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Citations for Millipore Sigma :
3251 - 3300 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... A centrifuged cell pellet with 3 million cells in total was mixed with 54 µL of cold GM (32.5% v/v) supplemented with 1 mg/mL of ACA (Sigma-Aldrich), 12 µL of a stock solution of 50 U/mL of thrombin (7.5% v/v) ...
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bioRxiv - Cell Biology 2021Quote: ... myoblasts were differentiated into myotubes in DMEM supplemented with 2% (v/v) of horse serum (Sigma-Aldrich, Cambridgeshire, UK, H1270), 1% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Gels were incubated with Coomassie stain (60mg/l Coomassie Blue R-250, 10% v/v acetic acid, both Sigma, UK) Following 24h de-staining in 10% v/v acetic acid ...
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bioRxiv - Plant Biology 2021Quote: ... supernatants were discarded and the beads were washed 5 times using extraction buffer containing 0.5 % (v/v) IGEPAL CA-630 (Sigma-Aldrich). To release the proteins ...
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bioRxiv - Plant Biology 2020Quote: ... The membranes were blocked with 5% (w/v) skim milk in a phosphate-buffered saline (PBS) solution plus 0.1% (w/v) Tween 20 (Sigma-Aldrich). The target proteins were immunodecorated with primary antibodies and then incubated with horseradish peroxidase-conjugated anti-rabbit IgG antibodies (Catalogue number ...
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bioRxiv - Neuroscience 2019Quote: ... Postfixed for 2 h with 1% (w/v) osmium tetroxide (TAAB Lab) containing 0.8% (w/v) potassium hexacyanoferrate (Sigma-Aldrich) in PB ...
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bioRxiv - Cell Biology 2021Quote: ... the rest pieces were digested by shaking in 2 mL of 5% (v/v) FBS in RPMI medium containing DNase I (Sigma) (150 μg/ml ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was quenched by adding 5% (v/v) trichloroacetic acid (Sigma Aldrich, catalog #T0699, CAS-no:76-03-9). BPA concentration was analyzed by LC-MS as described below.
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bioRxiv - Microbiology 2021Quote: ... They were then fixed with 4% v/v PFA and plaques were revealed by staining with a 0.1% crystal violet solution (V5265, Sigma-Aldrich). For the washout experiments ...
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bioRxiv - Microbiology 2021Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
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bioRxiv - Plant Biology 2020Quote: ... flowers from stages 7–9 were selected from the inflorescences and soaked in 4% (w/v) paraformaldehyde and 2.5% (w/v) glutaraldehyde (Sigma, G5882). After vacuum infiltration for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... All samples were then boiled at 95 °C for 10 mins at a 1:1 (v/v) ratio in 2x Laemmli loading dye (Sigma) before being electrophoresed through a 4-12% NuPAGE Bis-Tris polyacrylamide gel (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... in blocking buffer (supernatant of 5% w/v milk powder in PBS centrifuged at maximum speed for 10 min) followed by staining with secondary antibody which was diluted in 10% v/v normal goat serum (Sigma) in blocking buffer and incubated for 2 hours at room temperature in a humid chamber ...
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bioRxiv - Molecular Biology 2021Quote: D7+17 organoids were exposed to 10% (v/v) plasmapheresis plasma supplemented with anticoagulants heparin (100 μg/ml, Sigma Aldrich) and PPACK (10 μM ...
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bioRxiv - Plant Biology 2021Quote: ... Seeds were surface-sterilized and germinated on 1/2 Murashige and Skoog (MS) medium (Duchefa Biochimie) supplemented with sucrose (1% w/v, Duchefa Biochimie) and Select Agar (0.8% w/v, Sigma-Aldrich) and stratified in the dark at 4°C for 48h ...
