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Citations for Millipore Sigma :
3151 - 3200 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Cultures (50 mL) were subjected to centrifugation in the presence of 0.01% (v/v) pluronic acid (Sigma-Aldrich, Germany) at 4°C ...
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Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... nonionic surfactant and 500 μL Tris 7.0 buffer containing 0.1% (v/v) Tween-20 (Sigma, catalog no. P1379-25ML) three times respectively ...
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bioRxiv - Plant Biology 2022Quote: ... medium (Murashige and Skoog basal salt mixture [PhytoTechnologies Laboratories], pH of 5.7, containing 0.8% [w/v] agar [Difco] and 1% [w/v] sucrose [Sigma-Aldrich]). Unless indicated otherwise ...
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bioRxiv - Synthetic Biology 2022Quote: ... The silanization solution was freshly prepared by mixing MilliQ water with 0.4% (v/v) 3-(Trimethoxysilyl)propylmethacrylat (TMSPMA, Sigma) and 0.4% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: HeLa cells were maintained using Dulbecco’s modified Eagle’s medium (DMEM) containing 10 % (v/v) fetal bovine serum (Sigma-Aldrich), and 1 % Glutamax (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... the protein was extracted from 1 g leaves using 2 mL extraction buffer (no. P0013; Beyotime) with 0.1 mM PMSF (no. ST507; Beyotime) and 0.1% (v/v) protease inhibitor cocktail (Sigma-Aldrich). The protein sample was centrifuged at 14000 g for 10 min at 4°C and then the supernatant was transferred in a new Eppendorf tube to incubate with 20 μl GFP-Trap-M beads (Chromotek ...
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bioRxiv - Neuroscience 2023Quote: Cells were fixed in cold 4% paraformaldehyde (Australian Biostain) on ice and permeabilized using 0.2% (v/v) Triton X-100 (Sigma) in PBS (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... 0.05 % w/v deoxyribonucleic acid from herring sperm and 0.15 % w/v NH4NO3 adjusted to pH 7.0 with NaOH (Sigma Aldrich) and supplemented with 1% glucose ...
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bioRxiv - Plant Biology 2023Quote: ... above the tips with the help of forceps and placed into a 35-mm-diameter dish containing a 70 µm strainer and 3 ml enzyme solution (1.25% [w/v] Cellulase [“ONOZUKA” R10, Yakult], 0.1% [w/v] Pectolyase [P-3026, Sigma-Aldrich] ...
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bioRxiv - Plant Biology 2023Quote: ... The pellet was resuspended in 1 ml NIBA buffer containing 1:100 (v/v) protease inhibitor cocktail (Sigma, P9599). Triton X-100 (1% final concentration ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were cultured in Dulbecco’s modified Eagle’s medium (Nacalai Tesque) supplemented with 10% (v/v) fetal calf serum (Sigma-Aldrich) and maintained at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Developmental Biology 2022Quote: ... BP310-1], 10 mM NaCl [Fisher, Cat. M-11624], 3 mM MgCl2 [Sigma, Cat. M8226], 0.1% IGEPAL [v/v; Sigma, I8896] ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellet was re-extracted with 450 µL methanol:water (2:1, v/v) containing 1nmol of scyllo-inositol (Sigma) as an internal standard ...
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bioRxiv - Biochemistry 2023Quote: ... Total cell lysates were prepared by collecting cells in 250 μl of ice-cold lysis buffer (50 mM Tris pH7.5, 150 mM NaCl, 1 mM EDTA, 1% (v/v) IGEPAL CA-630 (Sigma), 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.1%(v/v) Triton X-100] with freshly added 30 µl of protease inhibitor cocktail (PIC, Sigma-Aldrich), 3 µl of 100 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Biophysics 2024Quote: ... in MTSB for 10 min at room temperature or directly fixed using 4%(v/v) formaldehyde (Sigma-Aldrich, Germany) and 0.1%(v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Lipids were resuspended in chloroform: methanol (2:1 v/v) and loaded onto a silica TLC plate (Sigma 60778). For nonpolar lipid separation ...
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bioRxiv - Biophysics 2024Quote: ... the u-Flux chamber was then sequentially flushed with 0.1% (w/v) bovine serum albumin (BSA) and 0.5% (w/v) Pluronic acid F-127 (Millipore Sigma). All flushes above were performed at 2 bars of pressure for at least 10 min under the control of the u-Flux microfluidics pumping system (LUMICKS ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell pellets were resuspended in staining wash buffer (0.5% (w/v) BSA and 0.1% (w/v) sodium azide (S2002; Sigma Aldrich) in DPBS ...
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bioRxiv - Bioengineering 2023Quote: ... was weighted and placed into a tube containing 2 mL 0.2% v/v Triton X-100 (108603, Sigma-Aldrich) and 5 mM MgCl2 (M2393 ...
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bioRxiv - Molecular Biology 2023Quote: ... probing was performed by splitting the sample into 25 μl aliquots and mixing with 2.78 μl of 6.5% (v/v) DMS (Sigma-Aldrich) in anhydrous ethanol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 1 3 v/v) at 80°C for 2 h after addition of 50 nmol 5α-Cholestane (Sigma, C8003) as internal standard for neutral sterol analysis ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: Water-soluble metabolites were extracted with 0.5 ml ice-cold MeOH/H2O (80/20, v/v) containing 0.01 μM lamivudine (Sigma-Aldrich) and 0.5 μM sucrose (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were incubated with primary antibodies overnight at 4 °C in 2.5 % dry milk and washed with PBS-T (PBS containing 0.1 % v/v Tween 20, Sigma-Aldrich) before incubation with anti-mouse (#7076S ...
