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Citations for Millipore Sigma :
3601 - 3650 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... JQ1 was delivered every other day via intraperitoneal injection at a concentration of 50mg/kg in a 1:4 DMSO:10% (2-Hydroxypropyl)-β-cyclodextrin (Sigma Aldrich; 389145) vehicle starting the day of TAC surgery ...
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bioRxiv - Microbiology 2019Quote: ... without L-glutamine and without phenol red (Biochrom, Berlin, Germany) supplemented with 30 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma-Aldrich, Taufkirchen, Germany), pH 7.4 ...
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bioRxiv - Neuroscience 2019Quote: ... Free-floating slices were washed for 3 × 10 min periods in PBS before blocking and permeabilization for 2 h with 0.3 % Triton X-100 in PBS and 4% normal goat serum (Sigma-Aldrich, Oakville, Ontario, Canada). Slices were incubated with streptavidin conjugated with Alexa Fluor 555 or 546 (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Water was collected within a depth of 0.5 m in 2 L plastic bottles and filtered within 4 h in the lab through 0.22 μm Sterivex® filters (EMD Millipore, Darmstadt, Germany) connected to a peristaltic pump (EMD Millipore ...
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bioRxiv - Microbiology 2022Quote: ... HB10Y samples were titered prior to freezing by serially diluting samples in 10 millimolar (mM) of 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES, Sigma PN#H4034-100G) + 10% Sucrose (Sigma PN #S7903-250G) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with secondary antibodies at 4°C for 2 days in CF™ 633 Anti-Rabbit IgG H+L 1:1000 (Sigma-Aldrich; SAB4600132), Alexa Fluor® 488 AffiniPure Donkey Anti-Goat IgG H+L 1:800 (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2023Quote: ... 3 μl of the reducing reagent (mixture of 0.2 g 1-amino-2-naphtol-4-sulfonic acid (Millipore-Sigma, Burlington, MA, USA, #08751) with 0.2 g sodium bisulfite (Millipore-Sigma ...
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bioRxiv - Immunology 2023Quote: ... Media was removed from the apical compartment of the transwell and replaced by 200µL of fresh medium containing of 2 mg/mL fluorescein isothiocyanate (FITC)-labelled dextran (4 kDa) (Sigma-Aldrich, # 46944-500MG-F). As a negative control and to calculate the background ...
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bioRxiv - Physiology 2023Quote: ... In order to substitute cholesterol in the patch with cholest-4-en-3-one (cholestenone) the bath solution was replaced with 2 mM MBCD solution saturated with cholest-4-en-3-one (Millipore-Sigma, St. Louis, MO).
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bioRxiv - Cell Biology 2023Quote: Cells were arrested in prometaphase by incubating them for 2 – 4 h in 200 ng ml-1 nocodazole (Sigma-Aldrich, 31430-18-9). Mitotic exit was induced by adding flavopiridol (Tocris Bioscience ...
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bioRxiv - Biophysics 2023Quote: Inhibition of Rho-kinase on NK92 cells (Fig. 4) is performed with Y-27632 Rock-inhibitor (Sigma-Aldrich, cat. no. 129830-38-2). Y-27632 Rock-inhibitor is dissolved in DMSO (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... 24.75 µl of activity assay buffer (0.25% w/v taurocholic acid in citrate/phosphate buffer) containing 2 mM 4-methylumbelliferyl-β-D-glucopyranoside substrate (4MU-Gluc; Sigma-Aldrich, # M3633) in activity assay buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... vorticoides at different developmental stages were incubated in 10-5 M serotonin (5-HT) and 5-hydroxytryptophan (5-HTP) (Sigma-Aldrich, USA) for 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’-GACAUAUUUGAUAAACUUAAA-3’, 5’-GUUAUCAGUCUGAGCCAGG-3’), human DRP1 (5’-GCAGAAGAAUGGGGUAAAU-3’, 5’-UCCGUGAUGAGUAUGCUUU-3’, 5’-GAGGUUAUUGAACGACUCA-3’) non-targeting scrambled siRNA (Sigma, SIC001-1NMOL) for 48-72 hours using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... and 4.5 µL aliquots were combined at different times with 0.5 µL of 10X GODCAT visualization buffer (50 mM Tris-HCl pH 7.5; 2 mg/mL Glucose Oxidase (SIGMA cat. no. G2133); 350 ng/mL Catalase (SIGMA cat ...
