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Citations for Millipore Sigma :
3351 - 3400 of 4532 citations for Human LACC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and human embryonic kidney (HEK) 293T or 293 cells were cultured in Dulbecco’s modified essential medium (DMEM) (Sigma) with 10% foetal calf serum (FCS ...
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bioRxiv - Pathology 2023Quote: Sub confluent human RPE cells were treated with 500 μM H2O2 (Sigma-Aldrich #H1009, Saint Louis, MO, USA) after sci control or siRNA transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were cultured in ImmunoCult-XV T cell expansion medium supplemented with human serum (10%; Sigma, USA), Penicillin-Streptomycin and IL-2 (100 IU/ml ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Microbiology 2023Quote: ... Carbohydrate sources for the human fecal matter inoculated cultures were apple pectin (2.5 g/L, Sigma-Aldrich, USA) and porcine mucin (2.5 g/L ...
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bioRxiv - Bioengineering 2023Quote: ... This suspension was incubated with an equal volume of 71.5 μg/mL AF647 anti-human VEGF antibody (Sigma) for 30 minutes at 37°C with gentle vortexing every 10 minutes before washing excess antibody with one high-dilution wash (>100x nanovial volume) ...
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bioRxiv - Cell Biology 2022Quote: esiRNAs specific to human caveolin-1 or ARHGAP29 and a non-targeting esiRNAs were purchased from Sigma-Aldrich and transfected at a final concentration of 50 nM using Dharmafect (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Petrisoft™ easy coat hydrogel 0.2 kPa or 50 kPa 6-well plates were coated with 5μg/ml human fibronectin (Sigma) and RNA lysates harvested as described.
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bioRxiv - Genomics 2023Quote: Luminex assays were performed using the Milliplex Human Cytokine/Chemokine Magnetic Bead Panel (HCYTOMAG-60K-41, Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... spermatozoa from JF1/MsJ males were capacitated in Human Tubal Fluid medium (Merck Millipore, Cat#MR-070-D) supplemented with 10 mg ml−1 Albumin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: 2 μg GST-tagged NuSAP point mutants were incubated with 50 ng human recombinant Aurora A (Sigma-Aldrich) in a water bath at 30°C for 30 min in kinase buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... The cleavage fragments were analyzed by western blot with either mouse anti-human fibrinogen polyclonal antibody (Sigma-Aldrich) at a 1:5,000 dilution ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with 10 µg/ml of capture anti-human IgG (Sigma Aldrich, I2136) in PBS ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, cat. no. D6429) supplemented with 10% heat-inactivated foetal bovine serum (hiFBS ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were expanded in the presence of 10 U/mL recombinant human IL-2 (11147528001, Sigma). Human CD8+ T cells were purified from donor PBMCs (NHSBT or Karolinska Hospital ...
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bioRxiv - Immunology 2022Quote: ... HRP-conjugated anti-mouse IgG Fab (Cat# A9917) and anti-human IgG Fab (Cat# SAB4200791) were from Sigma. HRP-conjugated anti-mouse IgG (Cat# PA1-28568 ...
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bioRxiv - Neuroscience 2023Quote: ... 50mg fresh frozen postmortem human brain tissue was homogenized in Nuclei EZ Lysis buffer (NUC101-1KT, Sigma-Aldrich) and incubated for 5 min before being passed through a 70µm filter ...
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bioRxiv - Bioengineering 2023Quote: ... we pre-treated 48-well plastic plates with 200 μL of 20 μg/mL human fibronectin (Milipore Sigma) for 10 minutes at 37 °C under 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: E0.5 embryos were cultured in embryo culture medium (EmbryoMax® Human Tubal Fluid, Sigma-Aldrich, # MR-070-D) under mineral oil for embryo culture (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... HUDEP-2 were maintained in Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 330 μg/mL human holo-transferrin (Sigma), 10 μg/mL recombinant human insulin (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... Cells were seeded at 8-8.5×106 cells in 75 cm² or 16.5×106 cells in 150 cm² culture flasks (TPP) coated with 0.25% fibronectin from human plasma (Sigma) and 1% extracellular matrix from Engelbreth-Holm-Swarm murine sarcoma (Sigma ...
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bioRxiv - Immunology 2023Quote: Human IAV strains were propagated in MDCK cells in the presence of 1 µg/ ml TPCK trypsin (Sigma) and harvested after 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured using Fibronectin/Collagen/BSA ECM mixture-coated petri dishes (10µg/mL Human Fibronectin, Sigma Cat. No. F2006; 100µg/mL BSA, Sigma Cat.No.126575 ...
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bioRxiv - Cell Biology 2024Quote: ... was purchased from ATCC (TIB-202) and LX-2 human stellate cells were purchased from Sigma Aldrich (SCC064). LSECs were cultured in endothelial cell basal medium-2 (EBM-2 ...
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bioRxiv - Biophysics 2024Quote: ... For microfluidic surface endothelialization the channels were incubated overnight at 4°C with 10% human fibronectin (Sigma-Aldrich). HUVECs (Lonza ...
