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Citations for Millipore Sigma :
3851 - 3900 of 10000+ citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... were captured using 40 μl of protein-A or protein-G Dynabeads (Invitrogen; pre-washed with 0.1% BSA [Fraction V, Sigma), by 4 hrs incubation time at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Protein-protein interaction was measured by PLA with Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92102, Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and protein was estimated using the bicinchoninic acid protein estimation method (BCA, B9643, Sigma-Aldrich). We separated a protein sample (80µg ...
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bioRxiv - Paleontology 2023Quote: Information for the protein standards purchased for the ToF-SIMS analyses are as follows: type-1 collagen protein (Sigma, CAS ...
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bioRxiv - Cell Biology 2024Quote: ... Yeast protein extracts were separated on protein gels containing 10% polyacrylamide and transferred to an Immobilon-P membrane (Millipore). The membranes were probed with anti-Myc antibodies (9E10 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were then labeled with the PLA antimouse PLUS probe (DUO92001) and the PLA anti-rabbit MINUS probe (DUO92005) purchased from Sigma-Aldrich. Detection was performed with Duolink in situ detection reagents red kit (DUO92008 ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or fluorescein-labeled riboprobes were synthesized as described (Pearson et al., 2009) and detected with anti-digoxigenin-HRP (1:2000, Roche/Sigma-Aldrich 11207733910 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were fluorescently labeled using methyl green (Prieto et al, 2014) and actin filaments with TRITC-conjugated phalloidin (Sigma-Aldrich, P1951). Observation of whole embryos was performed using a Zeiss LSM 880 or a Zeiss LSM 800 laser confocal microscope ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were rinsed 6 times in 1x PBS before being incubated with fluorescence-labeled secondary antibody (diluted in blocking buffer at 1:500 dilution ratio with 1/10000 Hoechst 33258, Sigma #94403) for 1 hour ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL of DIG labeled probe was diluted in 100 μL prehybridization buffer containing 400 ng tRNA (Sigma-Aldrich, St. Louis) and denatured at 70°C for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... flat 1:30 PDMS stamps were incubated for 1 h with 40 mL of 50 mg/mL Alexa Fluor 647–labeled and 50 mg/mL unlabeled fibronectin (F1141; Sigma-Aldrich), then washed and dried ...
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bioRxiv - Biochemistry 2020Quote: Rhodamine-labeled microtubules were polymerized from bovine tubulin (~10% final rhodamine-labeled tubulin, prepared as previously and stabilized with 10 μM taxol (Sigma T7191) before being pelleted in a TLA120.1 rotor at 90,000 rpm for 10 min at 25°C (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... Then the particles were re-suspended in 50 mM pH 7.5 HEPES buffer, labeled with self-healing Cy3 and Cy5 derivatives (LD555-CDand LD650-CoA, respectively) and purified through an OptiprepTM (Sigma Aldrich) gradient as previously described (Lu et al. ...
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bioRxiv - Cell Biology 2021Quote: ... 100 nM of the specified SPR2-GFP proteins along with 2.5 μM 1:9 rhodamine-labeled porcine tubulin were then introduced into the flow chamber in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrin), 50 mM DTT and an oxygen-scavenging system consisting of 250 μg/ml glucose oxidase ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule polymerization was initiated by flowing in 20 mM 1:25 rhodamine-labeled porcine tubulin in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrich), 50 mM DTT ...
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bioRxiv - Cell Biology 2021Quote: ... The blot was then neutralized with SSC and hybridized with a DIG-labeled telomere probe prepared as described 48 in DIG Easy Hyb (Sigma-Aldrich) solution overnight at 42°C ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were washed in DPBS (5 minutes each wash) and incubated with 2.5 µg/ml an FITC-labeled anti-HA antibody (Sigma, Product # H7411) diluted with 5% BSA in DPBS for 1.5 h at room temperature ...