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bioRxiv - Neuroscience 2021Quote: ... ∼3-5 days post-eclosion female flies were loaded into locomotor tubes with 5% sucrose / 2% agar food supplemented with 500uM mifepristone (RU+ food) (Sigma-Aldrich M8046). Sleep was recorded from counts of beam breaks on single-beam DAM2 monitors (Trikinetics) ...
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bioRxiv - Cell Biology 2022Quote: ... around 150 young adults were transferred to fresh NGM plates containing 49.5 μM 5-fluoro-2’-deoxyuridine (FUDR, Sigma-Aldrich, MO, USA) to prevent progeny production ...
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bioRxiv - Genetics 2021Quote: ... the worms were transferred onto NGM plates treated with 50 μM of 5-fluoro-2’-deoxyuridine (FUdR, Sigma, St. Louis, MO, USA). Worms were maintained on these plates at 20°C until they were used for experiments at day 1 ...
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bioRxiv - Plant Biology 2019Quote: ... the pellet (partially purified ribosome extract) was resuspended in 2 ml of a solution that consisted of 5 mM DTT (Sigma Aldrich D0632) and 1.5 M sucrose ...
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bioRxiv - Neuroscience 2019Quote: ... BW723C86 (α-methyl-5-(2-thienylmethoxy)-1H-Indole-3-ethanamine monohydrochloride) and a primary antibody raised against β-actin were purchased from Sigma (USA). Other primary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Pre-treated cells were incubated with media containing 20 μM 5-chloro-2’-deoxyuridine (CldU) (Milipore Sigma; Cat# C6891, St. Louis, MO) for 30 m at 37°C before being washed and incubated in media containing 100 μM 5-iodo-2’-deoxyuridine (IdU ...
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bioRxiv - Neuroscience 2020Quote: ... coated with poly-d-lysine (5 mg/50 ml distilled water for 2 hours, PDL; 135 kDa; Sigma-Aldrich, St. Louis, MO) or onto poly-d-lysine-coated BD BioCoatTM (BD Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... age-synchronized animals at the L4 stage were collected in M9 buffer and plated on NGM plates containing 5-fluoro-2’-deoxyuridine (FUDR, Sigma, 50 μM) to prevent egg-laying (84).
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bioRxiv - Neuroscience 2020Quote: ... then incubated with the primary antibody (rat anti-serotonin, Chemicon MAB352, 5/1000; rabbit anti-tyrosine hydroxylase, Sigma Aldrich SAB2701683, 2/1000) in buffer (1% sheep serum ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed in phosphate buffer saline with 0.5% Triton-x (PBST) and incubated with 20% heat-inactivated normal goat serum in PBST for 2 hours (NSS; Sigma-Aldrich, Corp.). Primary antibodies were applied overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse mAbs anti-Vinculin (hVIN-1) and anti-α Tubulin (clone B-5-1-2) and rabbit polyclonal anti-Paxillin (HPA051309) were from Sigma-Aldrich and were diluted 1:400 ...
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bioRxiv - Plant Biology 2020Quote: ... and 10 μCi [γ32P]-ATP in reaction buffer (25 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 0.5 mM CaCl2, 2 μM CaM [Sigma-Aldrich], 1 mM DTT) at 30°C for 30 min to perform the vitro kinase assay ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM 2-mercaptoethanol) and concentrated up to 1 nmol/ml (1A260 = 69 pmol) using Amicon Ultra centrifugal filters (Millipore, MWCO 100,000). Further ...
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bioRxiv - Biophysics 2020Quote: ... The DNA construct is attached to the streptavidin coated beads by incubating 0.5 μl of picomolar DNA stock solution and 2 μl beads in 250 μl 1x phosphate buffered saline (PBS, Sigma-Aldrich, USA) for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... and 500 µL of each lysate sample was incubated for 2 h at room temperature with 10 µL of 5 mM biotin picolyl azide (in DMSO) (Sigma Aldrich 900912), 10 µL of 50 mM TCEP (in water) ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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bioRxiv - Microbiology 2021Quote: The pieces of residue (each weighing approximately 5 g) were soaked in 2 mL of phosphate-buffered saline (PBS, Sigma-Aldrich P4417) supplemented with Tween 20 (0.05% v/v ...
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bioRxiv - Neuroscience 2021Quote: ... animals from all groups were injected intraperitoneally with the thymidine analogous 5-bromo-2’-deoxyuridine or bromodeoxyuridine (BrdU, 50 mg/kg; Sigma-Aldrich, US) that is incorporated in the DNA during the S-phase ...
