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Citations for Millipore Sigma :
3801 - 3850 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in urea buffer (50mM Tris HCl pH 7.5, 5mM EDTA, 6M Urea, 1% SDS, 1mM PMSF, 1:100 Phosphatase Inhibitor Cocktail 3 [Sigma-Aldrich P0044]) and cells were lysed by bead beating ...
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bioRxiv - Neuroscience 2021Quote: ... plus 5% normal goat serum for 1 hr at RT before 3 d incubation in primary antibody diluted in block (1:1,000, chicken anti-GFP, Abcam, ab13970; 1:200 rabbit anti-cleaved caspase 3, EMD Millipore, AB3623). Sections were washed several times in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue samples from brain and peripheral organs were homogenized in 3:1 (v/w) of PBS containing 1% Triton X-100 supplemented with protease inhibitors (cOmplete™, Sigma) using the TissueRuptor (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... Cleaned tissue samples were incubated at 37 °C for 1 h in 10 mL DPBS with 1 mM DTT and 3 mM EDTA (Sigma-Aldrich). After incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... pelleted, and re-suspended in lysis buffer (1 mM CaCl2, 3 mM MgCl, 1mM EDTA, 1% Triton X-100 (Sigma-Aldrich), 10 mM TrisHCl ...
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bioRxiv - Bioengineering 2022Quote: ... A stock solution of 3 mg/mL rat tail collagen type I was neutralized with 1% 1 M NaOH (Sigma, S5761) and 10% 10x phosphate buffered saline ...
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bioRxiv - Microbiology 2024Quote: ... parasite pellets were placed on ice for 5 min before addition of 50 μl chloroform followed by 200 μl methanol:ultrapure water (3:1, including scyllo inositol as an internal standard, 1 nmol, Sigma-Aldrich, I8132). Extraction was facilitated through vigorous vortexing ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 1% BSA, 1 mM DTT, 0.1 U/µl Sigma Protector RNase Inhibitor) was then added to the cell lysis and the nuclei were pelleted by centrifuging at 500xg for 5 minutes at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... 6 M Urea and 1% Sodium Dodecyl Sulphate supplemented with 100mM phenylmethylsulfonyl fluoride and 1% phosphatase inhibitor cocktail 3 (Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with fresh protease inhibitors (3 µg/mL Leupeptin, 1 µg/mL macroglobulin, 1 µM benzamidine, 0.5 µM PMSF) and phosphatase Inhibitors (EMD Millipore, 524625). Cell lysates were incubated 10 minutes on ice and centrifuged at 13000 rpm for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... The cells were maintained with DMEM+10%FCS for 3 hours and fixed with acetone/methanol (1:1 ratio) and stained with Giemsa stain (Sigma-Aldrich). Stained cell images were taken by EVOS XL imaging system (Life Technologies).
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bioRxiv - Biochemistry 2024Quote: ... 2% H2O (MilliQ) and were shaken for 3 h before precipitation with 45 mL of ice cold 1:1 diethyl ether (Sigma-Aldrich) and hexanes (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... 2 μg each Spike and Nucleocapsid R203M mutant expression vectors [29,30] using X-tremeGENE 9 DNA Transfection Reagent at 1:3 ratio in serum-free Optimem and 1 hour incubation at room temperature (Millipore Sigma). The supernatant was replaced 4-6 hours post-transfection with 1 mL fresh medium ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by dilution of the supernatant with fresh medium in a ratio of 1:1 and addition of 3 µg/mL hexadimethrine bromide (polybrene, Sigma-Aldrich). 5 µg/mL Puromycin were added and maintained to select for positively transduced cells ...
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bioRxiv - Biochemistry 2024Quote: ... were homogenized using a Polytron in 1 ml of 20 mM sodium acetate buffer containing 0.15 M NaCl and 1% Zwittergent 3-14 detergent (Millipore-Sigma 693017), pH 4.75 ...
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bioRxiv - Synthetic Biology 2024Quote: ... filtered spent media collected from a confluent flask) at the ratio of 1:3 to fresh medium with 25% FBS and 1% penicillin-streptomycin (Penicillin-Streptomycin, Sigma Aldrich). Confluent wells were then assessed with fluorescence microscopes ...
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bioRxiv - Immunology 2024Quote: ... and the second block was for 1 hr with 1× TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... The anti-TXNIP (VDUP1) (mouse mAb, MBL, K0205-3 1:1000) and anti-beta actin (rabbit, Sigma Aldrich, A2066, 1:5000) antibodies were used for target protein detection ...
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bioRxiv - Cancer Biology 2024Quote: ... medium supplemented with 10 mM of 2-(4-(2-hydroxyethyl) piperazin-1-yl) ethane sulfonic acid (HEPES; Sigma-Aldrich, St. Louis, MO), 24 mM sodium bicarbonate ...
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bioRxiv - Biophysics 2021Quote: ... Ultrapure water from a Direct-Q 3 UV water purification system (Millipore, USA) was used for all experiments.
