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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Cholesteryl hemisuccinate (CHS) and tobacco etch virus (TEV) protease were from Sigma-Aldrich.
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bioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated and purified via Iodixanol gradients (“Optiprep” Sigma D1556-250mL). Centrifuge the gradients for 90 min at 46,500 rpm at 16 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... virus containing supernatant was harvested and filtered (0.45 μm, Millex-HV Filter; Millipore). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were infected with virus in the presence of 8μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... Live filtered virus was used to transduce cells with polybrene (10μg/ml, Sigma).
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bioRxiv - Cell Biology 2020Quote: ... the virus was concentrated using Amicon ultra-15 centrifugal filter units (Millipore Sigma) by centrifuging at 3000 rpm and stored at -80°C until use ...
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bioRxiv - Immunology 2019Quote: ... Virus-containing supernatants were filtered through a 0.45-μm-pore-size filter (Millipore) and supplemented with polybrene (8 μg/ ml ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Microbiology 2019Quote: ... virus dilutions were overlaid with 1 ml of Opti-MEM [1% Carboxymethylcellulose (Sigma), 2% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Virus inoculum was removed and cells overlaid with MEM containing 1% carboxymethylcellulose (Sigma), 4% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... 100 μl of virus preparations were treated with 25 units of Benzonase (Sigma) for 30 min before RNA isolation.
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bioRxiv - Cancer Biology 2020Quote: ... the virus was harvested from the medium filtered by 0.45 mm Steriflipfilter (Millipore). Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma ...
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bioRxiv - Microbiology 2020Quote: Cells or virus were lysed with RIPA buffer and protease inhibitor cocktail (Sigma). Protein loaded from viral lysates were normalized to p24 antigen content ...
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bioRxiv - Immunology 2021Quote: ... Virus and cell pellets were solubilized in lysis buffer [10mM iodoacetamide (Sigma-Aldrich), CompleteTM protease inhibitor tablets (Roche) ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was aspirated and cells resuspended in virus medium containing TPCK-trypsin (Sigma), 20 mM HEPES (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Neuroscience 2023Quote: ... The virus was slowly mixed with silk fibroin solution (50mg/mL, Millipore Sigma) with a ratio of 1:1 through the pipette to avoid generating bubbles ...
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bioRxiv - Immunology 2023Quote: ... virus particles were precipitated by adding 50% (w/v) PEG 6000 (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus containing medium with 8 μg/mL polybrene (Sigma-Aldrich, Cat #H9268) was used in 1:1 dilution with the culture medium to infect SKBR3 and HCC1954 cells ...
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bioRxiv - Neuroscience 2023Quote: ... the virus-containing medium was filtered through Costar Spin-X columns (Sigma #8162) as previously described 31.
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bioRxiv - Bioengineering 2023Quote: ... and Anti-Adeno-associated Virus 9 Antibody clone HL2374 (Millipore Sigma, #MABF2326-25UG). Following three additional washes ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2023Quote: ... Virus-containing medium was passed through a 0.45 µm filter (cat. #SLHVR33RB, Millipore), aliquoted and stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... The virus-containing supernatant was filtered through 0.45-mm polyvinylidene difluoride filters (Millipore) and precipitated in 8.5% polyethylene glycol [average molecular weight (Mn) ...
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bioRxiv - Microbiology 2024Quote: ... The virus-containing supernatant was filtered through 0.45-mm polyvinylidene difluoride filters (Millipore) and precipitated in 8.5% polyethylene glycol [average molecular weight (Mn) ...
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bioRxiv - Cancer Biology 2021Quote: ... and bounded proteins were eluted with NETN buffer containing 2 mg/ml biotin (Sigma-Aldrich) for 1 h twice at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The bacterial pellet was lysed in 2 mL 1× BugBuster™ protein extraction reagent (Novagen), incubated by slow shaking for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins denatured using SDS/PAGE loading buffer (bio rad) and 2-mercaptoethanol (Sigma-Aldrich). Proteins were separated by gel electrophoresis (NW04120BOX ...
