Labshake search
Citations for Millipore Sigma :
251 - 300 of 10000+ citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... washed twice with 500 µL PBS and re-suspended in 500 µL of PBS containing ∼3 mg/mL Biotin-NHS (Sigma B1022). Cells were incubated for 30 min on a rotating wheel at room temperature ...
-
bioRxiv - Biophysics 2023Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (biotin-DOPE) (structures in Table S5) were purchased from Sigma-Aldrich (UK). Agarose powder was purchased from Invitrogen (UK) ...
-
bioRxiv - Cell Biology 2023Quote: ... the column was washed with 20 column volumes of the lysis buffer and the protein was eluted with 3 mM desthiobiotin (Sigma, D1411).
-
bioRxiv - Immunology 2019Quote: ... endogenous Asc from a total of 3.5 mg protein was immunoprecipitated with 1 μg of anti-Asc antibody and 60 μl of protein A/G agarose beads (Sigma), and bound Mul1 was detected with anti-Mul1 antibody by western blotting ...
-
bioRxiv - Cell Biology 2019Quote: ... Gene Tex) antibody was incubated with protein G coated magnetic beads (PureProteome protein G magnetic beads, LSKMAGG10, EMD Millipore Corp.) for 1-3 hrs in IP (immunoprecipitation ...
-
bioRxiv - Neuroscience 2020Quote: ... cell culture media was supplemented with 50 μM biotin (B4639, Sigma-Aldrich). The next day ...
-
bioRxiv - Immunology 2022Quote: ... excess biotin was removed using Amicon Ultra 0.5L columns (3K, Millipore Sigma). The biotinylated whole-cell lysate was then added to FITC neutravidin beads (ThermoFisher ...
-
bioRxiv - Genomics 2022Quote: ... Biotin-labelled HSAT1 probe was detected with Streptavidin Cy3 conjugated (Sigma-Aldrich). Preparations were mounted using Vectashield containing 4’-6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Immunology 2022Quote: ... Expamer stimulus was disrupted by incubation with 1 mM D-biotin (Sigma). 1×106 cells were electroporated (pulse code EH100 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The Azide-647 was replaced with Azide-PEG3-Biotin (Sigma, 10 mM) and supplements with 6 mM THTPA (Jena Bioscience).
-
bioRxiv - Bioengineering 2023Quote: ... Membranes were probed overnight at 4°C with anti-HA antibody (MBL, M180-3) and anti-glyceraldehyde-3-phosphate dehydrogenase antibody (Sigma-Aldrich, G8795) or with anti-dystrophin (Sigma-Aldrich ...
-
bioRxiv - Biophysics 2022Quote: ... and mouse monoclonal primary antibody against myelin basic protein antibody (Millipore) at 1:200 dilution ...
-
bioRxiv - Cell Biology 2023Quote: ... Cenexin rabbit antibody (Protein tech) and centrin mouse antibody (EMD Millipore) diluted 1:1000 for centrin and 1:200 for other centrosomal proteins in 1% BSA in PBS for 1.5 hours at room temperature ...
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant (total protein concentration ∼3 mg/ml) was incubated with ANTI-FLAG M2 agarose beads (Sigma Aldrich) at 4°C for 1 h with agitation ...
-
bioRxiv - Biophysics 2021Quote: ... The protein solution was then concentrated using a 3 kDa cutoff centrifugal filter unit (Millipore, Burlington, MA, USA) and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences ...
-
bioRxiv - Neuroscience 2019Quote: ... Protein lysate was isolated in RIPA buffer supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich). Lysate (20 μg ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated to ∼500 μL using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore MWCO 3 kDa). The concentration of proteins was determined using JASCO V730 UV-vis spectrophotometer at 280 nm ...
-
bioRxiv - Plant Biology 2020Quote: ... antibodies and Protein A agarose beads (Sigma). After elution of immune complexes ...
-
bioRxiv - Cell Biology 2020Quote: ... Polyclonal rabbit-anti-Protein A antibody (Sigma) and monoclonal mouse-anti-TAC102 antibody were used as described above ...
-
bioRxiv - Genetics 2023Quote: ... Rabbit anti-Protein A antibody (P3775, Sigma) and the HRP conjugated Goat anti-Rabbit secondary antibody ...
-
bioRxiv - Immunology 2021Quote: ... #Janelia 2014-013) and biotin in the presence of 1 mM Biotin (Sigma-Aldrich, #B4501), 1 μM BirA and 10 mM ATP (Gold Biotech ...
-
bioRxiv - Bioengineering 2023Quote: ... we incubated tissue sample in 0.5mM biotin phenol solution (Biotin tyramide, Sigma Aldrich; SML-2135) for 15 minutes ...
-
bioRxiv - Genetics 2020Quote: ... Antibody bound DNA-proteins were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight while shaking at 4◦C in the blocking solution that contained combinations of the following primary antibodies: mouse anti-3 microtubule-binding domain tau protein (3R-Tau, 1:500, Millipore, Cat# 05-803), rabbit anti-Purkinje cell protein 4 (PCP4 ...
