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Citations for Millipore Sigma :
3251 - 3300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 0.5 g/L NaCl) supplemented with 1.7 g/L yeast nitrogen base without NH4Cl and amino acids (Sigma Y1251). 1 g/L 15NH4Cl and 4 g/L glucose were supplemented for 15N labelling ...
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bioRxiv - Biochemistry 2024Quote: ... MIB1 knockout was confirmed by TOPO cloning of the amplified genomic sequence and by the loss of MIB1 signal on western blot with antibodies for the N- and C-terminal portions of MIB1 (Abcam, ab124929; Millipore Sigma, M5948).
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bioRxiv - Biochemistry 2024Quote: ... Myosin sS1 (frozen or freshly prepared) was buffer exchanged into experimental buffer using 50 kDa MWCO Amicon filters (Cat # UFC5050BK, Millipore Sigma). Myosin sS1 and mant-ATP were rapidly mixed in 1 ...
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bioRxiv - Biochemistry 2024Quote: ... mouse anti-FLAG (Sigma F1804, 1:1000), HRP labelled goat anti-mouse IgG (Perkin Elmer ...
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bioRxiv - Biochemistry 2024Quote: ... and 100 U Turbonuclease from Serratia marcescens (Sigma). Samples were rotated for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was immunoprecipitated with Anti-FLAG M2 affinity Gel (Sigma). Samples were eluted off from the beads via 50 µL eluent (50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... Pre-adipocytes were cultured in cultured dishes on glass slides in DMEM-F12 medium (Thermo-12634010) with 10% FBS (Sigma-F7524), and supplemented with 2.5 µg/ml ßFGF (Sigma-F3685) ...
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bioRxiv - Biochemistry 2024Quote: ... S protein agarose (Millipore) was added to each sample ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 mM IBMX (Sigma-I5879), 5 µg/mL insuline (Sigma-I6634) ...
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bioRxiv - Biochemistry 2024Quote: ... and Rosetta(DE3)pLyS cells (Novagen, Merck Millipore) were transformed with plasmid of interest to produce proteins fused with an N-terminal hexa-histidine (His ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Biochemistry 2024Quote: ... N’’-triacetylchitotrioside (MUC, Sigma) (Langner ...
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bioRxiv - Biochemistry 2024Quote: ... 50 µL 0.5 mM benzamidine (Sigma Aldrich) and 400 µL of glass beads were added to each tube ...
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bioRxiv - Biochemistry 2024Quote: ... Gfp-tagged protein of interest was detected using a primary mouse anti-Gfp (1:3,000, Millipore/Sigma). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Biochemistry 2024Quote: Cells were harvested and resuspended in lysis buffer (40 mM HEPES pH 7.5, 10 mM NaCl, 1% Triton X-100, 1X protease inhibitor cocktail (Sigma P2714), 20 mM β-glycerophosphate ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µM dexamethason (Sigma-D4902) et 1 µM rosiglitazon (Sigma-R2408) ...
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bioRxiv - Biochemistry 2024Quote: ... 100 µL 0.1 M PMSF (Sigma/Aldrich), 100 µL Protease inhibitor cocktail (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... cell medium was supplemented with 100 µM oleic acid (Sigma-O1383) complexed with fatty acid-free BSA (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... and PI(4,5)P2 (L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine) ammonium salt) (catalog no. 840046P-1MG) were purchased from Sigma Aldrich/Merck (manufactured by Avanti Polar Lipids ...
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bioRxiv - Biochemistry 2024Quote: ... Cleaved His-tagged GB1 fragment and remaining TEV were removed via a second Ni-NTA purification after buffer exchange to buffer A using an Amicon Ultra-10K centrifugal filter (Merck Millipore). The tag-free protein was subjected to SEC using a Superdex 200 Increase 26/600 column equilibrated in HEPES buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... the induction was done either by 1% (w/v) lactose or 0.5 mM isopropyl-β-D-1-thio-galactopyranoside (IPTG, Sigma Aldrich) (Table 1) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µM cortisol (Sigma-H0369), 1 µM dexamethason (Sigma-D4902 ...
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bioRxiv - Biochemistry 2024Quote: ... and supplemented with 2.5 µg/ml ßFGF (Sigma-F3685). When cells reached confluence ...
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bioRxiv - Biochemistry 2024Quote: ... and 200 U Turbonuclease from Serratia marcescens (Sigma). Samples were rotated for 1 hour at 4°C before centrifuging at 9,000xg for 20 minutes at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5% NP-40 Alternative (Millipore 492016-500)) supplemented with 1 µg/mL aprotinin ...
