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Citations for Millipore Sigma :
3301 - 3350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and 0.57 µg VSV-G plasmid (pMD2.G) using 26 µL of XtremeGene HP (6366244001, Sigma Millipore) in 1 mL of Optimem (11058-021 ...
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bioRxiv - Biochemistry 2024Quote: ... The cell lines were tested for mycoplasma contamination using the LookOut® Mycoplasma PCR Detection Kit (Sigma Millipore) and were found to be negative for mycoplasma.
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bioRxiv - Biochemistry 2024Quote: ... Thioflavin T (ThT) dye was purchased from Sigma-Aldrich. Acrylamide/bis-acrylamide 29:1 40% (BioLab) ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 volume ethanol (Sigma, E7023) to perform EtOH precipitation,[51] and the dsDNA pellet was resuspended in 5 mM Tris in ddH2O ...
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bioRxiv - Biochemistry 2024Quote: ... Bis-Tris Hydrochloride (Sigma Aldrich), and sodium chloride (BioLab ...
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bioRxiv - Biochemistry 2024Quote: ... were used for the transformation of pET-28b(+) plasmids (Novagen). Phenylmethylsulfonyl fluoride protease inhibitor (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... N’-Tetramethylethylenediamine (Sigma Aldrich) were used for gel preparation ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Biochemistry 2024Quote: ... The cell lines were tested for mycoplasma contamination using the LookOut® Mycoplasma PCR Detection Kit (Sigma Millipore) and were found to be negative for mycoplasma.
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bioRxiv - Biochemistry 2024Quote: ... and 0.57 µg VSV-G plasmid (pMD2.G) using 26 µL of XtremeGene HP (6366244001, Sigma Millipore) in 1 mL of Optimem (11058-021 ...
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bioRxiv - Biochemistry 2024Quote: ... Phenylmethylsulfonyl fluoride protease inhibitor (Sigma-Aldrich); guanidinium HCl (SPECTRUM ...
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bioRxiv - Biochemistry 2024Quote: ... The PicoProbeTM assay (Sigma Millipore) and analysis were performed according to the manufacturer’s protocols using the picomole standard curve (n = 1) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated to 10 mL using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore) and loaded into SnakeSkin Dialysis Tubing ...
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bioRxiv - Bioengineering 2024Quote: ... The antibody solution was then concentrated on Amicon Ultra-4 centrifugal filters with 10 or 50 kDa molecular weight cutoff (Millipore) to 1-4 mg/ml in PBS.
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bioRxiv - Biochemistry 2024Quote: ... Proteins were concentrated using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore). Protein and ligand samples were degassed prior to each ITC experiment ...
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bioRxiv - Biochemistry 2024Quote: ... Low passage HeLa cells (ATCC, CCL-2) were transduced with the lentivirus in media containing 8ug/mL polybrene (Sigma Aldrich, TR-1003-G) for 48 hours ...
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bioRxiv - Biochemistry 2024Quote: ... 0.2 mM inosine nucleoside (Sigma, I4125-1G); m6A ...
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bioRxiv - Biochemistry 2024Quote: ... with 1x MEM Non-essential Amino Acid (NeA) Solution (Sigma Cat# M7145). After 16-24 hours ...
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bioRxiv - Biochemistry 2024Quote: ... samples were then transferred directly into blocking buffer (4 % BSA (Sigma Cat# 10735086001) plus 3 % normal goat serum (Jackson Immunoresearch #005000121 ...
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bioRxiv - Biochemistry 2024Quote: HeLa cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Nakalai Tesque) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich) and 1% penicillin-streptomycin (Nakalai Tesque ...
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bioRxiv - Biochemistry 2024Quote: ... or OrigamiB(DE3) (Novagen) and the cells were grown in M9 media at 37 °C until the culture reached an OD600 of ∼ 0.8-1 ...
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bioRxiv - Biochemistry 2024Quote: ... MilliQ water was produced by Milli-Q Direct (Merck Millipore, MA, USA). Universal Hood II-Gel Doc system (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... 6-Chlorohexanol (Sigma-Aldrich, C45008), Trypsin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed from the grids by gently touching Whatman filter paper (Sigma, WHA1001329) to the edge ...
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bioRxiv - Biochemistry 2024Quote: ... The BCL11A enhancer DHS +58 on-target region was amplified with KOD Hot Start DNA Polymerase (EMD-Millipore, 71086-31) and corresponding primers (forward primer 5’-AGAGAGCCTTCCGAAAGAGG-3’ and reverse primer 5’ GCCAGAAAAGAGATATGGCATC-3’) ...
