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Citations for Millipore Sigma :
4051 - 4100 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR was performed in triplicates with the KAPA SYBR® Fast qPCR Master mix (Sigma-Aldrich, KK4600) using a Rotor-Gene Q (Qiagen ...
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bioRxiv - Microbiology 2023Quote: Viral barcodes were amplified in 50 µl PCR reactions using KOD Hot-Start Master Mix (Sigma-Aldrich, 71842). For the viral supernatant samples ...
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bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... using the pPB_EF1_MCS-EGFP_IRES_Puro as a PCR template and utilising primers with the respective knockin-overhangs (ordered from Sigma-Aldrich). Gel-extracted PCR products (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The PCR fragment of each miRNA was cloned into a pT7Blue T-Vector (Novagen, Inc, Madison, WI, USA). The recombinant plasmids were introduced into competent cells of Escherichia coli JM 109 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Indexing PCR reactions were pooled prior to purification with Sera-Mag SpeedBeads™ carboxylate-modified hydrophilic (Sigma-Aldrich). An initial bead concentration of 0.5X was used to remove long fragments that were likely contaminants of fresh DNA ...
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bioRxiv - Microbiology 2023Quote: ... Figure modified from D’Andreano & Francino27.Presence of PCR products was assessed using 0.5% agarose (Sigma-Aldrich, USA, A9539) gel electrophoresis with SYBR Safe DNA Gel Stain (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... Then the sections were washed 3 times in 1×PBS (10 min each wash) and the nuclei were counter-stained with DAPI (D9542-50, Sigma-Aldrich Corp., St. Louis, MO, USA) for 10 minutes of incubation in a dark chamber ...
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bioRxiv - Biophysics 2019Quote: ... The membrane was rinsed three times in TBS-Tw and incubated for 30 min with primary antibody (M2 anti-FLAG from Sigma, diluted 1:500 in TBS-Tw). The membrane was rinsed three more times in TBS-Tw and incubated for 30 min with secondary antibody (HRP-conjugated sheep anti-mouse IgG ...
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bioRxiv - Neuroscience 2022Quote: ... after which the slices were washed with the blocking solution 3 times and stained with the nuclear marker DAPI (Sigma–Aldrich, 1 mg/ml 1:1000) for 15 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by 1-hour incubation at RT with the diluted secondary antibody after which the slices were washed with the blocking solution 3 times and stained with the nuclear marker DAPI (Sigma–Aldrich, 1 mg/ml 1:1000) for 15 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... host cells were washed three times with PBS and lysed in radio-immunoprecipitation assay (RIPA) buffer (Sigma-Aldrich Co., Saint Louis, MO, USA; 50 μL/well). Proteins extracts (15 μg/lane ...
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bioRxiv - Biochemistry 2020Quote: ... Sections were washed three times for 5 min at room temperature and then mounted using Fluoroshield™ with DAPI histology mounting medium (Sigma-Aldrich, Saint-Quentin Fallavier, France). Sections were observed with Axioplan II Fluorescence Microscope (Zeiss ...
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bioRxiv - Molecular Biology 2023Quote: ... the media was quickly aspirated and cells were washed three times with quenching buffer (DPBS supplemented with 10 mM sodium ascorbate, 5 mM Trolox [Sigma-Aldrich], and 10 mM sodium azide). Cells were then scraped in quenching buffer ...
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bioRxiv - Plant Biology 2021Quote: Total antioxidant capacity was measured using a commercially available kit (Total Antioxidant Capacity Assay kit, Sigma, St Louis). 100 mg of leaf tissue from a pool of leaves was frozen at −80°C and homogenized using liquid nitrogen and a mortar and pestle ...
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bioRxiv - Plant Biology 2021Quote: ... total antioxidant capacity (TAC) was analyzed using a commercially available kit (Total Antioxidant Capacity Assay kit, Sigma-MAK187). Ground leaf tissue (0.1 g ...
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bioRxiv - Neuroscience 2024Quote: ... and some of the myelin was labelled with the green-fluorescent cell linker kit (PKH67 labeling kit, Sigma), according to manufacturer’s specifications.
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bioRxiv - Cell Biology 2023Quote: PLA assays were carried out using a Duolink in situ red starter kit mouse/rabbit kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: Leptin - Leptin concentrations were determined by radioimmunoassay using a kit from EMD Millipore (Burlington, MA, kit HL-81K).
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bioRxiv - Molecular Biology 2024Quote: ... we used commercially available ELISA kits: hs-CRP Human C-Reactive Protein ELISA Kit (#RAB0096, Sigma-Aldrich, Germany) and Mercodia Insulin ELISA (#10-1113-01 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lactate Assay Kit II (Sigma-Aldrich, USA), and ATP Detection Kit (Solarbio ...
