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Citations for Millipore Sigma :
3151 - 3200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Mouse zygotes were isolated in FHM under mineral oil and cumulus cells were removed with hyaluronidase (Sigma-Aldrich; H4272). Mouse embryos were cultured in pre-equilibrated Global Media (Cooper Surgical ...
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bioRxiv - Cell Biology 2024Quote: ... Evans Blue was extracted from tissue pieces for 48 hours in N,N-Dimethylformamide (Sigma, cat. #D4551) at 55°C ...
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bioRxiv - Cell Biology 2024Quote: ... the supernatant discarded and the cells re-suspended in 500 µl of azide labeling solution (1X PBS, 1 mM CuSO4 (Sigma-Aldrich, C1297-100G), 100 µM Fluorescein Azide (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 IU of human chorionic gonadotrophin (HCG; Sigma-Aldrich) was administered ...
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bioRxiv - Cell Biology 2024Quote: ... chromatin from different treatment groups was cross-linked using 1% formaldehyde (Sigma-Aldrich). Chromatin from 1x106 cells was incubated with IgG (ab171870) ...
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bioRxiv - Cancer Biology 2024Quote: ... Signal detection was conducted with 3,3′-Diaminobenzidine (Sigma-Aldrich, cat. D4293). Counterstaining was conducted with Mayer’s hematoxylin.
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bioRxiv - Molecular Biology 2024Quote: ... and transferred onto 0.45µm PVDF membrane (Immobilon, Millipore/Merck) via a wet transfer system with 1X NuPAGETM transfer buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was resuspended in 500 µl of “working solution”: Liberase™ TH 0.1 WU/mL (Sigma, St. Louis, MO, USA); DNAse1 50 U/mL (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... antigenic type MITat 1.2, clone 221) were cultured in HMI-9 medium (Hirumi and Hirumi, 1989) supplemented with 10 % foetal bovine serum (Sigma-Aldrich, USA), 100 U/ml penicillin and 0.1 mg/ml streptomycin ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 mM 3-isobutyl-1-methylxanthine (Sigma-Aldrich, no. I5879-5g) and 167 nM insulin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were then transferred onto Immobilon-P PVDF membranes (#IPVH00010; Millipore) for immunoblotting ...
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bioRxiv - Cell Biology 2024Quote: ... and 167 nM insulin (Sigma-Aldrich, no. I9278-5ml). The cells were maintained in the basic culture medium supplemented with 167 nM insulin until harvesting.
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bioRxiv - Cell Biology 2024Quote: ... 100 μg/ml Emetine dihydrochloride (Sigma) was added to inhibit cytosolic translation ...
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bioRxiv - Molecular Biology 2024Quote: ... 4.5 g/L glucose consisting of ∼20% [U-13C6]glucose and of 80% non-labelled glucose was added into glucose-free DMEM supplemented with 2 % horse serum (Sigma-Aldrich, H1138, NY, USA). Cells were incubated for 24 h with isotope-enriched media on day 3 post differentiation after which the media was collected ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... IP were performed overnight at 4°C using 1.5 mg of pre-coated epoxy Epoxy Dynabeads (M-270) with 1.5 μl of anti-FLAG (Sigma, F1804) per reaction ...
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bioRxiv - Molecular Biology 2024Quote: cDNAs of BTB domains were PCR-amplified using corresponding primers (Supplementary Table S1) and cloned into modified pET32a(+) vector (Novagen) encoding TEV protease cleavage site after 6xHis-tag and Thioredoxin ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM dithiothreitol and 2% (v/v) protease inhibitor cocktail (Sigma, St Louis, MO). Protein extracts were incubated at 65 °C for 10 min and then centrifuged at 13,000 g for 1 min at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with polybrene (Sigma-Aldrich, no. TR-1003-G) at a final concentration of 10 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... TF expression was induced by Doxycycline (2 µg/mL; Sigma-Aldrich, no. D9891-1G) treatment during 5 days in cells placed in a basic culture medium refreshed every 48 h ...
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bioRxiv - Cell Biology 2024Quote: ... which was composed of the basic culture medium and MDI cocktail containing 1 µM dexamethasone (Sigma-Aldrich, no. D4902-25MG), 0.5 mM 3-isobutyl-1-methylxanthine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... for lipids and DAPI (1:5000; Sigma-Aldrich, no. D9542-1MG) for nuclei ...
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bioRxiv - Cell Biology 2024Quote: Uninduced and induced samples were treated with either 0.2 μM or 2 μM Latrunculin A in DMSO (Sigma, CAS 76343-93-6) up to 4 h at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were picked up and deposited on formvar- and carbon-coated grids using 2.3 M sucrose and 1.8 % (v/v) methylcellulose (MC, Sigma M6385 25 centipoises) mixed 1:1.
