Labshake search
Citations for Millipore Sigma :
3751 - 3800 of 7599 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... iFluor 647-conjugated sAvPhire monovalent streptavidin (catalytic soluble protein uptake, Millipore Sigma SAE178-100UG); iFluor 488-conjugated sAvPhire monovalent streptavidin (catalytic soluble protein uptake ...
-
bioRxiv - Bioengineering 2024Quote: ... iFluor 488-conjugated sAvPhire monovalent streptavidin (catalytic soluble protein uptake, Millipore Sigma SAE176-100UG); IRDye 680RD Donkey anti-goat (Western blotting ...
-
bioRxiv - Cancer Biology 2023Quote: ... Proteins were semi-dry blotted onto a 0.2 µM nitrocellulose membrane (Sigma cat GE10600001) and protein levels were detected by specific antibodies directed against ...
-
bioRxiv - Microbiology 2023Quote: ... The supernatant containing cleaved proteins was concentrated using a 30K MWCO concentrator (EMD Millipore) to 1 ml ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were separated by SDS-PAGE and were transferred to PVDF membranes (Millipore). All membranes were incubated with the indicated primary antibodies diluted in TBS-T (20mM Tris pH 7.5 ...
-
bioRxiv - Microbiology 2024Quote: ... 80 μl agarose protein G with salmon sperm DNA slurry (Millipore Cat. 16-201) was added for pre-clearance ...
-
bioRxiv - Microbiology 2024Quote: ... 80 μl agarose protein G with salmon sperm DNA slurry (Millipore Cat. 16-201) was added for pre-clearance ...
-
bioRxiv - Cell Biology 2024Quote: mESC protein samples were isolated from mESC pellets using ice-cold RIPA buffer (Sigma) in the presence of freshly added 1% protease and phosphatase inhibitor cocktail (Invitrogen) ...
-
bioRxiv - Molecular Biology 2024Quote: C2C12 protein was extracted from cells using RIPA buffer (Sigma-Aldrich, UK. Cat # R0278) using the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich, Germany).
-
bioRxiv - Molecular Biology 2024Quote: ... biotinylated proteins bound to streptavidin beads were resuspended in 100 mM ammonium bicarbonate (Sigma) and digested with 2 µg Trypsin (Promega ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (EMD Millipore, catalog no. WBKLS0500) and ChemiDoc Imaging System (Bio-Rad Laboratories ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were reduced using 5 mM tris (2-carboxyethyl) phophine hydrochloride (TCEP) (Sigma; 75259) for 10 min at 95° C and alkylated using 10 mM 2-iodoacetamide (Sigma ...
-
Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... The eluted proteins were further concentrated using Amicon® Ultra-4 centrifugal filter (Millipore) and contaminants were removed using Gel-filtration chromatography ...
-
bioRxiv - Neuroscience 2024Quote: ... 100 µg of proteins were reduced with 10 mM dithiothreitol (Sigma-Aldrich, MO, USA) at 37 °C for 60 min ...
-
bioRxiv - Neuroscience 2024Quote: Proteins was isolated from cultured cells or brain tissues with RIPA buffer (Sigma, R0278) including protease inhibitors cocktails ...
-
bioRxiv - Neuroscience 2024Quote: ... the proteins were eluted with wash buffer containing 1.5% β-mercaptoethanol (Sigma-Aldrich, M3148) at 37 °C for 0.5 h with agitation (350 rpm ...
-
bioRxiv - Cell Biology 2024Quote: ... the proteins on the membranes were detected by enhanced chemiluminescence reagents (WBKLS0500, Millipore, USA).
-
bioRxiv - Genetics 2024Quote: ... Then the resolved proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore, IPVH00010). The membranes were blocked ...
-
bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto 0.45 nm pore polyvinylidene fluoride (PVDF) membrane (Millipore, USA) using an eBlot™ L1 wet transfer (GenScript Biotech ...
-
bioRxiv - Immunology 2024Quote: ... Proteins were transferred to a 0.2 μm polyvinylidene fluoride membrane (PVDF, Millipore, Billerica, USA) using a Bio-Rad semi-dry instrument ...
-
bioRxiv - Immunology 2024Quote: ... protein bands on the blots were developed with chromogenic substrate SigmaFast BCIP/NBT (Sigma). All blots were run at least twice to check for reproducibility ...
-
bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
-
bioRxiv - Microbiology 2024Quote: Recombinant ϕLf-UK PSB15 protein was expressed in B834 (DE3) pLysS cells (EMD Millipore) by transforming pR-PSB15 ...
-
bioRxiv - Microbiology 2024Quote: ... Bound protein was eluted with 100 µL of 1.5 mg/mL FLAG peptide (Millipore) resuspended in wash buffer ...
-
bioRxiv - Genetics 2023Quote: ... 20 μL Magna ChIP™ Protein A+G Magnetic Beads (Sigma-Aldrich 16-663) was washed four times with 1X DPBS supplemented with 5 mg/mL BSA and subsequently crosslinked to 5 μg antibody (Anti-FOXA1 antibody ...