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bioRxiv - Microbiology 2024Quote: ... Baby Hamster Kidney (BHK) and Human Embryonic Kidney (HEK) 293 were grown in Dulbecco’s modified eagle’s medium (DMEM; Sigma) containing 10% foetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... cells were plated in 48-well plates pre-treated with 50 mg/mL human fibronectin (FC010-10MG, Millipore) and incubated for 24 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... To this purpose culture dishes/glass slides were first coated with fibronectin (Sigma-Aldrich, Darmstadt, Germany, human plasma) at 5 µg/cm2 concentration diluted in sterile PBS ...
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bioRxiv - Physiology 2024Quote: ... were incubated in hypoxia condition in the hypoxia chamber with TGFβ1 (recombinant human TGFβ1: 15ng/ml, Cat #T7039, Sigma)25 for 72 h ...
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bioRxiv - Physiology 2024Quote: ... were incubated in hypoxia condition in the hypoxia chamber with TGFβ1 (recombinant human TGFβ1: 15ng/ml, Cat # T7039, Sigma)10 for 72 h ...
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bioRxiv - Immunology 2024Quote: ... purified human Tsen were pre-treated with or without the proteasomal inhibitor MG132 (10 µM; M7449; Sigma-Aldrich) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... we used commercially available ELISA kits: hs-CRP Human C-Reactive Protein ELISA Kit (#RAB0096, Sigma-Aldrich, Germany) and Mercodia Insulin ELISA (#10-1113-01 ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% penicillin/streptomycin supplemented with 10 ng/ml EGF (Epidermal growth factor human, Sigma-Aldrich, Cat#E9644) and FGF (Fibroblast growth factor basic human ...
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bioRxiv - Cell Biology 2024Quote: ... and human embryonic kidney (HEK) 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (D6546; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: ... in addition to 100 μg/mL ampicillin for plasmid maintenance and 20 ng/mL anhydrotetracycline (AHT, Sigma) for induction of Cfr expression ...
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bioRxiv - Microbiology 2021Quote: ... parasites containing the episomal plasmids selected with WR99210 were grown with 400 µg/ml Neomycin/G418 (Sigma) to select for integrants carrying the desired genomic modification as described previously (Birnbaum et al. ...
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bioRxiv - Microbiology 2019Quote: ... the plasmid containing the insert was linearized and purified with Gene-Elute PCR Clean-Up Kit (Sigma). DNA concentration was measured with Qubit (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: A mix of endotoxin-free plasmid EB3-EGFP (1.5mg/mL) and vGlut-mcherry (1.2mg/mL) and 0.5% Fast Green (Sigma) was injected into one lateral hemisphere of E15.5 embryos using a Picospritzer III (Parker) ...
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bioRxiv - Molecular Biology 2020Quote: ... coli EF-G (704 aa, C-terminally His6-tagged) cloned into pET24a+ plasmid (Novagen, kanamycin resistance vector) was transformed into an E ...
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bioRxiv - Microbiology 2021Quote: ... The mCherry plasmid was created by cloning mCherry into the multiple cloning site of pTriEx-3 (Novagen) using the Gibson Assembly Cloning Kit (New England BioLabs) ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection of plasmids in MEF and HEK293T was carried out using GeneJuice Transfection Reagent (Merck Millipore, 70967) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with 1–2 μg of pcDNA5 FRT/TO plasmids using GeneJuice Transfection Reagent (Millipore) on to 1×105 adhered U–2–OS or U–2–OS Flp-In T-Rex cells in 2 mL media in a 3.5 cm glass bottom dish (FD35–100 ...
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bioRxiv - Biochemistry 2022Quote: Cells expressing plasmids of interest were grown in Mueller-Hinton broth (Sigma, 100µg/mL ampicillin, pH 7.0) from a single colony to an OD of 0.2 at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Six micrograms of lentiviral plasmid and 2 μg of each helper plasmid were suspended in 0.6 mL Dulbecco’s Modified Eagle’s Medium (SIGMA) containing 36 μg of PEI Max for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Table S3) and subcloned it into the NdeI/XhoI sites of a pET-22b(+) plasmid (Merck Millipore) so that its expression is driven by an IPTG-inducible T7 promoter ...
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bioRxiv - Biochemistry 2021Quote: ... The media containing the plasmids were filtered with a 0.22µm Steriflip Disposable Vaccum Filter Unit (Millipore Sigma) and gently mixed with PEI ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were digested with NdeI and BamHI and inserted into the pET-15b plasmid (Novagen) digested with the same enzymes ...
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bioRxiv - Microbiology 2019Quote: ... pIEX-10:Neo plasmid carries the neomycin resistance cassette and provides resistance to the antibiotic G418 (Sigma) which allows for selection of transfected cells ...
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bioRxiv - Cell Biology 2019Quote: ... All constructs were prepared for transfection using the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (Sigma-Aldrich).