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bioRxiv - Biochemistry 2022Quote: WT and mutant Streptococcus pyogenes Cas9 proteins were expressed in E.coli Rosetta 2 (DE3) (Novagen) for 16 hours at 18 °C as fusion proteins with an N-terminal His6-MBP-TEV tag ...
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bioRxiv - Pathology 2022Quote: ... 15-30 μg protein per sample were denatured with 5 % vol/vol 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in Bolt™ 4–12 % Bis-Tris gels (Thermo Fisher) ...
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bioRxiv - Biophysics 2022Quote: ... The purified protein was then reduced with tris(2-carboxyethyl)phosphine (TCEP, obtained from Sigma), a strong ...
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bioRxiv - Neuroscience 2019Quote: ... monoclonal anti-mouse microtubule-associated protein 2 (MAP2; Millipore Sigma, Burlington, MA; 1:1000 dilution), and monoclonal anti-mouse primary conjugated-cy3 glial fibrillary acidic protein (GFAP ...
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bioRxiv - Immunology 2019Quote: ... Protein aliquots were reduced with 5 mM tris-2-carboxymethyl phosphine (TCEP) buffer (Sigma Aldrich) and incubated for 1 hour at 60°C to reduce disulfide bonds ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mgs of total protein were incubated with 20 μL of V5 agarose (Sigma-Aldrich) rotating at 4 °C for 2 hr ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue lysates were precleared for 2 h with Protein A + G magnetic beads (EMD Millipore). Precleared lysates were then incubated with 5 μg of anti-Sox9 antibody (Abcam ...
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bioRxiv - Biophysics 2019Quote: ... GFP-PRC1 isoform 2 proteins were expressed via BL21(DE3) Rosetta Escherichia coli cells (Novagen). Full length PRC1 was expressed for 4 hours at 18 °C after induction with 0.5mM IPTG ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was degraded by adding 2 µL 18.2 mg/mL Proteinase K (Millipore Sigma 3115887001) and incubating at 55°C for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... and proteins denatured using SDS/PAGE loading buffer (bio rad) and 2-mercaptoethanol (Sigma-Aldrich). Proteins were separated by gel electrophoresis (NW04120BOX ...
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bioRxiv - Systems Biology 2022Quote: Single-cell protein lysates were digested with 2 ng of Trypsin (Sigma cat. nr. T6567) supplied in 1 μL of digestion buffer (100 mM TEAB pH 8.5 ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were expressed in Escherichia coli strain BL21(DE3) or Rosetta 2 (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Physiology 2023Quote: ... 15-35 µg protein per sample were denatured with 5 % v/v 2-mercaptoethanol (Sigma) in LDS sample buffer (Thermo ...
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bioRxiv - Neuroscience 2023Quote: Total protein samples were lysed in radioimmunoprecipitation (RIPA) buffer containing 2% protease inhibitor cocktail (Sigma). Protein quantification was completed using the Pierce Bicinchoninic acid (BCA ...
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bioRxiv - Biochemistry 2023Quote: ... TEV digested protein was concentrated to < 2 mL using an Amicon Ultra-15 concentrator (Millipore) and then purified by size exclusion chromatography ...
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bioRxiv - Neuroscience 2023Quote: ... beads were incubated directly with 4X protein loading buffer (Li-Cor) with 2-Mercaptoethanol (Sigma). For digital PCR ...
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bioRxiv - Immunology 2023Quote: Recombinant SARS-CoV-2 BA.1 nucleocapsid protein was subcloned into the pET46 vector (Novagen) with an upstream 6xHis tag and expressed in Rosetta2 pLysS E ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... HEK293T cells were co-transfected with library plasmids and suitable packaging- and envelope plasmids (CRISPR & MISSION® Lentiviral packaging mix, Sigma-Aldrich) by using Lipofectamine 2000 Transfection Reagent according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: For immunofluorescence experiments the panel of HEK293 cells grown on poly-Lysine (Sigma-Aldrich Cat. Nr. P6282) pre-coated (2.5 μg/mL in MQ H2O 30 min at room temperature (RT) ...