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bioRxiv - Molecular Biology 2023Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto 0.45 µm Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
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bioRxiv - Immunology 2023Quote: ... in DPBS for 1 h at 37°C and blocked with 1.5% (v/v) bovine serum albumin (Sigma-Aldrich) in DPBS overnight at 4°C.
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bioRxiv - Biochemistry 2023Quote: ... was grown in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% v/v fetal bovine serum (FBS) (Sigma Aldrich), 0.24 mg/mL streptomycin sulphate (BioChemica) ...
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bioRxiv - Physiology 2023Quote: ... 100 µM C16-alkyne was conjugated with fatty acid-free BSA (5% v/v) (Sigma-Aldrich, catalog no. A7030) in DMEM LG or HG media by sonication (15 min ...
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bioRxiv - Bioengineering 2023Quote: ... the unencapsulated mRNA content (Cf) and the mRNA content (Ct) of the same sample after adding 0.1% (v/v) Triton X-100 (Sigma) were measured to calculate the mRNA encapsulation efficiency ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with primary antibodies diluted in TBS-T (20 mM Tris pH 7.5, 150 mM NaCl supplemented with 0.2% (v/v) Tween-20 (Sigma Aldrich)) containing 5% non-fat milk buffer (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were anaesthetized first with Halothane (2-Bromo-2-Chloro-1,1,1-trifluoroethane, Sigma-Aldrich, Germany; 1/1000 v/v), followed by a euthanasic dose of Dolethal (100 mg/kg of sodium pentobarbital ...
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bioRxiv - Microbiology 2023Quote: ... hansenii NQ5 were picked into 5mL HS media with 0.2% (v/v) cellulase from Trichoderma reesei (Sigma-Aldrich C2730) and grown to stationary phase ...
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bioRxiv - Immunology 2023Quote: ... for 15 min at room temperature and blocked with 5% v/v goat serum in permeabilisation buffer (0.5% w/v bovine serum albumin and 0.1% w/v Saponin in PBS; Sigma-Aldrich). Permeabilisation buffer was used to dilute all antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... weighted and incubated in 2 mL lysis solution consisting of 1% v/v Triton-X-100 (108603, Sigma-Aldrich) + 5 mM MgCl2 (M2393 ...
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bioRxiv - Biochemistry 2022Quote: ... Colonies were selected, cultured in Middlebrook 7H9 (Difco, cat.#271310) containing 0.05% (v/v) Tween 80 (Sigma, cat. #P1754) and appropriate antibiotics ...
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bioRxiv - Biochemistry 2022Quote: ... They were stained with 1/400 (v/v) of primary antibody mouse anti rhodopsin clone 4D2 (Sigma-Aldrich: MABN15) and allowed to incubate for 30 minutes then washed with PBS containing 15 mM NaN3 and 2% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow cells were cultured in Iscove’s Modified Dulbecco’s media supplemented with 10% (v/v) FBS (Cat: 12103C, Millipore Sigma), 100 unit/ml penicillin-streptomycin (Cat ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5 % [v/v] Triton-X-100) and incubated with anti-HA or anti-FLAG M2 resin (Millipore-Sigma, USA) for 1 h at 4°C for immunoprecipitation of protected fragments.
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bioRxiv - Immunology 2023Quote: ... 100 μg of peptide was prepared in a 50 μL emulsification of 1:1 (v/v) normal saline and complete Freund’s adjuvant (CFA)(Sigma) and injected subcutaneously at the base of the tail ...
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bioRxiv - Biochemistry 2023Quote: ... and gently resuspended in cold fractionation buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 0.5% v/v IGEPAL® CA-630 (Sigma)) supplemented with Pierce™ Protease Inhibitor Mini Tablet (ThermoFisher A32955 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5 % [v/v] Triton-X-100) and incubated with anti-HA or anti-FLAG M2 resin (Millipore-Sigma, USA) for 1 h at 4°C for immunoprecipitation of protected fragments.
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 10 min and cells were permeabilised in 0.5% Triton X-100 (v/v, Cat.# T8787, Sigma) in PBS for 20 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and resuspended in 500 μL PI/Triton X-100 staining solution [0.1% (v/v) Triton X-100 (Sigma-Aldrich) in PBS add 0.1 mg DNAse-free RNAse A (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... Secondary antibody incubation was performed for 30min with 1:10000 (v/v) anti-rabbit IgG-peroxidase antibody (Sigma-Aldrich). Again ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were plated on gelatin-coated (0.1% v/v) Nunc 10-cm dishes in serum-free ESGRO Complete Clonal Grade Medium (Millipore) to which was added 1,000 U/ml LIF ...
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bioRxiv - Biochemistry 2023Quote: ... 0.6 % (v/v) n-dodecyl-β-D-maltoside (DDM from Inalco Pharmaceuticals) and 1.6 mg/mL iodoacetamide (Sigma-Aldrich) for 20 min at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... PFA (Alpha Aesar) for 30 min and permeabilized with 0.2 to 0.5% (v/v) Triton X-100 (Sigma-Aldrich) for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed by centrifugation in PBS and resuspended in 5% (v/v) bovine calf serum (Sigma-Aldrich, F7524) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... They were stained with 1/400 (v/v) of primary antibody mouse anti-rhodopsin clone 4D2 (MABN15, Sigma-Aldrich) and allowed to incubate for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... 3 w/v% PEG-PQ-PEG and 3.5 mM PEG-RGDS were dissolved in HEPES-buffered saline (HBS, pH 8.3) with 1.5% (v/v) triethanolamine (TEOA, Sigma-Aldrich), 0.01 mM eosin Y ...