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Citations for Millipore Sigma :
3051 - 3100 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Cells were permeabilised with PBS/0.5 % Triton-X100 for 15m and blocked with PBS supplemented with 0.1 % (v/v) Triton-X100 (Sigma Aldrich), 1 % BSA fraction V (w/v ...
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bioRxiv - Developmental Biology 2021Quote: ... for 10 mins and then blocked solution consisting of PBS supplemented with 0.1 % (v/v) Triton-X100 (Sigma Aldrich), 5% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... Plates were sealed by piping a border of vaseline and 5% (v/v) mineral oil (Sigma, #M841-100 ml) and covered with a 22×22 Fisherbrand Cover Glass (# 12-541-B) ...
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bioRxiv - Neuroscience 2021Quote: ... Paclitaxel was prepared in 1 part vehicle (1:1 v/v Cremophor EL [EMD Millipore] and 200- proof ethanol) and 2 parts sterile saline (UPS) ...
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bioRxiv - Molecular Biology 2021Quote: ... Images of cells in phenol red-free RPMI media (Thermo, #11835030) supplemented with 10% v/v foetal bovine serum (FBS) and ciprofloxacin (0.01 mg/ml; Sigma) were acquired with a 10x/1.22 Plan Fluor (wound healing ...
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bioRxiv - Molecular Biology 2021Quote: ... All cell culture medium was supplemented with 10% v/v foetal bovine serum (FBS) and ciprofloxacin (0.01 mg/ml; Sigma) and cells were maintained at 37°C in a humidified atmosphere with 5% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... after which they diluted in water and spotted in 10-fold serial dilutions (undiluted to 10-5) onto YPD media containing 0.04% MMS (v/v; Sigma). Plates were photographed after a 2-day incubation at 30°C.
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bioRxiv - Cell Biology 2022Quote: ... Beads were attached on the surface of the PDMS using an APTES-Ethanol solution (5% v/v) (Sigma-Aldrich) to identify the surface of the gel ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were loaded into the cell inlet at 500,000 cells/ml in PBS containing 10% v/v Optiprep (Sigma) and were maintained in suspension using a magnetic micro stir bar placed in the tube ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed with TBS-Tween (0.01% v/v) prior to detection using enhanced chemiluminescent (ECL) reagent (Millipore) and imaged using a ChemiDoc Imaging System (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... single cell suspension made and resuspended in a wash solution of 10% v/v fetal calf serum (Sigma-Aldrich) in DMEM (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... from which debris and dead cells were removed by density gradient centrifugation using a 46% v/v Percoll (Sigma) gradient ...
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bioRxiv - Cell Biology 2019Quote: ... homozygous pans1-1 seeds were incubated for 17 h at room temperature in 5 mL of 0.3% (v/v) ethyl methanesulfonate (EMS) (SIGMA) with gentle agitation ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested after 16 hours and resuspended into 5 mL lysis buffer (200 mM Tris pH 8.0, 500 mM NaCl, 1% (v/v) Triton X-100 (Sigma), 0.02 mg/mL leupeptin ...
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bioRxiv - Bioengineering 2019Quote: ... were loaded into 2% (w/v) alginate solution and dropping into 100 mM CaCl2 solution containing 0.25% (w/v) chitosan (Sigma). Chitosan was initially dissolved at 4% within 0.066 M glacial acetic acid (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated for 1-3h in blocking solution (10% v/v Sigma-Aldrich sheep serum in PBS-T) at 21°C ...
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bioRxiv - Plant Biology 2019Quote: ... Seeds (15 per mutant line) were surface sterilized by rinsing in 5% (v/v) Sodium Hypochlorite (Sigma Aldrich, UK) for 10 mins and were rinsed with water three times before being imbibed in 1.75 mL of water for 5 days at 4°C to ensure uniform germination ...
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bioRxiv - Microbiology 2019Quote: ... catalase) (Becton Dickinson) and 0.05% v/v Tween 80 (7H9-OADC-Tw) plus 20 μg/mL kanamycin (Sigma-Aldrich) at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in 5 µL of cold lysis buffer (10 mM Tris-HCl [pH 7.4], 10 nM NaCl, 3 nM MgCl2, 0,1% v/v Igepal CA-360 [Sigma-Aldrich] and incubated on ice for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... Buffer B contained during protease cleavage 300 mM NaCl and 0.1% (v/v) CHAPS (Sigma-Aldrich, St Louis, MI). The HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was discarded and the membrane fractions were homogenized in 20 mL solubilization buffer (25 mM HEPES pH 7.5, 150 mM NaCl, 5% v/v glycerol) with cocktail inhibitors (Sigma) per initial liter of culture volume ...
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bioRxiv - Bioengineering 2020Quote: ... a gradient was prepared such that a 35% (v/v) 1:1 4-morpholinepropanesulfonic acid (MOPS):Opti-prep (Sigma) solution in HBSS was layered on top of a 20% (v/v ...
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bioRxiv - Bioengineering 2020Quote: ... All cells were cultured on tissue culture polystyrene dishes treated with 0.1% v/v gelatin solution (ES-006-B, Millipore) and were incubated in a humidified incubator at 37°C and 5% CO2 until they reached confluence ...