-
bioRxiv - Genetics 2023Quote: ... and the supernatants were precleared with 10 μL of Protein A-coated agarose (Millipore). The precleared supernatants were then divided into two equal parts and incubated with 20 μL 14-3-3 polyclonal antibody and IgG overnight at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
-
bioRxiv - Cell Biology 2023Quote: ... protein lysates were extracted from BMDMs using Radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich, R0278) complimented with protease inhibitor (Roche Diagnostics ...
-
bioRxiv - Genomics 2023Quote: ... the supernatants were collected and protein concentration was measured by Bradford assay (Sigma-Aldrich). Extracts were boiled in presence of SDS loading buffer and analyzed by Western blotting.
-
bioRxiv - Evolutionary Biology 2024Quote: ... The crude protein lysate concentration was quantified by bovine serum albumin (BSA) (Sigma-Aldrich) and 25 μg crude extract was loaded on NuPAGE™ 3-8% Tris-Acetate gels (ThermoFisher ...
-
bioRxiv - Molecular Biology 2023Quote: ... The proteins were concentrated using 10 kDa cut off Amicon centrifugal filters (Millipore, USA).
-
bioRxiv - Bioengineering 2024Quote: ... Proteins were concentrated by centrifugation through Amicon Ultra 15 filter units (Millipore, Burlington, MA) with a 30-kD cutoff and then dialyzed against 3×PBS ...
-
bioRxiv - Systems Biology 2023Quote: ... Proteins were digested overnight at 37C with trypsin (1:50 µg/µg) (Sigma-Aldrich) and lysyl endopeptidase (1:75 µg/µg ...
-
bioRxiv - Microbiology 2023Quote: ... Protein molecular weights were estimated from elution volumes using BSA (66 kDa) (Sigma-Aldrich) and ovalbumin (45 kDa ...
-
bioRxiv - Neuroscience 2023Quote: ... and glial fibrillary acidic protein (mouse anti-GFAP, 1:400, Sigma-Aldrich, cat. #: G3893), and cell nuclei were counterstained using Hoechst (10 µg/mL) ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were eluted in lysis buffer supplemented with 5% fresh β-mercaptoethanol (Sigma, M3148) and 1X SDS-PAGE loading buffer (5X SDS-PAGE loading buffer ...
-
bioRxiv - Immunology 2022Quote: ... Proteins were reduced with the addition of 3 μL of 250 mM dithiothreitol (Sigma) at 37°C for 45 min and alkylated with 4.5 μL of 500 mM chloroacetamide for 30 min at room temperature in the dark ...
-
bioRxiv - Immunology 2023Quote: ... The boiled proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Millipore). The membranes were blocked in TBST (Tris-buffered saline ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM TCEP and mixed with 1:5000 SYPRO Orange Protein Gel Stain (Sigma). 50 μL of assay mixture was dispensed per well in a white bottom qPCR plate and each condition was performed in technical triplicate ...
-
bioRxiv - Immunology 2023Quote: ... The supernatant was filtered through a 0.45μm ultra-low protein binding filter (Merck Millipore). Aliquots were stored at −80 °C.
-
bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 minutes at 60°C with 10 mM dithiothreitol (Sigma) in 25 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
-
bioRxiv - Microbiology 2023Quote: ... the separated proteins were transferred onto a methanol activated 0.45 μM PVDF membrane (Millipore) at 70V for 120 minutes at 4 °C in Transfer Buffer (192 mM Glycine ...
-
bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were detected with a streptavidin-HRP conjugate (Millipore-Sigma, Cat#: OR03L-200UG), used at 1:2,000 dilution (see Reagent Table).
-
bioRxiv - Molecular Biology 2023Quote: ... The remaining protein was run through a 50 kDA cut off spin concentrator (Millipore) and dialyzed into 2X storage buffer (48 mM HEPES ...
-
bioRxiv - Cancer Biology 2023Quote: ... The M-protein solutions were diluted 1:1 with 8 M Urea (Sigma Aldrich) in Tris.HCl (pH=7 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted in two column volumes of 0.2 mg/ml 3xFLAG peptide (Sigma) and dialyzed against 50 mM HEPES pH 7.4 ...
-
bioRxiv - Biochemistry 2022Quote: ... proteins were concentrated to >10 mg/mL using Amicon Ultra-15 Centrifugal Filters (Millipore). For some of the mutants assayed in this study ...
-
bioRxiv - Biochemistry 2023Quote: ... The proteins separated by SDS-PAGE were transferred to PVDF membranes (Millipore, Cat#PVH00010). After blocking with 5% (w/v ...
-
bioRxiv - Cancer Biology 2023Quote: ... Proteins were then wet transferred to a polyvinylidene difluoride (PVDF) membrane (IPVH00010, Sigma Aldrich). The membrane was incubated in blocking buffer consisting of 5% milk in PBS plus 0.1% Tween-20 ...