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Citations for Millipore Sigma :
3301 - 3350 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Coverslips were washed in PBS and blocked in blocking buffer (3% (w/v) BSA in PBS) and the primary antibody applied at the relevant dilution in 1% (w/v) BSA (Sigma) in PBS and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Reagents including heavy isotopes were purchased from Sigma 5% 2H2O supplemented solutions were made up v/v with 99.9% deuterium oxide while 15N solutions contained K15NO3 98% and 15NH415NO3 98% obtained from Sigma Aldrich.
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bioRxiv - Molecular Biology 2020Quote: ... The sample was crosslinked to increase the amount of complex formed by addition of 0.0125% (v/v) glutaraldehyde (Sigma-Aldrich), at room temperature for 15 min before quenching with 200 mM Tris pH 7.4 (final concentration) ...
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bioRxiv - Cell Biology 2019Quote: ... 0.5% (v/v) sarkosyl) supplemented with 5 μg/ml protease inhibitor cocktail and 1 mM phenylmethylsulfonyl fluoride (PMSF; Sigma-Aldrich). Nuclear protein isolation was performed with the NE-PER™ kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Cy5- or Cy7-PMPC25-PDPA68 copolymer was dissolved together with the PMPC25-PDPA68 in an organic solution of 3:1 (v/v) methanol:tetrahydrofuran (Sigma-Aldrich) for a final copolymer concentration of 20 mg/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... All single-cell samples were distributed at 0.5×106 cells/mL for live staining with monoclonal mouse anti-human FOLR1 IgG1 (LS Bio) in DPBS with 2% v/v FBS (Sigma). Secondary staining for target was performed using QIFIKIT® (BIOCYTEX ...
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bioRxiv - Microbiology 2021Quote: ... stock solutions of the yeasts were elaborated at a final concentration of 2.5% (v/v) in a mixture of Tween 80 and ethanol (1:1) (Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: ... programed for uniform mixing as previously described at a controlled temperature and modified with online automated desalting.13 Proteins were denatured in a solution of 35% v/v 2-2-2 Trifluoroethanol (TFE, Sigma), 40 mM Dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2020Quote: ... round #1 German glass coverslips (Electron Microscopy Services) were coated with 0.5% v/v (3-Aminopropyl)triethoxysilane (APTES, Sigma, 440140), 99.2% v/v ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... After blocking the membrane with 2 % non-fat dry milk dissolved in PBS + 0.1 % (v/v) tween-20 (PBST; Sigma-Aldrich), membranes were incubated with primary antibodies for either 2 hours at room temperature or overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The nucleic acid pellet was washed twice with ethanol and eluted in 100 μL water containing 10 μM Tris-HCl and 0.05% Triton X-100 (v/v; Sigma-Aldrich). DNA quantity and purity were assessed using a NanoDrop 2000 (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Primary lung fibroblasts were isolated from disaggregated tissue samples as described previously14 and cultured in DMEM supplemented with L-glutamine (1% v/v; Sigma), penicillin-streptomycin (1% v/v ...
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bioRxiv - Cell Biology 2021Quote: ... HA-tagged or direct knockout parasites lines from single T25 flasks were harvested and lysed in 50 μl lysis buffer (1% v/v Triton-X 100, 1 mM MgCl2, 1X cOmplete EDTA-free protease inhibitor cocktail (Sigma), 0.1 % v/v Benzonase (Merck) ...
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bioRxiv - Microbiology 2020Quote: ... 3 µg of RNA were separated on MEN-buffered 1.5% agarose gels that provided denaturing conditions by 6% formaldehyde using an RNA sample loading buffer containing a final concentration of 62.5% (v/v) deionized formamide (Sigma-Aldrich). Afterwards ...
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bioRxiv - Microbiology 2021Quote: ... These ranged from ranged from high to medium affinity and a variety of coating methods were assessed: 1/ 100 μL 0.025 % (v/v) glutaraldehyde (Sigma, UK) which was incubated at 37 °C with shaking for 1h ...
