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Citations for Millipore Sigma :
3701 - 3750 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Samples were stained for DAPI diluted in water for 2 min and washed 3 times with water before drying cells on poly-L-lysine (Sigma, P8920) coated coverslips at room temperature protected from light ...
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bioRxiv - Cancer Biology 2022Quote: ... Mice were induced to form tumors at 2-3 months of age by administration of tamoxifen (Sigma-Aldrich, St. Louis, MO) using 100 mg/kg (in corn oil ...
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bioRxiv - Cell Biology 2022Quote: ... Cells from each donor animal were plated as separate cultures in serum-free growth medium42 (SFGM, Supplementary Table 3) on fibronectin-coated (4 μg cm−2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels for 72 h prior to FACS ...
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bioRxiv - Microbiology 2020Quote: ... were passaged 2-3 times weekly and grown at 37° C with 5% CO2 in DMEM containing 10 mM L-glutamine (Sigma, M8537), supplemented with 10% heat-inactivated FBS (HyClone) ...
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bioRxiv - Microbiology 2021Quote: ... or the same isosmotic solution with 100 µM of the general anion channel inhibitor 5-Nitro-2-(3-phenylpropylamino) benzoic acid (NPPB, Sigma-Aldrich), which inhibits malarial new permeation pathways (59) ...
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bioRxiv - Cancer Biology 2021Quote: ... targeting EZH2 (shEZH2#3 [TRCN0000040076] and shEZH2#4 [TRCN0000010475]) and FAK (shFAK#2 [TRCN0000121211], shFAK#3 [TRCN0000121318], and shFAK#21 [TRCN0000121321]) were purchased from Sigma-Aldrich. pRK5-TGFβRI-FLAG plasmid (#14833 ...
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bioRxiv - Developmental Biology 2021Quote: ... stage embryos were isolated from oviducts in M2 media (pre-warmed at 37°C for at least 2-3 hours) and thereafter cultured in KSOM (Sigma-Aldrich, EmbryoMax KSOM Mouse Embryo Media ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were then washed 3X with EGM-2 medium and re-suspended in PBS containing 3 mg/ml of fibrinogen (Sigma, #F8630) and 0.15 U/ml of aprotinin (Sigma ...
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bioRxiv - Microbiology 2022Quote: Seventy-two hour post-fertilised larvae were first anaesthetized for 2 - 3 min in embryo medium E3 containing 0.2 mg/mL Tricaine (Sigma-Aldrich, USA). After which ...
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bioRxiv - Microbiology 2023Quote: Cross-linking experiments were performed by incubation of 2 mM and 5 mM of suberic-bis-acid ester (3-sulfo-N-hydroxyuccinimy (BS3, Sigma-Aldrich) with the purified recombinant protein in 25 mM sodium phosphate pH 7.4 for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... culturing in liquid media used 10 mL of a mixture of a 2:3 ratio of Todd-Hewitt broth (Sigma-Aldrich) with 0.5% yeast extract (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... Glass coverslips were plasma activated and incubated with a solution of 2% (v/v) 3-(trimethoxysilyl)propyl methacrylate (440159-500ML, Sigma Aldrich) and 1% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... samples were treated for 2 h at room temperature with 3% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA), and 0.3% PBS-T as a blocking agent ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained with freshly made Oil Red O solution (2 parts Milli-Q and 3 parts Oil Red O, Sigma Aldrich). Osteocytes were fixed with ice-cold 70% ethanol at 4°C and stained with freshly prepared supernatant of saturated Alizarin Red S (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were next incubated in 3 mL DME/F-12 medium with 2 units/mL DNase I (Sigma-Aldrich, D4263) at room temperature for 3 min and centrifuged at 450 g for 10 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each well was then topped with 120 µL of PB(2×)M9 (various glucose concentration) and 10 µL of fluorescein (FITC)dextran (3 kDa, anionic, Sigma-Aldrich) solution in PBS to a total volume of 240 µL ...
