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Citations for Millipore Sigma :
3551 - 3600 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... MYC forward 5’-TGAGGAGGAACAACAAGATG-3’ and reverse 5’-ATCCAGACTCTGACCTTTTG-3’ and GAPDH forward 5’-CTTTTGCGTCGCCAG-3’ and reverse 5’-TTGATGGCAACAATATCCA-3’) (Sigma-Aldrich), the Radian SYBR Green Lo-ROX qPCR Kit (Alkali Scientific ...
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bioRxiv - Immunology 2022Quote: ... The sequences of forward and reverse primers for εGLT (fw: 5′-CTGTCCAGGAACCCGACAGA-3′; rev: 5′-TGCA GCAGCGGGTCAAG-3′) and γ1GLT (fw: 5’-CCAGGGCAGGGTCAGCA-3’; rev: 5’-GGTGCTCTTGGAGGAGGGT-3’) (Sigma-Aldrich) and their corresponding FAM-labelled MGB probes (εGLT ...
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bioRxiv - Molecular Biology 2020Quote: ... 2% (v/v) 2-mercaptoethanol and 2 mM PMSF) (Sigma-Aldrich) and homogenize ...
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bioRxiv - Neuroscience 2020Quote: Animals were anesthetized with avertin (2, 2, 2-Tribromoethanol (Sigma-Aldrich), 125-250 mg/kg ...
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bioRxiv - Cell Biology 2022Quote: ... 2-Aminoethyl diphenylborinate (2-APB; Sigma), caffeine (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 2-Deoxyglucose (2-DG, Sigma #D6134) was administered via IP injection at 0.5 mg/g bodyweight ...
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bioRxiv - Cancer Biology 2021Quote: ... SMF cells and EMT6 cells were transduced at 50% confluency in 6-well plates with 1 mL of concentrated lentivirus and 6 µg/mL polybrene (Sigma 107689) at 1000xg and 33°C for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... the electrode surfaces of 6-well Axion plates (Axion Biosystems, CytoView MEA 6) were coated with 10 mg/mL poly-D-lysine (Sigma, P7280) at room temperature overnight ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were resuspended in 6 mL of cold water and lysed by adding them dropwise to 6 mL of boiling 8% SDS (Sigma-Aldrich) within 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured on Greiner Bio-One CELLSTAR Polystyrene 6-well Cell Culture Multiwell Plates coated first with Poly-L-ornithine hydrobromide (6 μg/mL in 1xPBS (Sigma-Aldrich), 1 h at 37℃ ...
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bioRxiv - Neuroscience 2024Quote: ... 500’000 cells per well were seeded as single cells onto new 6-well plates (6 WPs) coated with vitronectin and cultured in mTESR+ medium supplemented with thiazovivin (SML1045, Sigma Aldrich). 2 μL of each viral construct (TetO-Ngn2-P2A-puromycin ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’-GATCCCCAAATCT-3’ and 5’-GATCAGAT[BtndT]TGGG-3’ with 5’ end phosphate (Sigma-Aldrich), were annealed (81 µl of each Oligo 100 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’-GATCCCCAAATCT-3’ and 5’-GATCAGAT[BtndT]TGGG-3’ with 5’ end phosphate (Sigma-Aldrich), were annealed (81 µl of each Oligo 100 µM ...
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bioRxiv - Biochemistry 2020Quote: ... Total extract was clarified by 20 min centrifugation at 30,000 rcf at 4°C and the soluble fraction was loaded on affinity chromatography over 5 mL of NiNTA resin (Sigma-Aldrich Merck, Darmstad Germany). Resin was step-washed with 10 mL of buffer A supplemented with 10 ...
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bioRxiv - Biophysics 2020Quote: ... the molds were sterilized using 70 % ethanol and the wells were coated with a Poloxamere solution in ddH2O over night at 4 °C (5 % Pluronic® F-127, Sigma, St. Louis, USA) to render the surface non-adhesive ...
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bioRxiv - Molecular Biology 2019Quote: The bands were visualized using an acid phosphatase-conjugated secondary antibody and nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP, Sigma-Aldrich, St. Louis, USA) substrate system ...
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bioRxiv - Cell Biology 2020Quote: ... then membranes were blocked overnight at 4°C in 5% milk prepared in 1x TBS – 0.1% Tween20 supplemented with Dibenzocyclooctyne-PEG4-biotin conjugate (Sigma-Aldrich #760749; 50μM final concentration). Membranes were washed three times in 1x TBS – 0.1% Tween20 for 10 min each ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... tracheotomized and their lungs were installation-fixed for 5 minutes at 20cm H2O hydrostatic pressure with 4% (w/v) paraformaldehyde (PFA) (Sigma-Aldrich, Oakville, ON, Canada), pH 7.4 ...
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bioRxiv - Pathology 2021Quote: ... animals were overdosed with pentobarbital sodium and transcardially-perfused with phosphate-buffered saline (PBS) (200 ml) followed by 4% paraformaldehyde (PFA, 200 ml) fixative and then 5% gelatin (20ml in PBS Sigma-Aldrich, G2625, Darmstadt, Germany) solution containing FITC-albumin (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... 5% normal donkey serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (EMD Millipore, cat. Ab3786, 1:300), then at room temperature for 1 hour with Alexa Fluor 594-conjugated secondary antibody (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... fixed in 4% formaldehyde (Thermo 50-980-487; 12 min, 20°C) and then blocked with 5% normal donkey serum (Sigma-Aldrich D9663; 30 min) and Triton X-100 (0.01% in PBS) ...
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bioRxiv - Bioengineering 2024Quote: ... Phosphatase activity was determined by a chromogenic reaction using 5-bromo-4 chloro-3 indolyl phosphate and nitroblue tetrazolium (Sigma, St. Louis, MO, USA) as substrates.
