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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 33 mM biotin (Sigma), and 17 mM D-pantothenate (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin (Sigma, #B4501-100MG) was stored as a powder at room temperature and solubilized in serum-free DMEM to the indicated concentration immediately before use.
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bioRxiv - Cell Biology 2024Quote: ... 50 μΜ biotin (Sigma) and 1 μg/mL doxycycline in culture medium for a further 9 hours ...
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bioRxiv - Neuroscience 2023Quote: ... GluR2/3: Anti-Glutamate Receptor 2 & 3 antibody (1:500, Millipore, Cat# AB1506). c-Fos ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...
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bioRxiv - Biochemistry 2020Quote: ... The purified protein 5-HT2C or HCA2 (3 μg) was immobilized on nickel agarose beads (Sigma) in the incubation buffer containing 50 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... Expression of the proteins of interest was induced by 3 μg/ml doxycycline (dox, Sigma-Aldrich) added to the culture medium ...
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bioRxiv - Cell Biology 2023Quote: ... TEV-eluted supernatant was added to 100 µL of S-protein agarose (69704-3, EMD Millipore) to capture S-tagged protein ...
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bioRxiv - Cancer Biology 2023Quote: ... Channels were treated with anti-biotin antibody solution (Sigma, B3640, 1 mg/ml in PBS) after 5 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... The chambers were incubated with 20 µg/mL anti-biotin antibodies (in PBS, #B3640, Sigma) for 5 min or 20 µg/mL anti-β-tubulin antibodies (in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The flow cells were incubated with 20 µg/ml anti-biotin antibody (Sigma Aldrich, B3640) in PBS for 10 min and passivated by 1% Pluronic F-127 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Protein-antibody binding was visualized using Immobilon Forte Western HRP Substrate (Millipore) on the LAS-3000 imaging system (FUJIFILM ...
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bioRxiv - Genetics 2024Quote: ... Captured protein was detected by immunoblotting with anti-FLAG M2 antibody (Sigma) and visualized using an Odyssey Infrared Imaging System (Licor) ...
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bioRxiv - Microbiology 2020Quote: ... Membrane was immunoblotted for ≥ 3 h with primary antibodies : anti-M2 (1:5,000) (Sigma), anti-CtrA (1:5,000) ...
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bioRxiv - Developmental Biology 2022Quote: ... Non-specific antibody binding was minimised by incubating cells with 3 % BSA (Sigma-Aldrich) + 0.1 % Triton X-100/PBS for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation was performed by adding 3 µg of HA antibody (monoclonal 12CA5; Millipore Sigma) to the remaining extract and incubating at 4°C for 4 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of protein lysate were incubated for 3 h with 2 μg of α-FLAG M2® antibody (Sigma-Aldrich) pre-coupled to 20 μl of rotein G Dynabeads® (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with S9.6 antibodies (8 μ antibody/ 5μ DNA) overnight at 4°C followed by incubation for 3 hours with protein G-Sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Bioengineering 2021Quote: ... Equal amounts of protein were subject to SDS-PAGE and blotted with anti-Rho antibody (3 μg/mL, #05-778, EMD Millipore) or anti-GAPDH antibody (1:1000 dilution ...
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bioRxiv - Biophysics 2019Quote: ... then bound proteins were eluted with 3 mM desthiobiotin (Sigma) or 50 mM D-biotin (CHEM-IMPEX ...
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bioRxiv - Cell Biology 2023Quote: ... the FSH-cultured follicles were then incubated with or without the PPP family phosphatase inhibitor cantharidin (C7632, Sigma-Aldrich) or the PKA inhibitor Rp-8-CPT- cAMPS (C011-05 ...
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bioRxiv - Cell Biology 2022Quote: ... and addition of biotin supplemented media (3mM of D-biotin, B4501 Sigma-Aldrich), by using a magnetic imaging chamber with L-shape tubing (CM-B25-1PB ...
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bioRxiv - Biochemistry 2021Quote: ... Casein-based blocking solution was used for anti-biotin blots (Sigma). Primary antibodies were incubated over-night at 4°C and secondary antibodies 1 hour at room temperature (RT) ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotin stripping was achieved by incubation with 100 mM MESNA (Sigma) for 75 minutes at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Elution was then added with 250U biotin labeled thrombin (EMD Millipore) and dialyzed in 3,350 MW dialysis tubing o/n in 1L Thrombin Dialysis Buffer (Thrombin Dialysis Buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... medium was supplemented with 50 µM exogenous biotin (Sigma-Aldrich; #B4501) 10 min prior to IL-1α treatment (10 ng/ml ...