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bioRxiv - Cell Biology 2023Quote: ... twice with wash buffer 3 (20 mM Tris-HCl pH 7.5, 2 M NaCl and 0.5% IGEPAL CA-630 [Sigma-Aldrich, cat#I8896]), and twice with wash buffer 4 (50 mM Tris-HCl pH 7.5) ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysates were sonicated on ice with a microtip sonicator (120 W, 8 s on, 2 s off, 3 pulses) and supplemented with 200 U of benzonase (Sigma; E8263). The lysates were cleared by centrifugation (18000 g ...
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bioRxiv - Immunology 2022Quote: ... After washed with PBS for 3 times and stained with DAPI (4’,6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... expressing HUVECs (GFP, Olaf Pharmaceuticals, Worcester, MA, USA) were expanded in EGM-2 with 3% extra FBS (FBS, BCBV7611, Sigma-Aldrich) to passage 8 ...
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bioRxiv - Neuroscience 2024Quote: ... Cre-mediated recombination was induced in mice 3-7 months of age by intraperitoneal injection of 2 mg/day tamoxifen (Sigma-Aldrich) diluted with 300 μl corn oil (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were harvested and enzymatically dissociated into a single-cell suspension in PBS supplemented with 3% fetal bovine serum (#FB-1101, Biosera) and 2 mM EDTA (#ED2SS, Sigma=:Aldrich). After 15 minutes of incubation with 5 nM SYTOX™ Red Dead Cell Stain (#S34859 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% TritonX100 in 2 × SSC), anti-bleaching buffer (50 mM Tris-HCl pH 8, 300 mM NaCl, 3 mM Trolox (Sigma 238813), 0.8% D-glucose (Nacalai Tesque 168060-25) ...
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bioRxiv - Plant Biology 2023Quote: ... Flash-frozen plant samples (0.2 g) were ground and extracted using 2 mL of 3 % 5-sulfosalicylic acid (Sigma, ON, Canada). The extract was centrifuged for 10 min at 8050 x g at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... An anti-bleaching buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 2×SSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% D-(+)-Glucose (Nacalai Tesque ...
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bioRxiv - Cancer Biology 2023Quote: Dissected tumors were washed in PBS and homogenized at room temperature in urea lysis buffer (8 M urea, 40 mM Tris pH 7.6, 5% SDS supplemented with phosphatase inhibitor cocktails 2, 3 (Sigma P5726, P0044)) ...
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bioRxiv - Microbiology 2022Quote: ... the medium was supplemented with a mixture of 3 branched-chain fatty acid precursors (100 μm of 2-methyl-butyrate, isobutyrate and isovalerate, Sigma-Aldrich) or straight fatty acid precursors (100 μm of methyl-butyrate ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT, 0.5 mg/ml in PBS, Sigma-Aldrich, USA) with 1 μM of menadione (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 3 times in cold phosphate buffer and postfixed with a solution of 2% osmium tetroxide (#75632 Sigma-Aldrich) and 1.5 % potassium ferrocyanide (P3289 Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue sample was trimmed to 2×3×0.2 mm3 and scanned with a multi-beam serial-section scanning electron microscope (Sigma, Carl Zeiss) in 4×4 nm2 pixels and 33 nm slice thickness ...
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bioRxiv - Microbiology 2024Quote: ... respectively) by dilution in a vehicle solution of 10% w/v 2-hydroxypropyl-!3-cyclodextrin (Sigma-Aldrich, St-Louis, MO, USA) in PBS ...
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bioRxiv - Cancer Biology 2024Quote: Tumours and control tissues (comparable anatomical region) were harvested and dissociated using a hand-held tissue homogenizer (Omni International Inc.) in 2-3 ml RNAzol (Sigma, 4533). Total RNA was isolated using RNAzol ...