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bioRxiv - Cell Biology 2024Quote: ... pre-treated with Concanavalin A (ConA): 75 μL of 0.2 mg/μL ConA (Sigma-Aldrich) was added to each well and incubated at room temperature for 15 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... and labeled with digoxygenin (DIG) NTPs (Sigma/Millipore #11277073910) or fluorescein NTPs (Sigma/Millipore #11685619910) ...
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bioRxiv - Cell Biology 2024Quote: ... GFP-PMCA4bLA and PMCA4-specific shRNA expressing MCF-7 lines were mixed with cold Matrigel (Corning Matrigel, DLW356231, Sigma-Aldrich) and 15 µl of cell-Matrigel mixtures were added to removable 8-well chambers (80841 ...
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bioRxiv - Cell Biology 2024Quote: ... cell pellets were immersed in a thiocarbohydrazide (TCH, Sigma-Aldrich, Cat. # 223220) solution for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were incubated in blocking solution containing 2 mg/ml bovine serum albumin (A7030, Sigma-Aldrich), 1% gelatin from cold water fish skin (G7765 ...
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bioRxiv - Molecular Biology 2024Quote: ... phospho-histone H3 (Ser10)(06- 570; Sigma-Aldrich). ATAD5 antibodies were raised in rabbits against a synthetic peptide containing aa 183 to aa 210 of human ATAD5 ...
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bioRxiv - Molecular Biology 2024Quote: ... with 10 % fetal bovine serum and 1 % Penicillin-Streptomycin (P4333; Sigma-Aldrich) at 37℃ in a 5 % CO2 atmosphere ...
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bioRxiv - Cell Biology 2024Quote: ... An equal volume of phenol-chloroform pH=8 (Sigma-Aldrich) was added ...
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bioRxiv - Cell Biology 2024Quote: ... b) Minimal Induction (MI) (Hilgendorf et al., 2019) containing 0.4 µg/ml insulin (Sigma), 0.1 µM Dexamethasone (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... 5% ChemiBLOCKER (Merck Millipore) in 0.1 M NaP ...
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bioRxiv - Cell Biology 2024Quote: ... and 20 µM 3-isobutyl-1-methylxanthine (IBMX; Sigma); c ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were fixed with 0.5% glutaraldehyde for 15 min and stained with 0.1% Crystal Violet solution (Sigma) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... and 17 mM D-pantothenate (Sigma) at 37°C with 5% CO2 in a cell culture incubator.
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bioRxiv - Cell Biology 2024Quote: ... and 15 kU/mL DNAse I (PanReac AppliChem) in 0.5% bovine serum albumin (BSA; Sigma) in phosphate-buffered saline (PBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and lycopene (≥ 90%) were obtained from Sigma Aldrich Chemie GmbH and Zeaxanthin (≥ 90% ...
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bioRxiv - Cell Biology 2024Quote: ... This solution contained 30 µl of scrambled or 10 µl VPS34-targeting shRNA per target sequence to a total of 30 µl and was supplemented with 6x polybrene to reach a final concentration of 6 µg / ml (Sigma Aldrich, Cat. # TR-1003-G) in a complete medium ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were mechanically lysed using a solution composed of RIPA buffer (Sigma-Aldrich) and 1/100 protease inhibitors (Calbiochem) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Chlorophyll a (≥ 95%) and b (≥ 95%) were obtained from Sigma Aldrich Chemie GmbH ...
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bioRxiv - Cell Biology 2024Quote: ... the non-specific sites were saturated by incubation at room temperature with agitation for 90 minutes in a solution of 1X PBS/5% milk/0.2% Tween (TWEEN©20, Sigma-Aldrich). The membranes were then incubated overnight at 4°C in a humidity chamber with the primary antibody diluted in PBS 1X/milk 5%/Tween 0.2% ...
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bioRxiv - Cell Biology 2024Quote: ... was conjugated to fatty-acid-free BSA (Sigma-Aldrich, Cat. # A7030) by incubating at 37 °C for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were expressed in the BL21 strain from Escherichia coli at 37°C in LB medium (Sigma) supplemented with 100 µg/mL ampicillin until OD600 between 0.4 and 0.5 ...
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bioRxiv - Cell Biology 2024Quote: ... gels were overlaid with 5 x inhibitor / vehicle solution and 5x fMLP (Sigma-Aldrich, Cat # F3506 ...
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bioRxiv - Cell Biology 2024Quote: ... were coated with 150 µl of human plasma fibronectin (10 µg / ml; EMD Millipore, Cat. # FC010) in PBS and incubated at 37 °C for 2 h ...
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bioRxiv - Genetics 2024Quote: ... puromycin (Sigma #P8833), NAC (Sigma #A7250) ...
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bioRxiv - Genetics 2024Quote: ... incubated with 10 μM DHE (Sigma #D7008) for 30 min on a tube rotator ...
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bioRxiv - Genetics 2024Quote: ... CHX (Sigma #1810), puromycin (Sigma #P8833) ...