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Citations for Millipore Sigma :
3651 - 3700 of 10000+ citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... OFC β-endorphin sections were co-labeled with the primary antibody for goat anti parvalbumin (PV+) (1:1000, SIGMA, SAB2500752), a marker for a cortical interneuron subtype ...
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bioRxiv - Immunology 2020Quote: ... Cells were then labeled for 5 hours at 4C in 50 μL HBSS with 5 mg/mL bovine serum albumin (BSA, Sigma) with 10 μg/mL wheat-germ agglutinin (WGA ...
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bioRxiv - Bioengineering 2021Quote: ... particles were first labeled with AlexaFluor™ 568 streptavidin and suspended in PBS containing 2 mg/mL fluorescein isothiocyanate-dextran (500,000 MW, Sigma). For 35 and 55 µm nanovials the same procedure was used except the nanovials were first diluted 10-fold to reduce fraction of aggregates formed.
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bioRxiv - Biochemistry 2019Quote: ... Pure tubulin and BSA were labeled with commercial NHS-conjugated dyes (Cy5 or Alexa568) according to supplier’s instructions (Sigma: GEPA150101). A similar method was used to conjugate biotin to tubulin (EZ-Link™ NHS-PEG4-Biotin ...
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bioRxiv - Developmental Biology 2019Quote: ... Digoxigenin (DIG)-labeled RNA probes were prepared by in vitro transcription with linearized constructs using DIG RNA Labeling Kit (SP6/T7) (Sigma). Embryos were fixed in 4% PFA in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... and the secondary fluorescence-labeled anti-mouse IgG antibody (2.5 µg/mL; goat anti-mouse IgG, Atto647N, Sigma Aldrich, Germany) were mixed in one batch and offered in a volume of 2 µL to the beads from the opposite side ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were labeled in media for 10 minutes with 500 µM 4-thiouridine (4sU, Sigma-Aldrich, St. Louis, MO, USA.). RNA extraction was performed with TRIzol (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: 15N-accumulation assay after 15N-nitrate labeling was performed with 15N-labeled KNO3 (99 atom % 15N, Sigma-Aldrich, no. 335134). For 15N-nitrate accumulation assay ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were stained with methylene blue and hybridized to 32P radio-labeled DNA probes (DecaLabel, ThermoFischer) in PerfectHyb™ (Sigma) over night at 65 ºC ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei were then labeled in a solution of anti-NeuN antibody conjugated to Alexa Fluor 488 (A60, Millipore, 1/1000) and 0.1% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were cultured in complete medium with 1 μg/mL doxycycline for 48 hours labeled with 10 μM EdU (Sigma) for 1 hour prior to harvesting.
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bioRxiv - Molecular Biology 2021Quote: ... The resulting lysates were incubated with a 23-bp customized biotin-labeled DNA substrate containing an O6-methylguanine next to the PstI cleavage site (Sigma) for 2 h at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... after which bone marrow cells were collected and labeled with monoclonal anti-mouse CD3 (Sigma, catalog no. SAB4700050, 1: 1000), CD19 (Sigma ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by incubation with a secondary Cy3-labeled polyclonal goat anti-rabbit antibody (AP132C, 1:800, Merck Millipore, Burlington, USA). Cells were mounted on slides using Fluoroshield™ with DAPI mounting medium (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: Protease assays were performed with a protease fluorescent detection kit using casein labeled with fluorescein isothiocyanate (FITC) as the substrate following the manufacturer’s instructions (PF0100, Sigma-Aldrich). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Effector (TILs) and target cells (autologous tumor cell lines) were labeled respectively with 1μM PKH67 and PKH26 fluorescent dyes (Sigma-Aldrich) according to the manufacturer’s protocol and loaded on the chip array ...
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bioRxiv - Molecular Biology 2022Quote: ... 32P-labeled probes that reverse complement to the targeted miRNAs were hybridized with membrane in PerfectHyb(tm) Plus Hybridization Buffer (Sigma) overnight at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Ltk-/- and DKO mice were labeled in vivo by intraperitoneal injections with BrdU (150 µg/g body weight, Sigma-Aldrich,). In some experiments PPP (20 mg/kg body weight ...
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bioRxiv - Microbiology 2023Quote: ... Parasites attached to coverslips were fixed in 4% paraformaldehyde at room temperature for 20 min and labeled with wheat germ agglutinin (WGA) lectin from Triticum vulgaris conjugated with FITC (Sigma). To this end ...
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bioRxiv - Microbiology 2022Quote: ... The cell membrane and nuclei of the midgut tissue were labeled for 30 min with FM1-43 (5 μg/ml) (Sigma) and DAPI (0.5 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Polar metabolites were resuspended in 170 µL of 100% methanol containing 13C-15N labeled amino acids (30 µM, 767964, Sigma). UHPLC normal phase chromatography was performed using an Agilent 1290 LC stack ...
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bioRxiv - Biophysics 2022Quote: ... 2H2O M9 media containing 1 g/L 15NH4Cl and 0.5 g/L 2H/15N-labeled ISOGRO (Millipore-Sigma, Burlington, MA). In both cases ...
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bioRxiv - Biophysics 2022Quote: ... 2H2O M9 media containing 1 g/L 15NH4Cl and 0.5 g/L 2H/15N-labeled ISOGRO (Millipore-Sigma, Burlington, MA). In both cases ...