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bioRxiv - Cell Biology 2020Quote: Cellular Senescence Assay kit (Merck Millipore: KAA002) was used to detect senescent AML12 by SA β-Gal staining as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... A Bicinchoninic Acid Assay (BCA) Kit (Sigma) was used for protein quantification according to the supplier’s protocol and the absorbance was measured at 595 nm ...
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bioRxiv - Immunology 2021Quote: ... Gastrin EIA kit was purchased from Sigma-Millipore ...
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bioRxiv - Immunology 2021Quote: ... by using Gastrin EIA kit from Millipore-Sigma.
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bioRxiv - Genomics 2020Quote: ... The Calcium Colorimetric Assay Kit (Sigma-Aldrich) was used for quantification of calcium ...
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bioRxiv - Developmental Biology 2020Quote: ... and processed according to Kit 91A (Sigma) protocol for monocyte detection ...
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bioRxiv - Microbiology 2019Quote: ... iTRAQ® Reagents Multiplex Kit (Sigma Aldrich), Optima™ LC-MS Grade Trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2019Quote: ... The λDE3 lysogenization kit was from Novagen. Yeast Extract and tryptone were from Becton Dickinson and Company (MD ...
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bioRxiv - Cell Biology 2020Quote: ... Triglyceride Assay kit (Sigma, TG-5-RB), and Glucose (GO ...
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bioRxiv - Cell Biology 2020Quote: ... and Glucose (GO) Assay kit (Sigma, GAGO20). Colorimetric readings were obtained using a microplate spectrophotometer (Molecular Devices ...
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bioRxiv - Microbiology 2021Quote: ... using the ChIP assay kit from Millipore or equivalent homemade solutions ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels (Sigma-Aldrich, TrueGel3D Hydrogel Kit, TRUE7) of ∼10kPa were prepared by thawing of the SLO-DEXTRAN solution ...
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bioRxiv - Cancer Biology 2021Quote: ... The Cell Proliferation Kit II XTT (Sigma) was used according to the manufacturer ...
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bioRxiv - Cancer Biology 2020Quote: ... with a customized Milliplex kit (Merck Millipore).
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bioRxiv - Biochemistry 2020Quote: ... Proximity Ligation Assay kits from Sigma-Aldrich. Lambda protein phosphatase and alkaline phosphatase were from Calbiochem (#539514-20KV ...
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bioRxiv - Microbiology 2021Quote: ... following manufacturer’s instructions (Alkaline phosphatase Kit, Sigma). The intensity of pink stain reflects the amount of alkaline phosphatase in positive cells.
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bioRxiv - Microbiology 2021Quote: ... BCA protein assay kit (Sigma-Aldrich, USA), RNeasy kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: AST Activity Assay Kit (Sigma-Aldrich MAK055) was used to determine aspartate aminotransferase activity per manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... were detected by ELISA Kits (from Millipore) within 24h according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-vimentin (kit # 17-10195, Millipore), goat polyclonal anti-rabbit-HRP (Biorad ...
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bioRxiv - Cancer Biology 2021Quote: ... The Duolink PLA kit (DUO92007, Sigma-Aldrich) was used according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Leukocyte (TRAP) Kit (Sigma-Aldrich, Burlington, US) at 37°C ...
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bioRxiv - Immunology 2022Quote: ... or Enzymatic Protein Biotinylation Kit (Sigma-Aldrich). SARS-CoV-2 RDB protein was also coupled to DyLight 650 using the DyLight® Amine-Reactive Dyes kit (Thermo Fisher scientific).
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bioRxiv - Neuroscience 2022Quote: ... The ApopTag TUNEL stain kit (Millipore #S7165) was used for Figures 3B ...
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bioRxiv - Physiology 2019Quote: Spectrum Plant Total RNA Kit (Sigma Aldrich) was added into the host root sample for RNA extraction ...
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bioRxiv - Genetics 2019Quote: ... the latter using Apoptag-peroxidase kit (Millipore).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... following the manufacturer’s protocol (ECL Kit, Millipore) and digital images were acquired using a ChemiDoc XRS+System (BioRad) ...
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bioRxiv - Physiology 2020Quote: ... Serum triglyceride determination (TG) kit (Sigma, USA) and free fatty acid assay (FFA ...
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bioRxiv - Molecular Biology 2021Quote: ... KAPAR SYBR Fast Universal kit (Sigma KK4602)