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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... and immunoprecipitated proteins were eluted 3 times with 150µL 3x-FLAG peptides (Sigma, F4799) for 25 min each at 25°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Not protein depletions were obtained by addition of auxin (3-indoleacetic acid, Sigma-Aldrich I2886 ...
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bioRxiv - Immunology 2022Quote: ... Proteins were reduced with the addition of 3 μL of 250 mM dithiothreitol (Sigma) at 37°C for 45 min and alkylated with 4.5 μL of 500 mM chloroacetamide for 30 min at room temperature in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... growth media was replaced with complete growth media supplemented with 50 μM biotin (Sigma-Aldrich) and cultured for a further for 16 hours ...
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bioRxiv - Biophysics 2020Quote: ... The pellet was then resuspended in conjugation buffer containing 1 mM Biotin-NHS (Sigma 203118) by pipetting up and down ...
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bioRxiv - Bioengineering 2022Quote: ... Excess biotin was removed with a 5K MWCO concentrator (CLS431487-12EA, Millipore Sigma, MA, USA) at 4000xg for 100 minutes at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... The eluted His-tagged RBD was then incubated with biotin-tagged HRV C3 protease (Sigma) and passed through a streptavidin-agarose column to deplete the protease and the 8XHis-SBP peptide ...
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bioRxiv - Cell Biology 2020Quote: ... Growth media was replaced with complete growth media supplemented with 50 μM biotin (Sigma-Aldrich), and cells cultured for a further 16 hours ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... the incubation was conducted with anti-rabbit biotin (1:300, B8895, Sigma-Aldrich, Darmstadt, Germany) followed by incubation with streptavidin-Alexa 488 (1:600 ...
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bioRxiv - Cancer Biology 2023Quote: ... Click chemistry reaction was performed with biotin-PEG4-alkyne (100 μM, Cat# 764213, Millipore Sigma), CuSO4 (1 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblotting for specific proteins was carried out using the following primary antibodies: Mouse monoclonal antibodies against anti-FLAG M2 (Millipore Sigma, Billerica, MA), Jak1 (CST-50996) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851 ...
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bioRxiv - Genetics 2020Quote: ... Protein– antibody interaction was detected with either anti-mouse or anti-rabbit peroxidase-conjugated mouse IgG antibody (Sigma-Aldrich, St Louis, MO, USA), using Amersham™ ECL Plus western blotting detection system (GE Healthcare Life Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with an α-FLAG antibody (Sigma; 1:1,000 in 1x TBS-T containing 3% BSA) at 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... The coverslip was rinsed 3 times with PBS-T and incubated with Alexa Flour conjugated secondary antibodies (Sigma-Aldrich) and Hoechst 33342 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... antibody overnight and then with equilibrated protein G beads (Millipore Sigma, Protein G Agarose, Fast Flow) for 2h ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... This magnetic resin was generated by incubating Dynabead Protein G (ThermoScientific) with anti-FLAG antibodies (Sigma Aldrich) at room temperature followed by washing ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg fibrillarin 2 or NOP56 antibody was coupled to 30 μL protein-A agarose beads (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Total protein synthesis was measured by immunoblot using the anti-puromycin antibody at 1:500 (EMD Millipore). Protein synthesis was quantified by densitometry using ImageJ and normalized by immunoblot using antibody specific to steady-state levels of eIF2α protein (13).
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bioRxiv - Neuroscience 2020Quote: ... Antibody-bound chromatin complex was precipitated with salmon sperm DNA/Protein A agarose (EMD Millipore, Burlington, MA), then washed sequentially with PIRA (150 mM NaCl ...
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bioRxiv - Bioengineering 2019Quote: RanBP9 was immunoprecipitated from total protein extracts using monoclonal αHA-Agarose antibody (Sigma cat. nr. A2095-1ML). Briefly ...
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bioRxiv - Physiology 2021Quote: ... Protein was detected using a secondary HRP-linked antibody and Luminata Classico Western HRP Substrate (Millipore WBLUC0020). To reprobe membranes ...
