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Citations for Millipore Sigma :
3901 - 3950 of 7599 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 100 mM Tris pH 8.5 with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Bioengineering 2024Quote: ... After dialysis the protein was concentrated through Amicon centrifugal filters (Millipore, Burlington, MA, USA) with a molecular weight cutoff of 3 kDa ...
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bioRxiv - Biophysics 2024Quote: ... Heterologous protein expression was then initiated with 0.02% w/v L-Arabinose (Sigma A3256) overnight at 30 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were resolved by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). Immunoblots were developed using Western Lightning Chemiluminescence Reagent Plus (Advansta ...
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bioRxiv - Physiology 2024Quote: ... The membrane with transferred protein was stained with Ponceau S stain (Sigma-Aldrich #P7170) to check the quality of transfer and protein load ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein bands were visualised by adding Immobilon Forte Western HRP substrate (Millipore #WBLUF0100) on a ChemiDoc XRS+ (BioRad).
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bioRxiv - Biochemistry 2024Quote: ... Protein fractions were collected and concentrated in a 10 kDa MWCO concentrator (Millipore Sigma), flash frozen in liquid nitrogen and stored at −80 °C.
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... a primary antibody against the HCoV-OC43 N protein (Millipore #MAB9013; diluted 1:500) was used ...
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bioRxiv - Immunology 2024Quote: ... Protein expression was induced by adding isopropyl β-D-thiogalactopyranoside (IPTG; 2.0 mM, Sigma). Incubation was continued for another 4h at 37°C in a shaking incubator ...
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bioRxiv - Immunology 2024Quote: ... The first two immunizations included 100µg/100µl OVA protein (Sigma Aldrich, 9006-59-1) in complete Freund’s adjuvant (CFA ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated on 5.5% polyacrylamide (PAA) gel and transferred on PVDF membrane (Millipore) using semi-dry blotting for 60 min ...
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bioRxiv - Neuroscience 2024Quote: ... cell lysates were harvested from hMGLs using RIPA protein lysis buffer (#20-188; Millipore) containing 1X protease and 1X phosphatase inhibitors (#PI78443 ...
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bioRxiv - Neuroscience 2024Quote: ... and immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Chemi Premium (VWR International) ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were separated by SDS-PAGE and transferred to a PVDF membrane (Millipore). The blots were blocked with 5% non-fat milk ...
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bioRxiv - Biochemistry 2024Quote: ... The final concentration of the protein as estimated from the BCA test (Sigma Aldrich) was 15.4 mg/ml.
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bioRxiv - Biochemistry 2024Quote: ... The proteins separated by SDS-PAGE were transferred to PVDF membranes (Millipore, Cat#PVH00010). After blocking with 5% (w/v ...
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bioRxiv - Systems Biology 2024Quote: ... followed by filtration using 96-Well protein precipitation filter plate (Sigma-Aldrich, 55263-U). Retention index (RI ...
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bioRxiv - Pathology 2024Quote: Protein samples were extracted using radioimmunoprecipitation buffer containing inhibitors of proteases (cOmplete, Sigma-Aldrich) and phosphatases (PhosSTOP ...
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bioRxiv - Cell Biology 2024Quote: Protein content in mitochondria-enriched fractions were estimated using Bradford Assay reagent (Sigma-Aldrich) followed by solubilisation as previously reported by Rawat et al21 ...
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bioRxiv - Genetics 2024Quote: ... proteins on the SDS-PAGE were transferred to a PVDF membrane (Millipore, Billerica, MA) at 100V for 3 hours at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Purified His-tagged proteins were concentrated and desalted using Amicon centrifugal filters (Millipore, UFC500396) in TBS buffer and stored in storage buffer (30 mM Tris–HCl ...
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bioRxiv - Plant Biology 2024Quote: ... The eluted protein was further concentrated using a spin concentrator (10 kDa Amicon, Millipore) and rebuffered in 20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentrations were determined by using a SpectraMax QuickDrop and bovine serum albumin (Sigma) as the reference standards for analysis.
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bioRxiv - Neuroscience 2024Quote: ... and then proteins were transferred to an Immobilon PVDF membrane (EMD Millipore, Temecula, CA) at 25V for 90 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were alkylated by addition of 100 μl of 50 mM iodoacetamide (Sigma RPN6320V) prepared fresh in 50 mM NH4HCO3 pH 7.5 buffer and allowed to react in the dark at 20 °C for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... and bounded proteins were eluted with NETN buffer containing 2 mg/ml biotin (Sigma-Aldrich) for 1 h twice at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted kinases (proteins) were concentrated with Amicon Ultra-4 (10K cutoff) spin columns (Millipore), purified by removing the detergent using methanol/chloroform extraction ...
