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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 and HeLa cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-GlutaMAX™ -I (Sigma-Aldrich) supplemented with 10% fetal bovine serum (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... and HEK293 control cells were separated by SDS-PAGE using TruPAGE precast gels (4-12%) (Sigma-Aldrich) or 8% Laemmli SDS-gels ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency) ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells from ATCC (ATCC# CRL-1573) cells were cultured in DMEM cell culture media (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... EVI1 or MECOM virus in 12 well plates with 8µg/ml of polybrene (Millipore), spun at 2,000 r.p.m ...
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bioRxiv - Microbiology 2021Quote: ... the virus was concentrated using a 30-kDa Amicon Ultra-15 Centrifugal Filter (Millipore). The viral stocks were stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... the virus supernatant was concentrated using 8% polyethylene glycol (PEG)-6,000 (Sigma-Aldrich, U.S.A). The pellet was resuspended in TNE buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... 1 μL of virus lysate was treated with 250 U of benzonase nuclease (Sigma) at 37°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks were filtered via centrifugation with Amicon Ultra-15 Centrifugal filter unit (Sigma). Infectious viral titers were quantified by plaque assay in Vero-E6 cells in MEM supplemented with 2% FBS ...
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bioRxiv - Microbiology 2022Quote: ... Plates with Towne virus were stained with mouse anti-human CMV IE1 (MAB810, Millipore) then goat anti-mouse IgG-AF488 (Millipore) ...
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bioRxiv - Cancer Biology 2022Quote: ... thawed virus was mixed with culture media +8 μg/mL Polybrene (Millipore Sigma #107689) to infect cells overnight ...
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bioRxiv - Microbiology 2021Quote: ... The virus/antibody mixture was replaced with an overly media containing 1% CMC (Sigma) before incubation for 24 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was dissolved in 0.5 ml virus lysis solution (50 mM TrisHCl; Sigma), 2 mM MgCl2 (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Virus were then buffer exchanged with Amicon Ultra-4 Centrifugal Unit (Millipore Sigma, #UFC800396) to get rid of furin inhibitor in the media.
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bioRxiv - Immunology 2020Quote: ... virus stocks were diluted at indicated MOIs in serum-free DMEM (D5756-500ML, Sigma). 300 μl serum-free media containing virus (or DMEM alone for the control wells ...
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bioRxiv - Cell Biology 2022Quote: ... medium containing the virus was collected and filtered with a 0.45 μm filter (Millipore). The filtered virus solution was directly used for the infection of cell lines or stored in aliquots at −80°C without prior concentration of the virus ...
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bioRxiv - Microbiology 2022Quote: ... and 1:4 800 000 in virus growth medium (VGM) containing MEM (Sigma, 2279), 2 % FBS ...
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Microbiology 2023Quote: ... and resuspending in virus + polybrene (final conc 8 ug/mL, Sigma-Aldrich, TR-1003) or RPMI media + polybrene for the uninfected control at a concentration of 1E6 cells/mL ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed and virus was concentrated using an Optiprep density gradient (Sigma #D1556). Small-scale AAV virus was produced in HEK293T cells grown in 12-well plates by triple-transfection with 0.4μg HiUGE vector ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus (400 µL) was added together with 5 ug/mL of polybrene (Sigma-Aldrich) and cells were centrifuged at 2,000 rpm for 2 h at 30°C ...
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bioRxiv - Cell Biology 2024Quote: ... The virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were transduced with a mix of supernatant containing virus and polybrene (Sigma H9268 suspended at 4mg/ml in sterile water ...
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bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
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bioRxiv - Systems Biology 2021Quote: ... the proteins the samples were reduced with 5 mM Tris(2-carboxyethyl)phosphine (TCEP; Sigma-Aldrich) for 20 minutes in 37 °C ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mg of total protein was then incubated with anti-Flag antibody (M2 monoclonal clone, Sigma) for 1 h with gentle rotation at 4°C and then protein-G coated magnetic beads (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins in the lysate were reduced with 20mM Tris(2-carboxyethyl)phosphine (TCEP) (Sigma-Aldrich) and alkylated with 55mM 2-Chloroacetamide (CAA ...
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bioRxiv - Molecular Biology 2019Quote: The CbAgo WT and DM proteins were expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37°C in LB medium containing 50 μg ml-1 kanamycin and 34 μg ml-1 chloramphenicol until an OD600nm of 0.7 was reached ...
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bioRxiv - Biochemistry 2019Quote: All proteins were expressed in E.coli Rosetta-2 DE3 cells (EMD Millipore, Merck KGaA, Darmstadt, Germany). Cells were transformed via heat shock (42°C for 35 secs ...
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bioRxiv - Neuroscience 2020Quote: ... Neuronal soma and dendrites were identified with microtubule-associated protein 2 (MAP2, Sigma, cat no.#M4403).Neuronal axon projections and their connectivity was identified with neuron-specific class III β -tubulin (Tuj1 ...
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bioRxiv - Cell Biology 2021Quote: ... The immune complexes were collected by incubation (2 h, 4°C) with protein G-agarose (Sigma). Co-IP assays were performed by using Pierce™ Protein G Agarose (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts were reduced with 5 mM 5-Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Sigma-Aldrich) for 2 min at room temperature ...
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bioRxiv - Genetics 2022Quote: ... 2 mgs of total protein were incubated with 20 μL of V5 agarose (Sigma-Aldrich, #7345) as indicated rotating at 4 °C for 2 hrs ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mg of total proteins was incubated with 30 μL M2 FLAG magnetic beads (Sigma-Aldrich) for ATM and 2 mg DNA-PKcs antibody and 30 μL Protein A/G beads for DNA-PKcs ...
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bioRxiv - Plant Biology 2024Quote: ... the total proteins were drawn by extraction buffer with addition 2% protease inhibitors cocktail (Sigma, P9599) and 2% phosphatase inhibitors cocktail (Yeasen,20109-A) ...
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bioRxiv - Physiology 2024Quote: ... The protein samples were denatured using 1x Laemmli sample buffer with 2.5% 2-mercaptoethanol (M3148, Sigma) for each sample at 95 °C for 5 min (STIM1 ...
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bioRxiv - Biophysics 2024Quote: ... and when necessary concentrated the protein to 1∼2 mg/ml (Amicon Ultra 100 KDa, Millipore). For experiments under K+-free conditions ...
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bioRxiv - Neuroscience 2021Quote: The transfected HEK293 cells were seeded on glass coverslips (Matsunami) coated with poly-L-lysine solution (Sigma-Aldrich) then incubated for 4–10 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Neuro-2a (N2a) and HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) high glucose (Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2019Quote: ... HEK293 cell viability after iOn plasmids transfection was assessed by dye exclusion with Trypan blue solution (0.4%, Sigma). FP expression was either assayed by flow cytometry ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) cells were maintained in a 37°C/5% CO2 atmosphere in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293T and HEK293-FT cells were maintained in DMEM supplemented with 10% FBS,1 mM sodium pyruvate (Sigma), 2 mM L-glutamine (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: HEK293-FLP cells stably expressing UBC:DOR-APEX2 were plated onto poly-L-lysine (Sigma Aldrich, Cat # P8920-100ML) coated coverslips in wells of a 24-well plates ...
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bioRxiv - Microbiology 2023Quote: HeLa cells and HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% foetal bovine serum (Sigma) at 37°C in 5% (V/V ...