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Citations for Millipore Sigma :
3751 - 3800 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Proteins were separated by 8% or 10% Tris-Glycine-SDS page gels for 1 h and transferred on PVDF membrane (0.45 um, Millipore). Then blocked the membrane with 5% skimmed milk in TBS-T buffer (20 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2022Quote: ... 8-16-weeks-old mice were treated with tamoxifen-containing water (1 mg/ml) (T5648, Sigma-Aldrich, Darmstadt/Germany) to induce Cre recombinase activity ...
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bioRxiv - Genomics 2020Quote: ... cells were lysed on ice in 5 ml lysis buffer (10 mM Tris-HCl, pH 8, 10 mM NaCl, 0.2% Igepal NP-40 (Sigma), 1× cOmplete protease inhibitor (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... 8 µg of antibody (anti-acetyl-histone H4, Millipore Sigma 06-598; anti-acetyl-histone H2A.Z, Millipore Sigma Abe1363) was then added and tubes were rotated overnight at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Fixed cells were permeabilized in 1ml lysis buffer (10mM Tris–HCl pH 8, 10mM NaCl, 0.2% NP-40, protease inhibitor cocktail (Sigma) for 15 min on ice ...
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bioRxiv - Cell Biology 2019Quote: ... GST (clone 8-326, MA4-004 from Thermo Fischer, 1:1000) and Cyclin B (C8831 from Sigma, 1:1000). The secondary antibodies used for Western blotting were goat ⍰ -mouse IgG HRP conjugate (170-6516 from Bio-Rad ...
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bioRxiv - Physiology 2019Quote: On average 8 mg of muscle tissue was pulverized in liquid nitrogen and mixed with the lysis buffer containing protease inhibitor cocktail (8 M Urea, 2M Thiourea, 4% CHAPS, 1% Triton X-100, 50 mM Tris, pH 8.5 (Sigma)) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were subsequently fixed in 8% formaldehyde and stained using the Alkaline-Phosphatase kit (86R 1KT, Sigma-Aldrich, Germany) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... ~2×108 cells were collected and lysed in a hypotonic buffer (5 mM PIPES pH 8, 85 mM KCl, 0.5% NP-40) supplemented with Complete Protease Inhibitor Cocktail (Sigma) for 20 minutes ...
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bioRxiv - Neuroscience 2019Quote: Fixed brains were washed in phosphate buffered solution and embedded in 8% (w/v) agarose (low melting point, Sigma) in water ...
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bioRxiv - Microbiology 2019Quote: ... Phage pellet was resuspended in 2 ml of SM buffer (100 mM NaCl, 8 mM MgSO4, 50 mM Tris pH 7.5 (Sigma) and treated with 0.25 μg of RNAse A (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... and resuspended in 100 μl of SM buffer (100 mM NaCl, 8 mM MgSO4, 50 mM Tris pH 7.5 (Sigma)) ...
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bioRxiv - Cancer Biology 2020Quote: ... LPS stimulated B-cells were transduced by retroviral pool spin infection in 8 μg/μl of polybrene (Sigma-Aldrich). Each retroviral pool contained vectors expressing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 70 μl of 8 M urea (Fisher Cat #: U15500) in 50 mM ammonium bicarbonate (Sigma Cat#: 09830-500G), and stored at −80°C before proteomics analysis ...
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bioRxiv - Genomics 2019Quote: ... cells were collected at day 8 and RNA was extracted using GenElute Mammalian Total RNA MiniPrep Kit (Sigma #RTN350). cDNA was synthesized using oligodT primers using SuperScript II Reverse Transcriptase (Thermo Fisher Scientific #18064014) ...
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bioRxiv - Neuroscience 2019Quote: ... 8-week-old male and female mice were anesthetized with 4% isoflurane and injections of 1.2% avertin (Sigma-Aldrich). Mice were perfused with 10 mL of ice-cold cold N-methyl-D-glucamine (NMDG)-based cutting solution ...