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bioRxiv - Cell Biology 2020Quote: ... and 3 μM CHIR99021 on 0.1% gelatin (Catalog No. ES-006-B, Millipore)-coated plates (for the 2i condition) ...
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bioRxiv - Cell Biology 2020Quote: ... bafilomycin A1 and 3-methyladenine were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich), an equivalent volume of DMSO was added to control samples.
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The plates were washed 3 times with 0.05% Tween-20 (P2287, Sigma-Aldrich) in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... The non-specific binding of antibodies was blocked with 3% BSA (Sigma-Aldrich) for 45 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... (1.5% CMC (3% (w/v) carboxymethylcellulose solution in sterile distilled water (Sigma C4888)) ...
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bioRxiv - Developmental Biology 2020Quote: ... 3-5 colonies were picked and incubated in 2mL Terrific Broth (T0918, Sigma) containing 100µg/mL Ampicillin for 8 hours ...
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bioRxiv - Genomics 2020Quote: ... before addition of 500 μM indole-3-acetic acid solution (“auxin”, Sigma-Aldrich) for different times to induce RPB1 degradation ...
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bioRxiv - Biochemistry 2022Quote: ... or −3 human cDNA into a p3XFLAG-CMV-14 expression vector (Sigma, E7908). Then 3XFLAG-PRLs were cloned into pLenti-CMV-puro (Addgene 17452 ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with 100 μg/ml of 3×FLAG peptide (Sigma). The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min and were blocked with 3% bovine serum albumin (BSA; Sigma) for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then washed with PBS containing 3% Bovine Serum Albumin (BSA, Sigma). Following MeTAFlow assay the cells were passed through a strainer cap to achieve a single cell suspension and immediately analyzed using BD FACS Aria Fusion SORP Cell Sorter ...
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bioRxiv - Cell Biology 2022Quote: ... and 5-Bromo-4-chloro-3-indolyl phosphate-toluoidine were from Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2022Quote: ... Elution was achieved by using 250 μg/ml 3 × Flag peptides (F4799, Sigma). The resulting eluates were resuspended in TRIzol for the following RNA extraction ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All fish were euthanized with ethyl 3-aminobenzoate methanesulfonate (MS-222, Sigma-Aldrich), and then fixed in ethanol or RNAlater solution (Thermo Fisher) ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... they were washed and blocked with 3% Bovine Serum Albumin (BSA, Sigma-Aldrich) and 100 μM NiSO4 for 20 min ...
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bioRxiv - Genomics 2020Quote: ... To the XRN1-AID strain 0.2mM of auxin (3-Indoleacetic acid; Sigma-Aldrich) was added to the media 30 and 60 minutes before harvesting ...
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bioRxiv - Immunology 2021Quote: ... Spleens were gently meshed in FACs buffer (PBS containing 3% FCS from Sigma) using a 40μM cell strainer to prepare single cell suspensions ...
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bioRxiv - Bioengineering 2021Quote: ... Passages were performed by a 3 min treatment of Dispase II (D4693, Sigma) at 37°C ...
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bioRxiv - Genetics 2020Quote: ... for 30min and were incubated with blocking solution [3% bovine serum albumin (Sigma), 5% donkey serum (Millipore) ...
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bioRxiv - Genomics 2021Quote: ... embryos were anesthetized with 500 μM tricaine (Ethyl 3-aminobenzoate methanesulfonate; Sigma-Aldrich), mounted in 3% methyl-cellulose on a glass slide and imaged on a Nikon SMZ25 stereomicroscope.
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bioRxiv - Neuroscience 2020Quote: ... frogs were anesthetized in 0.25% ethyl 3-aminobenzoate methanesulfonate solution (MS222; Sigma-Aldrich); bags of ovaries were removed surgically from the anesthetized frogs ...
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bioRxiv - Neuroscience 2020Quote: ... 3% agar gel consisting of 10% concentration fluorescein dye (F2456, Sigma Aldrich Inc.) was prepared ...
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bioRxiv - Microbiology 2021Quote: ... 3 ml LB top-agar (LTA: 20g LB, Sigma; 7.5 g agar, Formedium) or BHI top-agar (BTA ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were then incubated with 3% (v/v) hydrogen peroxide solution (Sigma-Aldrich) for 20 min to block endo-peroxydase activity ...
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bioRxiv - Neuroscience 2020Quote: ... Permeabilization and blocking was performed with 3% Bovine Serum Albumin (BSA, Sigma-Aldrich), 0.1% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... were resuspended in 25 µL of 3 M HCl in methanol (90964, Sigma), and incubated at 20 °C for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... in which 3 mL of 1.3 mg/mL cold collagenase P (Sigma 11213865001) and 0.01 mg/mL DNAse (Sigma D5025 ...
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bioRxiv - Bioengineering 2021Quote: ... 3) the shielding oil consisted of 0.5% (v/v) Span-80 (Sigma-Aldrich) surfactant dispersed in mineral oil (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed samples were incubated with blocking solution composed of 3% BSA (Sigma-Aldrich) in PBS for 1 h at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... TF induction was performed by adding 3 μg/mL of Doxycycline (Sigma, D9891) on day 2 ...