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bioRxiv - Cell Biology 2021Quote: ... 3 µg antibody against Flag (Sigma-Aldrich, #F1804), H3K27ac (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... Par-3 antibody (Millipore, 07-330, 1:200), ZO-1 antibody (Life Technology ...
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bioRxiv - Plant Biology 2022Quote: ... About 3 μL anti-GFP antibody (Sigma-Aldrich) was added to incubation buffer and rotated at 4℃ for 2 h ...
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bioRxiv - Neuroscience 2021Quote: ... histone 3 lysine 27 tri-methylation (H3K27me3) and histone 3 antibodies (all from Millipore). Positive bands were detected by chemiluminescent reagents (Thermofisher ...
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bioRxiv - Cell Biology 2020Quote: ... 1-5ug of antibody was incubated overnight with chromatin and the complex antibody-chromatin was then captured with G protein sepharose beads (Sigma Aldrich) 1h at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... 1-oleoyl-2-(12-biotinyl(aminododecanoyl))-sn-glycero-3-phosphoethanolamine (biotin-PE) and Tween-20 were purchased from Sigma Aldrich. Tween-20 was suspended in 50 mM Tris buffer (pH 8 ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed 3 times for 15 min in PBS and incubated in 0.0001% biotin-tyramide (Sigma Aldrich, SML2135-50MG) and 0.003 % H2O2 in 0.05 M borate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Azo biotin-azide (CAS No. 1339202-33-3) and Imatinib (Cas No.152459-95-5) were obtained from Sigma Aldrich, rapamycin (Cas No.53123-88-9 ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 mg of protein from each sample were incubated with 3 ug of HOXA9 (Millipore # 07-178) or Rabbit IgG Isotype control (Invitrogen # 02-6102 ...
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bioRxiv - Cancer Biology 2021Quote: ... PreScission protease-eluted supernatant was added to 100 mL of S-protein agarose (EMD Millipore, 69704-3) to capture S-tagged protein ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... was washed 3 times with PBS and processed using the CelLytic MEM protein extraction kit from Sigma. Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Protein degradation rate was measured after 3-hour treatment with 20μg/ml of cycloheximide (Sigma-Aldrich #C7698) in the complete RPMI medium ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to approximately 3 mg/ml using an ultrafiltration pressure cell (Millipore, Billerica, MA) with a 10-kDa molecular mass cutoff membrane and buffer exchanged with 20 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein elution was carried out under native conditions by competition with commercially available 3× FLAG peptide (Sigma). Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... Exposure to primary antibodies (rat - anti GFP 3H9-Proteintech or mouse – anti Biotin B7653-Sigma-Aldrich) was done for 2 hours at 25°C ...
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bioRxiv - Plant Biology 2021Quote: ... and immunoprecipitation of the antibody/protein complexes was performed by adding 50 μl of Protein A-Sepharose (Sigma-Aldrich) made up as a 50% (w/v ...
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bioRxiv - Cell Biology 2020Quote: Protein loading quantity was controlled using a monoclonal anti-α-tubulin antibody (Sigma). Secondary antibodies were from Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... 10μg of antibody was coupled to 50μl packed Protein A beads (Sigma P9424) by incubation in 1ml PBS (0.1% Tween-20 ...
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Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2bioRxiv - Molecular Biology 2019Quote: ... and Spo11 was immunoprecipitated from total soluble protein using anti-FLAG antibody (Sigma) and protein-G-agarose (Roche) ...
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bioRxiv - Biochemistry 2020Quote: ... ATP11A protein was labeled with mouse anti-Flag antibody (1:2000, Sigma, Germany) and the endoplasmic reticulum (ER ...
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bioRxiv - Biochemistry 2020Quote: ... Tie2 protein was immunoprecipitated from the lysates by using anti‐Tie2 antibody (Millipore) and protein A/G‐sepharose ...
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bioRxiv - Cell Biology 2021Quote: ... Protein loading was controlled by Vinculin (VCL) antibody (1:5000, V3131, Sigma-Aldrich). Horseradish peroxidase conjugated goat anti-mouse IgG antibody (1:10000 ...