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bioRxiv - Microbiology 2020Quote: Human HeLa229 cells (ATCC CCL-2.1tm) and human epithelial conjunctival cells (Chang) were cultured in 10 % (v/v) heat inactivated FBS (Sigma-Aldrich) RPMI1640 + GlutaMAXtm medium (Gibcotm ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of each culture supernatant were mixed with 50 μL of Griess reagent [1:1 (v/v) 0,1% N-(1-naphthyl) ethylenediamine dihydrochloride (NEED, Sigma-Aldrich) in water ...
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bioRxiv - Immunology 2020Quote: ... The protein was eluted with PBS containing 300 mM imidazole and 0.1% (v/v) protease inhibitor cocktail (Sigma, P-8849). For the CR3022 antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... and grown in the HyClone SFM4 Insect cell culture (SFM4) medium (GE Lifesciences SH30913.02) with 1% (v/v) Streptomycin-Penicillin (Sigma P4333) following standard procedures (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... transferred to individual 15 ml conical tubes with 10x volumes of RPMI with 10% (v/v) heat-inactivated FBS (Sigma) followed by centrifugation at 300 x g for 7 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... washed in ice-cold PBS and lysed in room temperature NP40 lysis buffer (50 mM Tris pH 7.4, 140 mM NaCl, 3 Na3VO4, 1% v/v IGEPAL CA-360 (Sigma, #I8896), Phosphatase Inhibitor cocktail (PhosStop ...
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bioRxiv - Biochemistry 2022Quote: ... 0.05% [w/v] digitonin [Sigma: D141], 10% [w/v] glycerol, and 0.4 mg/mL, and 1x FLAG-peptide [Sigma: F3290]) (30 min ...
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bioRxiv - Bioengineering 2022Quote: ... according to methods previously published.[28,29] Microcapsules were constituted of 1.0% w/v alginic acid (PH163S2, JRS Pharma GmbH, Germany) and 1.0% w/v gelatin type A (Sigma-Aldrich, Germany). These polymers were dissolved in a solution containing 0.1M HEPES (pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed once with PBST and then incubated with the appropriate primary antibody diluted in PBST + 3% (v/v) BSA (Sigma) for 2 hours at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... round #1 German glass coverslips (Electron Microscopy Services) were coated with 0.5% v/v (3-Aminopropyl)triethoxysilane (APTES, Sigma, 440140), 99.2% v/v ethanol ...
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bioRxiv - Microbiology 2022Quote: ... then cells were replenished with DMEM containing 10% (v/v) FCS and 50 μg/ml gentamicin (Sigma Aldrich, cat# G1397) and incubated for 72 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: Culture aliquots (200 μl) sampled for flow cytometry were fixed using 0.25% (v/v) glutaraldehyde (grade II, Sigma Aldrich, USA) and stored at −80°C until analysis (Marie et al. ...
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bioRxiv - Plant Biology 2022Quote: ... The density of nematodes was adjusted to 1 nematode/µL using sterile double distilled H2O containing 0.01% v/v Tween (Sigma-Aldrich). Once the plants reached an age of 21-28 days ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with primary antibody diluted in PBS supplemented with 5% Donkey Serum (v/v) and 0.1% Triton-X-100 (Sigma, T8787). Primary antibodies included anti-O1 (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... cell monolayers were rinsed with ice-cold PBS and then lysed on the dish with 1 mL of lysis buffer (0.1% [v/v] IGEPAL CA-630 in PBS with protease inhibitor cocktail [Sigma-Aldrich]) for 20 minutes on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... sections were fixed with ice-cold 100 % acetone for 10 min at −20 °C and washed with PBS-T (0.1 % v/v Tween 20, Sigma-Aldrich) before blocking with 5 % BSA in PBS (m/v ...
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bioRxiv - Plant Biology 2023Quote: ... Four grams of seedling tissue from glk1 glk2 and 35S:GLK2 plants were harvested and crosslinked with 1% (v/v) formaldehyde (Sigma-Aldrich) for 20 min under a vacuum ...
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Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cellsbioRxiv - Neuroscience 2022Quote: ... Cochlear cultures were treated for 6 h with various reagents (all dissolved in culture medium): 0.1% (v/v) DMSO (276855; Sigma-Aldrich), 0.5 mM BAPTA-AM (A1076 ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was resuspended in lysis buffer (50 mM Tris pH 8, 1 M NaCl, 10% v/v BugBuster (Novagen), 10 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... 5% v/v glycerol and 1 mM TCEP and then concentrated using 10 kDa MWCO Amicon Centrifugal Filters (Millipore Sigma) according to manufacturer’s directions ...
