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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 167 mM NaCl) and precleared for 2 h with 250 μL of previously blocked 50% protein-A and protein-G beads (Sigma P-7786 and P-3296 respectively). Blocking was achieved by incubating the beads with 0.5 mg/ml of Ultrapure BSA and 0.2 mg/mL of salmon sperm DNA for 4 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... For p65 IP the lysates were then incubated with 2 μg of antibody CST ref #6956 or CST ref# 8242) at 4 °C for 2 hrs before adding 20 μL of slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose) for 20 min ...
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bioRxiv - Microbiology 2021Quote: ... The filtered virus was mixed with fresh media (30% vol/vol) that included polybrene (Sigma) at a 6 μg/ml final concentration ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µL of virus was added per well with 10 µg/mL of polybrene (Sigma) in 100 µL of EMEM without antibiotics or serum overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer extension was conducted with 4 units of Avian Myeloblastosis Virus (AMV) reverse transcriptase (Sigma) for 1 hour at 15°C ...
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bioRxiv - Cancer Biology 2019Quote: ... concentrated virus was added to the cells along with 8 ng/mL polybrene (Sigma-Aldrich) and the cells were incubated at 37°C plus 5% CO2 in humidified air ...
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bioRxiv - Cancer Biology 2021Quote: ... virus was washed out and replaced with fresh complete medium with 10μg/ml blasticidin (Sigma). After 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mL of freshly thawed virus soup was mixed with polybrene (Cat# H9268, Sigma-Aldrich) to a final concentration of 8 μg/mL and used to infect A549N and HT-1080N cell lines ...
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bioRxiv - Microbiology 2020Quote: Virus titres were quantified by plaque assay in 12-well plates (Millipore Sigma, Cat# Z707775). For the quantification of FCV ...
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bioRxiv - Neuroscience 2019Quote: ... The virus was subsequently concentrated by mixing the filtered supernatant with 50% PEG-8000 (Sigma) in a ratio of 4:1 and placed at 4°C over night ...
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bioRxiv - Immunology 2020Quote: ... sucrose gradient-purified AD169r-GFP virus was transferred to a 100,000 kDa Amicon filter (Millipore), then buffer exchanged with 1× PBS ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The virus particles were then concentrated to 300µl using Amicon Ultra Centrifugal Filters (UFC910024 SIGMA) by spinning at 1000g at 4°C for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... harvested virus was added onto target cells in the presence of 8μg/ml polybrene (Millipore). For in vivo experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were infected with virus-containing media using Polybrene reagent (TR-1003-G; EMD Millipore) according to the manufacturer’s instructions and selected for 48 hr in 2 μg/mL of puromycin.
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bioRxiv - Immunology 2020Quote: ... We generated HAdV-lactoferrin complexes by incubating the virus with 40 μg lactoferrin (Sigma-Aldrich) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Virus-containing supernatant was concentrated using Amicon Ultra-15 100kDa MWCO centrifugal filters (Millipore, UFC910024). For siRNA knockdown experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Undiluted virus was added dropwise to cells with 10 μg/mL polybrene (Sigma-Aldrich; TR1003G), and incubated at 37°C for 24 hrs ...
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with .3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free MEM supplemented with 3% BSA (Sigma), 100U/mL of penicillin and 100ug/mL of streptomycin mixture (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... virus stocks were concentrated on a 100 KDa cellulose membrane (Amicon Ultra-15, Merck Millipore) and rinsed three times successively with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... virus at the MOI of 50 was applied with 8 μg/ml Polybrene (Merck Millipore). Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... the virus-containing supernatant was filtered through a 0.45-μm pore size PVDF filter (Millipore), and lentiviral particles were concentrated using the Lenti-X Concentrator (Takara ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Physiology 2019Quote: ... Proteins were resuspended in concentrated urea buffer (8 M Urea, 2 M Thiourea, 150 mM NaCl (Sigma)) ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were eluted twice with 2 ug/uL V5 peptide in water (Sigma-Aldrich, St. Louis, MO) at 37°C for 15 minutes with shaking at 1000rpm ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg fibrillarin 2 or NOP56 antibody was coupled to 30 μL protein-A agarose beads (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl of the supernatant were used for protein quantification using the Direct Detect system (Merck Millipore).
