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Citations for Millipore Sigma :
3051 - 3100 of 4532 citations for Human LACC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: PC3 human prostate cancer cells (ATCC, CRL-1435) were cultured in HAM F12 medium (Sigma‒Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were grown on vitronectin coated plates containing PluriSTEM® Human ES/iPS Cell Medium (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... human Spindly siRNA (Sequence antisense: 5’-GAAAGGGUCUCAAACUGAA-3’, dTdT overhangs, Sigma-Aldrich, St. Louis, MO, USA), and control siRNA (D-001810-10-05 ...
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bioRxiv - Pathology 2024Quote: ... the cells were cultured on culture dishes coated with 0.05mg/ml human placenta derived COLIV (Sigma) in Bronchial epithelial cell growth medium with added supplements according to the manufacturer’s recommendation (BEBM-Lonza ...
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bioRxiv - Cancer Biology 2024Quote: Tumors were harvested as mice reached human endpoint and fixed in neutral buffered formalin (Sigma-Aldrich) for 24-48h ...
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bioRxiv - Immunology 2024Quote: ... 10 μg/mL dsDNA or 10 μg/mL recombinant human insulin (Sigma Aldrich, St. Louis, MO). After five washes in PBS with 0.05% Tween 20 ...
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bioRxiv - Neuroscience 2024Quote: Glycated Aβ (gAβ) was prepared by incubating 10 mg/ml of human Aβ1-42 (Sigma-Aldrich) with or without 0.5 mM methylglyoxal (MGO ...
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bioRxiv - Microbiology 2024Quote: Primary human foreskin fibroblasts (HFFs) (ATCC) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) with 4.5 g/L glucose ...
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bioRxiv - Immunology 2024Quote: ... Plates were then washed and incubated with HRP-conjugated goat anti-human IgG antibody (Sigma-Aldrich) (diluted 1:2500 in PBS-T ...
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bioRxiv - Molecular Biology 2024Quote: 50,000 human iPS-CM were seeded/well in Lab-Tek 4-well chamber slides (Sigma-Aldrich) and cultured for five days according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Human embryonic kidney 293 (HEK293) cells were cultured in RPMI-1640 medium (Sigma, St. Louis, MO) with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... HGF concentration in the CM was determined using the human HGF ELISA Kit (Sigma-Aldrich #RAB0212) following manufacturer protocol.
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bioRxiv - Biophysics 2020Quote: ... The plasmids for p53-R248Q were transformed into Rosetta™ 2 (DE3) (Sigma Aldrich, 71397) by electroporation ...
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bioRxiv - Genetics 2021Quote: ... 100 ug/mL blasticidin S (to kill cells not expressing the blasticidin-resistant plasmid; Sigma), and 10 nM AP1903 (to kill un-integrated cells expressing the AP1903-sensitive caspase gene ...
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bioRxiv - Biophysics 2022Quote: The pET-19b plasmids encoding the CPB constructs were transformed into BL21 (DE3) strain (Sigma) for protein over-expression ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg of each minigene plasmid was delivered to cells using branched polyethylenimine reagent (Sigma) in FBS-free DMEM ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR product were assembled into KpnI and EcoRI difgested pZDonor-AAVS1 Puromycin plasmid (Sigma-Aldrich) and ligated using the Quick Ligase Kit (New England Biolab) ...
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bioRxiv - Immunology 2019Quote: ... Component DNA plasmids were transfected into Lenti-X-HEK-293T cells via GeneJuice® (Novagen) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Each plate was transfected with 20 μg of overexpression plasmid with 100 μg PEI (Sigma). After 48 h cells were collected in TBS ...
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bioRxiv - Microbiology 2021Quote: ... Expression was induced from the plasmids in BL21 cell induced with 0.2 mM IPTG (Sigma) overnight at 18°C ...
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bioRxiv - Biochemistry 2020Quote: ... the GAF domain (residues 164–313) of RcaE was cloned into plasmid pET-28 (Novagen). The construct was expressed in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Neuroscience 2020Quote: ... A plasmid solution (DNA concentration of 1 μg/μl + Fast-Green 1% (F-7258, Sigma)) was then injected in the lateral ventricles of the embryos with glass micropipettes ...
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bioRxiv - Cell Biology 2021Quote: ... coli DH10B cells and cells harboring recombinant plasmids selected on Luria Bertani (LB; Sigma-Aldrich) agar plates with 50 μg/ml kanamycin ...
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bioRxiv - Immunology 2022Quote: ... The sequence was cloned into the pET42a plasmid (Cat No 70561, Novagen, Merck, Overijse, Belgium) in frame with an N-terminal caspase3-cleavable glutathione S-transferase (GST)-tag (26) ...
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bioRxiv - Biophysics 2022Quote: ... A mixture of 37.5 µg of ABCA7EQ plasmid and 75 µg of polyethyleneimine (PEI, Sigma) was incubated for 15 min at room temperature before being applied to 15 ml plates of HEK293T cells at a confluency of 60 - 80% to initiate transfection and expression ...