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bioRxiv - Genetics 2020Quote: ... Equal protein loading was confirmed by probing with mouse-anti-β-actin antibody (1:10,000; Sigma, A5441).
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bioRxiv - Microbiology 2021Quote: ... This magnetic resin was generated by incubating Dynabead Protein G (ThermoScientific) with anti-FLAG antibodies (Sigma Aldrich) at room temperature followed by washing ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection of proteins was performed by using primary rabbit anti-human hepsin antibody (Sigma Aldrich, Steinheim, Germany), pAKT (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Immunodetection of ProteinA (ProtA)-tagged proteins was performed using Peroxidase Anti-Peroxidase Soluble Complex antibody (Sigma-Aldrich) in a 1:3,000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... total protein was extracted and examined via western blots using HRP-conjugated anti-mouse secondary antibody (Sigma). Protein loading was visualized with Amido Black Staining Solution (Sigma).
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bioRxiv - Microbiology 2024Quote: ... was used as second antibody and proteins on the membrane were visualized using BCIP/NBT substrate (Sigma). When deer plasma specimens were used as primary antibody ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each mouse was intraperitoneal injected with 3 mL of 3% thioglycolate (Sigma-Aldrich) on day 1 and sacrificed using isoflurane on Day 3 ...
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bioRxiv - Cancer Biology 2023Quote: Butyrolactone-3 (MB-3) was purchased from Sigma-Aldrich (St Louis, MO, USA) and used at a 100µM concentration ...
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bioRxiv - Biochemistry 2022Quote: hnRNPDL proteins were detected using different primary antibodies (anti-hnRNPDL antibody, HPA056820 from Sigma-Aldrich or with an anti-GFP monoclonal antibody ...
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bioRxiv - Cell Biology 2020Quote: ... biotin (50 µM in H2O, Sigma-Aldrich, B4501), Z-VAD-FMK (50 or 100 µM in DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... 150ml of PBS containing 6mM free biotin (Sigma) was added to the beads ...
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bioRxiv - Neuroscience 2020Quote: ... Biotin (Sigma-Aldrich, MO, US; Cat. No. #B4501) 100 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5 mM biotin (Sigma-Aldrich, St Louis, MI), 5% (v:v ...
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bioRxiv - Biochemistry 2020Quote: ... Dibenzocyclooctyne-PEG4-biotin conjugate (#760749-5MG, Sigma-Aldrich) was used without further purification ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 100 µM D-biotin (Sigma #2031). Human proteins were incubated for 120 hours at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... GAGs were reacted with sulfo-DBCO-biotin (Sigma) (6.7 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM biotin picolyl azide (Sigma-Aldrich 900912), 10 mM sodium ascorbate made fresh (Sigma 11140) ...
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bioRxiv - Cell Biology 2022Quote: ... Biotin labeled bovine albumin (Sigma-Aldrich A8549-10MG).
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bioRxiv - Neuroscience 2022Quote: ... 1 μM D-biotin (Sigma-Aldrich, Cat# B4501), 1% BSA fatty acid-free (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... but additionally incubated with 50 μM biotin (Sigma) for 24 h before harvesting ...
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bioRxiv - Molecular Biology 2021Quote: ... We then added 1 mM biotin (B4501, Sigma) to neutralize the streptavidin molecules not bound to HELB ...
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bioRxiv - Cell Biology 2019Quote: ... 5 mg/ml Biotin-BSA (A8549; Sigma-Aldrich) for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... 5 mg/ml Biotin-BSA (A8549; Sigma-Aldrich) for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... or Azide-PEG3-biotin (Sigma-Aldrich, Cat#: 762024) (2 eq. ...
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bioRxiv - Immunology 2021Quote: ... 100 mM D-biotin (Sigma, cat. no. B4639) was added immediately post-transfection to a final concentration of 10 mM ...
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bioRxiv - Microbiology 2022Quote: ... 0.2mM Biotin-16-UTP (Sigma-Aldrich, REF:11388908910) and 0.3mM unlabeled-UTP were added to the reaction mixture to produce biotinylated RNA ...