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bioRxiv - Cell Biology 2020Quote: ... PreScission protease-eluted supernatant was added to S-protein agarose beads (EMD Millipore, Burlington, MA) and incubated rocking for 3 hours at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein detection was performed using chemiluminescent substrate (ECL™ Western Blotting Reagents, Sigma-Aldrich, GERPN2106) with film exposure (HyBlot CL® Autoradiography Film ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated with Amicon Ultra-15 Ultracel 10k centrifugal filter units (Merck Millipore, #UFC901024). Purification of cytoplasmic actin ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies against cytoskeletal proteins (vimentin, actin and β-tubulin were from Sigma (St.Louis, MO, USA). The mitochondrial marker MitoTracker® ...
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bioRxiv - Cell Biology 2020Quote: ... 4-dinitrophenylhydrazine using protein carbonyl content assay kit according to the manufacturer’s instructions (Sigma, USA). The optical density of the adduct was examined at 375 nm ...
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Mammalian-unique eIF4E2 maintains GSK3β proline kinase activity to resist senescence against hypoxiabioRxiv - Cell Biology 2020Quote: ... Biotinylated proteins were pulled down by streptavidin-agarose beads (50 μL beads/sample, S1638, Sigma) overnight to purify nascent proteins for western blotting analysis.
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bioRxiv - Immunology 2021Quote: ... Separated proteins were transferred to a nitrocellulose membrane (GE 10600002) or PVDF membrane (Millipore IPVH00010). Membranes were blocked for a minimum of 0.5 hour at room temperature in a 5% (w/v ...
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bioRxiv - Immunology 2021Quote: The 96-well polystyrene microplates were coated with 500 ng of protein A (Sigma-Aldrich) in coating buffer for four h at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: Protein extracts were prepared with RIPA buffer supplemented with protease and phosphatase inhibitors (Sigma-Aldrich) using a minipestle or Bioruptor Pico (Diagenode) ...
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bioRxiv - Molecular Biology 2020Quote: ... was filtered through a frit filter plate (96-Well Protein Precipitation Filter Plate, Sigma Aldrich), and the effluent was collected and evaporated to dryness and the residue was dissolved in 20 ▯L of a 40:60 MeOH:H2O v/v mixture containing the same 13C-PFAS injection standards ...
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bioRxiv - Microbiology 2019Quote: ... the DNA sequences encoding the proteins without the signal sequence were cloned into pET28a (Novagen) using the NcoI and XhoI restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoreactive protein signals were visualized using stable peroxide solution and luminol/enhancer solution (Millipore, USA). Silkworm α-tubulin protein was used as an internal control.
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bioRxiv - Evolutionary Biology 2021Quote: ... triglyceride assays (Pointe Scientific T7532-120, T7531-STD) and soluble protein assays (Sigma B9643-1L) were then carried out in triplicate according to the kit instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein lysis was incubated on ice with 50 U of Benzonase Nuclease (Millipore, #E1014-5KU) for 20 min and at 37 °C for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Heart lysates were first pre-cleared with 20 μl of packed protein A beads (Sigma) per lysate on a rocker or tumbler at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... bound proteins from the pooled washed resin were eluted with 100μg of FLAG peptide (Sigma) in FLAG wash buffer ...
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bioRxiv - Physiology 2022Quote: ... Oocytes expressing desired proteins were harvested in ice-cold CelLytic M lysis buffer (Sigma-Aldrich) supplemented with proteinase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were eluted in HBS containing 1 mM DTT and 2.5 mM desthiobiotin (Sigma-Aldrich). Proteins were spun at 100,000x g to remove aggregates before use in assays ...
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bioRxiv - Neuroscience 2021Quote: ... 100 μg of total protein for each group were reduced with 5 mM dithiothreitol (Sigma) for 30 min at 56 °C and then alkylated with 11 mM iodoacetamide (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... protein-A sepharose and ANTI-FLAG® M2 Affinity Gel beads (Sigma Aldrich, Cat# A2220) were washed three times with 1x PBS and two times with IP100 buffer (25 mM Tris-HCl 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred to a 0.45 µm PVDF membrane (EMD Millipore, Billerica, MA, USA), and the blot was probed with anti-AGO2 mouse monoclonal antibody (Wako Pure Chemical Corporation ...
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bioRxiv - Molecular Biology 2020Quote: ... the separated proteins by SDS-PAGE were transferred to polyvinylidene difluoride (PVDF) membrane (EMD Millipore), washed with TBST for 5 minutes and then were blocked with 5% BSA in TBST[0.1%(v/v ...
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bioRxiv - Molecular Biology 2021Quote: N-protein was dyed by adding (3:1) Atto 488 NHS ester (Millipore Sigma 41698) to purified protein and incubating mix at 4°C for 1 hour with rocking ...