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Citations for Millipore Sigma :
3201 - 3250 of 4532 citations for Human LACC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... These primary monocytes were cultured in RPMI 1640 media supplemented with 10% human serum (H4522, Sigma Aldrich).
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bioRxiv - Biophysics 2023Quote: ... we used three primary monosaccharides found in the human diet: D-glucose (Sigma-Aldrich, St. Louis, MO), D-fructose (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... we first computed the frequency of each trinucleotide context in the human genome using the function get_trinuc_norm from the R package SigMA (https://github.com/parklab/SigMA). Next ...
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bioRxiv - Immunology 2023Quote: ... After addition of 50 µl of 20 ng dinitrophenyl30-40-conjugated human serum albumin (DNP-HAS; Sigma)/ml in RPMI/DTT ...
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bioRxiv - Molecular Biology 2023Quote: Human and mouse cells and muscles were homogenized on ice in RIPA buffer (R0278-50ml, Sigma-Aldrich) supplemented with protease inhibitors (P8340 ...
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bioRxiv - Systems Biology 2023Quote: The human lung carcinoma cell line A549 was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma, D5796) supplemented with 10% FBS (Nichirei Biosciences ...
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bioRxiv - Genomics 2023Quote: ... medium was replaced by differentiation medium (Skeletal Muscle Basal Medium containing 10 µg/ml human insulin [Sigma #I2643] ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from human and animal PFC samples using TRIzol Reagent (Sigma-Aldrich, Taufkirchen, Germany). To ensure proper handling of the RNA ...
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bioRxiv - Neuroscience 2023Quote: Nuclei were extracted from frozen human brain tissues by using Nuclei EZ Prep (Millipore-Sigma, NUC-101) following the manufacturer’s manual ...
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bioRxiv - Neuroscience 2023Quote: Nuclei were extracted from frozen human brain tissues by using Nuclei EZ Prep (Millipore-Sigma, NUC-101) following the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used in this study included rabbit anti-human monoclonal antibodies of ONECUT2 (Sigma HPA057058) at 1:50 ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with Protein A Agarose/Salmon Sperm DNA and 5 μg human IgG (Sigma, Cat# I4506) or PPAR-γ Antibody (E-8 ...
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bioRxiv - Cancer Biology 2023Quote: ... Albumin was measured in the cell culture medium by Human ALB/Serum albumin ELISA Kit (Sigma-Aldrich) and in the cell lysate by qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... Human DRG neurons were plated and grown on glass coverslips coated with poly-D-lysine (Sigma #P7405). Cultured DRG neurons were plated for 24 hours before being treated ...
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bioRxiv - Cell Biology 2023Quote: ... human HT1080 fibrosarcoma cells (ACC315; DSMZ Braunschweig) were cultured in Dulbecco’s modified Eagle medium (DMEM; Sigma Aldrich), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... or a non-target negative control gRNA (Human Sanger Arrayed Whole Genome Lentiviral CRISPR library, Sigma-Aldrich) using polyethylenimine (PEI ...
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bioRxiv - Physiology 2024Quote: ... followed 48 h later by injection of 5 IU of human chorionic gonadotrophin (hCG) (Sigma Cat.# CG10). Thirteen to fourteen hours after hCG injection ...
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bioRxiv - Evolutionary Biology 2024Quote: PC-9 are human adenocarcinoma cells derived from undifferentiated lung tissue were obtained from Sigma (Sigma, USA). PC-9 cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Human pancreatic cancer cells (BxPC-3; ATCC, CRL-1687) were cultured in RPMI-1640 medium (Sigma‒Aldrich) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2024Quote: The in vitro phosphorylation is conducted using the human Src-GST protein (Sigma-Aldrich, Catalogue number: S1076) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was cleaved from the GST tag by incubating the beads with human thrombin (Sigma-Aldrich) in the presence of 50 µM CaCl2 overnight at 4 °C with rocking ...
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bioRxiv - Microbiology 2024Quote: Human U937 monocytic cells were propagated and differentiated into macrophages using phorbol myristate acetate (PMA, Sigma-Aldrich) as previously described (Tanner et al. ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were stored at –20 °C and insulin secretion was quantified using human (Millipore Cat. # HI-14K) insulin radioimmunoassay kits.
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bioRxiv - Molecular Biology 2024Quote: Human neuroblastoma SH-SY5Y cells (ATCC CRL-2266) were cultured in Dulbecco’s modified Eagle’s Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... pDEST42-Ctdnep1_C-ter plasmid was transformed in Rosetta™ 2(DE3)pLysS Competent bacteria (Merck Millipore), Bacteria cultures (500 ml ...
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bioRxiv - Cell Biology 2019Quote: ... The fragment was cloned between the SalI and NotI restriction sites of the pET23a(+) plasmid (Novagen) resulting in a pEt23_CAP-D2_Ct construct which contains the cassette T7 promoter∷T7 tag-CAP-D2Ct-His tag ...
