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Citations for Millipore Sigma :
3601 - 3650 of 4532 citations for Human LACC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... MDCK cells were transfected with linearized plasmid DNA and selected with either 500 μg/mL G418 (Sigma-Aldrich) or 400 μg/mL Zeocin (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... TRCN0000028509 (sh-AC2) and the scramble control pLKO.1-Puro plasmid (sh-scr) were obtained from Sigma-Aldrich. The sequence of shRNAs are:
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bioRxiv - Neuroscience 2020Quote: ... CGNs (3 × 106 cells) and plasmids were suspended in 70 μl of Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) and exposed to decay pulses using a CUY21-edit II pulse generator (BEX ...
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bioRxiv - Developmental Biology 2019Quote: ... HEK293 cell viability after iOn plasmids transfection was assessed by dye exclusion with Trypan blue solution (0.4%, Sigma). FP expression was either assayed by flow cytometry ...
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bioRxiv - Cell Biology 2019Quote: ... cells that had integrated plasmid were selected in media supplemented with G418 (400 µg mL-1 Sigma-Aldrich) for 10 days ...
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bioRxiv - Microbiology 2020Quote: ... GBS bearing the pFfluc-Erm plasmid must be exposed to the cofactor D-luciferin (Millipore Sigma cat. # L9504), which was prepared in a filter-sterilized stock solution at a concentration of 28 mg/mL in water ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids encoding CaMKII variants were co-transformed with lambda phosphatase into Rosetta 2(DE3)pLysS competent cells (Millipore). Expression was induced overnight with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2020Quote: ... and cargo in a 1:4:1 ratio with PEI reagent (1:3 plasmid to PEI ratio, Sigma). Three days after transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... under a stereoscope and using a glass microcapillary 1-2μl of plasmid DNAs was injected together with Fast Green (0.1%, Sigma) into different ventricles of the organoids ...
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bioRxiv - Biochemistry 2019Quote: ... the corresponding genes were amplified by PCR from S.cerevisiae genome and cloned into the plasmid pETDute-1 (Novagen). To construct N-terminal 6×His tagged Ups1-Mdm35 fusion protein ...
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bioRxiv - Cancer Biology 2020Quote: The puromycin selection gene was replaced with green fluorescent protein (eGFP) cDNA in Lentiviral pLKO.1 plasmid (Sigma). shRNA oligos (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... parasites carrying the WR99210-selected episomal plasmid were cultivated in the presence of 400 µg/ml G418 (Sigma) to select for integrants with the desired modification of the genomic locus ...
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bioRxiv - Molecular Biology 2021Quote: ... Taq pol and StuI genes were subcloned for expression in the pET24-d plasmid (Novagen, Merck Millipore, Germany) using its NcoI and XhoI restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... Taq pol and StuI genes were subcloned for expression in the pET24-d plasmid (Novagen, Merck Millipore, Germany) using its NcoI and XhoI restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... see Table 1 for complete plasmid list) was induced by treating cells with 2 µg/mL doxycycline (Sigma) for at least 72 hours ...
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bioRxiv - Cell Biology 2021Quote: ... transformed with pET:THMT:PTBP3 (empty plasmid from Peti and Page, 2007) at 15°C overnight with 400 μM IPTG (Sigma). Induced pellets were resuspended in low imidazole purification buffer [20 mM sodium phosphate (pH 7.4) ...
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bioRxiv - Cancer Biology 2022Quote: Plasmids for knockdown of CCL2 (TRCN0000301701 and TRCN0000301702) and CCL7 (TRCN0000317599, TRCN0000068135, and TRCN0000068136) were obtained from Sigma. shRNA control plasmid (SHC002 ...
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bioRxiv - Microbiology 2022Quote: Plasmids containing WT or mutant NS1 sequence were transfected into FreeStyle 293F cells using polyethylenimine (PEI) (40K) (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The CAG-tdtomato or CAG-tdtomato-T2A-RHEBCA plasmid (3.5μg/μl) was mixed with the dye Fast Green (0.3 mg/ml,Sigma-Aldrich) and injected (~1 μl/embryo ...
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bioRxiv - Molecular Biology 2024Quote: The stable cas9 expressing Hep3B (Hep3Bcas9) cell line was generated via transfection with Cas9 plasmid (Sigma catalog #CAS9HYGROVP) at a ratio of 6:1 using lipofectamine (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... incubating 500 ng of supercoiled plasmid DNA (pcDNA5 FRT/TO and pOG44) with 100 µM Mirin (Sigma-Aldrich), 3.4 µM doxorubicin (known to exhibit strong DNA intercalation ...
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bioRxiv - Cell Biology 2023Quote: ... All constructs were prepared for transfection using either the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (Sigma-Aldrich) or the NucleoBond Xtra Midi EF kit (MACHEREY-NAGEL) ...
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bioRxiv - Cell Biology 2023Quote: ... All constructs were prepared for transfection using either the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (Sigma-Aldrich) or NucleoBond Xtra Midi EF kit (MACHEREY-NAGEL).
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bioRxiv - Microbiology 2023Quote: ... the plasmid pBSU101- GFP was maintained with the addition of 120 μg/ml of the antibiotic Spectinomycin (Sigma) in the plates as well as growth media.
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bioRxiv - Microbiology 2023Quote: ... Strains with the PCdrA∷gfp and pMH487 plasmids were grown with 60 μg/mL Gentamycin (Sigma-Aldrich, G1914) for plasmid selection ...
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bioRxiv - Microbiology 2023Quote: ... All pCC2Fos replicons were extracted using the GenEluteTM HP Plasmid Miniprep Kit (Sigma Aldrich, St. Louis, Missouri, USA) and NucleoBond® Xtra Midi Plus (MACHEREY-NAGEL ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells growing at ∼70-80% confluency were transfected with 1 μg of the desired expression plasmid (Sigma Aldrich) along with 2nd generation lentiviral packaging and envelope plasmids ...