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bioRxiv - Biophysics 2024Quote: ... Antibodies were eluted at 0.8 mg/mL in 0.1 M glycine buffer at pH 2.8 and immediately neutralized with 1/10 v/v 1M Trizma hydrochloride solution (Sigma Aldrich) at pH 9.0 to obtain the final pH at 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... the following modifications were made: cells were instead fixed using 2% [w/v] PFA + 1.5% [w/v] glutaraldehyde (GA, Sigma 5882) at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: Cold PBS was used to wash the cells and RIPA buffer supplemented with 2% (v/v) protease inhibitor cocktail (Sigma) and 1% phosphatase inhibitor cocktail (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM DTT and 5%(v/v) glycerol by five cycles of filtration through 10 kDa Amicon filters (Merck Millipore), and finally concentrated to 0.5 mg/ml.
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bioRxiv - Microbiology 2023Quote: ... They were then fixed with 4% v/v PFA and plaques were revealed by staining with a 0.1% crystal violet solution (V5265, Sigma-Aldrich). Pictures of the plaques were acquired with an Olympus MVX10 microscope and plaques area were measured using the ZEN software v2.5 (Zeiss) ...
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bioRxiv - Plant Biology 2023Quote: ... then germinated on 1/2 Murashige and Skoog (MS) medium (Duchefa Biochimie) supplemented with sucrose (1% w/v, Duchefa Biochimie) and Select Agar (0.8% w/v, Sigma-Aldrich) after stratification in the dark at 4°C for 48h ...
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bioRxiv - Cell Biology 2023Quote: ... were routinely cultured in keratinocyte growth medium (KGM) (Allen-Hoffmann and Rheinwald, 1984) supplemented with 10% (v/v) fetal bovine serum (FBS) (Sigma). The final concentration of KGM components was ...
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bioRxiv - Immunology 2023Quote: Duodenal biopsies were collected in ice-cold RPMI-1640 and processed into single- cell suspensions by treatment with 2 mM EDTA in 2% (v/v) FBS/PBS followed by digestion with collagenase (Sigma) and cryopreservation as previously described.22 PBMCs were isolated from peripheral blood by density gradient centrifugation followed by cryopreservation ...
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bioRxiv - Neuroscience 2023Quote: ... unspecific epitopes were blocked with 100 μl phosphate buffered saline (PBS) containing 5% (v/v) goat serum (Sigma-Aldrich, #G6767) at RT for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Permeabilisation was achieved by incubation for 1h in freshly prepared blocking solution (PBS with 5% (v/v) normal donkey serum (D9663, Sigma) and 0.3% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... mesothelial cells were pelleted at 800g for 2 mins and resuspended in 50:50 v/v of RPMI (supplemented with 10% FBS, 1× penicillin/streptomycin, 2 μg/ml heparin (Sigma) and 1 μg/ml hydrocortisone (Sigma) ...
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bioRxiv - Bioengineering 2023Quote: ... the culture medium was supplemented with 10% (v/v) of AlamarBlue reagent solution at 0.1 mg mL−1 (#R7017, Sigma-Aldrich) and incubation was carried out for 2 hours at 37 °C before the fluorescence signal (Ex/Em = 530/590 ...
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bioRxiv - Bioengineering 2022Quote: ... To improve antibody specificity cells were incubated for 30 min at room temperature in a blocking solution consisting of 0.03% v/v Triton X-100 (Sigma, T8787) and 3% v/v bovine serum albumin (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... or a combination of two strains (3D7+1E4 or 3D7+1A5) using 10 mL of the spore suspension containing 0.1% (v/v) Tween-20 (Sigma Aldrich) or a mock solution (water and 0.1% (v/v ...
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bioRxiv - Plant Biology 2023Quote: ... samples were combined with 1.0 mL freshly prepared 5% (v/v) sulfuric acid in MeOH and 200 µL of toluene with 0.0005% (w/v) butylated hydroxy toluene (BHT; Sigma Aldritch) as well as 40 mg tripentadecanoin (15:0 ...