Labshake search
Citations for Millipore Sigma :
3701 - 3750 of 7599 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... The recombinant protein was purified by affinity chromatography using anti-FLAG affinity resin (Sigma). The purified protein was precipitated by 20% trichloroacetic acid ...
-
bioRxiv - Molecular Biology 2020Quote: ... and then with protein A agarose/salmon sperm DNA slurry (Millipore, Cat# 16-157). The beads were washed and eluted as described43 ...
-
bioRxiv - Biophysics 2020Quote: ... The protein was then eluted with lysis buffer containing imidazole (Sigma-Aldrich Co., USA) at different concentrations (20 mM ...
-
bioRxiv - Microbiology 2022Quote: ... The protein samples were concentrated by ultrafiltration using an Amicon Ultra-3KDa concentrator (Millipore). For further purification ...
-
bioRxiv - Neuroscience 2022Quote: ... Soluble proteins were then incubated with 1 μg of anti-FLAG antibody (F1804, Sigma) or 1 μg of mouse IgG (sc-2025 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein lysate was then blotting on a polyvinylidene fluoride (PVDF) membrane (Millipore, MA, USA) and transferred for 1 hour at 15V using Trans-Blot® SD Semi-Dry Transfer Cell (BioRad) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and the proteins were separated in SDS-PAGE and transferred to PVDF membrane (Millipore) and subsequently probed with respective antibodies.
-
Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... Purified proteins were concentrated using Amicon Ultra-10k centrifugal filter device (Merck Millipore Ltd.), and the concentration was determined using Bradford assay kit (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2022Quote: ... The supernatant was collected and pre-cleaned with Protein G Agarose beads (Sigma-Aldrich) for 1-2 h at 4 °C rotating to enhance specificity and the total protein content was quantified by Bradford assay ...
-
bioRxiv - Molecular Biology 2022Quote: ... The eluted proteins were concentrated by Amicon Ultra Centrifugal Filter 50 kDa (Merck Millipore) and purified on a Superose 6 10/300 GL column (GE Healthcare ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2022Quote: We conducted SEC on the SEC protein standards (molecular weights 12.4-200 kDa; Sigma) including β-amylase from sweet potato (200 kDa) ...
-
bioRxiv - Biophysics 2022Quote: ... The protein was concentrated by centrifugal filtration (Amicon, EMD Millipore, 10-kDa MW cutoff) at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein recovery was maximized by adjusting the concentration of imidazole (Sigma cat# l5513-5G) as follows ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved in SDS-polyacrylamide gels and blotted onto PVDF membranes (Millipore/Merck) or nitrocellulose membranes (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... The proteins were transferred onto an Immobilon-fl polyvinylidene difluoride (PVDF) membrane (Sigma-Aldrich) at 100V for 1 hour on ice in NuPAGE Transfer Buffer (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by SDS-PAGE and transferred onto Immobilon-FL Transfer Membrane (Millipore). The membranes were blocked in PBS-T (PBS containing 0.05% Tween 20 ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: Total cell protein was extracted with lysis buffer (RIPA, Sigma-Aldrich, catalog no. R0278) containing 1% protease-phosphatase inhibitor cocktail (Thermo Scientific ...
-
bioRxiv - Immunology 2022Quote: ... Proteins separated by SDS–PAGE were transferred onto Immobilon-FL PVDF membranes (EMD Millipore) at 100 V for 1 h at 4 °C ...
-
bioRxiv - Plant Biology 2022Quote: ... Protein ubiquitination was tested by immunoblots and FLAG-HRP antibody (Sigma, Catalog number A8592).
-
bioRxiv - Microbiology 2022Quote: ... the total protein concentration of each sample was measured via Bradford assay (Sigma Aldrich). Samples were subsequently diluted to a final concentration of 0.5 µg total protein/µl ...
-
bioRxiv - Microbiology 2022Quote: ... The TS fraction was concentrated using Amicon protein concentrators with a 10KDa cutoff (Sigma).
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins were detected with an enhanced chemiluminescence assay kit (ECL Plus, Millipore Corporation, USA). Signals were visualized using ImageLab 1.46 (BioRad ...
-
bioRxiv - Molecular Biology 2022Quote: ... Not protein depletions were obtained by addition of auxin (3-indoleacetic acid, Sigma-Aldrich I2886 ...