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Stress response in Entamoeba histolytica is associated with robust processing of tRNA to tRNA-halvesbioRxiv - Microbiology 2021Quote: ... Probe DNA was 5′-end labeled by PNK reaction using γ-[32P]-ATP and hybridized with the membrane in perfectHyb buffer (Sigma) overnight at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... The FISH hybridization mixture (20 ng of the labeled probe, 10 μg of salmon sperm, 50% of deionized formamide (Sigma-Aldrich)) in 2 x SSC was incubated on slides at 82 °C for 5 min ...
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bioRxiv - Immunology 2022Quote: ... CHO CoV-2-Spike cells were used as target cells and were fluorescently labeled with PKH67 Fluorescent Cell Linker Kit (Sigma Aldrich) prior to incubation with mAbs ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde and stained with mitochondrial networks labeled with a primary antibody against TOMM20 (Sigma-Aldrich, HPA011562) used at 1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-sense DIG-labeled in-situ probes were generated using linearized plasmid cDNA templates with 10X DIG RNA labeling mix (Sigma 11277073910) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-sense DIG labeled in- situ probes were generated using linearized plasmid cDNA templates with the10X DIG RNA labeling mix (Sigma 11277073910) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: 5′end biotin-labeled TP53 3′UTR RNA fragments with different PAS and other mutations and miRNA of interest labeled with biotin at the 3′end were commercially synthesized (Sigma-Aldrich). H1299 cells were seeded one day before transfection in 10 cm tissue culture dish at 50% confluent ...
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bioRxiv - Molecular Biology 2019Quote: ... 3.6 x 107 cells in growth medium were labeled at a final concentration of 500 µM 4-thio-uracil (4sU, Sigma-Aldrich), and incubated at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... 5 × 106 activated B cells were washed with PBS and re-cultured in glucose-free medium RPMI medium containing 10% dialyzed FBS and uniformly labeled [13C]-glucose (2 g/L; Sigma-Aldrich) for 24 and 48 h ...
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bioRxiv - Neuroscience 2019Quote: ... then labeled with a blend of three FITC-conjugated anti-nucleoprotein monoclonal antibodies (Light Diagnostics Rabies DFA Reagent, EMD Millipore 5100) diluted 1:100 in blocking buffer for 30 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... and combinations of the following lysine and arginine isotopologues: for “light” (“L”)–labeled cells L-arginine (84 mg/L) and L-lysine (146 mg/L)(Sigma-Aldrich); for “medium” (“M”)-labeled cells ...
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bioRxiv - Biochemistry 2021Quote: ... intact histones were mixed with 5 μL of deuterium-labeled propionic anhydride (propionic anhydride-d10) rapidly followed by 14 μL of ammonium hydroxide (Sigma-Aldrich) to adjust to pH 8.0 ...
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bioRxiv - Genetics 2021Quote: ... Some samples were labeled only with the mouse monoclonal DM 1A anti-a-tubulin (#T9026) from Sigma-Aldrich (St-Louis, MO), diluted 1:500 ...
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bioRxiv - Immunology 2021Quote: ... pBF2*1501 epitope-specific tetramers were prepared by mixing the biotinylated pBF2*1501 complexes and phycoerythrin (PE)-labeled streptavidin (Sigma Aldrich) at a molar ratio of 4:1 ...
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bioRxiv - Cancer Biology 2022Quote: ... click chemistry reactions were performed on pre-permeabilized samples to conjugate biotin to the EdU-labeled DNA by using Biotin Picolyl azide (Sigma Aldrich). Sonication was performed with a Bioruptor Pico sonicator (Diagenode ...
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bioRxiv - Biophysics 2022Quote: ... FLAG- TOP3A-RMI1/2 or FLAG-DNA2 were labeled with QDs pre-incubated with a mouse anti-FLAG antibody (Sigma-Aldrich) on ice for 10 minutes prior to injection ...
