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Citations for Millipore Sigma :
3251 - 3300 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... After a 2 h blocking step in blocking buffer (PBX with 5% normal goat serum, Sigma-Aldrich, G9023) at RT ...
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bioRxiv - Immunology 2022Quote: ... in PBS 30 minutes at RT and blocked with 2% BSA and 5% Donkey Serum (Sigma-Aldrich, D9663) in PBS during 1 h at RT ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl2) for 2 min and then the DNA digested with 20U DNaseI (cat #: D4527, Sigma-Aldrich) in 1 mL of digestion buffer for 40 sec at RT ...
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bioRxiv - Genetics 2022Quote: ... Experiments for Figure 5 were as Figure 2 with the following changes: 10 μg/mL tunicamycin (InSolution, Sigma) was used ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed using 5% SDS supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich). The following antibodies were purchased from Cell Signaling Technology (Danvers) ...
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bioRxiv - Plant Biology 2021Quote: ... The full length coding sequence of AdACS1/2/3 and AdACO3/5 were inserted into pET-32a (Novagen) vector and then transferred into Escherichia coli strain BL21 (DE3) ...
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bioRxiv - Immunology 2020Quote: ... Primary antibody in 250 μl of cold antibody buffer (20 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 2 mM EDTA, 0.1% digitonin, and protease inhibitor cocktails from Sigma) was added to the tubes and rotated at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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Nanoscale molecular architecture controls calcium diffusion and ER replenishment in dendritic spinesbioRxiv - Neuroscience 2021Quote: ... and in addition of 5′fluoro-2-deoxyuridine + uridine (FUDR) (Sigma; 20 μg and 50 μg/ml, respectively), to block glial proliferation ...
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bioRxiv - Physiology 2022Quote: ... L4 staged worms were picked onto NGM plates containing 49.5 μM 5-fluoro-2’-deoxyuridine (FUDR, Sigma F0503), 50 μg/ml carbenicillin ...
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bioRxiv - Cancer Biology 2019Quote: ... Treatment was followed by pulse-labelling for 20 minutes with 25 μM 5-Chloro-2′-deoxyuridine (CldU, Sigma) and then 20 minutes with 250 μM 5-Iodo-2′-deoxyuridine (IdU ...
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bioRxiv - Cell Biology 2020Quote: ... and Click-iT® EdU (5-ethynyl-2’deoxyuridine) Assay (BCK-EDU488, baseclick GmbH, Merck/Sigma-Aldrich, UK), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells in Accutase were added 1:2 to mTeSR™1 plus 5 μM Blebbistatin (Bleb, B0560, Sigma), pelleted at 150g for 5 minutes ...
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bioRxiv - Genetics 2019Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma-Aldrich, St. Louis, MO, product C4706) and alkylated with 55 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Neuroscience 2022Quote: ... we delivered the Iκ-kinase inhibitor [5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide (TPCA-1; Sigma-Aldrich CAS 507475-17-4 ...
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bioRxiv - Microbiology 2022Quote: ... zebrafish embryos were treated with 180 µM of 1-phenyl 2-thiourea (PTU) (Sigma-Aldrich, 103-65-5), to prevent pigmentation [25] ...
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bioRxiv - Cancer Biology 2024Quote: ... β-estradiol was removed at day 5 from Type 2 medium and DMSO (Sigma, #D2650; final concentration = 0.1%) or 4-hydroxytamoxifen (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μL MTT (5 mg/ml tetrazolium salt 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Sigma-Aldrich) was added to each well and kept in a dark for 4 hours at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were carried out using the indicated antibodies: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), BLM (Bethyl ...
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bioRxiv - Cancer Biology 2023Quote: ... asynchronously growing subconfluent KB2P1.21 or KB2P3.4 cells were labeled with 30μM thymidine analogue 5-chloro-2’-deoxyuridine (CIdU) (#C6891, Sigma-Aldrich) for 20’ ...
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bioRxiv - Neuroscience 2023Quote: ... the larvae were anaesthetized with 0.02% Tricaine for 5-10 minutes and immobilized in 1.5-2% low-melting point agarose (Sigma) in fish water ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Cell Biology 2022Quote: ... reactions were incubated for 2 h on ice before quenching with 5 mM L-Cysteine (Millipore-Sigma, USA). The reactions were then adjusted to 1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... reactions were incubated for 2 h on ice before quenching with 5 mM L-Cysteine (Millipore-Sigma, USA). The reactions were then adjusted to 1% (w/v ...
