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Citations for Millipore Sigma :
3501 - 3550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The stained filters were compared to a standard curve developed using xanthan gum (Sigma-Aldrich), and hence TEP determinations were expressed as standardized xanthan gum equivalents (XGeq ...
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bioRxiv - Biochemistry 2024Quote: Concentrations of biogenic and lithogenic silica (bSi and lSi) were determined by filtering subsamples onto 0.6-μm pore size polycarbonate membrane filters (47 mm PC, Isopore, Millipore), which were frozen at sea in cryovials at −20°C ...
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bioRxiv - Biophysics 2024Quote: ... Trizma hydrochloride (≥99%, Sigma-Aldrich), Trizma base (≥99.9% ...
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bioRxiv - Biophysics 2024Quote: ... 2.5% glycerol (v/v)) using a 10 kDa MWCO Amicon Ultra centrifugal filter (Millipore). Purified Trx-LqhαIT fusion protein concentration was determined by 280 nm absorbance on a Nanodrop 2000 spectrophotometer (Thermo ...
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bioRxiv - Biochemistry 2024Quote: ... General laboratory chemicals were supplied by Sigma Aldrich, Merck or Thermofisher unless otherwise stated.
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bioRxiv - Biophysics 2024Quote: ... each substrate was sonicated in toluene (cat. No. 244511, Sigma Aldrich) and absolute ethanol (99% ...
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bioRxiv - Biophysics 2024Quote: ... 2 mM L-glutamine (cat. No. G7513 Sigma-Aldrich, Darmstadt, Germany) and antibiotics - 100 µg/ml streptomycin with 100 units/ml penicillin (cat ...
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bioRxiv - Biophysics 2024Quote: ... using a Guava benchtop Flow Cytometer (Millipore, Sigma).
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bioRxiv - Biophysics 2024Quote: ... The experiment involved growing cells in three different glucose concentrations: normoglycemic control (NG) with 5 mM D-glucose (cat. No. G8270 Sigma-Aldrich, Darmstadt, Germany), medium hyperglycemia conditions (MDM ...
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bioRxiv - Biophysics 2024Quote: The insulin-releasing pancreatic β-cell line (1.1B4, Merck KGaA, Sigma Aldrich, Darmstadt, Germany) was cultured in a 175 cm2 cell culture bottles and maintained in RPMI 1640 medium (cat ...
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bioRxiv - Biophysics 2024Quote: ... The PRP was added with 1.2 U/mL apyrase (Sigma-aldrich, St. Louis, MO) and 10 μM Prostaglandin E1 (Cayman chemical company ...
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bioRxiv - Biophysics 2024Quote: ... The DNA concentration in BSM samples was calculated from the calibration curve which was generated with using known concentrations of DNA solution from salmon testes (Sigma-Aldrich).
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bioRxiv - Biophysics 2024Quote: ... 30% (mol) SM (Sigma-Aldrich), 10% (mol ...
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bioRxiv - Biophysics 2024Quote: ... 10% (mol) CHOL (Sigma-Aldrich), and 1% (mol ...
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bioRxiv - Biophysics 2024Quote: ... 0.5 mM β-mercaptoethanol) supplemented with Complete EDTA free protease inhibitor (Sigma). The cells were lysed using an microfluidizer (Microfluidics M-110L ...
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bioRxiv - Biophysics 2024Quote: ... Four different concentrations of sodium chloride (Sigma-Aldrich, ≥99.5 %, S9888) 0.2 ...
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bioRxiv - Biophysics 2024Quote: ... which was prepared with 20 mM HEPES-buffer (2-(4-(2-Hydroxyethyl)-1- piperazinyl)-ethansulfonsäure) (Sigma-Aldrich, ≥99.5 %, H3375) as calcium chloride is not soluble in DPBS-buffer ...
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bioRxiv - Biophysics 2024Quote: ... without calcium and magnesium (containing 8 mg/mL sodium) (Lot No. RNBJ1061, Sigma-Aldrich) for 45 minutes at room temperature with a magnetic stirrer at the lowest speed (100 rotations per minute ...
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bioRxiv - Biophysics 2024Quote: ... 0.1 mM ATP and 1 mM EGTA) supplemented with Complete EDTA free protease inhibitor (Sigma) and incubated on ice for 15 min ...
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bioRxiv - Biophysics 2024Quote: ... The lenses are 105mm fixed-focus lenses (Sigma, Japan). These cameras are strategically positioned above the observation chamber as illustrated in the Figure 1(a ...
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bioRxiv - Biophysics 2024Quote: ... blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO) solution for 1 h ...
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bioRxiv - Biophysics 2024Quote: Purified samples were exchanged into a structure preserving and MS compatible buffer surrogate ammonium acetate at 300 mM AmAc (99.99 % purity, Sigma-Aldrich), 1 mM DTT ...
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bioRxiv - Biophysics 2024Quote: ... a small drop of Halocarbon oil 700 (Sigma Aldrich, St. Louis, MI, US) was added on top of the embryos to prevent water evaporation while allowing oxygen permeation for necessary metabolism ...
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bioRxiv - Biophysics 2024Quote: ... Four different concentrations of desoxyribonucleic acid (DNA) single stranded from salmon testes (D9156, Sigma-Aldrich) 5 mg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Following 100 s of digestion at 10 °C with rapid mixing every 30 seconds, the sample was centrifuged (13,000 g, 15 s, 0 °C) through 0.22 µm PVDF filters (Millipore). The flow through was immediately snap frozen in liquid nitrogen for short-term storage ...