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bioRxiv - Biochemistry 2024Quote: ... A total of 1 µl of locus specific PCR product was used for indexing PCR using KOD Hot Start DNA Polymerase (EMD-Millipore, 71086-31) and TruSeq i5 and i7 indexing primers (Illumina ...
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA was extracted from single colonies after culturing in TYG using the GenElute™ Bacterial Genomic DNA kit (Sigma) and screened for tag insertions by PCR using primers flanking various att sites ...
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bioRxiv - Biochemistry 2024Quote: TLC analysis of sugars was performed using silica TLC plates (Sigma) as previously described [65] ...
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bioRxiv - Biochemistry 2024Quote: ... Transferred PVDF membranes were blocked in 5% milk powder in 0.2% Tween-TBS overnight at 4°C and immunoblotted with mouse anti-FLAG-HRP (Sigma) conjugated primary antibody ...
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bioRxiv - Biochemistry 2024Quote: ... used to grow transformants contained 1.92 g.L-1 (w/v) Yeast Synthetic Drop-out Medium Supplements without Uracil (Sigma-Aldrich, Y1501), 6.7 g.L-1 Yeast nitrogen base without amino acid (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Anti-FLAG M2 magnetic beads (40 μL slurry; Sigma-Aldrich) were initially washed twice in 1.0 mL IP buffer supplemented with 0.05% NP40 and then kept in 0.3 mL IP buffer supplemented with 2 μg 1xFLAG peptide ...
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bioRxiv - Biochemistry 2024Quote: ... Electroporated cells were cultured in erythroid differentiation medium (EDM) consisting of IMDM (GibcoTM, 12440061) supplemented with 330 µg/ml of Holo-Human Transferrin (Sigma-Aldrich, T0665-1G), 10 µg/ml of recombinant human insulin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were acidified with formic acid (pH < 3) and then desalted using C18 ZipTips (Millipore) according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA was extracted from 2 ml of overnight culture from each time point using the GenElute™ Bacterial Genomic DNA kit (Sigma) and 10 ng analysed by qPCR using a Light Cycler 480 real-time PCR system (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... 6.7 g.L-1 Yeast nitrogen base without amino acid (Sigma-Aldrich, Y0626), and 20 g.L-1 glucose.
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bioRxiv - Biochemistry 2024Quote: ... monosaccharides and PNP-linked monosaccharides were from Sigma. T-antigen (Galβ1,3GalNAcα1-Ser ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant plasmids were transformed into BL21 (DE3) or BL21 (DE3) Tuner cells (Novagen) and were incubated in 1L LB in 2L conical flasks at 37°C ...
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bioRxiv - Biochemistry 2024Quote: Glibenclamide was purchased from Sigma-Aldrich. Poloxamer-188 ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg/ml of recombinant human insulin (Sigma-Aldrich, 19278-5ML), 2 IU/ml heparin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma) in washing solution for 1h ...
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bioRxiv - Biochemistry 2024Quote: ... 2 IU/ml heparin (Sigma-Aldrich, H3149), 5% of human solvent detergent pooled plasma AB (Rhode Island Blood Center ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR products were cloned into pET28 (Novagen) or pRSETA (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... plasmids were transfected using GeneJuice (MilliporeSigma/ Novagen) transfection reagent as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... IgA1 was pre-treated with a sialidase from Clostridium perfringens (Sigma), BT4241-GH2 β-galactosidase (0.5 µM ...
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bioRxiv - Biochemistry 2024Quote: ... EDM was supplemented with 10-6M hydrocortisone (Sigma-Aldrich, H0135), 100 ng/ml human SCF (CellGenix ...
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bioRxiv - Biochemistry 2024Quote: ... or minimal medium (MM) containing 0.5-1% (w/v) of an appropriate carbon source plus 1.2 mg/ml porcine hematin (Sigma) as described previously [22] ...
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bioRxiv - Biochemistry 2024Quote: ... IgA deglycosylation was monitored using a biotin–conjugated Helix aspersa agglutinin (HAA) (Sigma) that binds specifically to GalNAc ...
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bioRxiv - Biochemistry 2024Quote: ... anti-LC3B (L7543) and anti-β-Actin were purchased from Sigma (St. Louis, MO, USA); Anti-BRD4 (13440) ...
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bioRxiv - Biochemistry 2024Quote: ... Anti-FLAG M2 Affinity Gel (Sigma cat# A2220) was used to capture CXCR4EM-containing species ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were incubated with a FITC-labeled anti-FLAG antibody (Sigma, Cat #F4049 ...