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bioRxiv - Cell Biology 2021Quote: ... The reacted mixture was dialyzed in PBST buffer (DPBS with 0.05% v/v Tween 20) by a centrifugal filter with a 3 KDa molecular weight cut-off (Millipore UFC500324). The supernatant was immunoprecipitated by custom-made anti-RFP-beads (see Immunoprecipitation [IP] and Co-IP below) ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were washed in PB five times and post-fixed in 1% osmium tetroxide (Agar Scientific, R1023, 4% solution osmium tetroxide) and 1.5% potassium ferrocyanide (v/v) (SIGMA ALDRICH, P3289-100G ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.2 and immediately fixed with 2.5 % (v/v) glutaraldehyde (Science Services GmbH, Munich, Germany) in 75 mM cacodylate (Sigma-Aldrich), 75 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... cells were washed in 60% isopropanol and stained in an ORO solution (2:3 v/v H2O: isopropanol, containing 0.5% ORO, Sigma O0625) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Free-floating sections were rinsed with phosphate-buffered saline (PBS) and endogenous peroxidase activity was blocked by incubation in 0.3% v/v hydrogen peroxide (Sigma, UK) in PBS for 15 min ...
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bioRxiv - Bioengineering 2022Quote: ... 0.5 mmol AuCOOH NPs were added to 200 mL ethanol (80% v/v) containing 1.44 g 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Sigma-Aldrich) and 0.65 g N-hydroxysulfosuccinimide (NHS ...
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bioRxiv - Microbiology 2022Quote: ... They were then fixed with 4% v/v paraformaldehyde (PFA) and plaques were revealed by staining with a 0.1% crystal violet solution (V5265, Sigma-Aldrich).
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bioRxiv - Plant Biology 2022Quote: ... the tissue was ground to a fine powder with liquid nitrogen and then resuspended in a lysis buffer (50 mM Tris-HCl, pH 7.5; 0.1% [v/v] IGEPAL CA-630 [Sigma-Aldrich]; 2 mM phenylmethylsulfonyl fluoride [PMSF; Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% (v/v) antibiotic-antimycotic solution (25 µg/ml amphotericin B, 10 mg/ml streptomycin, 10,000 units of penicillin (Sigma-Aldrich)) (complete DMEM ...
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bioRxiv - Microbiology 2020Quote: ... The culture was supplemented with 2 ml (v/v) sterile amberlite resin XAD-16 (Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany). After 60 hours of incubation ...
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bioRxiv - Microbiology 2021Quote: ... and then fixed in 4% formaldehyde solution (v/v) for 20 minutes at room temperature followed by neutralization with 50 mM NH4Cl (Sigma) for 3 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed once with PBST and then incubated with the appropriate primary antibody diluted in PBST + 3% (v/v) BSA (Sigma) for 2 hours at RT ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... After 4 days cells were fixed and stained by adding 50 µl formaldehyde (15% w/v) and crystal violet (0.1% w/v) (Sigma-Aldrich) overnight ...
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bioRxiv - Cell Biology 2019Quote: ... #4 inguinal mammary glands were stained for several hours in carmine-alumn staining solution (2% w/v Carmine, Sigma; 5 % w/v Aluminium Potassium Sulfate, Sigma). After the desired color had developed ...
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bioRxiv - Genetics 2021Quote: ... NMDA receptor subunit 2 C-terminal peptide “SIESDV” was synthesised (Pepceuticals) and fully dissolved in 90% v/v methanol + 1M HEPES pH7 (both Sigma). Dissolved peptide was coupled to Affi-Gel 10 resin (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... with freshly added phosphatase inhibitors (50 mM NaF and 1 mM Na3VO4) and 1% (v/v) mammalian protease inhibitor cocktail (Sigma). Sonication was conducted at 50% output (Q55 Sonicator ...
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bioRxiv - Cancer Biology 2020Quote: ... flow channels of the Drop-seq chip were subjected to a hydrophobicity treatment using 1H,1H,2H,2H-Perfluorodecyltrichlorosilane (in 2% v/v in FC-40 oil; Alfa Aeser/Sigma). After 5 min of treatment ...