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bioRxiv - Microbiology 2023Quote: ... We used the PR8 pDZ-HA plasmid as a template and Kod Hot start DNA polymerase (Millipore Sigma). We followed the manufacturer’s recommendations to PCR mutagenize the pDZ-HA open reading frame ...
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bioRxiv - Microbiology 2023Quote: ... We used the pH28D14 HC and LC plasmids as templates and Kod Hot start DNA polymerase (Millipore Sigma). We followed the manufacturer’s recommendations to PCR mutagenize the VH and VL gene segments ...
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bioRxiv - Cell Biology 2023Quote: ... after 42°C heat shock transformation and extracted using the GenElute HP Plasmid Miniprep Kit (Sigma-Aldrich, Merck). To generate pARL-PfCen1-GFP ...
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bioRxiv - Biochemistry 2023Quote: ... of p150 from rubella virus strain RVi/Brooklyn.NY.USA/98/1B CRS (GenBank JN635282; UniProt G3M8F4) was cloned into the pET24a plasmid (Novagen) with a C-terminal hexahistidine purification (His6 ...
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bioRxiv - Bioengineering 2023Quote: To construct the plasmids harboring the retron arrays in their different architectures the pCDF-DUET-1 vector (Novagen) was used as a backbone ...
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bioRxiv - Biochemistry 2023Quote: His-PP-Pex6 1-204 protein (purified by recombinant expression from plasmid pDC69) was digested with 0.1 or 0.2 μg/μL trypsin protease (Sigma) for 10 minutes at 23 °C in 100 mM Tris pH 8.0 buffer ...
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bioRxiv - Microbiology 2023Quote: ... Transformants containing the plasmid were selected on tryptone soy agar (TSA) containing 10 μg/mL chloramphenicol (Cam, Sigma) and incubated for 24-48 h at 30°C ...
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bioRxiv - Microbiology 2024Quote: ... the full NSm ORF was amplified from the pT7riboBUNM plasmid with KOD Hot Start Master Mix (EMD Millipore) using the primers listed in Table S1 ...
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bioRxiv - Biochemistry 2024Quote: ... All plasmids were checked by Sanger sequencing (Microsynth) prior to cloning into a modified pET28b-SUMO vector (Novagen). The recombinant plasmids include an N-terminal 8xHis-tag followed by a small ubiquitin-like modifier (SUMO ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1143-1300 of human RTEL1 were amplified by PCR then subcloned between the EcoRI and XhoI restriction sites of pET28 (Novagen). RTEL1 proteins were expressed as 6-histidine-fusions in the E ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific) FITC (Sigma, F9512) and Anti-human IgG (Fc specific ...
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bioRxiv - Immunology 2021Quote: ... MAb was reacted with antigen coated on the plates and then treated with serially diluted C1q from human serum (Sigma). C1q binding was detected with horseradish peroxidase-conjugated anti-C1q antibody ...
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bioRxiv - Cell Biology 2019Quote: ... S7077) and used at a final concentration of 10 µM. Human recombinant HGF (Cat. Nr. 1404) was purchased from Sigma and used at a final concentration of 100 ng/ml ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 (human epithelial colorectal adenocarcinoma cell line) cells were purchased from ATCC and cultured in DMEM high glucose media (Sigma). The cells were trypsinized using 0.25% trypsin-EDTA and resuspended in the fresh media ...
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bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: The cDNA residues 1-169 of human Rheb (184 amino acid isoform, GenBank accession number EAW53989) was subcloned into the pET28a vector (Novagen), with an N-terminal 6×His-tag ...
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bioRxiv - Developmental Biology 2021Quote: Newborn pups were injected at P2 via the temporal vein with 0.0025mg/g of DLL4-neutralizing (blocking antibody (YW152F, Genentech (31)) or IgG from human serum (cat #I4506, Sigma Aldrich). The pups were sacrificed 48 h after injection at P4 ...
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bioRxiv - Molecular Biology 2021Quote: ... These wells were washed five times using 1× PBST and followed by addition 50 μl/well of anti-human IgG horseradish peroxidase (HRP) (Sigma) diluted in Stabilzyme Noble (Surmodics) ...
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bioRxiv - Pathology 2022Quote: ... biopsies of human xenograft harvested at the indicated time post infection were digested with 0.4 mg/ml Liberase TL (Sigma Aldrich) in CO2-independent medium for 60 min at 37°C with gentle agitation (100 rpm) ...
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bioRxiv - Cancer Biology 2022Quote: ... Metastases and micro-metastases were assessed using IHC with a human mitochondria antibody (Millipore MAB1273 Anti-Mitochondria clone 113-1).
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bioRxiv - Immunology 2019Quote: The sequence-encoding residues Val77–Leu233 of human TNFα was cloned and fused to the N-terminal 6xHis tag in pET-28a+ vector (Novagen) and transformed into Escherichia coli Rosetta (DE3 ...
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ArchR: An integrative and scalable software package for single-cell chromatin accessibility analysisbioRxiv - Genomics 2020Quote: ... MCF7 cells were ordered from ATCC and cultured in EMEM containing 0.01 mg/ml of human insulin (Millipore-Sigma 91077C). MCF10A cells were ordered from ATCC and cultured in DMEM/F12 containing 5% horse serum (Thermo Fisher 16050130) ...
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bioRxiv - Genomics 2020Quote: Twenty-four well plates were coated with: human or rat tail collagen type I (88 ng per well; Sigma-Aldrich), human collagen type III (88 ng per well ...