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bioRxiv - Immunology 2023Quote: ... 2% FCS and 50 μg/ml Lipopolysaccharide neutralizing agent Polymyxin B sulfate (cat # 1405-20-5; Sigma-Aldrich) for 30 min at 37°C while shaking by 750 rpm on a heating block ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells in Accutase were added 1:2 to mTeSR™1 with 5 µM Blebbistatin (Bleb, B0560, Sigma), and pelleted at 150 g for 5 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... Immunofluorescence studies were performed on 5 µm sections fixed in 2% paraformaldehyde (PFA; Millipore Sigma, St. Louisa, MO) for 5 minutes at room temperature (RT).
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bioRxiv - Microbiology 2023Quote: ... VirB or its mutant variants were incubated with 5 mM Tris(2-carboxy-ethyl) phosphine (TCEP; Sigma, USA) for 1 h at room temperature to fully reduce all cysteine residues ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody65 (kind gift of G ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in PBS + 5% NDS and in the second staining (2) 2B4 (1:200, Sigma Eldrich, Cat. # MAB5492), GFAP (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Stock solutions of the NMDA receptor antagonist DL-2-amino-5-phosphonovaleric acid (DL-APV; #5282; Millipore Sigma), GABAA receptor antagonist bicuculline methiodide (#14343 ...
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bioRxiv - Cell Biology 2024Quote: ... All media used for compound dilutions contained 20 mM glucose and 2% DMSO (Sigma Cat. #D2650-5×5ml).
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bioRxiv - Microbiology 2024Quote: ... After 24 h media/inhibitor was aspirated and replaced with 20 μl of 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) and incubated at 37°C in 5% CO2 for 3 h ...
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bioRxiv - Cancer Biology 2024Quote: ... The treatment group received 2 mg/ml Dox (BelaDox) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich), to induce an in vivo knockdown ...
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bioRxiv - Immunology 2024Quote: One million of purified human CD4+ Tsen was activated with an anti-CD3 antibody coated-plate (0.5μg/ml) plus rhIL-2 (5 ng/ml; 11 011 456 001; Sigma). Forty-eight hours post-activation ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.5 and reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich Cat No: C4706) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Gene edition was induced in mESC lines by 5-day treatment with 2 μg/ml Doxycycline (Sigma, D9891). Cell lines expressing the sgRNA that yielded the best percentage of editing were used for further experiments ...
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bioRxiv - Developmental Biology 2020Quote: ... and 4×10-4 M monothioglycerol (Sigma)) with BMP4 (1 ng/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... 4-nitroquinoline 1-oxide (4-NQO) (Sigma). Treatment conditions are indicated in the corresponding figures ...
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bioRxiv - Genomics 2020Quote: ... and 4 × 10−4 M monothioglycerol (Sigma). Media and cytokines were changed daily as follows (Gadue et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Aminopyridine (4-AP, Sigma, Cat# A78403), DL-AP5 (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 4-hydroxytamoxifen (4-OHT) (Sigma-Aldrich; #H6278) was added to the retinal explant media after 7 DIV at 5 mM final concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-Hydroxytamoxifen (4-OHT, Sigma-Aldrich, USA). Further information on cell lines and clones in Supplementary Data.
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bioRxiv - Neuroscience 2021Quote: 4-aminopyridine (4-AP; Sigma Aldrich, USA) was used to block IKdr ...
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bioRxiv - Neuroscience 2019Quote: ... 200µM 4-aminopyridine (4-AP, Sigma Aldrich), 50µM 6-Cyano-7-nitroquinoxaline-2,3-dione disodium salt (CNQX ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Hydroxytamoxifen (4-OHT) (H6278, Sigma-Aldrich) was handled as previously described37 ...
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bioRxiv - Developmental Biology 2020Quote: ... and 4-hydroxytamoxifen (4-OHT, Sigma H6278) were mixed with 100% ethanol until completely dissolved ...
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bioRxiv - Developmental Biology 2021Quote: ... and 4×10-4 M monothioglycerol (Sigma)) with BMP4 (1 ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... 1 µM 4-hydroxytamoxifen (4-OHT; Sigma) for 1h at 37°C prior to infection and during the course of infection ...