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Citations for Millipore Sigma :
3801 - 3850 of 4532 citations for Human LACC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: The C19orf43.WT plasmid is comprised of an N-terminal 3x Flag tag followed by human full length C19orf43 cloned into the p3XFlag-CMV-10 vector (Sigma). C19orf43.R7G was obtained by mutating the arginine at position 7 to glycine and C19orf43.R106G was obtained by mutating the arginine at position 106 to glycine ...
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bioRxiv - Bioengineering 2023Quote: ... encoding the human KRASG12V and KRASY64A mutants (residues 1–169) and human SOS1 (residues 564–1049) were cloned as NdeI and XhoI fragments into pET28b (Novagen). His-tagged KRAS•GDP (abbreviated RAS ...
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bioRxiv - Immunology 2022Quote: ... in mid-logarithmic phase was added at a multiplicity of infection of 2 in RPMI with 10% human serum (Sigma). Bacteria were spun down for 5 min at 800 x g and incubated for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and Mirg-/-littermates injected subcutaneously with Growth factor-reduced Matrigel BD) with 400 ng mL-1 recombinant human bFGF (Millipore). After 7 days Matrigel plugs ...
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bioRxiv - Cancer Biology 2023Quote: The mouse E0771 (derived from a spontaneous triple negative mammary tumor that arose in a C57BL/6 mouse) and the human K562 (originally generated from a female leukemia patient) cell lines were cultured in RPMI-1640 (Sigma) supplemented with 10% FCS (GIBCO) ...
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bioRxiv - Developmental Biology 2023Quote: ... (gift from the laboratory of Jeremy Nathans) rabbit anti-LW/MW-opsins (1:200 for organoids, 1:500 for human retinas) (AB5405 Millipore), and mouse anti-Rhodopsin (1:500 ...
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bioRxiv - Immunology 2023Quote: ... in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin, Sigma-Aldrich) for 40 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... followed by streptavidin-Cyanine-3 (1:500; Amersham Bioscience) or by goat anti-human IgG coupled to FITC (1:100, Sigma), as appropriate ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Immunofluorescence in human cells was conducted as previously described using antibodies against tubulin (1:1000 anti-beta tubulin, mouse, T7816, Sigma OR 1:2000/1:1000 anti-alpha tubulin ...
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bioRxiv - Cancer Biology 2023Quote: The U251-MG (U251) human glioblastoma cell line was obtained from ATCC and cultured in low- glucose DMEM (Sigma, D5921) with the addition of 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed and incubated with 100 μl per well of samples prepared as described above and with mouse anti-human αvβ6 (Millipore, MAB2077Z) from 312.5 ng/ml to 1.22 ng/ml to produce the standard curve for 60 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The wells were washed with PBS-0.1% Tween 20 and then incubated with 100 μl of 100 μg/well of biotinylated rabbit anti-human C1q (Sigma) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... GFP measurements in human cell lines were taken on day 4 post-infection and every four days with Guava Easycyte HT instrument (Millipore). The fold change in GFP+ population (normalized to day 4 ...
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bioRxiv - Microbiology 2023Quote: ... a pellet of neutrophils was resuspended in 800 μL HBSS with calcium and magnesium and 200 μL human serum (Sigma). Each 10 mL lithium-heparin coated tube of blood drawn yielded 1 mL of neutrophil-only solution ...
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bioRxiv - Neuroscience 2023Quote: ... The antibodies used in this study were: polyclonal rabbit anti-human HINT1 antibody (1:1000, Sigma, San Luis, MO, USA), and to demonstrate equal loading mouse monoclonal anti-β-actin antibody (1:5000 ...
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bioRxiv - Bioengineering 2023Quote: ... substrates containing 400 nm diameter pores at a density of 2·106 pores·cm-2 were coated with 100 µL of 1 µg/mL human plasma fibronectin (Sigma-Aldrich) in PBS (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... 1:200 dilution of human C-reactive protein (MPBIO) including additional 5 mM CaCl2 or 1:1000 anti-HRP from rabbit (Sigma) in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... and RANTES were simultaneously quantified from the plasma collected from the BM of patients enrolled in the clinical trials using the MILLIPLEX Human Cytokine/Chemokine/Growth Factor Panel A (HCYTA-60K-PX38, Millipore) according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2023Quote: ... were isolated from PBMCs using anti-CD14-antibody-coated magnetic beads (Miltenyi) and cultured in RPMI1640 supplemented with 10% human AB serum (Sigma), pen/strep ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Biochemistry 2024Quote: ... The protein expression of the mouse or human 5-HT3A was induced by the addition of 4 μg/mL tetracycline (Sigma). Valproic acid (Sigma ...
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bioRxiv - Physiology 2024Quote: ... Serum samples were stored at −80 °C until assessed by ELISA according to manufacturer’s instructions (Ultrasensitive Human Growth Hormone ELISA, 22-HGHHUU-E01, ALPCO; mGH, EZRMGH-45K and Rat/Mouse Insulin ELISA, EZRMI-13K, Millipore).
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bioRxiv - Immunology 2024Quote: ... Iron free media was supplemented with set concentrations of human holotransferrin (R&D systems, 2914-HT-001G/Sigma Aldrich, T0665) ranging from 0.0002-0.625 mg/mL ...
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bioRxiv - Immunology 2024Quote: ... An equal volume of IgM antibody at a concentration of 150-200µg/mL was mixed with equal volume of human IgG obtained from blood (Sigma-Aldrich) at a concentration of 1mg/mL (ratio ∼1:5 ...