-
bioRxiv - Cell Biology 2021Quote: ... and 3 μL anti-Flag antibody M2 (Sigma; F3165). After binding ...
-
bioRxiv - Cell Biology 2021Quote: ... and 3 μL anti-Flag antibody M2 (F3165, Sigma). The beads were washed once with 1 mL lysis buffer ...
-
bioRxiv - Neuroscience 2023Quote: Tadpoles were anesthetized in buffered 0.01% MS-222 in frog water and given an intraperitoneal (ip) injection of a selective D1 family agonist (10ug/gbm; SKF 38393; Sigma, #D047) or antagonist (0.5ug/gbm ...
-
bioRxiv - Molecular Biology 2019Quote: ... Ten milligrams of protein was incubated with 4 μL α-myc antibody (Sigma M4439) for 1 hour at 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... Twelve milligrams of protein was incubated with 4 μL α-myc antibody (Sigma M4439) at 4°C overnight while rotating ...
-
bioRxiv - Neuroscience 2019Quote: ... The antibody was captured using 30 μl of 50% Protein G Sepharose beads (Sigma) for 2 h at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... ABCF1 protein was probed with primary anti-ABCF1 antibody (HPA017578, Sigma-Aldrich, Oakville, Ontario) at 1:100 in 3% Casein in 1X Tris Buffered Saline with TWEEN ® 20 (Sigma-Aldrich ...
-
bioRxiv - Immunology 2021Quote: Actin was used as a housekeeping protein (Rabbit anti-Actin antibody (Sigma-Aldrich A2066) followed by Goat anti-Rabbit-HRP (Southern Biotech 4050-05)).
-
bioRxiv - Plant Biology 2022Quote: ... Protein ubiquitination was tested by immunoblots and FLAG-HRP antibody (Sigma, Catalog number A8592).
-
bioRxiv - Microbiology 2023Quote: ... The DNA protein complex was immunoprecipitated with either anti-FLAG monoclonal antibody M2 (Sigma) or anti-σ70 antibody (BioLegend ...
-
bioRxiv - Biochemistry 2024Quote: ... The antibody-lysate complex was incubated with protein A- conjugated sepharose beads (Sigma #P3391) and eluted in 2X laemelli dye ...
-
bioRxiv - Microbiology 2022Quote: ... Cap2-3×FLAG was immunoprecipitated using magnetic beads covalently linked to the αFLAG M2 antibody (Sigma). Finally ...
-
bioRxiv - Developmental Biology 2023Quote: ... Blocking and antibody incubation was done using 3% bovine serum albumin (BSA) (Sigma-Aldrich A7906-10G) and 0.3% TritonX (Sigma-Aldrich T8787 ...
-
bioRxiv - Molecular Biology 2019Quote: ... and 3×FLAG::GFP-tagged protein and associated RNAs were eluted with 100 μg/mL 3×FLAG peptide (Sigma). The eluates were incubated with TRIzol reagent (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: Approximately 5×105 cells stably expressing the indicated TurboID-V5-fusion proteins were treated as described prior to the addition of 500μM biotin (Sigma-Aldrich) for 30min ...
-
bioRxiv - Neuroscience 2021Quote: ... Antibody bound DNA-protein complexes were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed and eluted ...
-
bioRxiv - Biophysics 2019Quote: ... All proteins were expressed in Rosetta (DE3) plyS cells (Novagen #70956-3) in an LB medium ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were concentrated to approximately 3 mg/ml by ultrafiltration (Millipore, UFC905096) at 4 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... Supernatants were then transferred to 3 kDa protein-filter columns (Sigma-Aldrich) and centrifuged at 12,000g for 45 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... resuspended cells were supplemented with Bug Buster protein reagent (Millipore #70584-3) before being sonicated ...
-
bioRxiv - Developmental Biology 2022Quote: ... The reaction was developed using 3-3’-Diaminobenzidine (DAB) (Sigma). For CLASP1 immunostaining ...
-
bioRxiv - Cancer Biology 2019Quote: ... 3-hydroxybutyrate (3-OHB) was also purchased from Sigma (#166898).
-
bioRxiv - Neuroscience 2024Quote: Detection of newly synthesized proteins by proximity ligation assay was carried out using anti-puromycin antibody in combination with protein-specific antibodies and detection using Duolink reagents (Sigma) according to the manufacturer’s recommendations ...
-
bioRxiv - Biophysics 2020Quote: ... ATTO 565-biotin (92637, Sigma-Aldrich) was dissolved in DMSO to a concentration of 0.4 mM ...
-
bioRxiv - Neuroscience 2020Quote: ... 50 µM of biotin (Sigma-Aldrich) was simultaneously added to the culture media ...
-
bioRxiv - Bioengineering 2021Quote: ... 10 μg/ml biotin (Sigma, B4639), 30 ng/ml β-estradiol (Sigma ...