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bioRxiv - Cell Biology 2020Quote: ... 3-Isobutyl-1-methylxanthine (15879) and 8-Bromoadenosine 3’,5’-cyclic monophosphate sodium salt (B7880) were purchased from Sigma. Phosphodiesterase inhibitor Tocriset containing Milrinone ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells were transfected with SEC61-GFP expression vector in 8 well chamber slide (Millipore Sigma, Millicell EZ SLIDE) and ...
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bioRxiv - Neuroscience 2021Quote: ... to assess protein concentration and then solubilized in 4x loading dye (250 mM Tris-HCl pH 6.8, 40% glycerol, 0.008% bromophenol blue, 8% SDS; all from Sigma-Aldrich). All samples were boiled at 65°C for 10 minutes and then equal amounts of each sample were separated using SDS-PAGE and subsequently blotted on nitrocellulose membranes using the Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... then triturated and plated on poly-L-lysine coated 8-well glass chambers (Sigma, P2636, Cellvis, C8-1-N) at a density of 25,000 cells/well in 5 % horse serum (Gibco 26050-088 ...
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bioRxiv - Cell Biology 2021Quote: ... retroviral particles were produced as described (Guimbal et al., 2019) and RAW264.7 cells were infected for 24h in 8 μg/ml polybrene (Sigma). Alternatively ...
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bioRxiv - Biochemistry 2021Quote: ... These were used to transduce A549 cells in the presence of 8 μg/mL of hexadimethrine bromide (Polybrene, Sigma). Following 24 hours of transduction ...
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bioRxiv - Microbiology 2021Quote: ... Samples were kept in dark and cold conditions while prefiltered by gravity through 10 μm nylon mesh (KC Denmark) and then onto 8-12 47 mm 0.45 μm Durapore filters (Millipore) using a vacuum pump ...
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bioRxiv - Molecular Biology 2020Quote: ... Hypertrophic remodelling was induced in 8-10 week old male mice animals by administration of isoproterenol (Iso, Sigma-Aldrich) at 10 mg/kg/day for one week via osmotic mini-pumps (Alzet ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL of bead solution was removed and replaced with 200 μL of phenol:chloroform:isoamyl alcohol (Sigma; pH 7-8) before the stool was added ...
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bioRxiv - Cell Biology 2020Quote: ... The washed beads were resuspended in the same buffer containing HRV 3C protease (8 units/ml in final; Novagen) and incubated without agitation (4°C ...
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bioRxiv - Microbiology 2021Quote: ... 1.2×108 cells were transduced with lentivirus-packaged sgRNA library at MOI 0.3 in the presence of 8 μg/ml polybrene (Sigma) to achieve ~500-fold overrepresentation of each sgRNA ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with selection medium (SM, 8 mM L-glutamine and phenol red, with 0.5 mg/mL G-418, Sigma). Cell pools were maintained at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... MTX (Applied Biosciences) Transduction of JJN3 MMCL was performed in the presence of 8 μg/ml polybrene (Sigma, UK). On day 3 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were dissolved in 100% methanol doped with NEM [8 mM,1 mg/mL] (Sigma-Aldrich, cat. no. E1271) (110) ...
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bioRxiv - Biophysics 2020Quote: ... Ovaries were disrupted using RNase-free 1.5mL pestles (SLS) in lysis buffer (50 mM Tris-HCl pH 8 (Sigma), 150 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 8-week-old rats received a single intravenous dose of 60 mg/kg streptozotocin (STZ, Sigma Chemical, MO, USA) diluted in STZ-vehicle (cold citrate buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were transduced in DMEM medium supplemented with 10 mM HEPES and 8 μg/ml of Polybrene (Sigma H9268) immediately prior to the conditioned medium being added.
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bioRxiv - Biophysics 2022Quote: ... 10% glycerol) to a conductivity of 85 uS/CM and loaded onto an 8-mL SP-Sepharose column (Sigma). Cdc6 was eluted in 20-mL steps of buffer A containing either 0.2 M ...
