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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Molecular Biology 2021Quote: Retroviral infection was conducted in 6-well plates using either 1 mL of unconcentrated virus or 200 μL of concentrated virus in 20 mM HEPES pH 7.4 and 8 μg/mL polybrene (Sigma-Aldrich). If selection was desired ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma) for 8-12 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... One group was injected intraperitoneally (i.p.) with LPS (Escherichia coli serotype 0111:B4; Sigma-Aldrich Canada, Oakville, ON, Canada) on GD13.5 (10 µg/kg ...
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bioRxiv - Immunology 2022Quote: Pulmonary vasculitis was induced by intratracheal (i.t.) application of 50 µl PBS containing 10 µg LPS (Serotype 026:B6, Sigma) and 10 µg fMLP (Sigma ...
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bioRxiv - Immunology 2019Quote: ... 30 minutes before being challenged with 40 mg kg−1 LPS (E. coli, serotype 0111:B4, L-2630, Sigma). Mice were euthanized 3 hours after LPS challenge for blood collection ...
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bioRxiv - Molecular Biology 2020Quote: ... were treated with 1 μg/ml LPS from Escherichia coli (serotype 0111: B4, Sigma-Aldrich, St. Louis, MO, USA) and 100 ng/ml IFN-γ (ProSpec-Tany Technogene Ltd ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... RW and SED groups received an intraperitoneal injection (volume 10 mL/kg) of either LPS 0.33 mg/kg (serotype 0127: B8, Sigma) to induce neuroinflammation [7 ...
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bioRxiv - Immunology 2021Quote: ... LPS (Escherichia coli serotype 0111: B4) and Acid phosphatase leukocyte (TRAP) kit (387A) were procured from Sigma-Aldrich (USA). Macrophage-colony stimulating factor (M-CSF ...
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bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
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bioRxiv - Neuroscience 2020Quote: Virus was produced by polyethylenimine (PEI; Sigma-Aldrich) transfection of 89-90% confluent HEK293T cells with the viral vector together with the lentiviral packaging plasmids ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... or an empty vector control virus (Sigma SHC001V) followed by puromycin (1 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich) supplemented with 10 % fetal calf serum at 37°C/5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich) supplemented with 10% fetal calf serum at 37°C/5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293 suspension cells were transfected with constructs using polyethylenimine (PEI, Sigma). HEK293 suspension cells were cultured at 37°C and 8% CO2 and 120 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 and HeLa cells were cultured in DMEM media (Sigma, RNBK7590) supplemented with 10% free fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: HEK293 (CRL-1573-ATCC) were grown in DMEM (Sigma-Aldrich, USA) supplemented with 10 % FBS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells were seeded on poly-L-lysine (Sigma, Cat. #P8920) coated coverslips in 12-well plates ...
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bioRxiv - Biochemistry 2023Quote: HEK293 cells were maintained in DMEM F-12 Ham medium (Sigma) supplemented with 10% fetal bovine serum (GIBCO ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and HeLa cells were transfected with Polyethyleneimine (PEI, Sigma Aldrich) as previously described (Braun et al. ...
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bioRxiv - Cell Biology 2024Quote: HEK293 (Human Embryonic Kidney, ECACC 96121229 from Sigma-Aldrich Chemie GmbH) were cultured in DMEM (Dulbecco’s modified Eagle’s medium PAN biotech ...
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bioRxiv - Cancer Biology 2021Quote: ... To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0) and 5 μg / mL Polybrene infection reaction (EMD Millipore, TR-1003). For pLKO.1 vectors ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µg of Rab protein were mixed with 5x SYPRO® orange protein gel stain (Sigma) in a volume of 20 µl ...
