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Citations for Millipore Sigma :
3651 - 3700 of 7599 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... The antibody was captured using 30 μl of 50% Protein G Sepharose beads (Sigma) for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Microbiology 2019Quote: ... and the soluble proteins were separated from the OMVs using an iodixanol (Optiprep, Sigma) density gradient ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies used were rabbit anti-prosurfactant protein C (SPC) (Millipore, AB3786, 1:500) and goat anti-IL-33 (R&D ...
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bioRxiv - Microbiology 2019Quote: ... 10 mg of total proteins were mixed with Magnetic anti-FLAG beads (Sigma, USA) and incubated over-night with end-over-end rotation at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2019Quote: ... Protein was concentrated using a centrifugal concentrator with an MWCO below 15 kDa (Millipore). The concentrated protein was sub-aliquoted ...
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bioRxiv - Bioengineering 2020Quote: ... Proteins were then reduced with 10 mM DTT/40 mM NH4HCO3 (Sigma-Aldrich Co.) at 56°C for 20 min ...
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bioRxiv - Genetics 2020Quote: The GST-Clr4 fusion protein was allowed to bind to Glutathione-agarose beads (Sigma) in MTPBS buffer (100mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... The purified VxrB protein was concentrated by centrifugal filter devices (Millipore, 3,000 MW CO) before injection onto High load TM (16/60 ...
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bioRxiv - Cell Biology 2020Quote: ... 3 mg of each sample was precleared with EZview protein G beads (Sigma-Aldrich) for 0.5 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: Nuclear extracts were pre-cleared by incubation with 40 μl Protein A dynabeads (Sigma) for one hour at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Soluble protein was measured using a Bicinchronicinc acid kit (BCA1, Sigma-Aldrich Canada Co.). Absorbance of each reaction was measured in a spectrophotometer (Spectra Max M2 ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were initially cross-linked to DNA with 1% formaldehyde (Sigma, Cat. No F8775) for 15 minutes in 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The collected protein fractions were pooled and concentrated using an Amicon ultrafiltration cell (Millipore) and dialyzed into PBS (10 mM Na2HPO4 ...
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bioRxiv - Biochemistry 2021Quote: Proteins resolved through SDS-PAGE gels were transferred to Immobilon-P PVDF membranes (Millipore) and blocked with 3% non-fat milk in Tris-buffered saline with 0.1% Tween-20 (TBST) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein expression for all constructs was conducted using Rosetta II pLac(I) cells (Millipore) grown to high density at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were ultrafiltrated using 10 K pore size Amicon Ultra filter units (Millipore) and step-wise dialyzed against 5 mM Pipes-Na pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was induced with 0.25 mM IPTG in E.coli Rosetta BL21(DE3) (Novagen) in the presence of 50 µM ZnCl2 at 37° C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Pure protein was concentrated using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa MWCO) to around 20 μM ...
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bioRxiv - Biochemistry 2021Quote: ... Pure protein was concentrated using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa MWCO) to around 20 μM ...
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bioRxiv - Biochemistry 2021Quote: ... Pure protein was concentrated using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa MWCO) to around 55 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 μg of purified protein was mixed with 2 mM of acetyl phosphate (Sigma) in 100 μl of phosphorylation buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... The secreted S proteins were purified by anti-FLAG M2 affinity resin (Sigma Aldrich). After loading two times ...
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bioRxiv - Cell Biology 2021Quote: ... we inhibited global protein synthesis with cycloheximide (CHX, 100 μg/mL, Sigma Aldrich # C4859).
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bioRxiv - Molecular Biology 2021Quote: ... Total proteins were extracted from transfected 293T cells using RIPA lysis buffer (Millipore Sigma) at 48 hpt ...
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bioRxiv - Cancer Biology 2021Quote: ... and 20 mg of protein was mixed with 2x Laemmli buffer (Sigma Aldrich, #S3401) and boiled for 5 min at 95°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then electrotransferred to an Immobilon®-P polyvinylidene fluoride (PVDF) membrane (Millipore). Membrane was blocked with 5% non-fat milk ...
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bioRxiv - Cell Biology 2019Quote: ... the purified protein was concentrated over a 10k Amicon Ultra-4 Centrifugal filter (Millipore) then immediately aliquoted ...
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bioRxiv - Genomics 2021Quote: Proteins were separated using 6-15% SDS-PAGE and transferred onto PVDF membranes (Millipore). Then ...
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bioRxiv - Biophysics 2020Quote: ... The eluted protein was concentrated by an Amicon Ultra filter (100 kDa cutoff, Millipore) and further purified using Superose 6 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: ... Protein transfer was checked by staining the membrane with Ponceau S red (Sigma-Aldrich). The membrane was then blocked using 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Protein pellets were solubilised in 30 μl of 2 M Tris pH 8.0 (Sigma) / 70μl 3X SDS-PAGE loading buffer (60 mM Tris pH 6.8 (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing cleaved proteins was concentrated using a 100K MWCO concentrator (EMD Millipore) to 500 μL and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Genetics 2021Quote: ... Proteins were blotted from the gel onto polyvinylidene difluoride (PVDF) membranes (Immobilon, EMD Millipore) and detected using primary and secondary antibodies ...
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bioRxiv - Immunology 2020Quote: ... and then with protein A agarose/salmon sperm DNA slurry (Millipore, Cat# 16-157). The beads were washed and eluted according to protocols described before (42) ...
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bioRxiv - Microbiology 2021Quote: The proteins in the elution were concentrated by Amicon Ultra centrifugal filters (Millipore Sigma) and separated briefly in a 10% Bis-Tris SDS-PAGE gel for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... The resultant protein bands were visualized after staining with Ponceau-S (Sigma-Aldrich, USA). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... and 500 U/mL ESGRO recombinant mouse Leukemia Inhibitory Factor (LIF) protein (Sigma, ESG1107) at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal antibody directed against Purkinje cell protein 4 (PCP4; Sigma, dilution 1:400), rabbit polyclonal antibody raised to Capn15 (Zha ...
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bioRxiv - Immunology 2020Quote: ... ABCF1 protein was probed with primary anti-ABCF1 antibody (HPA017578, Sigma-Aldrich, Oakville, Ontario) at 1:100 in 3% Casein in 1X Tris Buffered Saline with TWEEN ® 20 (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Purified recombinant S proteins were concentrated using Amicon filtration devices (EMD Millipore, Billerica, MA), buffer-exchanged into PBS and analyzed by SDS-PAGE and Western blotting ...
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bioRxiv - Immunology 2021Quote: ... Excess Protein-PEG2000-DPSE was separated from liposomes using a CL-4B Sepharose (Sigma) column ...
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bioRxiv - Neuroscience 2020Quote: ... Synaptic proteins were detected mouse anti-PSD95 (1:500, MAB1598, Millipore, Burlington, MA, U.S.A.), guinea pig anti-VGLUT1 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Primary and secondary antibodies were diluted as follows: polyclonal rabbit-anti-Protein A (Sigma) detecting the PTP epitope 1:2000 ...
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bioRxiv - Immunology 2021Quote: Actin was used as a housekeeping protein (Rabbit anti-Actin antibody (Sigma-Aldrich A2066) followed by Goat anti-Rabbit-HRP (Southern Biotech 4050-05)).