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bioRxiv - Immunology 2024Quote: ... Human PBMC were pre-activated with 30 ng/ml anti-CD3 (OKT3, Miltenyi) and 50 IU/ml IL-2 (Sigma) and subsequently transduced two times with viral supernatant in the presence of 6 ug/ml polybrene (Sigma ...
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bioRxiv - Physiology 2024Quote: ... Protein lysates were prepared from human RV tissue using modified RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Sigma). Proteins were diluted in 4x Laemmli sample buffer ...
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bioRxiv - Physiology 2024Quote: Immunoblot analysis was carried out on cell or adipose lysates using mouse or human primary antibodies against MPC1 (Sigma #HPA045119), MPC2 (Cell Signaling Technologies #46141) ...
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bioRxiv - Cell Biology 2024Quote: ... the chips were incubated with 0.1% (m/v) gelatin combined with 20 µg/ml human plasma fibronectin (Sigma-Aldrich, 341635) in PBS at 37°C for 1 hour ...
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bioRxiv - Immunology 2024Quote: Single cell solution was obtained from human normal donor and IPF lungs after enzymatic digestion with 10X Liberase (Sigma, 5401127001) and DNaseI (9003-98-9 ...
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bioRxiv - Neuroscience 2024Quote: ... Levels of individual cytokines were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) and involving the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
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bioRxiv - Cell Biology 2024Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
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bioRxiv - Cancer Biology 2024Quote: ... CWR22 (primary prostate tumour) human prostate cancer cell lines were obtained from ATCC and grown in RPMI-1640 (Sigma Aldrich) and supplemented with 1% L-Glutamine (Gibco ...
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bioRxiv - Neuroscience 2024Quote: Binding of cGAS and cytoplasmic mtDNA in human and mouse brain sections was determined by a PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Total protein was extracted from 20-30 mg human frontal cortex using RIPA buffer (PierceTM RIPA Buffer, ThermoScientific, 89901) supplemented with 0.05% CHAPS (Sigma, C3023), 1X complete protease inhibitor (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and perfusate levels of 71 analytes using two separate panels as per the manufacturer’s instructions for use: (1) the Human Cytokine 48-Plex Discovery Assay® (HD48; Millipore MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel Immunology Multiplex Assay ...
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bioRxiv - Neuroscience 2024Quote: ... in primary neuronal lysate after Aβ or gAβ treatment from mouse or human brain were quantified by the Glycerol-3-Phosphate Assay Kit (Sigma), High Sensitivity DHAP Fluorometric Assay Kit (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: The cortex tissue samples from mice or human were lysed in PBS along with PDE inhibitor (IBMX) and PIC (SIGMA) with homogenizer followed by centrifugation at 12500 rpm for 20min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... under air-liquid interface conditions in Greens+ keratinocyte proliferation media (Greens media supplemented with 10 ng/mL human EGF (Sigma)) ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were pulsed overnight at 37°C with 100 μM of individual peptides in serum-free medium (TexMacs) supplemented with 100 ng/µL of human β2-microglobulin (Sigma), followed by the indicated chase kinetics performed in the presence of Brefeldin A (Sigma ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown to 70-80% confluency on surfaces were coated with 10μg/ml of purified human fibronectin (SIGMA-Aldrich). Orbital shear stress was applied to the cells for 18-24 h at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: Human fecal samples (10-20 mg) were resuspended in supplemented Brain Heart Infusion (BHI, Sigma-Aldrich, St. Quentin Fallavier, France) as described in(Motta et al. ...
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bioRxiv - Microbiology 2024Quote: ... albumin from chicken egg white (44 kDa) and γ-globulins from human blood (160 kDa) were purchased from Sigma-Aldrich.
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bioRxiv - Immunology 2024Quote: BMMCs were sensitized for Ag stimulation with dinitrophenol (DNP) coupled to human serum albumin (HSA) (DNP-HSA, Sigma-Aldrich, #A6661) by pre-loading the FcεRI with 0.15 µg/ml IgE (clone SPE-7 ...
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bioRxiv - Biophysics 2021Quote: ... The plasmids were incorporated into pcDNA3.1(+) vectors and were transiently transfected into HEK 293T cells using PEI (Milipore Sigma) as previously described 66 ...
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bioRxiv - Microbiology 2021Quote: ... and oriT from pUC18T-mini-Tn7T-Tp-dsRedExpress were PCR amplified and introduced into a linearized pYES1L plasmid (Novagen) using the Gibson assembly method 46 (Supplementary Fig ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The fluorescent proteins encoded on the plasmid also carries a gene for antibiotic resistance (Gentamicin 418, Sigma-Aldrich: G418). Cells were cultured in autoclaved HL5 medium (per L ...
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bioRxiv - Molecular Biology 2020Quote: ... U2OS cells were first transfected with the pCMV-Tet3G plasmid and selected using 250 µg/ml G418 (Sigma-Aldrich). The G418-resistant clone was maintained as the Tet3G-expressing U2OS cell line ...
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bioRxiv - Microbiology 2019Quote: ... a source of T7 RNA Polymerase was provided by co-transforming another plasmid pAR1219 (Sigma-Aldrich, St. Louis, MO). The plasmids pACD4K-C::emtA and pAR1219 were maintained by selection on chloramphenicol and ampicillin ...
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bioRxiv - Microbiology 2019Quote: ... All media was supplemented with 50μg/ml kanamycin to select for plasmid maintenance and 0.1% of Tween-20 (Polysorbate-20, Sigma-Aldrich) to reduce sticking of cells to the sides of microfluidic devices ...