-
bioRxiv - Microbiology 2022Quote: ... and cells were lysed with 50 μL of 1 × BugBuster Protein Extraction Reagent (Sigma). Lysates clarified at 21 ...
-
bioRxiv - Neuroscience 2022Quote: ... and 15 μL of Protein-G Sepharose (Millipore/Cytiva Cat#GE-17-0618-05), washed three times in ice-cold wash buffer (0.1% Triton X-100 in 150 mM NaCl ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the Bradford reagent (Sigma Aldrich, St. Louis, MO, USA). Equal amounts of proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes by using Trans-Blot Turbo™ Transfer Pack (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
-
bioRxiv - Microbiology 2023Quote: ... SPA-tagged proteins were detected with monoclonal anti-Flag M2 antibody purchased from Sigma. YbgF protein was used as an internal control and revealed with polyclonal anti-YbgF antibodies ...
-
bioRxiv - Microbiology 2023Quote: ... The DNA protein complex was immunoprecipitated with either anti-FLAG monoclonal antibody M2 (Sigma) or anti-σ70 antibody (BioLegend ...
-
bioRxiv - Plant Biology 2022Quote: For NR activity measurements with the purified protein the procedure was adapted from Sigma quality control tests for product N7265 based on (Gilliam et al. ...
-
bioRxiv - Physiology 2023Quote: ... protein concentrations of pancreatic samples were quantified using a Bradford assay (Sigma-Aldrich, B6916). Insulin and SST values were then normalized to protein concentration ...
-
bioRxiv - Plant Biology 2023Quote: ... The peak fraction of each BE protein was desalted on PD10 desalting column (Sigma) equilibrated in 20 mM Tris/HCl pH 7 ...
-
bioRxiv - Genomics 2022Quote: HC protein fragments were cleaved by thrombin (40 mU/μL) (EMD Millipore, 605157-1KU) overnight at 4 °C ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich, Pool, UK) using bovine serum albumin as standard ...
-
bioRxiv - Molecular Biology 2022Quote: ... Total or nuclear protein was extracted using a nuclei isolation kit (NUC201, Sigma-Aldrich). Protein samples were electrophoresed on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene fluoride (PVDF ...
-
McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... for each IP reaction 50 μl of Protein A agarose suspension (Merck Millipore, IP02) was incubated with 1.5 μg of GFP antibody (mouse anti-GFP ...
-
bioRxiv - Molecular Biology 2022Quote: ... The protein complex was eluted in Strep Buffer supplemented with 2.5 mM desthiobiotin (Sigma) at 1 mL/min ...
-
bioRxiv - Molecular Biology 2022Quote: ... and the proteins were then digested with 10 µg/mL proteinase K (Sigma-Aldrich) at 55 °C overnight ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins on gels were transferred on PVDF membrane (Immobilon-P, Millipore, Cat no. IPVH00010) and blocked with 5% non-fat dry milk (Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: We concentrated recombinant EGFP fusion proteins using Amicon Ultra centrifugal filters (10K MWCO, Millipore) to an appropriate protein concentration in 250 mM NaCl salt buffer ...
-
bioRxiv - Pathology 2022Quote: ... the incubated protein mixture was collected and centrifuged using centrifugal filters Utracel-100K (Millipore) at 500 × g for 10 min ...
-
bioRxiv - Plant Biology 2022Quote: ... The total ubiquitinated proteins were detected by anti-FLAG (F-3165, 1:5,000, Sigma) and α-MYC (ab32 ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were resolved by and blotted on Transfer membrane (Immobilon-P, Merck Millipore IPVH00010). Membrane blocking (5% BSA/TBS ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (EMD Millipore, catalog no. WBKLS0500) and ChemiDoc Imaging System (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich, Germany).
-
bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
-
bioRxiv - Plant Biology 2024Quote: ... The separated proteins were transferred to Immobilon®-P PVDF membrane (Merck Millipore, USA) and probed with primary antibodies overnight ...
-
bioRxiv - Microbiology 2024Quote: Incubation mixtures (30 µl) contained 10 µM of protein and 5× SYPRO Orange (Sigma) in 25 mM Tris-HCl pH 8.0 ...