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bioRxiv - Biophysics 2022Quote: GMPCPP-stabilized microtubule seeds polymerized from biotinylated and TAMRA-labeled bovine tubulin were attached to the surface of a microchannel using anti-biotin antibodies (Sigma-Aldrich). Microtubules were assembled using 12 μM unlabeled bovine GTP-tubulin for 10 min at 28 °C in polymerization buffer (80 mM K-PIPES ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then washed with PBS-T and incubated with anti-rabbit or anti-mouse peroxidase-labeled antibodies (1:10000, Sigma-Aldrich). After extensive washing with PBS-T ...
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bioRxiv - Zoology 2023Quote: ... tissues were hybridized with digoxigenin labeled antisense DNA and subsequently exposed to anti-digoxigenin Fab’ fragments conjugated to alkaline phosphatase (Sigma-Aldrich) and peptide primary antisera ...
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bioRxiv - Immunology 2024Quote: ... the trypsin was used as is and the pepsin one was labeled at the C-terminal end using Arginine methyl ester dihydrochloride (Sigma-Aldrich) using the method used in (36).
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bioRxiv - Developmental Biology 2023Quote: ... Full lengthrfx6.L and pdx1.L anti-sense DIG-labeled in-situ probes were generated using linearized plasmid cDNA templates with 10× DIG RNA labeling mix (Sigma 11277073910) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2023Quote: Replicating cells and nascent mtDNA were labeled by administering a dose of 100 mg/kg body weight BrdU (Sigma-Aldrich, #B5002) via intraperitoneal injection to P28-29 mice ...
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bioRxiv - Cancer Biology 2023Quote: ... The grid was subsequently washed with MQ water followed by incubation for 20 min with 10 μl of anti-mouse 10 nm gold-labeled secondary antibody (1:10) (Sigma-Aldrich). Further ...
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bioRxiv - Cell Biology 2023Quote: ... The washed nuclei pellets were resuspended in sorting buffer (2% BSA/RNase-free PBS) and labeled with anti-PCM1 (1:500, HPA023374, Sigma-Aldrich) on ice for 45 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde and stained with mitochondrial networks labeled with a primary antibody against TOMM20 (Sigma-Aldrich, HPA011562) used at 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... then labeled with a 1:100 dilution of anti-nucleoprotein monoclonal antibody blend (Light Diagnostics Rabies DFA Reagent, Millipore Sigma 5100) as well as a 1:250 dilution of rabbit anti-Cre polyclonal antibody (Millipore Sigma 69050 ...
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bioRxiv - Biochemistry 2022Quote: EMSAs for the SAP-domain were performed with DNA such that the single strand or one of the two strands in dsDNA was 5’-labeled with fluorescein (6-FAM, Sigma-Aldrich) (Table 1) ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-fluorescein-AP Fab fragment antibody (cat N° 11426338910) for the detection of fluorescein-labeled lectin was obtained from Sigma-Aldrich. FITC-Concanavalin A lectin was purchased from Vector Laboratories ...
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bioRxiv - Plant Biology 2023Quote: Proteasome activity was measured by spectrofluorometry using the 7-amino-4-methylcoumarin (AMC)-labeled fluorogenic substrate succinate-LLVY-AMC (Roche Sigma-Aldrich) in cleared extracts of leaf 1 at different dpg (Üstün and Börnke ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were labeled with 4’,6-Diamidino-2-Phenylindole (DAPI) and slides were mounted using Fluoromount mounting medium (Sigma, cat. # F4680).
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bioRxiv - Cancer Biology 2023Quote: All cell lines subjected to this analysis were grown asynchronously and labeled with 30 μM of the thymidine analog chlorodeoxyuridine (CldU; Sigma-Aldrich) for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded on a 18×24 mm coverslip and the next day they were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Biophysics 2023Quote: ... the cell was infused with 0.5 nM BfiI and 0.2 nM of Cy3B-labeled dsDNA oligonucleotide in imaging buffer (IB, the YB supplemented with 15 units/mL of glucose oxidase (G6125, Sigma-Aldrich), 1% of glucose (G0047 ...