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bioRxiv - Immunology 2024Quote: ... Infected erythrocytes were labeled with a membrane dye using the PKH26R Red Fluorescent Cell Linker Midi Kit (Sigma #MIDI26-1KT) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... Fluorecent probe Atto488-DOPE (1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto-488) was purchased from Sigma-Aldrich (international). Sucrose ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 DL of the supernatants containing 32P labeled intracellular nucleotides were resolved on a 20 x 20 cm plastic PEI cellulose F TLC plate (Millipore) using 1.5 M KH2PO4 pH 3.4 as a solvent ...
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bioRxiv - Plant Biology 2023Quote: ... then resuspended in 100% MeOH containing an internal standard mix of isotopically labeled compounds (∼15 µM average of 5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 10 µg/mL 13C-trehalose ...
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bioRxiv - Genetics 2023Quote: ... the isolated cells were characterized by flow cytometry using the SMC-specific FITC-labeled anti-α-SMA antibody (Sigma, F3777) and an isotype control (mouse IgG2a-FITC ...
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bioRxiv - Biophysics 2023Quote: ... The molecules used for the control experiments are TRITC-labeled dextran molecules having a molecular weight of 70 kDa (Model No.T1162) from Sigma Aldrich Chemicals Pvt ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed four to five times with warm media (DME-HG for MEFs and DME-HG/F12 for MCF10A) and pulse-labeled with 100 µM 5- chloro-2’-deoxyuridine (CldU; Sigma) for 20 min at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Precusor nCys- mEpoR peptide was labeled at its free amino N-terminus as a protected peptide on resin with NHS-Biotin (Sigma) by swelling the resin with DMF having 10 equivalents N,N- Diisopropylethylamine ...
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bioRxiv - Biochemistry 2022Quote: ... N-termini were labeled with light and heavy formaldehyde (12CH2O in H2O, and13CD2O in D2O (252549 and 596388, Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2023Quote: ... 12-mer Atto565-labeled or Atto647N-labeled secondary imaging oligos were diluted to 20 nM in hybridization buffer (5% ethylene carbonate (EC, Cat.# E26258, Sigma) in 2XSSC/0.2% tween) ...
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bioRxiv - Biochemistry 2023Quote: Cell lysates from cardiomyocytes treated with 10 or 50 µM H2O2 and controls were labeled with MAL-PEG (5kDa methoxy polyethylene glycol maleimide, Sigma) following a slightly modified version of a previously published procedure (15) ...
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bioRxiv - Developmental Biology 2023Quote: ... the PCR products were used as templates for in vitro transcription of DIG-labeled RNA probe using T7 RNA polymerase (SIGMA) and DIG RNA Labeling Mix (SIGMA ...
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bioRxiv - Molecular Biology 2023Quote: ... Reaction mixes were made with 10 μM bovine tubulin (PurSolutions) containing 5% Alexa 568-labeled bovine tubulin and 1 mg/mL BSA (Sigma) in assay buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The vascular lumen was labeled by the fluorescent dye rhodamine B-dextran (Wt∼70kD) (Sigma, Cat#R9379, 10 mg/ml) through tail intravenous injection ...
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bioRxiv - Biochemistry 2023Quote: ... A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich), 0.2 mg/ml glucose oxidase (Merck) ...
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bioRxiv - Cancer Biology 2023Quote: SCID mice bearing tumors generated by MDA-MB-231/Dendra2 cells at size of 1.3 cm in diameter were injected with tetramethylrhodamine-labeled 155kD dextran (Sigma-Aldrich) ten minutes before imaging ...
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bioRxiv - Neuroscience 2023Quote: Gut permeability was measured using oral administration of fluorescein-isothiocyanate-labeled dextran (FITC-dextran; molecular weight 4kDa; Sigma- Aldrich 46944). Adult male and female rats (P60 ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa 488-labeled phalloidin was dissolved in methanol to yield a 40X 6.6 μM stock solution whereas DAPI dihydrochloride (Sigma-Aldrich) was dissolved in UPW to yield a 100X 250 μG mL-1 stock solution ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies were rinsed with PBS and nuclei were labeled using DAPI (4’6-diamidino-2-phenylindole; Sigma, St. Louis, MS) in PBS (1:10,000 of 3mg/mL DAPI stock ...
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bioRxiv - Developmental Biology 2024Quote: ... Kidneys were then stained with 20 μg ml-1 AlexaFluor 488-labeled peanut (Arachis hypogaea) agglutinin lectin (PNA, L21409, Sigma) for 1 hr in RNAse-free PBS to mark ureteric bud tubules ...
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bioRxiv - Biophysics 2024Quote: ... Microtubules were labeled with anti-tubulin antibodies 1:200 in PBS (Monoclonal Anti-α-Tubulin antibody; Sigma-Aldrich, Poznań, Poland) for 24 hours and stained with anti-mouse antibodies conjugated with Alexa Fluor 555 (Goat anti-Mouse IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were labeled with a rabbit anti-KIF5B antibody (Tanaka et al., 1998) and a mouse anti-insulin antibody (Sigma), followed by Alexa-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: To visualize projection neurons labeled by BDA we incubated free-floating sections for 1 h at 4°C in 0.05M glycine (Sigma-Aldrich) and preblocked for 1 h at 4°C in 5% normal goat serum (NGS ...
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bioRxiv - Microbiology 2024Quote: ... Each extract was resuspended in 170 µL of 100% methanol containing isotopically labeled internal standards (5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 1 ug/mL 2-amino-3-bromo-5-methylbenzoic acid ...
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bioRxiv - Plant Biology 2020Quote: ... The protein amount of the resuspended membrane fractions was quantified by Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) and equilibrated between different samples ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein was measured using the protein extract for PEPCK activity using Bradford reagent (Millipore Sigma; Cat: B6916) and commercial bovine serum albumin standards (Thermo Fisher 23208) ...