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bioRxiv - Microbiology 2024Quote: ... The remaining RBC pellet was suspended in 2 mL of incomplete media and then gently layered on to a 3 mL aliquot of percoll (Sigma Aldrich) diluted with 1X RPMI (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were then incubated for 2-3 hours at 4°C with either 5 μl of anti-FLAG-M2 affinity resin (Sigma-Aldrich), Streptactin sepharose (IBA Lifesciences) ...
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bioRxiv - Biochemistry 2024Quote: ... 1% Triton X-100] and incubated for 2-3 hours at 4°C with either 5 μl of anti-FLAG-M2 affinity resin (Sigma-Aldrich), Streptactin sepharose (IBA Lifesciences) ...
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bioRxiv - Microbiology 2024Quote: ... Larvae of indeterminate sex were infected by injection of 10nL into the caudal vein at 3 days post fertilization (dpf) using a capillary needle containing bacteria diluted in PBS + 2% phenol red (Sigma #P3532), as previously described [104] ...
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bioRxiv - Microbiology 2024Quote: ... Measurement of hexosamines (amino sugars) from the deacetylated samples was done by treatment with 3-methyl-2-benzothiazolone hydrazine hydrochloride (MBTH, Sigma-Aldrich) under mildly acidic conditions ...
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bioRxiv - Immunology 2024Quote: ... a subset of the mice (n = 3-4 per group) received intraperitoneal (i.p.) injections of 2 mg/kg WFA (Millipore-Sigma, #681535), or equivalent volume of the vehicle (VC ...
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bioRxiv - Synthetic Biology 2024Quote: ... All the reagents were prepared in enough amounts for all the experiments for the 2 systems of colicin M and E1 except for the 3-PGA (Sigma Aldrich) was used for colicin M while creatine phosphate (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with TBS-T (TBS + 0.05% Tween20) (3 times x 2 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Plain media was added overnight and on day 3 cells were passaged into selection media containing 2 µg/uL puromycin (Sigma P8833) and 150 µg/mL hygromycin B (Thermo 10687010 ...
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bioRxiv - Cancer Biology 2024Quote: Spheroids grown in collagen for 3/6 days underwent collagen digestion by incubation in DMEM without serum supplemented with 2 mg/ml collagenase (Sigma, C2139) for 45 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Osteogenic differentiation was performed in osteogenic medium for 2 weeks (medium was changed every 3 days) and quantified by alizarin red S staining (Sigma, A5533). The osteogenic differentiation medium contained DMEM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... SIGMA, #M9942, 1:200), (ki67, BD Pharmingen, #550609, 1:200), (C-caspase 3, Cell-signaling, #9661S, 1:100) (PCNT, SIGMA, #HPA016820, 1:200), (P-Histone H3 ...
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bioRxiv - Cancer Biology 2024Quote: ... After 1 hour blocking (3% donkey serum, 1% BSA, 0.3% Triton X-100, PBS) the H3K9me3 antibody (1:500, Millipore, Cat#07-442) or H3K27me3 antibody (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... glass-bottomed dishes were first cleaned and activated with 1% v/v (3-Aminopropyl) triethoxysilane (APTES, Sigma-Aldrich) in ethanol ...
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bioRxiv - Neuroscience 2021Quote: ... 3 ml of an enzyme cocktail [1 mg/ml collagenase A (#11088793001, Sigma-Aldrich, St. Louis, MO, USA) and 0.1 mg/ml elastase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... shShank3 injected mice were randomly infused with 2µL (500nL m-1) of vehicle (∼3 % dimethyl sulfoxide (DMSO, Sigma) diluted in aCSF ...
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bioRxiv - Plant Biology 2020Quote: ... low-fat dry milk) or anti-GFP (Sigma-Aldrich, dilution 1:1000 in TBS-T with 3% BSA) primary antibody at 4°C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... 250 μM or 1 mM L-glutamic acid monosodium salt monohydrate (Sigma Aldrich, cat No. 6106-04-3). Plants were analyzed in single blinded-experiments ...