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Citations for Millipore Sigma :
3301 - 3350 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... 100 µl homogenate were added to 400 µl of ice-cold methanol/water 4:1 (v/v) / 0.2% formic acid and isotope-labeled internal standards (10 µl 2 µM 13C3,15N-3-hydroxykynurenine (Sigma-Aldrich) and 10 µl 2 µM D5-kynurenic acid (Eurisotop)) ...
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bioRxiv - Biochemistry 2021Quote: ... CGI-99(2-101)-containing peak fractions were concentrated using a centrifugal filter (Amicon Ultra, MWCO 3 kDa, Sigma) to 24 mg ml−1 ...
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bioRxiv - Molecular Biology 2021Quote: ... gallinamide A-bound cruzain was concentrated to 10 mg/mL and the activation buffer was exchanged to 2 mM Bis-Tris pH 5.8 using a centrifugal filter with molecular weight cut-off of 3 kDa (Millipore). For cruzain-gallinamide A analog complexes ...
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bioRxiv - Microbiology 2020Quote: ... 2 mL of 0.3% agarose in DMEM supplemented with 2% FBS or 3 mL of 0.6 or 1.2 percent microcrystalline cellulose (MCC; Sigma 435244) in serum-free DMEM was added ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (60) ...
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bioRxiv - Immunology 2023Quote: ... RIPA 400 mM NaCl, (3) Sarkosyl Buffer (2 mM EDTA, 50 mM Tris-HCl, 0.2% sarkosyl (Lauroylsarcosine sodium salt (Sigma)) ...
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bioRxiv - Microbiology 2023Quote: ... and AP2XII-1-AID-HA /AP2XI-2-mAID-MYC was achieved with 3-indoleacetic acid (IAA, Sigma-Aldrich # 45533). A stock of 500 mM IAA dissolved in 100% EtOH at a ratio of 1:1,000 was used to degrade mAID-tagged proteins to a final concentration of 500 μM ...
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bioRxiv - Molecular Biology 2023Quote: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium Bromide (MTT) cell growth kit (CT02, Millipore, MA, USA) was used for measuring cell viability ...
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bioRxiv - Cell Biology 2023Quote: ... 1 3 v/v) at 80°C for 2 h after addition of 50 nmol 5α-Cholestane (Sigma, C8003) as internal standard for neutral sterol analysis ...
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bioRxiv - Microbiology 2023Quote: The effect of drug treatments on cell viability was assessed using the MTT [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay (the MTT powder was obtained from Sigma Aldrich). VTN or Huh-7 cells were seeded the day before treatment at a concentration of 7L×L103 cells per well in DMEM supplemented with 10% FBS and 1% Pen/Strep ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed in 80% ethanol for 2-3 minutes and differentiated in 0.1% lithium carbonate (Sigma #62470) for 45-60 seconds followed quickly by washes in distilled water to stop the differentiation ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was mixed at a 3:2 ratio with a crowding buffer [20% dextran (from Leuconostoc spp., Mr 450,000-650,000; Sigma 31392) and 1 mM DTT in 50 mM Tris-HCl pH 7.4] ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL aliquots were taken from the main culture for chemical fixation with 3% paraformaldehyde (PFA, Sigma-Aldrich, #F8775) at 20 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... The PDMS microstructures were then rinsed with 2:3 HEPES1/ethanol and with ultrapure water (Milli-Q, Merck-MilliPore). PVP (1.3 MDa ...
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bioRxiv - Neuroscience 2024Quote: ... cells grown in 96-well plates were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, Hungary) in a final concentration of 200 μg/ml ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Sigma-Aldrich (St Louis, MO). Phalloidin-iFluor 594 was purchased from Abcam ...
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bioRxiv - Microbiology 2024Quote: ... after which the remaining supernatant (2 x 45ml) was collected and concentrated over a 3 kDa Amicon filter (Millipore). The residue was suspended in a volume of 900 µl ...
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bioRxiv - Cell Biology 2023Quote: ... Treatment of mice were performed with a single intraperitoneal injection of either vehicle (corn-oil) or 3 mg/kg 1,4-bis-[2-(3,5,-dichloropyridyloxy)] benzene (TCPOBOP, T1443, Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... in 0.4% Triton X-100 and 2% NHS PBS solution during 3 h and then coverslipped with Mowiol (Sigma). After confocal imaging ...
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bioRxiv - Neuroscience 2022Quote: ... On selected sections, immunoreactivities were tested: SATB1 (mouse, 1:500, SantaCruz) and GluR 2/3 (rabbit, 1:500, Millipore) were diluted in 0.1 M PB and incubated overnight at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... washed in PBS and incubated in buffer A (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Plant Biology 2022Quote: ... After addition of 10μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) solution (5mg/ml, Sigma-Aldrich, USA) the cells were incubated in darkness for another 4 hrs ...
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bioRxiv - Microbiology 2022Quote: The effect of CAPG depletion on viability of KO and siRNA-treated cells was assessed at several timepoints using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay (11465007001; Sigma-Aldrich) per manufacturer directions ...
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bioRxiv - Neuroscience 2022Quote: Recordings were then performed in oxygenated aCSF superfused at a rate of 2-3 ml/min and supplemented with 100 µM picrotoxin (Alomone Labs) and 0.5-1 µM Strychnine (Sigma). The internal solution was the same as above with 2-4 mM QX314 to block Na+ channels from driving AP firing ...