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bioRxiv - Bioengineering 2023Quote: ... Confluent monolayers were treated with 8-(4-chlorophenylthio)adenosine 3′,5′-cyclic monophosphate sodium salt (cPT-cAMP; Sigma-Aldrich, cat# C3912, 25-250 µM) Ro 20-1724 (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... the molds were sterilized using 70 % ethanol and the wells were coated with a Poloxamere solution in ddH2O over night at 4 °C (5 % Pluronic® F-127, Sigma, St. Louis, USA) to render the surface non-adhesive.
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bioRxiv - Immunology 2023Quote: ... or RPMI 1640 + 10% PS as negative non-stimulated (NS) control for 15 h followed by additional 4 h in the presence of 5 μg/mL Brefeldin A (Sigma-Aldrich, cat.: B7651-5MG) and 2 μM monensin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Guts were incubated overnight at 4°C with a primary anti-catalase antibody (C0979, Sigma; 1:250 in 5% w/v BSA in PBS). After three washes with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... the tamoxifen active metabolite 4-hydroxytamoxifen (4-OHT, Sigma, cat#H6278) was used instead of tamoxifen ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently fixed overnight at 4°C in 4% paraformaldehyde (Sigma). Next ...
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bioRxiv - Biophysics 2021Quote: ... 4 cm by 4 cm in chloroform (Sigma Aldrich, analytical standard) and placed the piece of paper inside the plastic container with the recorded fungal substrate.
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bioRxiv - Neuroscience 2020Quote: ... QX314 chloride and 4-aminopyridine (4-AP) were from Sigma-Aldrich, (St ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were induced using 4-Hydroxytamoxifen (4-OHT) (H7904; Sigma H7904) from fresh or pre-heated (65°C for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Shield-1 (Cheminpharma LLC) and 4-hydroxytamoxifen (4-OHT) (Sigma-Aldrich) at 1μM were added for one hour to allow TRF1 to enter the nucleus prior to live imaging or two hours prior to fixing ...
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bioRxiv - Microbiology 2022Quote: ... 3 days post-infection 4 mL of paraformaldehyde 4% (#158127, Sigma) was added and incubated overnight at 4ºC ...
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bioRxiv - Immunology 2023Quote: ... 100 μM 4-aminobenzoic acid hydrazide (4-ABAH, Sigma-Aldrich, A41909) or 20 μM disulfiram (Tocris ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were fixed overnight at 4°C in 4% paraformaldehyde (Sigma) in PBST ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were fixed overnight at 4°C in 4% paraformaldehyde (Sigma) in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... cells were treated with 1 μM 4-hydroxytamoxifen (4-OHT) (Sigma) and 1 μM Shield1 (Clonetech Labs ...
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bioRxiv - Plant Biology 2024Quote: ... through quantification of the fluorescence of 4-MU (4-Methylumbelliferone, Sigma) reaction product ...
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bioRxiv - Molecular Biology 2023Quote: ... 4-Hydroxytamoxifen (4-OHT) and Etoposide were obtained from Sigma Aldrich, BafilomycinA1 was purchased from Cambridge Bioscience and BrDU was purchased from Abcam.
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bioRxiv - Molecular Biology 2023Quote: ... 1 µM or 500 nM 4-Hydroxytamoxifen (4-OHT; Sigma # H7904) for 4 hours to induce Fok1- or AsiSI-dependent DSB ...
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bioRxiv - Cell Biology 2024Quote: ... 4-Hydroxynonenal (4-HNE) was purchased from Millipore Sigma (Burlington, MA). ATP ...
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bioRxiv - Molecular Biology 2024Quote: ... For metabolic labeling with 4-TU (4-thiouracil, Sigma Aldrich, 440736), the cell suspension was transferred to 50 ml Falcons for labeling in a water bath at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
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bioRxiv - Microbiology 2020Quote: ... HB10Y samples were titered prior to freezing by serially diluting samples in 10 millimolar (mM) of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Sigma PN#H4034-100G) + 10% Sucrose (Sigma PN #S7903-250G) ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2 -4 hours at RT with the following antibodies at the following concentrations: rabbit Cone Arrestin (1:3000, Millipore Sigma, cat. #AB15282), chicken B-galactosidase (1:3000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in PBS containing 0.02% Triton X-100 and 2% NDS overnight at 4°C with primary antibodies: anti-ChAT (1:500, goat polyclonal, Millipore Cat# AB144P, RRID:AB_2079751) and anti-phosphorylated ribosomal protein S6 (p-rpS6240/244 ...
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bioRxiv - Plant Biology 2021Quote: ... 2, 4-D (from Plant Media, in 1mM stock with pure ethanol) and NAA (from Sigma-Aldrich, in 10mM stock with pure ethanol) were added to the medium to a final concentration of 0.1 mM ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4, 100 mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA, Roche complete protease inhibitor set, and Sigma phosphatase inhibitor set), incubated on ice for 30 min ...
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bioRxiv - Cancer Biology 2019Quote: ... and finally with the refractive index matching solution BABB-D4 containing 4 parts BABB (benzyl alcohol + benzyl benzoate 1:2, Sigma, 24122 and W213802), 1 part diphenyl ether (DPE ...
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bioRxiv - Immunology 2019Quote: Cells were plated at 1 × 105 cells per well of a 4-well Nun-TekTM Chamber slide™ (1.8 cm2) or of a 24-well plate (2 cm2) (Sigma-Aldrich, Gilligham, UK) with glass coverslip in high glucose DMEM supplemented with 10% FBS and 1% P/S and left at room temperature for 1 hour to adhere evenly across the plate ...