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bioRxiv - Biochemistry 2024Quote: ... A reference aliquot (t0) was removed before initiating degradation at 4 °C by addition of Proteinase K (Millipore) to a final concentration of 2.5 ng/µL ...
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bioRxiv - Biochemistry 2024Quote: ... 150nL of master mix (0.2% DDM (D4641-500MG, Sigma-Aldrich, USA/Germany), 100 mM TEAB (17902-500ML ...
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bioRxiv - Biochemistry 2024Quote: ... benzonase (Millipore) and Complete EDTA-free protease inhibitor cocktail (Roche) ...
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Cyborg islets: implanted flexible electronics reveal principles of human islet electrical maturationbioRxiv - Bioengineering 2024Quote: ... 24-hour metabolic shock / recovery cycles were applied as follows: cells were cultured in S3 medium containing 20 mM Glucose (Sigma; G7528) + 10 μM Forskolin (Stemgent ...
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bioRxiv - Bioengineering 2024Quote: ... Rabbit anti-Cx43 (1:400, Sigma, C6219) was used as a primary antibody with a goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or 633 (1:500 ...
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Cyborg islets: implanted flexible electronics reveal principles of human islet electrical maturationbioRxiv - Bioengineering 2024Quote: ... the single cells were suspended in DPBS (without Ca2+ and Mg2+) with 0.04% bovine serum albumin (Sigma) at a concentration of 1000 cells per microliter ...
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bioRxiv - Biochemistry 2024Quote: ... anti-FLAG-tag antibody (Sigma #F1804; 1,1000 dilution), anti-Pgk1 antiserum (a gift from J ...
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bioRxiv - Biochemistry 2024Quote: ... and 100 μg/mL herring testis DNA (Sigma) for 1-3 days ...
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bioRxiv - Biochemistry 2024Quote: ... the surface was incubated for 10 min with biotinylated BSA (Sigma-Aldrich, 3 mg/ml in PBS), washed several times with PBS ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µL of fully deuterated glycerol (glycerol-d8, Sigma-Aldrich) were added ...
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bioRxiv - Biochemistry 2024Quote: ... Thioflavin T and HEPES were purchased from Sigma Aldrich. Oxyma was purchased from Nova Biochem ...
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bioRxiv - Biochemistry 2024Quote: ... Subsequently unbound IAP was removed by diluting the samples three times 10-fold in labelling buffer and concentrating back to the original volume with an Amicon Ultra 100 kDa cutoff concentrator (Millipore). The protein concentrations for subsequent EPR experiments are given in Supplementary Table 1.
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bioRxiv - Biochemistry 2024Quote: ... and urea (ultragrade) were purchased from Sigma Aldrich. All salts and dialysis tubing were purchased from Fisher Scientific (Fair Lawn ...
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bioRxiv - Biochemistry 2024Quote: ... We used IAP (Sigma-Aldrich, cat. no. 253421) for labelling because it sustains highly basic pH values necessary for this study without the drawbacks of commonly used S-(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl methanesulfonothioate (MTSL)33 or maleimide-functionalized labels34 ...
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bioRxiv - Biochemistry 2024Quote: ... and dimethylated SOLu-trypsin were obtained from Sigma-Aldrich (Oakville, ON). Stable SpikeTides_L peptides were obtained from JPT Peptide Technologies (Germany) ...
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bioRxiv - Biochemistry 2024Quote: 1-2 μL from the reaction was spotted on a silica TLC plate (Millipore Sigma) and allowed to dry for a minimum of 15 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... and transferring onto a Polyvinylidene Fluoride (PVDF) membrane (Millipore, ISEQ00010). Membranes were subsequently incubated with the appropriate primary antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... by comparison to a standard stock solution of 100 µM of 4-Hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL, Sigma Aldrich) in water measured at the same conditions ...
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bioRxiv - Biochemistry 2024Quote: ... 1.5 μL from the reaction was spotted on a silica TLC plate (Millipore Sigma) and allowed to dry for a minimum of 15 minutes ...
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bioRxiv - Biophysics 2024Quote: ... and antibiotics - 100 µg/ml streptomycin with 100 units/ml penicillin (cat. No. 15140122 from Sigma-Aldrich, Darmstadt, Germany). The cells were grown as monolayers in an incubator under conditions of a humidified 5% CO2 atmosphere and 37 °C until they reached about 80% confluence ...
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bioRxiv - Biophysics 2024Quote: ... and the supernatant containing the solubilized protein was collected for affinity purification by batch-binding to 10 mL of M2-agarose FLAG resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 3 pmol of each primer (Sigma-Aldrich) and 2 μl of extracted DNA ...
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“Long-Term Quarantine is Associated with High Cortisol and Low DNA Methylation in New World monkeys”bioRxiv - Animal Behavior and Cognition 2024Quote: ... samples were incubated 1 ml methanol (Sigma-Aldrich, UK) in 15ml conical flasks and placed on a rotating surface for 24 hours ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... rabbit anti-Synapsin 1 (Millipore, AB1543, 1:1000), mouse anti-ubiquitin(Santa Cruz ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 1 hour at 37°C with PLUS and MINUS PLA probes (Sigma Aldrich). Then cells were incubated with the Ligase diluted in the ligation buffer for 30 minutes at 37 °C ...