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bioRxiv - Bioengineering 2020Quote: ... we added 3% v/v photoinitiator Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (Sigma-Aldrich, “LAP”, 39.2 mg/mL in DI water). Produced Ln/polymer droplets were collected through Tygon tubing into a 24-well plate (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... After 3 times washing with PBS-T [PBS with 0.5% (v/v) Triton X-100 (Sigma Aldrich, # X-100)] fixed brains were incubated with primary antibody containing 10% Normal Donkey Serum in 0.4% PBS-T overnight at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... 0.1% solution of Triton X-100 in PBS (v/v) and 3% Bovine Serum Albumin solution (Sigma Aldrich, A3803) in PBS (w/v ...
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bioRxiv - Plant Biology 2020Quote: ... acetonitrile and 0.1% (v/v) formic acid in water following a further filtering with 0.22 µm centrifugal filter units (Millipore, USA) to remove any undissolved pellets ...
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bioRxiv - Microbiology 2020Quote: ... para as described above or with treated DMEM supplemented with 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich). To inhibit IRE1 nuclease and kinase activity ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: Mature anthers were harvested into 80% (v/v) EtOH and stained with a 1 in 20 dilution of Lugol’s iodine solution (Sigma) overnight ...
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bioRxiv - Plant Biology 2021Quote: ... followed by total protein isolation with extraction buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% [v/v] NP-40 and 1:200 protease inhibitor cocktail from Sigma). Co-IP was carried out the same as for co-IP with protoplasts except that GFP-Trap agarose beads (Chromotek ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed five times with TBS containing 0.05% v/v Tween-20 and polyclonal anti-human antibody conjugated to HRP (Sigma) added for one hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: Approximately 20 µL of cells were air-dried either on agarose pads (1% (w/v) in PBS) or on slides coated with 0.1% (w/v) poly-l-lysine solution (Sigma) and bacterial nucleoids stained by topping cells with a drop of Fluoroshield™ containing 1.5 µg/mL 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2021Quote: ... Borosilicate glass capillaries are loaded with a concentrated SARS-CoV-2 suspension previously coloured by the addition of 10% (V/V) of 0.5% phenol red in PBS (Sigma), then connected to a FemtoJet 4i microinjector (Eppendorf) ...
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bioRxiv - Microbiology 2021Quote: ... Laurdan microscopy was carried out with cells stained with 100 µM Laurdan (Sigma Aldrich; dissolved in 1% (v/v) DMF ...
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bioRxiv - Plant Biology 2021Quote: ... acetonitrile and 0.1% (v/v) formic acid in HPLC grade water followed by a filtering step using 0.22 μm centrifugal filters (Millipore). Purified peptide suspensions (2 μL each ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary (1:100) and secondary (1:200) antibodies were diluted in PBS containing 1% (v/v) BSA (Sigma-Aldrich). Primary antibodies were incubated overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... samples were fixed in 2.5% (v/v) glutaraldehyde in HBSS on IsoporeTM membrane TMTP filters with pore size 5 µm (Merck-Millipore) for 30 min followed by washing thrice with HBSS buffer (10 min each) ...
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bioRxiv - Cell Biology 2021Quote: ... in PBS) were performed and then proceeded to permeate and block overnight using 0.1% v/v Triton X-100 (Sigma), 0.1% v/v BSA and 10% v/v Normal Goat Serum (NGS ...
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bioRxiv - Cell Biology 2021Quote: ... on coverslips previously treated 30-40 minutes with a sterile bovine gelatin solution (0.1% v/v in PBS, Sigma) and incubated for 24 hours prior to irradiation ...
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bioRxiv - Cell Biology 2021Quote: ... TMT labeled samples for a given experiment were then pooled and acidified with 5% formic acid (Sigma, v/v). Samples were concentrated using a SpeedVac and resuspended in buffer A (95% water ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated with X-Gal staining solution (25% v/v citrate buffer, 5mM potassium ferrocyanide (Sigma-P3289), 5mM potassium ferricyanide (Sigma-P-8131) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transferred in a total volume of 0.5 μl into 2 μl of cell lysis buffer consisting of 0.2 % (v/v) Triton X-100 (Sigma Aldrich) and 2 U/μl RNase Inhibitor (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were incubated with 5% skimmed milk in PBS supplemented with 0.05% (v/v) Tween 20 (Sigma, PBS-T) for 30 minutes at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: ... 65% v/v acidified type I rat tail collagen (2.035mg/mL, First link, UK) and 10% v/v of 10X minimal essential medium (MEM, Sigma) were mixed and neutralised ...
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bioRxiv - Biophysics 2020Quote: The water phase was prepared by mixing two parts of acrylamide solution (40 % v/v, Sigma, St. Louis, USA) with one part of N,N’-methylenebisacrylamide solution (2 % v/v ...
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bioRxiv - Biophysics 2022Quote: ... Both the CS and cantilevers were washed with acetone and functionalized using 2% (v/v) 3-aminopropyltriethoxysilane (Millipore Sigma) solution dissolved in acetone ...
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bioRxiv - Bioengineering 2022Quote: ... A solution of PEGDA 700/575 at 16% v/v in ultrapure water with lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP; Sigma-Aldrich) at 0.1% wt/v was prepared ...
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bioRxiv - Biophysics 2022Quote: ... Non-specific protein adsorption to the coverslip was prevented by adding 0.005% (v/v) Tween-20 (P2287, Sigma-Aldrich) to the solution ...
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bioRxiv - Cell Biology 2022Quote: ... v/v) solution (0.5 ml) containing the following internal standards: C13 and N15 labeled amino acids standard mix (Sigma) was added to the tubes followed by centrifugation ...
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bioRxiv - Molecular Biology 2022Quote: ... embryos were washed twice on a petri dish using 1x PBS containing 0.1% (v/v) Triton X-100 (Sigma). Next ...