DMEM supplemented with 50 μM Biotin (B4501, Sigma) (prepared as previously described [54] and 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... Excess biotin was then removed using Amicon Ultra 0.5.L columns (3K, Millipore Sigma) according to the instructions of the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... the aptamer-DBCO conjugate was replaced with DBCO-dPEG®12-biotin (Sigma-Aldrich), with the remaining assembly steps remaining the same ...
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bioRxiv - Cell Biology 2020Quote: Thymidine and biotin were from Sigma-Aldrich and rapalog was from Clontech.
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bioRxiv - Neuroscience 2020Quote: ... 20 μM biotin (Sigma-Aldrich Cat# B4501) was added to the culture medium after SCE transfection ...
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bioRxiv - Neuroscience 2020Quote: ... using Biotin RNA Labeling Mix (Sigma Aldrich). Prior to experiments ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ng/mL biotin (#B4639, Sigma-Aldrich), 5 µg/mL insulin (#I1882 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with biotin (Sigma Aldrich). Sections were then probed with anti-B220 (clone RA3-6B2 ...
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bioRxiv - Neuroscience 2022Quote: ... and Biotin (1 μg/mL,Sigma, B4501) were injected intravenously into C57 mice ...
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bioRxiv - Biophysics 2022Quote: ... stored and biotinylated using biotin-NHS (Sigma) as previously described ...
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bioRxiv - Biophysics 2022Quote: ... or biotin with PEG4 linker (Sigma Aldrich); or ∼0.15 mM oligo DNA ...
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bioRxiv - Bioengineering 2022Quote: ... biotin (50 μg.mL−1, Sigma, B4639, USA) and ZellSchield® 1X (ZellSchield® ...
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bioRxiv - Cell Biology 2022Quote: ... or NHS-dPEG®4-biotin (Sigma), the same steps were followed as RMA purification until pelleted filamentous actin was homogenized and sonicated ...
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bioRxiv - Biochemistry 2021Quote: A 2.05 mM biotin (Sigma-Aldrich B4501) stock was prepared in distilled water by brief sonication ...