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bioRxiv - Cell Biology 2019Quote: ... Hela and HAP1 cells were transfected with 1 µg of corresponding plasmid using GeneJuice® (Novagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: HK2 cells were transfected with different plasmids using X-tremeGENE HP DNA transfection reagent (Sigma-Aldrich) according to the manufacturer’s instructions and incubated for 18-24 h before fixation or live-cell imaging ...
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bioRxiv - Synthetic Biology 2021Quote: ... the truncated AtCCD7 gene was amplified by PCR and cloned into the pCDFDuet-1 plasmid (Novagen) using NcoI and NotI to yield the plasmid pCDFDuet-trAtCCD7 ...
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bioRxiv - Genomics 2021Quote: ... HEK293 and NSC were transfected with STARR-seq plasmid containing enhancer regions using polyethylenimine (PEI, Sigma) or Lipofectamine™ Stem Transfection Reagent (Thermo Scientific ...
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bioRxiv - Pathology 2021Quote: ... Purification of plasmids was achieved using the Endotoxin-free Maxi prep kit (Sigma-Aldrich, St.Louis, MO), after which the constructs were injected into the mice.
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bioRxiv - Cell Biology 2022Quote: ... 5’-AGTTTCTCTGTCCGATTTAAA-3’] are in the plasmid backbone pLKO.1-CMV-tGFP and were from Sigma Millipore (St Louis ...
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bioRxiv - Developmental Biology 2022Quote: ... GJA1 siRNA- or plasmid DNA-transfected RPE1 cells were cultured on fibronectin (Sigma Aldrich, MO, USA)-coated cover glasses ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting PCR products were ligated into the same sites in plasmid pET-22b(+) (Novagen). An identical strategy was used to generate plasmids pET-YpTatB22-220 and pET-YpTatB22-91 for expressing and purifying full-length or truncated versions of TatB from Y ...
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bioRxiv - Immunology 2020Quote: ... DNA plasmids were transfected into Lenti-X™ 293T cells using GeneJuice® (Novagen, Waltford, UK) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Plasmids were extracted using the Qiagen Miniprep Kit using 425-600 μm glass beads (Sigma Aldrich) for mechanical lysing of yeast cells ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was isolated from the transformant and transformed into Rosetta (DE3) cells (Merck Millipore, USA). For purification ...
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bioRxiv - Cell Biology 2022Quote: ... HK2 cells were transfected with different plasmids using X- tremeGENE HP DNA transfection reagent (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... cells transfected with the KRASWT plasmids were always supplemented with 15ng/ml of doxycycline (Sigma-Aldrich). Cells were incubated for 24 h at 37°C and harvested ...
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bioRxiv - Molecular Biology 2022Quote: ... and HEK293T cells were transfected with pcDNA3.1/PA-hZnT7ΔHis-loop plasmids using PEI reagent (Sigma-Aldrich) at a ratio of 50 μg DNA to 1.5 ml PEI reagent (1 mg ml-1) ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloning of it into NdeI-XhoI site of expression plasmid pET-22b(+) (Novagen, WI, USA).
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bioRxiv - Neuroscience 2024Quote: ... a mixture of PEI and Plasmid DNA diluted in DPBS (Dulbecco Phosphate Buffered Saline) (Sigma; D8537) was added to the cell culture media to a final concentration of ...
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bioRxiv - Cell Biology 2023Quote: ... p15A-CmR backbone plasmids (see Table S2) were maintained using 30 µg/mL chloramphenicol (Sigma-Aldrich) in plates ...
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bioRxiv - Neuroscience 2024Quote: A mix of endotoxin-free plasmid preparation (2-5 mg/mL) and 0.5% Fast Green (Sigma) mixture was injected using a Picospritzer III (Parker ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng plasmid DNA was combined with 1.5 µL polyethyleneimine (PEI, 1 mg/mL, Sigma, #p3143) in 50 µL Optimem (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Plasmid constructs pLKO.1/shMfrn1 (TRCN0000044010) and pLKO.1/shFTH1 (TRCN0000029432) were purchased from Sigma-Aldrich, and V5-tagged FSP1 (V5-FSP1 ...
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bioRxiv - Bioengineering 2023Quote: ... The linearized plasmid was subsequently ligated to oligo pairs to create long ssDNA overhangs (Sigma-Aldrich) on either end ...
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bioRxiv - Neuroscience 2023Quote: ... and mock plasmids were transfected into HEK293 suspension cells derived from tsA201 (Millipore Sigma, 96121229-1VL) using Polyethylenimine (Polysciences ...
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bioRxiv - Developmental Biology 2023Quote: ... predicted mature peptide regions of ZmRALF1/2/3/5 genes were cloned into plasmid pET32b (Novagen) with gene specific primers as indicated (Supplemental Table S1) ...