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bioRxiv - Microbiology 2022Quote: ... All plasmids were maintained as strains and maintained at -80°C in 25% glycerol (Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... Myc-CaP cells were transfected with pCas9-Rb1-CRISPR plasmid (Sigma-Aldrich, St. Louis, MO) using Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The pCMV-FLAG-His6-NPC1L1-MLD was cloned in the pFLAG-CMV-3 plasmid (Sigma). This construct contains a pre-protrypsin signal sequence ...
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bioRxiv - Neuroscience 2022Quote: ... A mixture of plasmid DNA (∼2 µg/µl) and 0.03 % fast green dye (Sigma Aldrich) was injected intraventricularly using micropipettes pulled from borosilicate glass capillaries (1.5 mm outer diameter ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was cloned into the NdeI and XhoI sites of plasmid pET21b (Novagen). To generate a plasmid for the expression of a cleavable GST tagged GbpA ...
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bioRxiv - Molecular Biology 2020Quote: A synthetic gene (Integrated DNA Technologies) for Nb806 was cloned into plasmid pET-26b(+) (Novagen) with a N-terminal His6 tag followed by a thrombin protease cleavage site ...
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bioRxiv - Neuroscience 2020Quote: ... and then 8-12 ug of plasmid mixed with Fast Green (2 mg/ml; Sigma) was injected into the lateral ventricle of embryos ...
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bioRxiv - Genetics 2021Quote: ... Lentiviral expression vectors were purified with GenElute HP Plasmid Maxiprep Kit (Sigma, St-Louis, MO) and only plasmid preparations with absorbance (260/280 ...
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bioRxiv - Cell Biology 2021Quote: ... 7.5μg of pSPAX2 and 4μg of pVSVG packaging plasmid using standard calcium precipitation protocol (Sigma) and grown in 20% serumcontaining medium for 24h ...
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bioRxiv - Biochemistry 2021Quote: ... and 100 ng of pcDNA A3C-3xHA expression plasmid using GeneJuice transfection reagent (EMD Millipore). The SIVsmm lineage 5 (L5 ...
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bioRxiv - Neuroscience 2022Quote: A mix of endotoxin-free plasmid preparation (0.5 mg/mL) and 0.5% Fast Green (Sigma) was injected into one lateral hemisphere of E15.5 embryos using FemtoJet 4i (Eppendorf) ...
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bioRxiv - Neuroscience 2022Quote: A mix of endotoxin-free plasmid preparation (2 mg/mL) and 0.5% Fast Green (Sigma) mixture was injected using FemtoJet 4i (Eppendorf ...
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bioRxiv - Microbiology 2024Quote: ... 293T cells were transfected with gene drive plasmids precomplexed with PEI (408727-100ML; Sigma[Aldrich), cultured for 12 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... and the RDF plasmid encoding the RD114 envelope) using the GeneJuice transfection reagent (Merck Millipore). Viral supernatant was collected 48 and 72 hours after transfection ...
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bioRxiv - Biochemistry 2024Quote: HEK-293T cells were transfected with indicated plasmids using GeneJuice (70967, Novagen, San Diego, CA) and Opti-MEM (51985 ...
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bioRxiv - Cancer Biology 2023Quote: Other plasmids used in the study were: pLKO.1-puro-CMV-TurboGFP (Sigma-Aldrich, SHC003), pLenti-C-mGFP-P2A-puro-FGF19 (Origene ...
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bioRxiv - Cell Biology 2023Quote: The LEL-Ov-TSP-3 sequence was cloned into plasmid pET32a (Novagen, Madison, WI, USA) which was deployed to transform BL21DE32 strain E ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μg of concentrated DNA plasmid mixtures (1 μg/μL containing 0.05% Fast Green (Sigma)) was injected into the 4th ventricle of E13.5 mouse embryos using a pulled glass capillary pipette ...
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bioRxiv - Cancer Biology 2023Quote: ... OC cells were transfected with lentivirus based CRISPR plasmid (MISSION® gRNA, HSPD0000035812, Sigma Aldrich) targeting human RelA gene (sgRNA sequence ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmid DNA was extracted from 100 mL cultures using a midi-prep kit (Millipore Sigma) and the DNA concentration was determined by NanoDrop spectrometer ND-1000.
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bioRxiv - Neuroscience 2023Quote: ... These fragments were ligated into the cloning sites of the plasmid vector pET-21b (Novagen) between NdeI and XhoI ...
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bioRxiv - Molecular Biology 2024Quote: ... were expressed in Escherichia coli BL21 (DE3) slyD- cells [56] carrying the plasmid pRARE2 (Novagen). Recombinant GST (vector pGEX-4T2 (Amersham) ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with 1/200 diluted Goat Anti human IgG Fc-FITC antibody (Cat: F9512, Sigma) for 45 min and analyzed using flow cytometry (CytoFLEX ...
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bioRxiv - Biophysics 2020Quote: ... then immersed in a solution of Human Plasma Fibronectin (10 ug/mL in PBS; #FC010, EMD Millipore), and washed thoroughly in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were probed with antibody specific for human P2X4 (Cat. No. HPA039494, Sigma-Aldrich, St. Louis, MO), mouse P2X4 (Cat ...