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bioRxiv - Microbiology 2020Quote: ... The plasma-treated coverslips were then transferred into 150 mL of 1% (v/v) (3-Aminopropyl)triethoxysilane (APTES) (Sigma-Aldrich) in toluene (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were maintained in growth medium: optimized DMEM from Addex Bio (catalog number C0003-02) supplemented with 20% (v/v) FBS (Sigma), 1× Penicillin/Streptomycin (Corning) ...
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bioRxiv - Immunology 2021Quote: ... Germany) were precoated with murine anti-HIS mAb at a dilution of 1:2,500 (v/v; Sigma-Aldrich, Munich, Germany) at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... brain slices were incubated overnight in a solution of PBS 0.01 mM/Triton™ X100 0.3% (v/v; CAT#T9284, Sigma-Aldrich) containing streptavidine-488 ...
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bioRxiv - Neuroscience 2020Quote: ... the slices were incubated overnight in a solution of PBS 0.01 mM/Triton™ X100 0.3% (v/v; CAT#T9284, Sigma-Aldrich) containing streptavidine-CY3 Zymax (1/1000 ...
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bioRxiv - Microbiology 2022Quote: ... monolayers were washed three times with PBS to remove unbound extracellular bacteria and then lysed with PBS containing 0.1% (v/v) Triton X-100 (Sigma-Aldrich) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... PEG 3350 40% v/v) with the DNA mix to be recombined and 50 μg of salmon sperm denaturated DNA (31149, Sigma) and then cooled for at least 5 min on ice ...
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bioRxiv - Biophysics 2019Quote: ... Cells were passaged twice a week and differentiated into a neutrophil-like phenotype by adding 1.25 % (v/v) of dimethyl sulfoxide (DMSO, Sigma-Aldrich) to the cell suspension the first day after passage and ...
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bioRxiv - Microbiology 2019Quote: ... Transformants were selected in MA medium plates supplemented with 1% (w/v) glucose and 2% (w/v) agar (Sigma-Aldrich). Selection was carried out in the absence of uridine ...
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bioRxiv - Biophysics 2019Quote: ... The slab was then immediately immersed in a 5% (v/v) solution of APTES in HPLC-grade ethanol (Sigma, 459828) for 20 min ...
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bioRxiv - Immunology 2019Quote: ... membranes were incubated with the indicated primary antibody in either PBST containing 5% skim milk or 5% (w/v) BSA (Fraction V, Sigma) for at least 1h at RT or overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... 0.25 x 106 total lymph node cells were cultured in 250 ul of RPMI 1640 media (Cellgro) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS, Sigma), 50 μM 2-mercaptoethanol ...
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bioRxiv - Bioengineering 2021Quote: ... cells were fixed with 3.6% (v/v) PFA for 15 min at RT in PBS and pellet sections stained with Alcian blue (Sigma-Aldrich). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.05% (v/v) Tween-20 in PBS) and incubated with primary antibody anti-LIG3 (1:150, Sigma-Aldrich, #HPA006723) in staining buffer for 2hr at RT ...
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bioRxiv - Plant Biology 2021Quote: ... 10%glycerol, 1 mM DTT, 1 mM EDTA, 0.5% Triton X-100 and 1% [v/v] protease inhibitor cocktail; Sigma-Aldrich). Insoluble debris was pelleted by centrifugation for 20 min with 4000 rpm at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... 5 mM EDTA, 1 mM NaF, 10 mM DTT, 0.1% Triton X-100 and 1% [v/v] protease inhibitor cocktail; Sigma-Aldrich). Protein extracts were boiled at 95°C for 10 min in 1x sodium-dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was washed briefly in Buffer A before being resuspended by vortexing in 150 μl of Buffer B (150 mM NaCl, 50 mM pH 7.4, 1M Hexylene glycol, 1% v/v Igepal (CA-630, Sigma, UK) supplemented with protease inhibitors ...
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bioRxiv - Microbiology 2020Quote: ... 6 h post transfection cells were fixed in 4% PFA and permeabilised with 0.1% (v/v) Triton X-100 (Sigma-Aldrich)in PBS for 15 min ...