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bioRxiv - Biochemistry 2024Quote: ... differentiation was initiated by the addition of an adipogenic cocktail containing 1 nM T3 (Sigma-T6397), 0.5 mM IBMX (Sigma-I5879) ...
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bioRxiv - Biochemistry 2024Quote: ... complexed with fatty acid-free BSA (Sigma) for 2.5 hours ...
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bioRxiv - Biochemistry 2024Quote: ... a primary anti-GST (Sigma, 1:3,000) was used to detect bound PI(4,5)P2-grip protein.
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bioRxiv - Biochemistry 2024Quote: ... pyridoxal 5’-phosphate monohydrate and TLC Silica gel 60 F254 (20 cm x 20 cm) were purchased from Sigma-Aldrich. Sodium L-glutamate monohydrate ...
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bioRxiv - Biochemistry 2024Quote: ... et 1 µM rosiglitazon (Sigma-R2408). After day 6 ...
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bioRxiv - Biochemistry 2024Quote: ... fixed with 0.05% glutaraldehyde (Sigma, #G6257) in PBS at room temperature for 10 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... Oxidation was quenched by addition of 300 mM sodium sulfide (Sigma). Peptides were desalted using homemade C18 columns and eluted with 0.1% formic acid in 50/50 formic acid/acetonitrile to a final concentration of 15 µg/mL.
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bioRxiv - Biochemistry 2024Quote: ... 0.4mg of MTT (M5655; Sigma-Aldrich) was added to each well of a 96-well plate ...
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bioRxiv - Biochemistry 2024Quote: ... and Rosetta(DE3)pLyS cells (Novagen, Merck Millipore) were transformed with plasmid of interest to produce proteins fused with an N-terminal hexa-histidine (His ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µg/mL insuline (Sigma-I6634), 1 µM cortisol (Sigma-H0369) ...
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bioRxiv - Biochemistry 2024Quote: ... Immobilon-PSQ polyvinylidene fluoride (PVDF) membrane (0.2 µm; ISEQ00010, Millipore, USA) was used as a protein carrier in this step ...
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bioRxiv - Biochemistry 2024Quote: ... or 50 µM 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) (Sigma Aldrich, Darmstadt, Germany) respectively for 1 h ...
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bioRxiv - Biochemistry 2024Quote: ... 5–15 µU glutathione reductase (G3664-100UN, Sigma Aldrich, St. Louis, MO, United States)) and 1.07 ng.µL-1 isocitrate dehydrogenase (I2411-10UG ...
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bioRxiv - Biochemistry 2024Quote: ... 2.5 mL 70% H2SO4 and 0.5% (v/v) Antifoam 204 (Sigma Aldrich, St. Louis, MO, United States). Fermentor runs were initiated by the addition of a 20 ml starter culture grown at 30°C to stationary phase in HC medium lacking histidine for plasmid retention ...
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bioRxiv - Biochemistry 2024Quote: ... cells were subsequently treated with 1 µM auranofin (Sigma Aldrich, Darmstadt, Germany) or 50 µM 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU ...
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bioRxiv - Biochemistry 2024Quote: ... 0.2 M lithium acetate (Sigma Aldrich, St. Louis, MO, United States) and 0.1 M dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2024Quote: ... the mice were anesthetized with sodium pentobarbital (injection dose 50 mg/kg; Sigma). The blood taken from the abdominal aorta was used to separate serum which was applied to detect the insulin (μg/L ...
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bioRxiv - Biochemistry 2024Quote: ... DL Dithiothreitol (Sigma Aldrich) and ExcelBand™ 3-color Regular Range Protein Marker (SMOBIO ...
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bioRxiv - Biochemistry 2024Quote: ... the amplified genes were cloned into the pET28b(+) plasmid (Novagen). Notably ...
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bioRxiv - Bioengineering 2024Quote: ... The surface of the dried extract was covered with 1 µL of a matrix solution: a 20mg/ml solution of 2,5-dihydroxybenzoic acid (Sigma Aldrich, United States) in 30% acetonitrile with 0.5% trifluoroacetic acid (Panreac ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR product was then mixed with 1/3 volume 10 M ammonium acetate (Sigma, A1542) and 2 volume ethanol (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... The PicoProbeTM assay (Sigma Millipore) and analysis were performed according to the manufacturer’s protocols using the picomole standard curve (n = 1) ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of 1 M perchloric acid (Sigma Millipore) was added to resuspended cells and sonicated at 10% power for three one-second cycles with three-second rests between cycles ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of 1 M perchloric acid (Sigma Millipore) was added to resuspended cells and sonicated at 10% power for three one-second cycles with three-second rests between cycles ...