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bioRxiv - Cancer Biology 2022Quote: ... Five thousand cNF-derived primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated opaque 96-well plates in SCM without IBMX with Forskolin and maintained at 37ºC under a 10% CO2 atmosphere for 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: One hundred thousand primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated 12-well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... One hundred thousand primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated 12-well plates in SCM without IBMX ...
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bioRxiv - Biophysics 2022Quote: ... Concentrated Cav1.2(ΔC)/Cavβ3/Cavα2δ-1 sample was immediately incubated with gabapentin (final concentration 2 mg ml-1, 11.7 mM) (Sigma-Aldrich) on ice for four hours prior to cryo-EM sample preparation ...
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bioRxiv - Developmental Biology 2023Quote: ... unlysed embryos were cultured during 2 to 3 hours in 40 µL micro drops of TCM199 (Sigma-Aldrich, M4530) + 10% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5mg/ml of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich®, St Louis, MO, USA) was added and incubated at 37°C for 3h ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (Pizzato Scomazzon et al. ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 99.7% pure), Bisphenol TMC (Catalog #, 52746-49-3) and 2′,7′-Dichlorodihydrofluorescein diacetate (Catalog #, D6883) were purchased from Millipore Sig ma (MO ...
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bioRxiv - Molecular Biology 2024Quote: ... They were grown for 2 days in ENR media containing CHIR-99021 (Sigma-Aldrich, #252917-06-9; 3 μM) and Y-27632 (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... or (E)-2-Benzylidene-3-(cyclohexylamino)-2,3-dihydro-1H-inden-1-one (BCI, DUSP 1/6 inhibitor, Cat# 317496, Sigma) at different concentrations as indicated for each experimental condition ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 x in TBS-Tween (0.1%) + 2% BSA before incubation in TRITC conjugated anti-Mouse secondary antibody (Sigma) at 1:1000 concentration for 1 hr in the dark ...
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bioRxiv - Biophysics 2024Quote: ... the cleaned quartz slides were incubated in a 2% (v/v) solution of (3-aminopropyl)triethoxysilane (a3648, Sigma-Aldrich) for 20 minutes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Plasmids encoding GST-F-box fusion proteins were retransformed in Rosetta 2(DE3) Singles Competent Cells (Novagen, 71400-3), as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Live larvae (3-10 dpf) were immobilized in tricaine and mounted in 1-2% low gelling agarose (Sigma: A9414) on 0.17 mm coverslips and imaged on a Stellaris DMI8 laser scanning confocal microscope at 1024×1024 resolution using the same acquisition settings with a 25x water immersion objective.
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bioRxiv - Cell Biology 2021Quote: ... 3 (Sigma) in de-ionised (DI ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... 3 (Sigma) as counterstain.
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bioRxiv - Immunology 2021Quote: ... antibody (1:1,200) (eBioscience) and for 5 min at 4 °C with the DAPI viability dye solution (1 µg/mL) (Sigma) to identify dead cells ...
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bioRxiv - Immunology 2020Quote: ... mosquitoes were kept at 26.0°C (± 0.5°C) in an ultrasonic humidity cabinet and provided with 8 % Fructose and 0.05 % 4-Aminobenzoic acid (both Sigma) feeding solution from this point forward ...
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bioRxiv - Genomics 2020Quote: ... cleared by centrifugation at 1500rpm for 5 minutes at 4⁰C and then passed through a 0.45um pore PVDF Millex-HV (Millipore). Lentivirus was aliquoted and stored at −80⁰C for future use.
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bioRxiv - Microbiology 2021Quote: ... 35x volume per mg) by vortexing vigorously for 5 min using 4 mm glass beads (ColiRollersTM Plating beads, Novagen), followed by incubation in a sonication bath for 10 min and shaking at 1,200 rpm for 10 min in a Thermomixer with a thermo block for reaction tubes ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed in 10% buffered formalin (4% formaldehyde) for 5 min at room temperature and permeabilized for 30 min in PBS containing 0.1% saponine (Sigma) and 1% FBS (HyClone) ...
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bioRxiv - Microbiology 2020Quote: ... and membrane blocked overnight while shaking at 4°C in 5% milk (Marvel) containing 0.077% sodium azide (Sigma Aldrich). Primary antibodies were diluted in 5% milk/PBS/0.1% TWEEN 20 at concentrations as follows ...
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bioRxiv - Plant Biology 2021Quote: ... Pellet was washed twice with acetone at maximum speed (13,000xg) for 5 minutes at 4◦C and then air dried and resuspended in 50 mM ammoniumbicarbonate (ABC) (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated at 37 °C and 5% CO2 for 4 days before addition of resazurin (Sigma Aldrich) to a final concentration of 20 μg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... gonads were dissected from 4 and 5 day old female jellyfish in 400 µM menthol in seawater (Sigma-Aldrich, #M2772 ...
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bioRxiv - Bioengineering 2022Quote: ... the medium was changed to RPMI + B27-I with 5 μM Wnt inhibitor IWP-4 (SML1114, Sigma-Aldrich, USA) and incubated for 48 hours ...