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bioRxiv - Biochemistry 2021Quote: ... programed for uniform mixing as previously described at a controlled temperature and modified with online automated desalting.13 Proteins were denatured in a solution of 35% v/v 2-2-2 Trifluoroethanol (TFE, Sigma), 40 mM Dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were alkylated using 2-chloroacetamide (C0267-100G, Sigma-Aldrich) in elution buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-microtubule-associated protein 2 (Map2, 1:250, Sigma), rabbit anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Molecular Biology 2020Quote: ... containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma-Aldrich) and protease inhibitors (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2019Quote: ... 20μg protein was incubated in 10% 2-Mercaptoethanol (Sigma-Aldrich) in laemmli loading buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... protein expression was induced by 2 μg/mL doxycycline (SIGMA) for 48 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were reduced with tris-2-carboxyethyl phosphine (ΤCEP, Sigma) for 1h at 60°C at a final concentration of 5mM ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 μM M3814 (DNA-dependent protein kinase inhibitor) (Sigma-Aldrich) was added to the cell media for 72 hr to repress non-homologous end joining DNA repair (Riesenberg et al. ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-microtubule associated protein-2 (MAP2) (Millipore Sigma AB5622), and mouse anti-TUBB3/β3 tubulin (Novus Biologicals ...
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bioRxiv - Immunology 2023Quote: ... bound proteins were eluted in 2× Laemmli buffer (Sigma-Aldrich), boiled for 5 min at 95°C and separated on a 4-12% NuPAGE Novex Bis-Tris precast gel (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... The protein was expressed E.coli strain Rosetta 2 (DE3) (Novagen). The cells were grown at 37 °C in Luria-Bertani medium (LB ...
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bioRxiv - Plant Biology 2020Quote: ... 2 mM EDTA and 1 % [v/v] protein phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich)) were added at a concentration of 2 mL/g tissue powder ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein sample (400 μg) was reduced by adding 2 μl of Tris(2-carboxyethyl)phosphine (Sigma) and incubating samples at 60 °C for 1 h ...
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bioRxiv - Immunology 2019Quote: WT and Irak1−/− mice were injected intraperitoneally with 10mg per kg of body weight lipopolysaccharide from Salmonella enterica serotype Minnesota (Sigma) dissolved at 1mg/mL in sterile PBS ...
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bioRxiv - Immunology 2021Quote: ... and 1 ml of fresh media (IMDM+10%FBS+1%A/A) was added with either 100 ng/ml LPS (E.coli; Serotype 0111:B4; Sigma Aldrich) or control for 24h ...
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bioRxiv - Neuroscience 2022Quote: WT and TG-G2019S mice were exposed to intraperitoneal (ip) injections of a low dose of lipopolysaccharide LPS (0,1 mg kg−1 Escherichia coli serotype O111:B4) (Sigma-Aldrich), administrated twice a week for 12 weeks (Supplementary Figure 1) ...
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bioRxiv - Molecular Biology 2024Quote: ... pneumoniae serotype 3 (ST3) with or without addition of either Hyaluronidase from bovine testes Type I-S (H3506 Sigma-Aldrich, St ...
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bioRxiv - Molecular Biology 2022Quote: ... For each 10 cm dish, 1 ml virus inoculum was prepared in virus growth media (VGM; containing DMEM, 0.2% bovine serum albumin (Sigma; Cat no. #A8412), 25 mM N-(2-hydroxyethyl ...
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bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
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bioRxiv - Microbiology 2020Quote: ... Aggregated virus was removed by a 0.22µm filter (Millipore). Sub-confluent monolayer of MEFs cultured in DMEM ...
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bioRxiv - Physiology 2020Quote: ... Virus was filtered with a 0.45mm OVDF filter (Millipore). Supernatants were collected and purified using PEG-it (System Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... supernatant containing virus was filtered with 0.22μm filters (Millipore) and store at −80◻ in aliquots.
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bioRxiv - Cancer Biology 2023Quote: ... adherent cells were infected with COL7A1 virus (Sigma Aldrich) (1–5 MOI (multiplicity of infection) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the virus was concentrated using polyethylglycol (Sigma, P4338). Cells were infected in medium containing 5 μg ml−1 polybrene (Millipore ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells were cultured in DMEM-F12 (cat n. D6421, Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: HEK293 cells plated on covered with Poly-D-Lysine (P0899, Sigma-Aldrich) coverslips were co-transfected with pEZY BIFC N-Venus and C-Venus plasmids 48 hours after plating ...