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Citations for Millipore Sigma :
3701 - 3750 of 10000+ citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Total proteins were extracted from cells with the M-PER Mammalian Protein Extraction Reagent (Sigma, GE28-9412-79) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... Fc chimeric proteins were purified via affinity based Fast Protein Liquid chromatography using Prosep® Ultra resin (Millipore). Fc conjugated proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using Pierce™ BCA Protein Assay Kit (Sigma-Aldrich, St. Louis, Missouri United States) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... solium larval extracts were assessed for protein concentration using a BCA or Bradford protein assay kit (Sigma-Aldrich), respectively.
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bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 4-HNE protein adducts were detected by an anti 4-HNE protein adduct antibody (1:2000, AB5605, Millipore) in Tris-buffer saline with 5% skim milk ...
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bioRxiv - Microbiology 2020Quote: Oxidized proteins containing carbonyl groups were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma-Aldrich: cat#S7150). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... Total protein was measured using the protein extract for PEPCK activity using Bradford reagent (Millipore Sigma; Cat: B6916) and a standard curve using commercial bovine serum albumin standards (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... The protein solutions were subsequently desalted and protein amount was estimated using Direct Detect cards (Merck Millipore, Darmstadt). All samples were freeze dried before further processing ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was diluted into 25μL with 5x concentration SYPRO Orange Protein Gel Stain (Sigma Aldrich) in duplicate ...
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bioRxiv - Neuroscience 2024Quote: ... The recovered protein pellet was further processed for western blot-based analysis and quantitation of TH protein (Millipore, Temecula ...
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bioRxiv - Cancer Biology 2023Quote: ... the protein concentrations were measured using a standard BCA assay with Pierce™ BCA Protein Assay Kit (Sigma). 30 μg of proteins was loaded into a pre-cast gel (Novex™ 16% Tricine Gels ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin pre-clearing was done by incubating with Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) for 4 hrs at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Cell Biology 2022Quote: Recombinant His6-EGFP used in the GFP intensity calibration (Figure 1B) was expressed in Rosetta 2 (DE3) competent cells (Millipore Sigma #71400) using pDual-EGFP plasmid (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: TKO and QKO mESC were cultured in Serum/LIF media (DMEM-KO, 15% FBS, 1000 U/ml murine recombinant LIF (Millipore Sigma #ESG1106), 1× nonessential amino acids (100x ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems) were spotted and used as standards ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Microbiology 2023Quote: ... were transformed with the lithium acetate method 45 and strains containing the recombinant plasmids were selected on YPD agar with 200 μg/mL geneticin (G418 – Sigma A1720-5G).
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bioRxiv - Cell Biology 2023Quote: The custom recombinant antibody-like anti-MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076) (Gibson et al. ...
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bioRxiv - Plant Biology 2020Quote: ... antibodies and Protein A agarose beads (Sigma). After elution of immune complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were blotted onto Immobilon-P (Millipore) with a semi-dry blotting apparatus (TRANS-BLOT SD ...
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bioRxiv - Cancer Biology 2021Quote: ... protein G beads (EMD Millipore, #16-266), biotin (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred to PVDF membrane (Millipore). The membrane was cut into strips corresponding to the molecular weight of proteins of interest ...
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bioRxiv - Cell Biology 2020Quote: ... Polyclonal rabbit-anti-Protein A antibody (Sigma) and monoclonal mouse-anti-TAC102 antibody were used as described above ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with trypsin (Sigma-Aldrich) overnight at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 60μl of protein-A-Sepharose (P9424, Sigma) was added and samples were incubated 3h at 4°C under agitation ...
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bioRxiv - Cell Biology 2020Quote: Protein was isolated in RIPA buffer (Sigma) and subjected to SDS-PAGE as described previously ...
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bioRxiv - Biophysics 2020Quote: ... proteins were transferred to PVDF membranes (Millipore).
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bioRxiv - Developmental Biology 2021Quote: ... Protein Phosphatase 2A1 bovine (Millipore-Sigma, P6993) was used to digest 100 μl total protein extracted from O9-1 CNCCs ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein Phosphatase 2A1 bovine (Millipore-Sigma, P6993) was used to digest 100 μl total protein extracted from O9-1 CNCCs ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein was detected by enhanced chemiluminescence (Millipore).
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were detected by enhanced chemiluminescence (Millipore).
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bioRxiv - Evolutionary Biology 2020Quote: ... protein G-magnetic beads (Merck Millipore, LSKMAGG10) were used instead ...
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bioRxiv - Developmental Biology 2020Quote: ... proteins were transferred onto Immobilon-FL (Millipore) PVDF membrane and processed for immunodetection ...
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bioRxiv - Developmental Biology 2021Quote: ... Cas9 protein and Phenol Red (Sigma P0290) was assembled on ice and incubated at 37°C for 5 minutes to aid Cas9-gRNA ribonucleoprotein complex formation for more efficient mutagenesis ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A sepharose beads (Sigma-Aldrich, P3391). Neutravidin Resin (Piere ...
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bioRxiv - Neuroscience 2021Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, cat ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to PVDF membrane (Millipore) via semi-dry transfer ...
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bioRxiv - Cancer Biology 2019Quote: ... total SR proteins (1;1000, Sigma-Aldrich, #MABE126), phosphorylated histone H3 Serine 10 (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: PureProteome protein A/G magnetic beads (Millipore) were equilibrated with anti-K(NQTGG ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred to PVDF-FL (Millipore), blocked with Odyssey Blocking Buffer (Li-Cor) ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred to PVDF-FL (Millipore), blocked for 1 hour with 5% Nonfat Dry Milk in Laminin Binding Buffer [LBB ...
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bioRxiv - Biochemistry 2020Quote: ... and Cycloheximide (Protein synthesis inhibitor, Sigma, C7698).
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Biochemical alterations precede neurobehavioral deficits in a novel mouse model of Friedreich ataxiabioRxiv - Biochemistry 2021Quote: ... proteins were transferred to PVDF (Millipore, IPVH00010) or Nitrocellulose (Sigma_Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein were transferred to PVDF membranes (Millipore) and blocked with TNET buffer (200 mM Tris–HCl ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.1 % adhesion proteins laminin (Sigma L2020) (see Fig 14) ...
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bioRxiv - Biochemistry 2021Quote: ... MWCO 10 kDA protein concentrator (Merck Millipore) to the final concentration of 9-10 mg/ml.