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Cancer Biology 2019Quote: ... The lyophilized proteins were resuspended in 1 ml of a 2 mg/ml DTT (1,4-Dithiothreitol, Sigma) solution and incubated at 50C for 2h ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 mg total protein were mixed with 2 mL Urea Buffer (UB) (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.0 and 50mM ammonium bicarbonate) ...
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bioRxiv - Neuroscience 2019Quote: ... Eya1 protein was concentrated to approximately 2 mg/ml using an Amicon Ultra concentrator (30 MWCO, Millipore) and further purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Lifescience ...
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bioRxiv - Biochemistry 2021Quote: ... protein lysates from the single cells were digested with 2 ng of Trypsin (Sigma cat. nr. T6567), dissolved in 1 μl of 100 mM TEAB pH 8.5 containing Benzonase (Sigma cat ...
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bioRxiv - Immunology 2020Quote: ... Detection of the SARS CoV-2 spike protein was achieved by incubation with an anti-rabbit (Sigma) secondary antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... (Table 2) and the immune complexes were captured with protein A agarose fast-flow beads (Sigma-Aldrich) for 1h at RT ...
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bioRxiv - Systems Biology 2021Quote: ... protein lysates from the single cells were digested with 2 ng of Trypsin (Sigma cat. nr. T6567), dissolved in 1 μl of 100mM TEAB pH 8.5 containing Benzonase (Sigma cat ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was reduced with a final concentration of 10 mM Tris(2 - carboxyethyl)phosphine hydrochloride (TCEP) (Sigma) and alkylated with 40 mM 2-chloroacetamide (CAA ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were eluted by 2 mM biotin in buffer W and concentrated with 100kDa Amicon (Millipore). The concentration of dtSARM1 was determined by BCA assay (Thermo Fisher).
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bioRxiv - Plant Biology 2023Quote: ... Labelled proteins were biotinylated with 20 μM azide-PEG3-biotin (Sigma-Aldrich, 2 mM stock in DMSO) using click chemistry as described in “Labeling and click chemistry” ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... 2 mgs of total protein were incubated with 20 μL of V5 agarose (Sigma-Aldrich, Catalog # 7345) or FLAG M2 resin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... total proteins were loaded in the Laemmli sample buffer supplemented with 5% 2-mercaptoethanol (Sigma, catalog #: M3148) to reduce the disulfide bonds ...
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bioRxiv - Plant Biology 2024Quote: ... Labelled proteins were biotinylated with 30 μM azide-PEG3-biotin (Sigma-Aldrich, 2 mM stock in DMSO) using click chemistry as described above ...
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bioRxiv - Biophysics 2021Quote: The cAMP synthesis of heterologously expressed sAC in HEK293 lysates was induced by applying di-sodium-ATP (Sigma Aldrich) and MgCl2 or MnCl2 in isotonic HEPES buffer ...
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bioRxiv - Biochemistry 2020Quote: ... control and SENP3 over-expressed HEK293 cells were stimulated by poly (I:C) for 6h and lysed in NETN buffer supplemented with 10mM PMSF (Sigma). The whole cell lysates were immunoprecipitated with 10 μl Anti-Flag (M2)-agarose (Sigma ...
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bioRxiv - Microbiology 2020Quote: The human embryonic kidney cell lines HEK293 and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (D1152, Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells transfected and grown overnight prior to incubation with 5μg/ml cycloheximide (CHX; Sigma-Aldrich, St. Louis, MO) in the presence and absence of 15μM CQ (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... murine fibroblast (3T3, laboratory stocks) and HEK293 (laboratory stocks) cell lines were maintained in Dulbecco’s minimal essential medium (DMEM, Sigma) supplemented with 10% (v/v ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) and K562 cells in RPMI-1640 medium (Sigma-Aldrich). All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... were produced in HEK293 cells by mixing lentiviral vector and packaging vectors using branched polyethyleneimine (bPEI) (MW 25Kda, Sigma) as the transfection reagent ...