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bioRxiv - Neuroscience 2022Quote: ... First 400 µL of 100 mM pentafluorobenzyl bromide (PFB-Br, CID: 1765-40-8, Sigma-Aldrich, Catalog number: 101052), prepared in acetonitrile (Fisher Chemical ...
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bioRxiv - Genetics 2022Quote: Fipronil (CAS# 120068-37-3) and Spinosad (CAS# 168316-95-8) were both obtained as analytical standards from Sigma. Insecticide bioassays were performed as described previously [20] ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated by SDS-PAGE with 8% polyacrylamide gel and transferred to PVDF membrane (Millipore, Billerica, MA, USA). Membranes were blocked with 5% skim milk in Tris-buffered saline (20 mM Tris [pH8.0] ...
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bioRxiv - Bioengineering 2022Quote: ... PeproTech], 10 ng/mL keratinocyte growth factor [KGF; PeproTech], 50 nM dexamethasone [Sigma], 0.1 mM 8-Bromo cAMP [Sigma] ...
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bioRxiv - Biochemistry 2021Quote: ... Ovaries and testes were disrupted using RNase-free 1.5 mL pestles (SLS) in lysis buffer A (50 mM Tris-HCl pH 8 (Sigma), 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM PMSF and 0.01% Triton X-100) with 8 μl of SIGMA FAST protease inhibitor cocktail (Sigma-Aldrich). The pellets were then sonicated at 30% amplitude for 10 sec with 10 sec intervals on ice and were then centrifuged for 30 min at 4°C and 14,000rpm ...
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bioRxiv - Microbiology 2021Quote: ... Buffer exchange into TBS pH7.4 and 1 mg/ml Amphipol 8-35 using Amicon Ultra-4 centrifugation filters with a 100kDa cutoff (Millipore). The concentration of Amphipol 8-35 was brought up to 35mg/ml and incubated at room temperature for 4 hours then Bio-Beads SM-2 (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... and transported back to the laboratory for immediate filtering (i.e. within maximum 8 hours after retrieval) through a 0.2 µm polyethersulfone filter (Steritop; EMD Millipore). Filters were frozen at −20 °C until DNA extraction ...
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bioRxiv - Plant Biology 2021Quote: ... The 50 µl pre-washed with washing buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 0,1% Tween-20) M2 anti-FLAG magnetic beads (Sigma) was added to supernatant ...
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bioRxiv - Molecular Biology 2020Quote: ... KCl 5 mM, glucose 8 mM, HEPES 10 mM, MgCl2 1.2 mM, and CaCl2 1.5 mM; all supplied by Sigma Aldrich) containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM ...
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bioRxiv - Neuroscience 2021Quote: ... at a plating density of 350 cells/well in 8-well chamber slides pre-coated with 100μg/ml poly-D-lysine (#A-003-M, Sigma). CNS myelin extracts (CME ...
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bioRxiv - Biophysics 2021Quote: ... and an oxygen scavenging system45,46 (final concentrations in buffer: 8 mg/mL glucose, 0.15 mg/mL catalase (from Aspergillus niger: Millipore Sigma, formerly EMD Millipore ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse embryos were cultured until the 8-cell stage in KSOM medium supplemented with 0.4% bovine serum albumin (Millipore), after which the medium was changed to Cook Blasto ® (Cook Ireland Ltd ...
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bioRxiv - Cell Biology 2022Quote: ... and subsequently scraped with ice cold RIPA buffer (50 mM Tris pH 8, 150 mM NaCl, 0.5% deoxycholate, 0.1% SDS, 1% NP-40; all chemicals from Sigma Aldrich) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Immunology 2022Quote: ... Cell Counting Kit-8 (CA1210) and D-Luciferin (L6882) were all purchased from Sigma-Aldrich (Saint Louis, MO, USA). Lipofectamine2000 was purchased from Invitrogen (11668019 ...