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bioRxiv - Biochemistry 2020Quote: ... 5 μl 1x PBS and 2 μl (1 U) PNGase F (from Elizabethkingia meningoseptica, expressed in E. coli, Sigma Aldrich F8435-50UN) were added to 40 μl of SEC purified YFP-S-RBD (0.24 mg/ml protein concentration) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reactions were quenched by adding 5 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) (Sigma-Aldrich, E3889) and pelleted afterwards ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were washed in phosphate buffer saline with 0.5% Triton-x (PBST) and incubated with 20% heat-inactivated normal sheep serum in PBST for 2 hours (NSS; Sigma-Aldrich, Corp.). Primary antibodies were applied overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 24h post-plating the cardiomyocytes were fed with fresh medium supplemented with 2% FCS and with 10-5 M Isoproterenol (Sigma I-2760). To induce proliferation ...
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bioRxiv - Immunology 2021Quote: ... and homogenized with a 2 ml dounce grinder for 5 strokes with A motor and at least 20 strokes with B motor (Sigma-Aldrich D8938). The lysates were filtered through 100 µm and 50 µm cell strainers (CellTrics filters 04-004-2328 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and positively transduced cells were selected using blasticidin (2 µg/ml Sigma-Aldrich, Poland). Primary human epithelial cells seeded on 10 cm2 dishes were cultured in BEGM medium and transduced with lentiviral particles harboring shRNA against KLK13 (a set of 3 ...
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bioRxiv - Plant Biology 2021Quote: ... 10 mM MgCl2, 250 mM sucrose, 0.5% Triton X-100, 5 mM 2-mercaptoethanol and 1X protease inhibitor cocktail [Millipore-Sigma, Cat# P9599]), and carefully overlaid on top of 500 μL of nuclear resuspension buffer 3 (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... for 36-48 h were plated on the SNS imaging plate along with 2 or 5 unit/mL of PI-PLC (P8804, Sigma Aldrich, USA) in complete growth medium for 1 h and then stained with phalloidin-Alexa 405 prior to imaging ...
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bioRxiv - Cell Biology 2021Quote: ... 1 volume of AluI-stock was added to 2 volumes of 5 mg ml−1 fluorescein (FITC) labelled 500 kDa dextran fraction (Sigma-Aldrich, FD500S) dissolved in injection buffer (50 mM K-HEPES ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% galactose [Sigma-Aldrich #G0625] ...
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bioRxiv - Immunology 2022Quote: ... we used a synthetic cathepsin C inhibitor (IcatCXPZ-01; (37) diluted in 10 % (2-Hydroxypropyl)-β-cyclodextrin (CAS: 128446-35-5; C0926; Sigma-Aldrich) suspended in citrate buffer 50 mM at pH=5 (vehicle ...
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bioRxiv - Neuroscience 2023Quote: ... equivalent to about 5 mg/kg/day) was prepared in vehicle made of 0.45% (2-hydroxypropyl)-beta-cyclodextrin (β-CD; both from Sigma-Aldrich, France), and administered in drinking water for 28 days ...
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bioRxiv - Neuroscience 2022Quote: Snap frozen retinal samples from 5 older and 5 young primates from both central macular and peripheral regions were homogenized in 2% sodium dodecyl sulfate (SDS, Sigma Aldrich, UK) with protease inhibitor cocktail (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Yhb1-TAP and α-tubulin proteins were detected using the polyclonal anti-mouse IgG antibody (ICN Biomedicals) and monoclonal anti-α-tubulin antibody (clone B-5-1-2; Sigma-Aldrich), respectively ...
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bioRxiv - Physiology 2023Quote: Mice were lightly and briefly (< 5 min) anesthetized with 2% isoflurane and injected with 40% w/v PEG-8000 (Sigma-Aldrich, 89510) in sterile 0.9% NaCl subcutaneously using a 28G insulin syringe ...
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bioRxiv - Cell Biology 2024Quote: ... Worms were synchronized by bleaching and Day 1 adults were plated on NGM plates containing 100 µM 5-Fluoro-2′-deoxyuridine (FuDR) (Sigma Cat. F0503) with or without 4 mM methyl viologen dichloride hydrate (paraquat ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3 times with 1x PBS and incubated for 2 hours with 5% goat serum (NGS -Sigma-Aldrich, G9023) in 1x PBS with 0.3% Triton (Sigma-Aldrich ...